Rh3DG172600

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006969
Physical Location & Seq
Reverse (-)
15174003 .. 15176373
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG172600.1

Sequence Viewer

Length: 867 bp
ATGGCCCGCAGAATTGGAGGCATTTTGGGAGTGTTTGTTGGATTGGATCCACATAAGAAAAGTGATTGCACGGGAAGTGTTCTTCTCATACGAGTTGGGTTGGATGTCTCCCAGCCACTACGGCGATGGATCAAATTGGATTTGGGGTTGGGGGAGGAATCCAAGTTGCATCTAGAGTATGAAGATCTACCCTATTTCTGCTTCTACTGTGGACGTCTTTCCCATGTGAGCTCTCACTGTCCATGGTTTAAGGATGGGACGATCACTGAAATGCAGTATGGTCGGTGGAAAACTTTATTGAAGAATGTGTTCACCATTGACCCAGAGTGCCAGTTGAAGGGAGCGTCTTTTGGTCTCATCAAGGACAAGTTGCAGTGGAAGATGACAGCTCCTGAGCCTATGTTGAGTGGAGCAGTGCGTTCCCGGGCAGAATGGGTTGAAGAGAATCCGATCCAGGAGGTTAGAATGGATGTAGATGGAGTTGAAGAGATGGTGGAGAAACAGCCGGAGGCAAAACGGCGGCGCGTGATTCCCTTAGCCAAAGCGCGTGAAGATGGAGTGTTAATTATGGGGAGTGATTCTAGGGATGCTGCTCTGATTTCTCTCCCTTTGATTTCGGAGACGGTTTTGCCAGAGTTTATGTCTGGACCAATGGTTGGTATTAAAGCGGGAATAGAGAGGAATTTGGGTTTGGATACGGTTTCTCACGAAGTGGGTGGGCCGGGTGAGAATGGGCCTACTGGTTCAGTTGCTAAGTCCAGAGGGAGGCCCAGAAGTGTATCAGTTGATTATAAACTCCTTAATAATGGGAGTGTGGGCAAGAGTACTTCTAGAAATTTCCAGAAGGAGTTGATTTCCGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

288

Amino Acids

31.92

Weight (kDa)

6.97

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 34 - 81 9.1e-12 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AatII GACGTC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 656
AccII CGCG 2 cut(s) 525, 547
AciI CCGC 3 cut(s) 7, 520, 668
AclWI GGATC 4 cut(s) 41, 54, 137, 445
AcsI RAATTY 2 cut(s) 682, 835
AcyI GRCGYC 1 cut(s) 214
AdeI CACNNNGTG 1 cut(s) 712
AfaI GTAC 1 cut(s) 826
AfiI CCNNNNNNNGG 3 cut(s) 337, 656, 765
AgsI TTSAA 4 cut(s) 301, 337, 440, 485
AjnI CCWGG 1 cut(s) 453
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
AluBI AGCT 2 cut(s) 231, 389
AluI AGCT 2 cut(s) 231, 389
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 3 cut(s) 112, 359, 614
AlwI GGATC 4 cut(s) 41, 54, 137, 445
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 4 cut(s) 3, 719, 734, 767
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 2 cut(s) 682, 835
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 2 cut(s) 525, 547
AspS9I GGNCC 5 cut(s) 4, 647, 719, 734, 768
AsuC2I CCSGG 3 cut(s) 424, 425, 723
AsuHPI GGTGA 2 cut(s) 304, 737
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 1 cut(s) 647
BamHI GGATCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 577
BccI CCATC 5 cut(s) 120, 248, 470, 484, 548
BceAI ACGGC 2 cut(s) 137, 533
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 455
BciVI GTATCC 1 cut(s) 688
BcnI CCSGG 3 cut(s) 424, 425, 723
BcoDI GTCTC 3 cut(s) 112, 359, 614
BfaI CTAG 3 cut(s) 173, 582, 831
BfuI GTATCC 1 cut(s) 688
BglI GCCNNNNNGGC 1 cut(s) 121
BglII AGATCT 1 cut(s) 184
BisI GCNGC 2 cut(s) 521, 591
BlsI GCNGC 2 cut(s) 522, 592
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 4 cut(s) 424, 425, 455, 723
Bme18I GGWCC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 5 cut(s) 4, 647, 719, 734, 768
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 4 cut(s) 424, 425, 455, 723
BmsI GCATC 2 cut(s) 178, 577
Bpu10I CCTNAGC 2 cut(s) 393, 535
BpuMI CCSGG 3 cut(s) 424, 425, 723
BsaHI GRCGYC 1 cut(s) 214
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 2 cut(s) 242, 423
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 656, 765
Bse1I ACTGG 2 cut(s) 331, 745
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 2 cut(s) 242, 423
BseGI GGATG 4 cut(s) 109, 259, 475, 592
BseLI CCNNNNNNNGG 3 cut(s) 337, 656, 765
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 331, 745
BseXI GCAGC 1 cut(s) 577
BseYI CCCAGC 1 cut(s) 111
Bsh1236I CGCG 2 cut(s) 525, 547
BshFI GGCC 4 cut(s) 5, 721, 736, 769
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 424, 506, 722
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 3 cut(s) 337, 656, 765
BsmAI GTCTC 3 cut(s) 112, 359, 614
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 1 cut(s) 271
BsnI GGCC 4 cut(s) 5, 721, 736, 769
Bso31I GGTCTC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 5 cut(s) 46, 129, 184, 261, 450
Bsp19I CCATGG 1 cut(s) 242
BspACI CCGC 3 cut(s) 7, 520, 668
BspANI GGCC 4 cut(s) 5, 721, 736, 769
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 2 cut(s) 525, 547
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 4 cut(s) 41, 54, 137, 445
BspTNI GGTCTC 1 cut(s) 359
BsrI ACTGG 2 cut(s) 331, 745
BssECI CCNNGG 2 cut(s) 242, 423
BssMI GATC 5 cut(s) 46, 129, 184, 261, 450
BssNI GRCGYC 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 4 cut(s) 209, 239, 625, 700
Bst6I CTCTTC 2 cut(s) 435, 480
BstACI GRCGYC 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 3 cut(s) 393, 535, 753
BstDSI CCRYGG 1 cut(s) 242
BstF5I GGATG 4 cut(s) 109, 259, 475, 592
BstFNI CGCG 2 cut(s) 525, 547
BstHHI GCGC 2 cut(s) 525, 547
BstKTI GATC 5 cut(s) 49, 132, 187, 264, 453
BstMAI GTCTC 3 cut(s) 112, 359, 614
BstMBI GATC 5 cut(s) 46, 129, 184, 261, 450
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNI CCWGG 1 cut(s) 455
BstSCI CCNGG 4 cut(s) 422, 423, 453, 721
BstUI CGCG 2 cut(s) 525, 547
BstV1I GCAGC 1 cut(s) 577
BstX2I RGATCY 2 cut(s) 46, 184
BstYI RGATCY 2 cut(s) 46, 184
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 4 cut(s) 5, 721, 736, 769
BtgI CCRYGG 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 4 cut(s) 109, 259, 475, 592
BtsI GCAGTG 2 cut(s) 380, 420
BtsIMutI CAGTG 4 cut(s) 235, 264, 380, 420
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 2 cut(s) 525, 547
Cfr13I GGNCC 5 cut(s) 4, 647, 719, 734, 768
Cfr9I CCCGGG 1 cut(s) 423
CseI GACGC 1 cut(s) 333
Csp6I GTAC 1 cut(s) 825
CviAII CATG 2 cut(s) 224, 243
CviQI GTAC 1 cut(s) 825
DdeI CTNAG 3 cut(s) 393, 535, 753
DpnI GATC 5 cut(s) 48, 131, 186, 263, 452
DpnII GATC 5 cut(s) 46, 129, 184, 261, 450
DraIII CACNNNGTG 1 cut(s) 712
Eam1104I CTCTTC 2 cut(s) 435, 480
EarI CTCTTC 2 cut(s) 435, 480
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 242
Esp3I CGTCTC 1 cut(s) 614
FaeI CATG 2 cut(s) 227, 246
FaqI GGGAC 1 cut(s) 271
FatI CATG 2 cut(s) 223, 242
FauI CCCGC 2 cut(s) 14, 661
Fnu4HI GCNGC 2 cut(s) 521, 591
FokI GGATG 4 cut(s) 116, 266, 482, 599
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 2 cut(s) 521, 591
FspBI CTAG 3 cut(s) 173, 582, 831
GlaI GCGC 2 cut(s) 524, 546
GluI GCNGC 2 cut(s) 521, 591
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 4 cut(s) 5, 721, 736, 769
HapII CCGG 3 cut(s) 424, 506, 722
HgaI GACGC 1 cut(s) 333
HhaI GCGC 2 cut(s) 525, 547
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 2 cut(s) 227, 246
Hin6I GCGC 2 cut(s) 523, 545
HinP1I GCGC 2 cut(s) 523, 545
HinfI GANTC 4 cut(s) 158, 445, 529, 578
HpaII CCGG 3 cut(s) 424, 506, 722
HphI GGTGA 2 cut(s) 304, 737
Hpy166II GTNNAC 2 cut(s) 212, 312
Hpy188I TCNGA 4 cut(s) 450, 597, 619, 859
Hpy188III TCNNGA 7 cut(s) 173, 392, 645, 707, 759, 831, 841
Hpy8I GTNNAC 2 cut(s) 212, 312
HpyAV CCTTC 2 cut(s) 331, 838
HpyCH4III ACNGT 4 cut(s) 209, 239, 625, 700
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 4 cut(s) 69, 169, 274, 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 3 cut(s) 393, 535, 753
HpySE526I ACGT 1 cut(s) 214
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 2 cut(s) 227, 246
HspAI GCGC 2 cut(s) 523, 545
Kzo9I GATC 5 cut(s) 46, 129, 184, 261, 450
LmnI GCTCC 3 cut(s) 341, 394, 410
Lsp1109I GCAGC 1 cut(s) 577
LweI GCATC 2 cut(s) 178, 577
MaeI CTAG 3 cut(s) 173, 582, 831
MaeII ACGT 1 cut(s) 214
MalI GATC 5 cut(s) 48, 131, 186, 263, 452
MboI GATC 5 cut(s) 46, 129, 184, 261, 450
MboII GAAGA 7 cut(s) 74, 194, 313, 391, 452, 497, 563
MflI RGATCY 2 cut(s) 46, 184
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 5 cut(s) 12, 134, 564, 682, 835
MmeI TCCRAC 2 cut(s) 19, 81
MnlI CCTC 7 cut(s) 11, 148, 451, 502, 672, 755, 759
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 4 cut(s) 249, 563, 663, 801
MslI CAYNNNNRTG 1 cut(s) 269
MspI CCGG 3 cut(s) 424, 506, 722
MspR9I CCNGG 4 cut(s) 424, 425, 455, 723
MvaI CCWGG 1 cut(s) 455
MvnI CGCG 2 cut(s) 525, 547
MwoI GCNNNNNNNGC 1 cut(s) 121
NciI CCSGG 3 cut(s) 424, 425, 723
NcoI CCATGG 1 cut(s) 242
NdeII GATC 5 cut(s) 46, 129, 184, 261, 450
NlaIII CATG 2 cut(s) 227, 246
NlaIV GGNNCC 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 4 cut(s) 158, 445, 529, 578
PflMI CCANNNNNTGG 1 cut(s) 656
PfoI TCCNGGA 1 cut(s) 453
PkrI GCNGC 2 cut(s) 522, 592
PsiI TTATAA 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 453
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 5 cut(s) 4, 647, 719, 734, 768
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 46, 184
RsaI GTAC 1 cut(s) 826
RsaNI GTAC 1 cut(s) 825
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 4 cut(s) 249, 563, 663, 801
SatI GCNGC 2 cut(s) 521, 591
Sau3AI GATC 5 cut(s) 46, 129, 184, 261, 450
Sau96I GGNCC 5 cut(s) 4, 647, 719, 734, 768
ScaI AGTACT 1 cut(s) 826
ScrFI CCNGG 4 cut(s) 424, 425, 455, 723
SduI GDGCHC 1 cut(s) 233
SetI ASST 4 cut(s) 217, 233, 391, 462
SfaNI GCATC 2 cut(s) 178, 577
SinI GGWCC 1 cut(s) 647
SmaI CCCGGG 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 269
Sse9I AATT 5 cut(s) 12, 134, 564, 682, 835
SsiI CCGC 3 cut(s) 7, 520, 668
SspMI CTAG 3 cut(s) 173, 582, 831
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 4 cut(s) 422, 423, 453, 721
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 4 cut(s) 209, 239, 625, 700
TaiI ACGT 1 cut(s) 217
TasI AATT 5 cut(s) 12, 134, 564, 682, 835
TatI WGTACW 1 cut(s) 824
TauI GCSGC 1 cut(s) 523
TfiI GAWTC 4 cut(s) 158, 445, 529, 578
Tru1I TTAA 4 cut(s) 249, 563, 663, 801
Tru9I TTAA 4 cut(s) 249, 563, 663, 801
TscAI CASTG 4 cut(s) 242, 271, 380, 420
TseI GCWGC 1 cut(s) 590
TspDTI ATGAA 1 cut(s) 195
TspMI CCCGGG 1 cut(s) 423
TspRI CASTG 4 cut(s) 242, 271, 380, 420
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 2 cut(s) 682, 835
XbaI TCTAGA 2 cut(s) 172, 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
XmaI CCCGGG 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 3 cut(s) 173, 582, 831
ZraI GACGTC 1 cut(s) 215
ZrmI AGTACT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.