Rh3BG283800

26S proteasome non-ATPase regulatory subunit 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003773
Physical Location & Seq
Reverse (-)
28177818 .. 28179897
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG283800.1

Sequence Viewer

Length: 1191 bp
ATGCCGTCTACTGGCTGGATAAATAGTGTAGTATACACAGCAACTGTTTTTGCTAATGCAATTATGCATGCTGGGACTAGTATAACGACATTTATTGAAGAGGACCAGAACTGGTTTGCCAGAGCGGAAGGTTGGGCAGATTTCGGTATAACAGCTGGGCTAGGTGTTATTTACAGAGGCCAATCACAGCGTGGAAGATCTCTGATGGCACCTTTCTTGCCTCAGGGTGGGTCACATCACCGTCCAATACCATTTGTAGAAGGTGGAGCTCTCTATGCTATAGGCCTCATTCATGGCACCCGTAATGAGGACATCTCTGAATTCCTCATTCATGCCGCCCATAATGAGCTGATCACCGAATTCCTTGTTGAGAACCTACGTCACACTGATGTGCAGATCATTCAACATGGGGCATGCCTGGGTCTTGGCTTATCAGCTCTAGGAACTGCCGATGAGAACATTTATAATGAGATTAAAAATGTGCTTCTCTCTGACAGTGCTGTTTCTGGTGAAGCTGTCGGGATTAGCATGGGTTTACTCATGGTTGGAACTGTAAGTGAGAAGGCTAGTGAGATGCTTGCTTATGCACATGAAACACGAGGAAAAGACAATCAGGGGATTGGCATTGGGTATTGCCCTAACTATAAGATGGCTCAAATTGTCTCCCTGCTATCTAACTCCTACAACCCACATGTTCGATACGGTGCTGCTCTTGCAGTAGGAATTTCATGTGGTGGTACTGGCTTTAGTGAAGCCATATCTTTGTTAGAACCTTTGACTTCAGATGATGTTGATTTTGTACGCCAGGGTGCCCTCATTGCAATGGCTATGGTCATGATCCAGATTAGTGAAGCTAGTGATTCTCGTGTTGGTGCTTTTAGGCGGCAATTGGAAGAAATAATTCTTTATAACCATAATGATACCATGAGCAAAATGGGAGCAATCTTGGCCTCTGGAATTCTTGATGCTGGTGGAAGAAATGTGACTATAAGACTGCTTTCTAAGTCAAAACATGATAAAGTCACTGCAGTAATTGGCCTTGCTGTTTTCAGCCAATTTTGGTACTGGTATCCCCTCGTATATTTCTTAAGCTTGGCGTTCTCACCAACAGCTTTGATAGGACTGAACTATGATCTTAGAGTTCCGAAGTTTAGTTTCCTGTCGCATGCAAAACCTTCTCTCTTTGAATAG

Protein Analysis

396

Amino Acids

42.91

Weight (kDa)

5.7

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPN2_C PF18004 370 - 396 2.9e-06 26S proteasome regulatory subunit RPN2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 465, 909
AccB1I GGYRCC 3 cut(s) 208, 296, 809
AccBSI CCGCTC 1 cut(s) 125
AccI GTMKAC 2 cut(s) 8, 33
AciI CCGC 3 cut(s) 125, 336, 883
AclWI GGATC 1 cut(s) 832
AcsI RAATTY 4 cut(s) 320, 359, 723, 957
AcuI CTGAAG 1 cut(s) 765
AdeI CACNNNGTG 1 cut(s) 191
AfaI GTAC 3 cut(s) 739, 801, 1064
AfiI CCNNNNNNNGG 3 cut(s) 11, 227, 307
AflII CTTAAG 1 cut(s) 1087
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 3 cut(s) 98, 404, 1187
AhlI ACTAGT 1 cut(s) 77
AjnI CCWGG 2 cut(s) 417, 804
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 8 cut(s) 155, 269, 349, 437, 515, 854, 1092, 1112
AluI AGCT 8 cut(s) 155, 269, 349, 437, 515, 854, 1092, 1112
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 1 cut(s) 832
AlwNI CAGNNNCTG 1 cut(s) 44
AoxI GGCC 4 cut(s) 178, 283, 948, 1036
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 4 cut(s) 320, 359, 723, 957
Asp700I GAANNNNTTC 1 cut(s) 900
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 4 cut(s) 230, 346, 521, 1095
AvaII GGWCC 1 cut(s) 103
AxyI CCTNAGG 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 814
BanI GGYRCC 3 cut(s) 208, 296, 809
BanII GRGCYC 1 cut(s) 271
BauI CACGAG 2 cut(s) 597, 864
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 694
BccI CCATC 2 cut(s) 199, 643
BciT130I CCWGG 2 cut(s) 419, 806
BciVI GTATCC 1 cut(s) 1080
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 667
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 5 cut(s) 78, 161, 440, 567, 855
BfmI CTRYAG 2 cut(s) 279, 1026
BfrI CTTAAG 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 1080
BglII AGATCT 1 cut(s) 197
BisI GCNGC 3 cut(s) 336, 708, 884
BlsI GCNGC 3 cut(s) 337, 709, 885
Bme1390I CCNGG 2 cut(s) 419, 806
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 3 cut(s) 210, 298, 811
BmrFI CCNGG 2 cut(s) 419, 806
BmsI GCATC 2 cut(s) 564, 955
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BsaJI CCNNGG 2 cut(s) 418, 805
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 227, 307
Bse1I ACTGG 4 cut(s) 16, 116, 745, 1070
Bse21I CCTNAGG 1 cut(s) 222
Bse3DI GCAATG 2 cut(s) 816, 828
BseBI CCWGG 2 cut(s) 419, 806
BseDI CCNNGG 2 cut(s) 418, 805
BseLI CCNNNNNNNGG 3 cut(s) 11, 227, 307
BseMI GCAATG 2 cut(s) 816, 828
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 4 cut(s) 16, 116, 745, 1070
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 1 cut(s) 694
BseYI CCCAGC 2 cut(s) 71, 155
BsgI GTGCAG 1 cut(s) 413
BshFI GGCC 4 cut(s) 180, 285, 950, 1038
BshNI GGYRCC 3 cut(s) 208, 296, 809
BsiHKAI GWGCWC 1 cut(s) 271
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 3 cut(s) 11, 227, 307
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 4 cut(s) 180, 285, 950, 1038
Bsp1286I GDGCHC 2 cut(s) 271, 814
Bsp143I GATC 5 cut(s) 197, 351, 396, 837, 1132
BspACI CCGC 3 cut(s) 125, 336, 883
BspANI GGCC 4 cut(s) 180, 285, 950, 1038
BspCNI CTCAG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 210, 298, 811
BspMAI CTGCAG 1 cut(s) 1030
BspPI GGATC 1 cut(s) 832
BspT107I GGYRCC 3 cut(s) 208, 296, 809
BspTI CTTAAG 1 cut(s) 1087
BsrBI CCGCTC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 816, 828
BsrI ACTGG 4 cut(s) 16, 116, 745, 1070
BssECI CCNNGG 2 cut(s) 418, 805
BssMI GATC 5 cut(s) 197, 351, 396, 837, 1132
BssNAI GTATAC 1 cut(s) 34
BssSI CACGAG 2 cut(s) 597, 864
Bst1107I GTATAC 1 cut(s) 34
Bst2BI CACGAG 2 cut(s) 597, 864
Bst2UI CCWGG 2 cut(s) 419, 806
Bst4CI ACNGT 5 cut(s) 46, 242, 497, 553, 704
Bst6I CTCTTC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 1087
BstC8I GCNNGC 4 cut(s) 69, 415, 579, 1167
BstDEI CTNAG 3 cut(s) 222, 1002, 1136
BstKTI GATC 5 cut(s) 200, 354, 399, 840, 1135
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 5 cut(s) 197, 351, 396, 837, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 275, 713, 818, 947
BstNI CCWGG 2 cut(s) 419, 806
BstNSI RCATGY 4 cut(s) 71, 417, 695, 1169
BstSCI CCNGG 2 cut(s) 417, 804
BstSFI CTRYAG 2 cut(s) 279, 1026
BstSLI GKGCMC 1 cut(s) 814
BstV1I GCAGC 1 cut(s) 694
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BstZ17I GTATAC 1 cut(s) 34
Bsu36I CCTNAGG 1 cut(s) 222
BsuI GTATCC 1 cut(s) 1080
BsuRI GGCC 4 cut(s) 180, 285, 950, 1038
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 3 cut(s) 384, 502, 1023
Cac8I GCNNGC 4 cut(s) 69, 415, 579, 1167
CaiI CAGNNNCTG 1 cut(s) 44
CciI TCATGA 1 cut(s) 834
Cfr13I GGNCC 1 cut(s) 103
Csp6I GTAC 3 cut(s) 738, 800, 1063
CviQI GTAC 3 cut(s) 738, 800, 1063
DdeI CTNAG 3 cut(s) 222, 1002, 1136
DpnI GATC 5 cut(s) 199, 353, 398, 839, 1134
DpnII GATC 5 cut(s) 197, 351, 396, 837, 1132
DraIII CACNNNGTG 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 765
Eco81I CCTNAGG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 269
EcoRI GAATTC 3 cut(s) 320, 359, 957
EcoRII CCWGG 2 cut(s) 417, 804
EcoT22I ATGCAT 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 271
FaqI GGGAC 1 cut(s) 88
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 2 cut(s) 8, 33
Fnu4HI GCNGC 3 cut(s) 336, 708, 884
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 3 cut(s) 336, 708, 884
FspBI CTAG 5 cut(s) 78, 161, 440, 567, 855
GluI GCNGC 3 cut(s) 336, 708, 884
GsaI CCCAGC 2 cut(s) 75, 159
HaeIII GGCC 4 cut(s) 180, 285, 950, 1038
HindIII AAGCTT 1 cut(s) 1090
HinfI GANTC 1 cut(s) 860
HphI GGTGA 4 cut(s) 230, 346, 521, 1095
Hpy166II GTNNAC 3 cut(s) 9, 34, 536
Hpy188I TCNGA 5 cut(s) 204, 319, 493, 784, 1146
Hpy188III TCNNGA 5 cut(s) 520, 835, 841, 954, 962
Hpy8I GTNNAC 3 cut(s) 9, 34, 536
HpyAV CCTTC 4 cut(s) 122, 254, 556, 1185
HpyCH4III ACNGT 5 cut(s) 46, 242, 497, 553, 704
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 8 cut(s) 59, 67, 394, 587, 716, 821, 1028, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 713, 818, 947
HpyF3I CTNAG 3 cut(s) 222, 1002, 1136
HpySE526I ACGT 1 cut(s) 379
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 197, 351, 396, 837, 1132
LmnI GCTCC 2 cut(s) 266, 938
Lsp1109I GCAGC 1 cut(s) 694
LweI GCATC 2 cut(s) 564, 955
MaeI CTAG 5 cut(s) 78, 161, 440, 567, 855
MaeII ACGT 1 cut(s) 379
MaeIII GTNAC 4 cut(s) 231, 380, 982, 1021
MalI GATC 5 cut(s) 199, 353, 398, 839, 1134
MbiI CCGCTC 1 cut(s) 125
MboI GATC 5 cut(s) 197, 351, 396, 837, 1132
MboII GAAGA 4 cut(s) 110, 207, 905, 987
MfeI CAATTG 1 cut(s) 887
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 271, 814
MmeI TCCRAC 1 cut(s) 526
Mph1103I ATGCAT 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 900
MseI TTAA 2 cut(s) 474, 1088
MslI CAYNNNNRTG 3 cut(s) 387, 389, 821
MspA1I CMGCKG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 1087
MspR9I CCNGG 2 cut(s) 419, 806
MunI CAATTG 1 cut(s) 887
MvaI CCWGG 2 cut(s) 419, 806
MwoI GCNNNNNNNGC 4 cut(s) 275, 713, 818, 947
NdeII GATC 5 cut(s) 197, 351, 396, 837, 1132
NlaIV GGNNCC 3 cut(s) 210, 298, 811
NmuCI GTSAC 4 cut(s) 231, 380, 982, 1021
NsiI ATGCAT 1 cut(s) 69
NspI RCATGY 4 cut(s) 71, 417, 695, 1169
OliI CACNNNNGTG 1 cut(s) 389
PaeI GCATGC 3 cut(s) 71, 417, 1169
PagI TCATGA 1 cut(s) 834
PceI AGGCCT 1 cut(s) 285
PciI ACATGT 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 1 cut(s) 860
PkrI GCNGC 3 cut(s) 337, 709, 885
PscI ACATGT 1 cut(s) 691
PsiI TTATAA 2 cut(s) 465, 909
Psp124BI GAGCTC 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 417, 804
PspFI CCCAGC 2 cut(s) 71, 155
PspGI CCWGG 2 cut(s) 417, 804
PspN4I GGNNCC 3 cut(s) 210, 298, 811
PspPI GGNCC 1 cut(s) 103
PstI CTGCAG 1 cut(s) 1030
PstNI CAGNNNCTG 1 cut(s) 44
PsuI RGATCY 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 3 cut(s) 739, 801, 1064
RsaNI GTAC 3 cut(s) 738, 800, 1063
RseI CAYNNNNRTG 3 cut(s) 387, 389, 821
SacI GAGCTC 1 cut(s) 271
SaqAI TTAA 2 cut(s) 474, 1088
SatI GCNGC 3 cut(s) 336, 708, 884
Sau3AI GATC 5 cut(s) 197, 351, 396, 837, 1132
Sau96I GGNCC 1 cut(s) 103
ScrFI CCNGG 2 cut(s) 419, 806
SduI GDGCHC 2 cut(s) 271, 814
SfaNI GCATC 2 cut(s) 564, 955
SfcI CTRYAG 2 cut(s) 279, 1026
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 3 cut(s) 387, 389, 821
SmlI CTYRAG 1 cut(s) 1087
SmoI CTYRAG 1 cut(s) 1087
SpeI ACTAGT 1 cut(s) 77
SphI GCATGC 3 cut(s) 71, 417, 1169
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 3 cut(s) 125, 336, 883
SspMI CTAG 5 cut(s) 78, 161, 440, 567, 855
SstI GAGCTC 1 cut(s) 271
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 417, 804
TaaI ACNGT 5 cut(s) 46, 242, 497, 553, 704
TaiI ACGT 1 cut(s) 382
TaqI TCGA 1 cut(s) 697
TauI GCSGC 2 cut(s) 338, 886
TfiI GAWTC 1 cut(s) 860
Tru1I TTAA 2 cut(s) 474, 1088
Tru9I TTAA 2 cut(s) 474, 1088
TscAI CASTG 3 cut(s) 391, 502, 1030
TseFI GTSAC 4 cut(s) 231, 380, 982, 1021
TseI GCWGC 1 cut(s) 707
Tsp45I GTSAC 4 cut(s) 231, 380, 982, 1021
TspDTI ATGAA 4 cut(s) 281, 320, 606, 717
TspRI CASTG 3 cut(s) 391, 502, 1030
Vha464I CTTAAG 1 cut(s) 1087
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 4 cut(s) 320, 359, 723, 957
XceI RCATGY 4 cut(s) 71, 417, 695, 1169
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 931
XmiI GTMKAC 2 cut(s) 8, 33
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 5 cut(s) 78, 161, 440, 567, 855
Zsp2I ATGCAT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.