Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0003773

Physical Location & Seq

Genomic Coordinates Forward (+)

1427588 .. 1435775

Gene Structure 8188 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG009100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2751 bp

ATGGCGACCTTAGTGAGCTCGGCAGGTGGTCTACTTGCCATGCTGAACGAGTCCCGTCCGGAGCTAAAGCTCTACGCTTTGTCCAACCTCAACAAATTGGTTGATGGGTTCTGGCCCGAGATCTCCACCAGCGTCCCCATCATAGAAAGTTTGTATGAAGATGAAGAGTTTGGTCAGCATCAAAGACAACTGGCTGCATTGCTTGTTTCAAAGGTTTTCTACTATTTGGGTGAACTTAATGACTCACTGTCCTACGCCCTTGGAGCTGGATCCTTATTTGACGTGTCAGAAGATTCTTATTATATTCATACTCTTCTTGCTAAAGCTATAGATGAGTATGCCAGTCTTAAGTCTAAGGCAGCAGAATCAAATGCTGAAGCAGCAAATGTGGATCCTAGGTTGGAGGCTATCGTGGAGAGAATGCTGAATAAGTGTATCCTGGATGGGAAATATCAACAAGCTATGGGGATTGCAATTGAATGTCGAAGACTGGATAAACTTGAGGAAGCAATCATAAAGAGTGATAATGTTCAAGGAACCCTGTCATATTGCATCAATGTCTCTCATTCATATATTAATCTTAGAGAATACAGACGTGAGGTGCTTCGTCTTCTTGTCAGAGTGTATCAAAAGCTGCCTTCTCCAGATTACTTGAGCATTTGTCAGTGTCTCATGTTCTTGGATGAACCAGAAGGTGTTGCAACCATATTGGAGAAGCTCCTGCGCTCTGAAAACAAGGAAGATGCACTTTTGGCATTTCAAATAGCCTTTGACCTTGTTGAGAATGAGCACCAAGCTTTTCTCTTAAATGTAAGAAATCGCCTTCCAGCTCCCAAGACTAAAACCTCAGAGCCAGTGCAACCAGAATCCAGTGAAGCTGCACAAAATGAAGGTTCTGCTCCAGGGGATGTTCAAATGACAGATGGAAGTTCTACTACCAGTGTGACTGTGCCTGAAGATCCAAGTGAAGTGATGTATTCCGAGAGACTGACAAAAATCAAAGGAATTTTGTCCGGAGAGACGTCGATAAAGTTGACCTTACAATTTCTGTACAGCCATAACAAGTCTGATCTGCTTATTCTGAAGACAATAAAGCAGTCTATTGAGATGAGAAATAGTGTATGCCATAGTGCAACAATTTATGCTAATGCAATTATGCACGCTGGAACAACTGTGGATACATTTCTGAGGGAGAATCTGGACTGGTTAAGCAGAGCCACTAATTGGGCAAAATTCAGTGCAACAGCGGGGCTAGGTGTTATTCACAGAGGCCACTTGCAGCAGGGAAGATCTCTGATGGCACCTTACTTGCCTCAGGGTGGGGCTGGTGGTGGTGGAAGTCCGTACTCGGAAGGTGGTGCTCTGTATGCTCTGGGACTCATTCATGCCAACCATGGCGAGGGCATCAAACAGTTCCTTCGTGATAGCCTACGCAGTACTAATGTGGAGGTTATTCAACATGGGGCGTGCTTGGGTCTTGGCTTGTCAGCTTTAGGAACTGCTGATGAAGAGATTTATGATGACATTAAAAGTGTGCTGTATACTGACAGTGCCGTTGCTGGTGAAGCAGCTGGTATTAGTATGGGTTTACTCATGGTTGGAACTGCAAGTGAGAAGGCTAGTGAAATGCTTGCATATGCACATGAGACACAACATGAAAAGATCATCAGAGGGTTGGCATTGGGGATTGCCCTGACGGTATATGGAAGAGAAGAGGAAGCAGACACGCTAATTGAACAGATGACAAGAGATCAAGACCCCATATTGCGTTATGGTGGTATGTATGCATTGGCATTGGCCTATAGCGGGACAGCAAACAACAAAGCTATTCGCCAGCTGCTGCATTTTGCTGTGTCAGATGTGAGTGATGATGTTAGGAGAACTGCTGTTCTAGCCCTTGGTTTTGTCTTGTACTCTGAGCCAGAGCAGACGCCTCGTATTGTCTCCCTGCTATCTGAGTCCTACAACCCACATGTTCGATATGGTGCTGCTCTTGCAGTAGGAATTTCGTGTGCTGGTACTGGCTTGAGTGAGGCCATATCTTTGTTAGAGCCTCTGACTTCAGATGTTGTTGATTTTGTGCGCCAAGGTGCCCTCATTGCAATGGCTATGGTCATGGTCCAGATTAGTGAAGCTAGTGATTCTCGTGTTGGAGCTTTTAGGCGGCAATTGGAGAAAATAATTCTTGATAAGCATGAGGATACCATGAGCAAAATGGGAGCAATCTTGGCCTCTGGAATTCTTGACGCCGGCGGAAGGAATGTGACAATAAGACTACTTTCTAAGACAAAACATGATAAAGTCACTGCAGTGGTTGGCCTTGCTGTTTTCAGCCAATTTTGGTACTGGTATCCCCTCATATATTTCTTGAGCTTGGCATTCTCACCAACAGCTTTTATAGGACTGAACTATGACCTGAAAGTTCCGAAGTTTCAGTTCCTGTCATATGCAAAACCTTCTCTGTTTGAATATCCTAAACCGACCACTGTGGCAACCACTACATCGGCCGTGAAACTCCCCACTGCTGTTCTGTCAACATCAGCAAAGGCCACCAAAGCTAGGGCCAAGAAAGAAGCTGCAGATCAGAAGGCCAATCCTGAAAAGACAAATCCTGGGGGAGAGACATCTTCAGGAAAGGGAAAATCTTCTAGTGAGAAAGATGGGGATTCAATGCAGGTAAACCAATATGGGCATAATAATTCCAGCATTTTCTGTACTTCCTCCTTTCTGTTTTTGTCCGCATTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0019538 GO:0019941 GO:0022624 GO:0030163 GO:0031597 GO:0032991 GO:0034515 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0051603 GO:0071704 GO:0071944 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

PC_rep

Protein Analysis

916

Amino Acids

99.81

Weight (kDa)

5.54

Isoelectric Point (pI)

41.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPN2_N	PF21505	7 - 354	1.2e-89	26S proteasome subunit RPN2, N-terminal domain
PC_rep	PF01851	488 - 521	7.4e-06	Proteasome/cyclosome repeat
HEAT_2	PF13646	611 - 703	1.8e-11	HEAT repeats
PC_rep	PF01851	662 - 695	2.5e-06	Proteasome/cyclosome repeat
RPN2_C	PF18004	797 - 887	5.7e-22	26S proteasome regulatory subunit RPN2 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003773)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04810 AT2G32730
fragaria_vesca	FvH4_4g00680
malus_domestica	MD04G1006300.v1.1 MD08G1218500.v1.1 MD08G1218600.v1.1 MD15G1407100.v1.1
prunus_persica	Prupe.1G006500_v2.0.a1 Prupe.1G006500_v2.0.a1 Prupe.1G554900_v2.0.a1
pyrus_communis	pycom04g00570 pycom08g18930 pycom15g36330
rosa_chinensis	RchiOBHm_Chr4g0386391 RchiOBHm_Chr6g0269091 RchiOBHm_Chr6g0269101 RchiOBHm_Chr6g0269111
rosa_laevigata	RLG00000010168 RLG00000013884
rosa_multiflora	Rmu_co8360047.1_g000001 Rmu_sc0000472.1_g000007 Rmu_sc0002413.1_g000010
rosa_roxburghii	Rroxscaffold_5G00333870
rosa_rugosa	Rorug03G0197200 Rorug03G0306500 Rorug06G0044800
rosa_samantha	Rh3BG283800 Rh4AG010900 Rh4BG008300 Rh4DG009100 Rh6CG164400 Rh6CG164500 Rh6DG156000
rosa_wichuraiana	Rw4G000090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	14
AatII	GACGTC	1 cut(s)	1025
Acc36I	ACCTGC	2 cut(s)	14, 2665
AccB1I	GGYRCC	2 cut(s)	1300, 2090
AccB7I	CCANNNNNTGG	1 cut(s)	1224
AccI	GTMKAC	2 cut(s)	31, 1541
AccIII	TCCGGA	2 cut(s)	58, 1013
AciI	CCGC	5 cut(s)	1247, 1806, 2164, 2253, 2739
AclWI	GGATC	5 cut(s)	264, 277, 386, 399, 953
AcoI	YGGCCR	1 cut(s)	2505
AcsI	RAATTY	4 cut(s)	1005, 1232, 2004, 2238
AcuI	CTGAAG	5 cut(s)	396, 975, 1103, 2046, 2613
AcyI	GRCGYC	3 cut(s)	1022, 1931, 2247
AfaI	GTAC	7 cut(s)	1052, 1346, 1438, 1913, 2020, 2345, 2716
AfiI	CCNNNNNNNGG	6 cut(s)	1224, 1319, 1399, 1806, 2256, 2559
AflII	CTTAAG	1 cut(s)	347
AflIII	ACRYGT	2 cut(s)	282, 1972
AgsI	TTSAA	9 cut(s)	210, 479, 533, 761, 914, 1457, 1736, 2468, 2670
AhdI	GACNNNNNGTC	1 cut(s)	247
AjiI	CACGTC	2 cut(s)	283, 596
AjnI	CCWGG	3 cut(s)	438, 901, 2611
AleI	CACNNNNGTG	1 cut(s)	2309
Alw21I	GWGCWC	3 cut(s)	20, 792, 1363
Alw26I	GTCTC	7 cut(s)	565, 674, 979, 1013, 1640, 1948, 2615
AlwI	GGATC	5 cut(s)	264, 277, 386, 399, 953
AlwNI	CAGNNNCTG	2 cut(s)	1840, 2440
Ama87I	CYCGRG	1 cut(s)	116
Aor13HI	TCCGGA	2 cut(s)	58, 1013
ApoI	RAATTY	4 cut(s)	1005, 1232, 2004, 2238
AseI	ATTAAT	1 cut(s)	576
Asp700I	GAANNNNTTC	1 cut(s)	2644
AspA2I	CCTAGG	1 cut(s)	395
AspLEI	GCGC	2 cut(s)	726, 2085
AspS9I	GGNCC	3 cut(s)	114, 2119, 2562
AsuHPI	GGTGA	3 cut(s)	242, 1574, 2376
AvaI	CYCGRG	1 cut(s)	116
AvaII	GGWCC	1 cut(s)	2119
AvrII	CCTAGG	1 cut(s)	395
AxyI	CCTNAGG	1 cut(s)	1314
BaeGI	GKGCMC	1 cut(s)	2095
BamHI	GGATCC	2 cut(s)	269, 391
BanI	GGYRCC	2 cut(s)	1300, 2090
BanII	GRGCYC	1 cut(s)	20
BarI	GAAGNNNNNNTAC	2 cut(s)	919, 951
BauI	CACGAG	1 cut(s)	2145
BbsI	GAAGAC	3 cut(s)	493, 602, 1091
Bbv12I	GWGCWC	3 cut(s)	20, 792, 1363
BccI	CCATC	6 cut(s)	98, 146, 437, 917, 1291, 2654
BceAI	ACGGC	2 cut(s)	1538, 2492
BcgI	CGANNNNNNTGC	2 cut(s)	1916, 1950
BciT130I	CCWGG	3 cut(s)	440, 903, 2613
BciVI	GTATCC	4 cut(s)	446, 1171, 2194, 2361
BcoDI	GTCTC	7 cut(s)	565, 674, 979, 1013, 1640, 1948, 2615
BfaI	CTAG	7 cut(s)	396, 1253, 1620, 1892, 2136, 2559, 2649
BfmI	CTRYAG	4 cut(s)	327, 1801, 2307, 2577
BfrI	CTTAAG	1 cut(s)	347
BfuAI	ACCTGC	2 cut(s)	14, 2665
BfuI	GTATCC	4 cut(s)	446, 1171, 2194, 2361
BglII	AGATCT	2 cut(s)	120, 1289
BlnI	CCTAGG	1 cut(s)	395
BmcAI	AGTACT	1 cut(s)	1438
Bme1390I	CCNGG	3 cut(s)	440, 903, 2613
Bme18I	GGWCC	1 cut(s)	2119
BmeRI	GACNNNNNGTC	1 cut(s)	247
BmeT110I	CYCGRG	1 cut(s)	116
BmgBI	CACGTC	2 cut(s)	283, 596
BmgT120I	GGNCC	3 cut(s)	114, 2119, 2562
BmiI	GGNNCC	5 cut(s)	271, 393, 538, 1302, 2092
BmrFI	CCNGG	3 cut(s)	440, 903, 2613
BmsI	GCATC	4 cut(s)	187, 561, 733, 1413
BpiI	GAAGAC	3 cut(s)	493, 602, 1091
BpmI	CTGGAG	2 cut(s)	627, 885
BpuEI	CTTGAG	4 cut(s)	521, 673, 2047, 2389
BsaHI	GRCGYC	3 cut(s)	1022, 1931, 2247
BsaJI	CCNNGG	7 cut(s)	259, 395, 902, 1393, 1897, 2086, 2612
BsaWI	WCCGGW	2 cut(s)	58, 1013
Bsc4I	CCNNNNNNNGG	6 cut(s)	1224, 1319, 1399, 1806, 2256, 2559
Bse118I	RCCGGY	1 cut(s)	2249
Bse1I	ACTGG	9 cut(s)	195, 342, 495, 854, 870, 939, 1208, 2026, 2351
Bse21I	CCTNAGG	1 cut(s)	1314
Bse3DI	GCAATG	3 cut(s)	197, 2097, 2109
BseAI	TCCGGA	2 cut(s)	58, 1013
BseBI	CCWGG	3 cut(s)	440, 903, 2613
BseDI	CCNNGG	7 cut(s)	259, 395, 902, 1393, 1897, 2086, 2612
BseGI	GGATG	3 cut(s)	448, 688, 913
BseLI	CCNNNNNNNGG	6 cut(s)	1224, 1319, 1399, 1806, 2256, 2559
BseMI	GCAATG	3 cut(s)	197, 2097, 2109
BseMII	CTCAG	5 cut(s)	861, 1178, 1328, 1908, 1947
BseNI	ACTGG	9 cut(s)	195, 342, 495, 854, 870, 939, 1208, 2026, 2351
BseSI	GKGCMC	1 cut(s)	2095
BseX3I	CGGCCG	1 cut(s)	2505
BsgI	GTGCAG	1 cut(s)	864
Bsh1285I	CGRYCG	1 cut(s)	2508
BshNI	GGYRCC	2 cut(s)	1300, 2090
BsiEI	CGRYCG	1 cut(s)	2508
BsiHKAI	GWGCWC	3 cut(s)	20, 792, 1363
BsiHKCI	CYCGRG	1 cut(s)	116
BsiSI	CCGG	3 cut(s)	59, 1014, 2250
BslFI	GGGAC	4 cut(s)	37, 119, 1389, 1822
BslI	CCNNNNNNNGG	6 cut(s)	1224, 1319, 1399, 1806, 2256, 2559
BsmAI	GTCTC	7 cut(s)	565, 674, 979, 1013, 1640, 1948, 2615
BsmBI	CGTCTC	1 cut(s)	1013
BsmFI	GGGAC	4 cut(s)	37, 119, 1389, 1822
BsmI	GAATGC	2 cut(s)	426, 2378
BsoBI	CYCGRG	1 cut(s)	116
Bsp1286I	GDGCHC	4 cut(s)	20, 792, 1363, 2095
Bsp13I	TCCGGA	2 cut(s)	58, 1013
Bsp1407I	TGTACA	1 cut(s)	1050
Bsp143I	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
Bsp19I	CCATGG	1 cut(s)	1393
BspACI	CCGC	5 cut(s)	1247, 1806, 2164, 2253, 2739
BspCNI	CTCAG	5 cut(s)	860, 1179, 1327, 1909, 1948
BspEI	TCCGGA	2 cut(s)	58, 1013
BspLI	GGNNCC	5 cut(s)	271, 393, 538, 1302, 2092
BspMAI	CTGCAG	2 cut(s)	2311, 2581
BspMI	ACCTGC	2 cut(s)	14, 2665
BspPI	GGATC	5 cut(s)	264, 277, 386, 399, 953
BspT107I	GGYRCC	2 cut(s)	1300, 2090
BspTI	CTTAAG	1 cut(s)	347
BsrDI	GCAATG	3 cut(s)	197, 2097, 2109
BsrFI	RCCGGY	1 cut(s)	2249
BsrGI	TGTACA	1 cut(s)	1050
BsrI	ACTGG	9 cut(s)	195, 342, 495, 854, 870, 939, 1208, 2026, 2351
BssAI	RCCGGY	1 cut(s)	2249
BssECI	CCNNGG	7 cut(s)	259, 395, 902, 1393, 1897, 2086, 2612
BssMI	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
BssNAI	GTATAC	1 cut(s)	1542
BssNI	GRCGYC	3 cut(s)	1022, 1931, 2247
BssSI	CACGAG	1 cut(s)	2145
BssT1I	CCWWGG	5 cut(s)	259, 395, 1393, 1897, 2086
Bst1107I	GTATAC	1 cut(s)	1542
Bst2BI	CACGAG	1 cut(s)	2145
Bst2UI	CCWGG	3 cut(s)	440, 903, 2613
Bst4CI	ACNGT	7 cut(s)	249, 949, 1174, 1413, 1550, 1699, 2488
Bst6I	CTCTTC	5 cut(s)	159, 318, 1503, 1702, 1707
BstACI	GRCGYC	3 cut(s)	1022, 1931, 2247
BstAFI	CTTAAG	1 cut(s)	347
BstAUI	TGTACA	1 cut(s)	1050
BstC8I	GCNNGC	5 cut(s)	1161, 1468, 1632, 1835, 2251
BstDEI	CTNAG	9 cut(s)	10, 354, 581, 847, 1187, 1314, 1917, 1956, 2283
BstDSI	CCRYGG	1 cut(s)	1393
BstF5I	GGATG	3 cut(s)	448, 688, 913
BstHHI	GCGC	2 cut(s)	726, 2085
BstKTI	GATC	9 cut(s)	123, 272, 394, 961, 1072, 1292, 1665, 1753, 2584
BstMAI	GTCTC	7 cut(s)	565, 674, 979, 1013, 1640, 1948, 2615
BstMBI	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
BstMCI	CGRYCG	1 cut(s)	2508
BstNI	CCWGG	3 cut(s)	440, 903, 2613
BstNSI	RCATGY	1 cut(s)	1976
BstSCI	CCNGG	3 cut(s)	438, 901, 2611
BstSFI	CTRYAG	4 cut(s)	327, 1801, 2307, 2577
BstSLI	GKGCMC	1 cut(s)	2095
BstV2I	GAAGAC	3 cut(s)	493, 602, 1091
BstX2I	RGATCY	5 cut(s)	120, 269, 391, 958, 1289
BstYI	RGATCY	5 cut(s)	120, 269, 391, 958, 1289
BstZ17I	GTATAC	1 cut(s)	1542
BstZI	CGGCCG	1 cut(s)	2505
Bsu36I	CCTNAGG	1 cut(s)	1314
BsuI	GTATCC	4 cut(s)	446, 1171, 2194, 2361
BtgI	CCRYGG	1 cut(s)	1393
BtrI	CACGTC	2 cut(s)	283, 596
BtsCI	GGATG	3 cut(s)	448, 688, 913
BtsI	GCAGTG	3 cut(s)	2304, 2316, 2520
BveI	ACCTGC	2 cut(s)	14, 2665
Cac8I	GCNNGC	5 cut(s)	1161, 1468, 1632, 1835, 2251
CaiI	CAGNNNCTG	2 cut(s)	1840, 2440
CfoI	GCGC	2 cut(s)	726, 2085
Cfr10I	RCCGGY	1 cut(s)	2249
Cfr13I	GGNCC	3 cut(s)	114, 2119, 2562
CseI	GACGC	3 cut(s)	121, 1939, 2255
Csp6I	GTAC	7 cut(s)	1051, 1345, 1437, 1912, 2019, 2344, 2715
CviQI	GTAC	7 cut(s)	1051, 1345, 1437, 1912, 2019, 2344, 2715
DdeI	CTNAG	9 cut(s)	10, 354, 581, 847, 1187, 1314, 1917, 1956, 2283
DpnI	GATC	9 cut(s)	122, 271, 393, 960, 1071, 1291, 1664, 1752, 2583
DpnII	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
DriI	GACNNNNNGTC	1 cut(s)	247
EaeI	YGGCCR	1 cut(s)	2505
EagI	CGGCCG	1 cut(s)	2505
Eam1104I	CTCTTC	5 cut(s)	159, 318, 1503, 1702, 1707
Eam1105I	GACNNNNNGTC	1 cut(s)	247
EarI	CTCTTC	5 cut(s)	159, 318, 1503, 1702, 1707
EciI	GGCGGA	1 cut(s)	2268
Ecl136II	GAGCTC	1 cut(s)	18
EclXI	CGGCCG	1 cut(s)	2505
Eco130I	CCWWGG	5 cut(s)	259, 395, 1393, 1897, 2086
Eco24I	GRGCYC	1 cut(s)	20
Eco47I	GGWCC	1 cut(s)	2119
Eco52I	CGGCCG	1 cut(s)	2505
Eco53kI	GAGCTC	1 cut(s)	18
Eco57I	CTGAAG	5 cut(s)	396, 975, 1103, 2046, 2613
Eco81I	CCTNAGG	1 cut(s)	1314
Eco88I	CYCGRG	1 cut(s)	116
EcoICRI	GAGCTC	1 cut(s)	18
EcoRI	GAATTC	1 cut(s)	2238
EcoRII	CCWGG	3 cut(s)	438, 901, 2611
EcoT14I	CCWWGG	5 cut(s)	259, 395, 1393, 1897, 2086
EcoT22I	ATGCAT	1 cut(s)	1789
EcoT38I	GRGCYC	1 cut(s)	20
ErhI	CCWWGG	5 cut(s)	259, 395, 1393, 1897, 2086
Esp3I	CGTCTC	1 cut(s)	1013
FalI	AAGNNNNNCTT	4 cut(s)	732, 764, 1022, 1054
FaqI	GGGAC	4 cut(s)	37, 119, 1389, 1822
FauI	CCCGC	2 cut(s)	1240, 1799
FauNDI	CATATG	2 cut(s)	1636, 2446
FblI	GTMKAC	2 cut(s)	31, 1541
FokI	GGATG	3 cut(s)	455, 695, 920
FriOI	GRGCYC	1 cut(s)	20
FspBI	CTAG	7 cut(s)	396, 1253, 1620, 1892, 2136, 2559, 2649
GlaI	GCGC	2 cut(s)	725, 2084
GsuI	CTGGAG	2 cut(s)	627, 885
HapII	CCGG	3 cut(s)	59, 1014, 2250
HgaI	GACGC	3 cut(s)	121, 1939, 2255
HhaI	GCGC	2 cut(s)	726, 2085
Hin1I	GRCGYC	3 cut(s)	1022, 1931, 2247
Hin6I	GCGC	2 cut(s)	724, 2083
HinP1I	GCGC	2 cut(s)	724, 2083
HincII	GTYRAC	2 cut(s)	1035, 2535
HindII	GTYRAC	2 cut(s)	1035, 2535
HindIII	AAGCTT	1 cut(s)	795
HpaII	CCGG	3 cut(s)	59, 1014, 2250
HphI	GGTGA	3 cut(s)	242, 1574, 2376
Hpy166II	GTNNAC	7 cut(s)	32, 233, 1035, 1542, 1589, 2535, 2680
Hpy8I	GTNNAC	7 cut(s)	32, 233, 1035, 1542, 1589, 2535, 2680
Hpy99I	CGWCG	1 cut(s)	1027
HpyCH4III	ACNGT	7 cut(s)	249, 949, 1174, 1413, 1550, 1699, 2488
HpyCH4IV	ACGT	3 cut(s)	282, 595, 1022
HpyF3I	CTNAG	9 cut(s)	10, 354, 581, 847, 1187, 1314, 1917, 1956, 2283
HpySE526I	ACGT	3 cut(s)	282, 595, 1022
Hsp92I	GRCGYC	3 cut(s)	1022, 1931, 2247
HspAI	GCGC	2 cut(s)	724, 2083
Kpn2I	TCCGGA	2 cut(s)	58, 1013
KroI	GCCGGC	1 cut(s)	2249
KroNI	GCCGGC	1 cut(s)	2251
Kzo9I	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
LmnI	GCTCC	7 cut(s)	61, 263, 723, 835, 904, 2153, 2219
LweI	GCATC	4 cut(s)	187, 561, 733, 1413
MaeI	CTAG	7 cut(s)	396, 1253, 1620, 1892, 2136, 2559, 2649
MaeII	ACGT	3 cut(s)	282, 595, 1022
MaeIII	GTNAC	3 cut(s)	943, 2263, 2302
MalI	GATC	9 cut(s)	122, 271, 393, 960, 1071, 1291, 1664, 1752, 2583
MboI	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
MfeI	CAATTG	2 cut(s)	474, 2168
MflI	RGATCY	5 cut(s)	120, 269, 391, 958, 1289
MhlI	GDGCHC	4 cut(s)	20, 792, 1363, 2095
MlyI	GAGTC	4 cut(s)	59, 236, 1371, 1967
MmeI	TCCRAC	4 cut(s)	108, 381, 1579, 2131
Mph1103I	ATGCAT	1 cut(s)	1789
MreI	CGCCGGCG	1 cut(s)	2249
MroI	TCCGGA	2 cut(s)	58, 1013
MroNI	GCCGGC	1 cut(s)	2249
MroXI	GAANNNNTTC	1 cut(s)	2644
MseI	TTAA	6 cut(s)	237, 348, 576, 806, 1208, 1527
MslI	CAYNNNNRTG	2 cut(s)	2102, 2309
MspA1I	CMGCKG	3 cut(s)	1247, 1571, 1837
MspCI	CTTAAG	1 cut(s)	347
MspI	CCGG	3 cut(s)	59, 1014, 2250
MspR9I	CCNGG	3 cut(s)	440, 903, 2613
MunI	CAATTG	2 cut(s)	474, 2168
Mva1269I	GAATGC	2 cut(s)	426, 2378
MvaI	CCWGG	3 cut(s)	440, 903, 2613
NaeI	GCCGGC	1 cut(s)	2251
NcoI	CCATGG	1 cut(s)	1393
NdeI	CATATG	2 cut(s)	1636, 2446
NdeII	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
NgoMIV	GCCGGC	1 cut(s)	2249
NlaIV	GGNNCC	5 cut(s)	271, 393, 538, 1302, 2092
NmuCI	GTSAC	3 cut(s)	943, 2263, 2302
NsiI	ATGCAT	1 cut(s)	1789
NspI	RCATGY	1 cut(s)	1976
OliI	CACNNNNGTG	1 cut(s)	2309
PaqCI	CACCTGC	1 cut(s)	14
PciI	ACATGT	1 cut(s)	1972
PctI	GAATGC	2 cut(s)	426, 2378
PdiI	GCCGGC	1 cut(s)	2251
PdmI	GAANNNNTTC	1 cut(s)	2644
PfeI	GAWTC	6 cut(s)	293, 365, 866, 1195, 2141, 2666
PflMI	CCANNNNNTGG	1 cut(s)	1224
PfoI	TCCNGGA	1 cut(s)	438
PleI	GAGTC	4 cut(s)	58, 236, 1371, 1966
PpsI	GAGTC	4 cut(s)	58, 236, 1371, 1966
PscI	ACATGT	1 cut(s)	1972
PshBI	ATTAAT	1 cut(s)	576
Psp124BI	GAGCTC	1 cut(s)	20
Psp6I	CCWGG	3 cut(s)	438, 901, 2611
PspGI	CCWGG	3 cut(s)	438, 901, 2611
PspN4I	GGNNCC	5 cut(s)	271, 393, 538, 1302, 2092
PspPI	GGNCC	3 cut(s)	114, 2119, 2562
PstI	CTGCAG	2 cut(s)	2311, 2581
PstNI	CAGNNNCTG	2 cut(s)	1840, 2440
PsuI	RGATCY	5 cut(s)	120, 269, 391, 958, 1289
PvuII	CAGCTG	2 cut(s)	1571, 1837
RsaI	GTAC	7 cut(s)	1052, 1346, 1438, 1913, 2020, 2345, 2716
RsaNI	GTAC	7 cut(s)	1051, 1345, 1437, 1912, 2019, 2344, 2715
RseI	CAYNNNNRTG	2 cut(s)	2102, 2309
SacI	GAGCTC	1 cut(s)	20
SaqAI	TTAA	6 cut(s)	237, 348, 576, 806, 1208, 1527
Sau3AI	GATC	9 cut(s)	120, 269, 391, 958, 1069, 1289, 1662, 1750, 2581
Sau96I	GGNCC	3 cut(s)	114, 2119, 2562
ScaI	AGTACT	1 cut(s)	1438
SchI	GAGTC	4 cut(s)	59, 236, 1371, 1967
ScrFI	CCNGG	3 cut(s)	440, 903, 2613
SduI	GDGCHC	4 cut(s)	20, 792, 1363, 2095
SfaNI	GCATC	4 cut(s)	187, 561, 733, 1413
SfcI	CTRYAG	4 cut(s)	327, 1801, 2307, 2577
SgrAI	CRCCGGYG	1 cut(s)	2249
SinI	GGWCC	1 cut(s)	2119
SmiMI	CAYNNNNRTG	2 cut(s)	2102, 2309
SmlI	CTYRAG	5 cut(s)	347, 500, 652, 2026, 2368
SmoI	CTYRAG	5 cut(s)	347, 500, 652, 2026, 2368
SsiI	CCGC	5 cut(s)	1247, 1806, 2164, 2253, 2739
SspMI	CTAG	7 cut(s)	396, 1253, 1620, 1892, 2136, 2559, 2649
SstI	GAGCTC	1 cut(s)	20
StyD4I	CCNGG	3 cut(s)	438, 901, 2611
StyI	CCWWGG	5 cut(s)	259, 395, 1393, 1897, 2086
TaaI	ACNGT	7 cut(s)	249, 949, 1174, 1413, 1550, 1699, 2488
TaiI	ACGT	3 cut(s)	285, 598, 1025
TaqI	TCGA	3 cut(s)	484, 1025, 1978
TatI	WGTACW	4 cut(s)	1050, 1436, 1911, 2714
TauI	GCSGC	1 cut(s)	2167
TfiI	GAWTC	6 cut(s)	293, 365, 866, 1195, 2141, 2666
Tru1I	TTAA	6 cut(s)	237, 348, 576, 806, 1208, 1527
Tru9I	TTAA	6 cut(s)	237, 348, 576, 806, 1208, 1527
TseFI	GTSAC	3 cut(s)	943, 2263, 2302
Tsp45I	GTSAC	3 cut(s)	943, 2263, 2302
TspDTI	ATGAA	9 cut(s)	171, 177, 296, 558, 699, 903, 1373, 1521, 1671
TspGWI	ACGGA	1 cut(s)	1332
Van91I	CCANNNNNTGG	1 cut(s)	1224
Vha464I	CTTAAG	1 cut(s)	347
VpaK11BI	GGWCC	1 cut(s)	2119
VspI	ATTAAT	1 cut(s)	576
XapI	RAATTY	4 cut(s)	1005, 1232, 2004, 2238
XceI	RCATGY	1 cut(s)	1976
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2212
XmaJI	CCTAGG	1 cut(s)	395
XmiI	GTMKAC	2 cut(s)	31, 1541
XmnI	GAANNNNTTC	1 cut(s)	2644
XspI	CTAG	7 cut(s)	396, 1253, 1620, 1892, 2136, 2559, 2649
ZraI	GACGTC	1 cut(s)	1023
ZrmI	AGTACT	1 cut(s)	1438
Zsp2I	ATGCAT	1 cut(s)	1789

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.