Rh3BG085100

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005159
Physical Location & Seq
Forward (+)
6638899 .. 6640377
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG085100.1

Sequence Viewer

Length: 528 bp
ATGATCCAAGGAGGGGATTTCACTGAAGGAGATGGAACTGGAGGGATCAGTATCTATGGTTCCAAATTTGATGATGAGAGCTTTGCTTTGAAGCATGTTGGGCCTGGTGTATTGAGCATGGCTAATGCAGGCCCTGGTACTAATGGGAGTCAGTTTTTAATCGGCACAGTAAAGGAAAATGATCCTACAAGGAGGAAGTTGACTGATCAGCAAGCTTTCCAAATTTCAAAGATGCCTGGAGTTGTTTCGGTTTTTCCCAACTCGAAAAGGATAAATGTCATTGTCGGTTTCATTGATACTGGAATTTGGCCTGAATCTCCAAGTTTTAATGATGACAACATGCCTCCAGTGCCAGCTAGATGGAAGGGGGTTTGTCAATCAGGAGAGGCATTCAATGCTTCAACTTGCAACAGTGATTGGGGCTATACTATAAGTCCGGTTATGAAGCTGAGGAAGATTCAGCGGACACAGTGGCATTCAGTTCTCCAAGGGACAGTGCCGGTCATGGCAGCCACACAGCCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

175

Amino Acids

18.86

Weight (kDa)

6.72

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 1 - 61 1.5e-10 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 463
AclWI GGATC 2 cut(s) 53, 176
AcsI RAATTY 3 cut(s) 65, 222, 303
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 1 cut(s) 139
AfiI CCNNNNNNNGG 1 cut(s) 13
AgsI TTSAA 4 cut(s) 91, 228, 394, 402
AjnI CCWGG 3 cut(s) 103, 133, 235
AluBI AGCT 4 cut(s) 81, 215, 356, 448
AluI AGCT 4 cut(s) 81, 215, 356, 448
AlwI GGATC 2 cut(s) 53, 176
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 3 cut(s) 101, 130, 308
ApeKI GCWGC 1 cut(s) 509
ApoI RAATTY 3 cut(s) 65, 222, 303
AspS9I GGNCC 2 cut(s) 101, 131
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 1 cut(s) 521
BccI CCATC 2 cut(s) 26, 354
BciT130I CCWGG 3 cut(s) 105, 135, 237
BclI TGATCA 1 cut(s) 205
BfaI CTAG 1 cut(s) 357
BfmI CTRYAG 1 cut(s) 524
BisI GCNGC 1 cut(s) 510
BlsI GCNGC 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 105, 135, 237
BmgT120I GGNCC 2 cut(s) 101, 131
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 3 cut(s) 105, 135, 237
BmsI GCATC 1 cut(s) 222
BpmI CTGGAG 3 cut(s) 60, 258, 330
Bpu10I CCTNAGC 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 7, 133, 487
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 13
Bse118I RCCGGY 1 cut(s) 499
Bse1I ACTGG 3 cut(s) 43, 304, 347
Bse8I GATNNNNATC 1 cut(s) 50
BseBI CCWGG 3 cut(s) 105, 135, 237
BseDI CCNNGG 3 cut(s) 7, 133, 487
BseJI GATNNNNATC 1 cut(s) 50
BseLI CCNNNNNNNGG 1 cut(s) 13
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 3 cut(s) 43, 304, 347
BseXI GCAGC 1 cut(s) 521
BshFI GGCC 3 cut(s) 103, 132, 310
BsiSI CCGG 2 cut(s) 437, 500
BslFI GGGAC 1 cut(s) 505
BslI CCNNNNNNNGG 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 505
BsmI GAATGC 2 cut(s) 389, 475
BsnI GGCC 3 cut(s) 103, 132, 310
Bsp143I GATC 4 cut(s) 3, 45, 181, 205
BspACI CCGC 1 cut(s) 463
BspANI GGCC 3 cut(s) 103, 132, 310
BspCNI CTCAG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 2 cut(s) 53, 176
BsrFI RCCGGY 1 cut(s) 499
BsrI ACTGG 3 cut(s) 43, 304, 347
BssAI RCCGGY 1 cut(s) 499
BssECI CCNNGG 3 cut(s) 7, 133, 487
BssMI GATC 4 cut(s) 3, 45, 181, 205
BssT1I CCWWGG 2 cut(s) 7, 487
Bst2UI CCWGG 3 cut(s) 105, 135, 237
Bst4CI ACNGT 4 cut(s) 169, 413, 471, 496
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 3 cut(s) 130, 213, 354
BstDEI CTNAG 1 cut(s) 449
BstKTI GATC 4 cut(s) 6, 48, 184, 208
BstMBI GATC 4 cut(s) 3, 45, 181, 205
BstMWI GCNNNNNNNGC 3 cut(s) 100, 349, 395
BstNI CCWGG 3 cut(s) 105, 135, 237
BstNSI RCATGY 2 cut(s) 98, 343
BstSCI CCNGG 3 cut(s) 103, 133, 235
BstSFI CTRYAG 1 cut(s) 524
BstV1I GCAGC 1 cut(s) 521
BstXI CCANNNNNNTGG 1 cut(s) 360
BsuRI GGCC 3 cut(s) 103, 132, 310
BtsIMutI CAGTG 5 cut(s) 21, 354, 418, 476, 501
Cac8I GCNNGC 3 cut(s) 130, 213, 354
CaiI CAGNNNCTG 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 2 cut(s) 101, 131
Csp6I GTAC 1 cut(s) 138
CviAII CATG 4 cut(s) 95, 118, 340, 505
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 1 cut(s) 449
DpnI GATC 4 cut(s) 5, 47, 183, 207
DpnII GATC 4 cut(s) 3, 45, 181, 205
Eco130I CCWWGG 2 cut(s) 7, 487
Eco57I CTGAAG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 103, 133, 235
EcoT14I CCWWGG 2 cut(s) 7, 487
ErhI CCWWGG 2 cut(s) 7, 487
FaeI CATG 4 cut(s) 98, 121, 343, 508
FaiI YATR 9 cut(s) 57, 96, 119, 341, 426, 431, 443, 506, 526
FaqI GGGAC 1 cut(s) 505
FatI CATG 4 cut(s) 94, 117, 339, 504
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 1 cut(s) 510
Fsp4HI GCNGC 1 cut(s) 510
FspBI CTAG 1 cut(s) 357
GluI GCNGC 1 cut(s) 510
GsuI CTGGAG 3 cut(s) 60, 258, 330
HaeIII GGCC 3 cut(s) 103, 132, 310
HapII CCGG 2 cut(s) 437, 500
Hin1II CATG 4 cut(s) 98, 121, 343, 508
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 3 cut(s) 148, 314, 457
HpaII CCGG 2 cut(s) 437, 500
Hpy166II GTNNAC 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 381
Hpy8I GTNNAC 1 cut(s) 201
HpyAV CCTTC 2 cut(s) 20, 358
HpyCH4III ACNGT 4 cut(s) 169, 413, 471, 496
HpyCH4V TGCA 2 cut(s) 128, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 349, 395
HpyF3I CTNAG 1 cut(s) 449
Hsp92II CATG 4 cut(s) 98, 121, 343, 508
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 4 cut(s) 3, 45, 181, 205
Lsp1109I GCAGC 1 cut(s) 521
LweI GCATC 1 cut(s) 222
MaeI CTAG 1 cut(s) 357
MalI GATC 4 cut(s) 5, 47, 183, 207
MboI GATC 4 cut(s) 3, 45, 181, 205
MboII GAAGA 1 cut(s) 466
MluCI AATT 3 cut(s) 65, 222, 303
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 6 cut(s) 5, 35, 186, 354, 379, 444
MseI TTAA 2 cut(s) 158, 327
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 2 cut(s) 437, 500
MspR9I CCNGG 3 cut(s) 105, 135, 237
Mva1269I GAATGC 2 cut(s) 389, 475
MvaI CCWGG 3 cut(s) 105, 135, 237
MwoI GCNNNNNNNGC 3 cut(s) 100, 349, 395
NdeII GATC 4 cut(s) 3, 45, 181, 205
NlaIII CATG 4 cut(s) 98, 121, 343, 508
NlaIV GGNNCC 1 cut(s) 61
NspI RCATGY 2 cut(s) 98, 343
PctI GAATGC 2 cut(s) 389, 475
PfeI GAWTC 2 cut(s) 314, 457
PkrI GCNGC 1 cut(s) 511
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Psp6I CCWGG 3 cut(s) 103, 133, 235
PspGI CCWGG 3 cut(s) 103, 133, 235
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 101, 131
PsrI GAACNNNNNNTAC 2 cut(s) 43, 75
PstNI CAGNNNCTG 1 cut(s) 134
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 158, 327
SatI GCNGC 1 cut(s) 510
Sau3AI GATC 4 cut(s) 3, 45, 181, 205
Sau96I GGNCC 2 cut(s) 101, 131
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 3 cut(s) 105, 135, 237
SetI ASST 4 cut(s) 83, 217, 358, 450
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 524
Sse9I AATT 3 cut(s) 65, 222, 303
SsiI CCGC 1 cut(s) 463
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 3 cut(s) 103, 133, 235
StyI CCWWGG 2 cut(s) 7, 487
TaaI ACNGT 4 cut(s) 169, 413, 471, 496
TaqI TCGA 1 cut(s) 263
TasI AATT 3 cut(s) 65, 222, 303
TfiI GAWTC 2 cut(s) 314, 457
Tru1I TTAA 2 cut(s) 158, 327
Tru9I TTAA 2 cut(s) 158, 327
TscAI CASTG 5 cut(s) 28, 354, 418, 476, 501
TseI GCWGC 1 cut(s) 509
TspDTI ATGAA 2 cut(s) 280, 458
TspRI CASTG 5 cut(s) 28, 354, 418, 476, 501
XapI RAATTY 3 cut(s) 65, 222, 303
XceI RCATGY 2 cut(s) 98, 343
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.