Rh5BG530100

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006969
Physical Location & Seq
Reverse (-)
83503540 .. 83504240
701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG530100.1

Sequence Viewer

Length: 636 bp
ATGGATTTAGGGTTTAGTGAGGAAACCAAATTGCAATTGGAATATGAAGAGCTACCCTACTTTTGTTTCTACTGTGGACGCCTCTCACATGTTAGCTCTAATTGTCCTTGGCATAAAGCAGGGAAGATCAAGGAGATGCAGTATGGAAGGTGGAAAACCCTTGCGAAGAATGTGTTTTGCATTGATCCGGACGGACAACTGAAGGGTAGGGCATTTGGGTTCGTCAGGGAGAAACCTCACTGGAAGGTGAAAGATCCGGAGCCGACCCTGATTGGATCTGTTCATTCATGTGATGAGTGGGGTGGAGAGATGGAGATTCAGGAAACTAGAATGGAAGTTGATGGGACGATGGTTACGATGGTTGTGATGCAGCCGGAAGCAAAGCGAAGGCGCTTTGGACACAAAAGTCCAAAGCGCGTGAGGGGGGATGTTTTGCCAGAAGTTATATCCAAGCAAGATGATTGCATGGAGGCGGGAATGGAAGGTAATTCAAATGTTGTTCATGTTTCTCAAAAAGACAATGGGCTGAAGGAGTGCGGTAGTGGACTGTTAGGTTCTAATTGTATATCTAGAGGGTGGCCTAAAGGTGGTAAGGTTGACTACAAACTCCTCAATAGTGGAAGTGCGCGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

211

Amino Acids

23.85

Weight (kDa)

8.37

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 7 - 36 3.6e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 621
AccII CGCG 2 cut(s) 417, 628
AccIII TCCGGA 2 cut(s) 187, 256
AciI CCGC 2 cut(s) 473, 537
AclWI GGATC 3 cut(s) 179, 248, 283
AcuI CTGAAG 2 cut(s) 221, 548
AcyI GRCGYC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 587
AflIII ACRYGT 1 cut(s) 88
AgsI TTSAA 1 cut(s) 492
AluBI AGCT 2 cut(s) 52, 96
AluI AGCT 2 cut(s) 52, 96
AlwI GGATC 3 cut(s) 179, 248, 283
Aor13HI TCCGGA 2 cut(s) 187, 256
AoxI GGCC 1 cut(s) 578
ApeKI GCWGC 1 cut(s) 370
AspLEI GCGC 4 cut(s) 393, 417, 628, 630
AsuHPI GGTGA 1 cut(s) 259
BbvI GCAGC 1 cut(s) 382
BccI CCATC 4 cut(s) 304, 335, 343, 352
BfaI CTAG 2 cut(s) 327, 570
BfoI RGCGCY 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 621
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 261
BmsI GCATC 2 cut(s) 126, 357
BsaHI GRCGYC 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 107
BsaWI WCCGGW 2 cut(s) 187, 256
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse1I ACTGG 1 cut(s) 245
BseAI TCCGGA 2 cut(s) 187, 256
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 587
BseNI ACTGG 1 cut(s) 245
BsePI GCGCGC 1 cut(s) 626
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 1 cut(s) 382
Bsh1236I CGCG 2 cut(s) 417, 628
BshFI GGCC 1 cut(s) 580
BsiSI CCGG 3 cut(s) 188, 257, 374
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 587
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 1 cut(s) 580
Bsp13I TCCGGA 2 cut(s) 187, 256
Bsp143I GATC 4 cut(s) 126, 184, 253, 275
BspACI CCGC 2 cut(s) 473, 537
BspANI GGCC 1 cut(s) 580
BspEI TCCGGA 2 cut(s) 187, 256
BspFNI CGCG 2 cut(s) 417, 628
BspLI GGNNCC 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 3 cut(s) 179, 248, 283
BspQI GCTCTTC 1 cut(s) 42
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 1 cut(s) 107
BssHII GCGCGC 1 cut(s) 626
BssMI GATC 4 cut(s) 126, 184, 253, 275
BssNI GRCGYC 1 cut(s) 79
BssT1I CCWWGG 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 74, 549
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 628
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 2 cut(s) 417, 628
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 4 cut(s) 393, 417, 628, 630
BstKTI GATC 4 cut(s) 129, 187, 256, 278
BstMBI GATC 4 cut(s) 126, 184, 253, 275
BstNSI RCATGY 1 cut(s) 92
BstUI CGCG 2 cut(s) 417, 628
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 2 cut(s) 253, 275
BstYI RGATCY 2 cut(s) 253, 275
BsuRI GGCC 1 cut(s) 580
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 1 cut(s) 628
CfoI GCGC 4 cut(s) 393, 417, 628, 630
CseI GACGC 1 cut(s) 87
CviAII CATG 4 cut(s) 89, 288, 466, 503
CviJI RGCY 6 cut(s) 52, 96, 262, 373, 526, 580
CviKI_1 RGCY 6 cut(s) 52, 96, 262, 373, 526, 580
DpnI GATC 4 cut(s) 128, 186, 255, 277
DpnII GATC 4 cut(s) 126, 184, 253, 275
DrdI GACNNNNNNGTC 1 cut(s) 405
DseDI GACNNNNNNGTC 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 107
Eco57I CTGAAG 2 cut(s) 221, 548
EcoT14I CCWWGG 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 4 cut(s) 92, 291, 469, 506
FaiI YATR 9 cut(s) 45, 90, 114, 144, 289, 446, 467, 504, 566
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 88, 287, 465, 502
FauI CCCGC 1 cut(s) 466
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 2 cut(s) 327, 570
GlaI GCGC 4 cut(s) 392, 416, 627, 629
GluI GCNGC 1 cut(s) 371
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 1 cut(s) 580
HapII CCGG 3 cut(s) 188, 257, 374
HgaI GACGC 1 cut(s) 87
HhaI GCGC 4 cut(s) 393, 417, 628, 630
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 4 cut(s) 92, 291, 469, 506
Hin6I GCGC 4 cut(s) 391, 415, 626, 628
HinP1I GCGC 4 cut(s) 391, 415, 626, 628
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 1 cut(s) 316
HpaII CCGG 3 cut(s) 188, 257, 374
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 3 cut(s) 77, 545, 598
Hpy188III TCNNGA 4 cut(s) 188, 257, 320, 570
Hpy8I GTNNAC 3 cut(s) 77, 545, 598
HpyAV CCTTC 6 cut(s) 141, 196, 238, 381, 476, 523
HpyCH4III ACNGT 2 cut(s) 74, 549
HpyCH4V TGCA 5 cut(s) 34, 139, 180, 370, 465
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 4 cut(s) 92, 291, 469, 506
HspAI GCGC 4 cut(s) 391, 415, 626, 628
Kpn2I TCCGGA 2 cut(s) 187, 256
Kzo9I GATC 4 cut(s) 126, 184, 253, 275
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 126, 357
MaeI CTAG 2 cut(s) 327, 570
MaeIII GTNAC 1 cut(s) 352
MalI GATC 4 cut(s) 128, 186, 255, 277
MboI GATC 4 cut(s) 126, 184, 253, 275
MboII GAAGA 3 cut(s) 59, 136, 178
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 2 cut(s) 253, 275
MluCI AATT 5 cut(s) 29, 35, 100, 487, 559
MnlI CCTC 7 cut(s) 13, 92, 246, 414, 463, 566, 620
MroI TCCGGA 2 cut(s) 187, 256
MslI CAYNNNNRTG 1 cut(s) 288
MspI CCGG 3 cut(s) 188, 257, 374
MunI CAATTG 1 cut(s) 35
MvnI CGCG 2 cut(s) 417, 628
NdeII GATC 4 cut(s) 126, 184, 253, 275
NlaIII CATG 4 cut(s) 92, 291, 469, 506
NlaIV GGNNCC 1 cut(s) 261
NspI RCATGY 1 cut(s) 92
PauI GCGCGC 1 cut(s) 626
PciI ACATGT 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 353
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 1 cut(s) 372
PscI ACATGT 1 cut(s) 88
PspN4I GGNNCC 1 cut(s) 261
PsuI RGATCY 2 cut(s) 253, 275
PteI GCGCGC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 42
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 4 cut(s) 126, 184, 253, 275
SfaNI GCATC 2 cut(s) 126, 357
SmiMI CAYNNNNRTG 1 cut(s) 288
Sse9I AATT 5 cut(s) 29, 35, 100, 487, 559
SsiI CCGC 2 cut(s) 473, 537
SspMI CTAG 2 cut(s) 327, 570
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 74, 549
TasI AATT 5 cut(s) 29, 35, 100, 487, 559
TfiI GAWTC 1 cut(s) 316
TscAI CASTG 1 cut(s) 245
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 4 cut(s) 60, 272, 276, 491
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 569
XceI RCATGY 1 cut(s) 92
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XspI CTAG 2 cut(s) 327, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.