Rw6G002310

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006969
Physical Location & Seq
Reverse (-)
3882475 .. 3884052
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002310.1

Sequence Viewer

Length: 1323 bp
ATGAGTACGATATGGGGGCTGGTAAGGGAAGTATCAAAAGGAGTGGTGGGTTTTTGGGAGAAGATGGAAGAGGTGGTGAATGGTCTGTCTGCCAAACTGGTAATTTCAGAGGCGGAGGACCTTAAGGTGGTTAAGATTGATGATGTGGAGGATATGTCTCTCAAGAAGTACTGGTTAGTGAGGAGACTTCTAACTTCGAAACCTTTCAACAAGGAGTCACTTCGGAGTACTATGAAGAGGATCTGGCATATTAAAGGAAAATTGGTGGACGTTGACCCTGGGGTGAATGGAGACTGCTCTACGAGCTTTCTCTTAATCCGAGTGGGGTTGGATGTCAATGTGCCTCTAAGAAGATGGATTAGGCTTCAACTTGAGAATTCAGGAGAGGAAGTCAAACTCTTACTGGATTACGAGAACCTTCCCTACTTTTGTTTATATTGTGGTTGTTTGGATCATGTTAGCTCTTGCTGTGAACTGAAAAAGGCGGGGAAAATGCAGGAAGTGCAGTATGGTAGGTGGAAGACCCTTTCAAAGAATGTGTTTAACATTGATCCTCATGGTCAATTACAAGGTGTCAGATTTGGTCTTCATAGTCCTAAGCCCGGTTGGAGGATGCAGAAACCTTCGTCGTCATTGACTGGGGTGATCAGGCCAAGAGATGAGTTTCTAGGAAGCGGTTGTGGTCTGAATAGTGAAATGGTGGTGGATGGTGAGTTGGCAGGGGATCACCTTGAGGCACTGGAGGCAAAGCAGAGGTGGATAGCGGTCCCTCCGCCTTCTTCAGACGTGCCTGATAATATGGAGGTAATTGAGGAAAGACCAATTGTTACGGGAAAGAATATTGGAGGATCCAATCAGTCATTATTACAAAAGCATTCACCGCAGTCTCAGGCGGGGTTGGAGGATATTCATACCCTAAATCATCACTTGAGATCCGACATCCTCTCAGCTTCGATTTTTGAAGATTTACAGATTGGCGGGACGTTAGGAGGCGTAAGTTTAAGAGCTGCTTCAAATTTGAAGTTGGGCGGTGTTAATGGGCATGATGAGCTGGCAAGTCCTTCAGTAAAACAACAGGAAATTTGGCCCATTTTGAAACTTGAGGGGGTCAATAGGAATCACAGGTTGGTGGAGGACTCTCAGGTTGTGGAGCAAAGTGGGGATGCGGTGGTTTATAAGAGAGTTGGAAAGAAAAAGGGAGAGAAGAAAGAAGCGTTAGGAAAGCAGGCAGCGTCAAAGGGAGAGAATGGAGGTCATGGAGGTCGGGTTGGCTTCTTCGTGGGAGGTCAGGGGAGACGAAGGAGTAAGGCTTCTCCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

440

Amino Acids

48.59

Weight (kDa)

8.67

Isoelectric Point (pI)

54.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 110 - 158 2.2e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1176
AclWI GGATC 7 cut(s) 248, 459, 545, 732, 843, 856, 927
AcsI RAATTY 3 cut(s) 376, 1015, 1080
AcuI CTGAAG 2 cut(s) 765, 1047
AfaI GTAC 3 cut(s) 7, 170, 229
AfiI CCNNNNNNNGG 3 cut(s) 127, 602, 609
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 7 cut(s) 208, 368, 531, 962, 1014, 1021, 1096
AjiI CACGTC 1 cut(s) 787
AjnI CCWGG 1 cut(s) 277
AjuI GAANNNNNNNTTGG 2 cut(s) 1109, 1141
AluBI AGCT 5 cut(s) 306, 462, 950, 1007, 1051
AluI AGCT 5 cut(s) 306, 462, 950, 1007, 1051
Alw26I GTCTC 5 cut(s) 162, 178, 285, 891, 1288
AlwI GGATC 7 cut(s) 248, 459, 545, 732, 843, 856, 927
AoxI GGCC 2 cut(s) 650, 1085
ApeKI GCWGC 2 cut(s) 1007, 1229
ApoI RAATTY 3 cut(s) 376, 1015, 1080
Asp700I GAANNNNTTC 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 118, 766, 1086
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 6 cut(s) 88, 295, 655, 719, 722, 870
AsuII TTCGAA 1 cut(s) 197
AvaII GGWCC 2 cut(s) 118, 766
BamHI GGATCC 1 cut(s) 848
BbsI GAAGAC 2 cut(s) 527, 578
BbvI GCAGC 2 cut(s) 994, 1241
BccI CCATC 3 cut(s) 58, 348, 701
BciT130I CCWGG 1 cut(s) 279
BclI TGATCA 1 cut(s) 645
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 5 cut(s) 162, 178, 285, 891, 1288
BfaI CTAG 1 cut(s) 668
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 2 cut(s) 1008, 1230
BlsI GCNGC 2 cut(s) 1009, 1231
BmcAI AGTACT 2 cut(s) 170, 229
Bme1390I CCNGG 2 cut(s) 279, 603
Bme18I GGWCC 2 cut(s) 118, 766
BmgBI CACGTC 1 cut(s) 787
BmgT120I GGNCC 3 cut(s) 118, 766, 1086
BmiI GGNNCC 2 cut(s) 768, 850
BmrFI CCNGG 2 cut(s) 279, 603
BmrI ACTGGG 1 cut(s) 648
BmsI GCATC 2 cut(s) 603, 1153
BmuI ACTGGG 1 cut(s) 648
BpiI GAAGAC 2 cut(s) 527, 578
BpmI CTGGAG 1 cut(s) 761
Bpu10I CCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 197
BpuEI CTTGAG 5 cut(s) 146, 392, 752, 949, 1121
BpuMI CCSGG 1 cut(s) 603
BsaJI CCNNGG 2 cut(s) 277, 278
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 375, 405, 1251, 1281
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 602, 609
Bse1I ACTGG 5 cut(s) 102, 176, 408, 643, 744
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 277, 278
BseGI GGATG 5 cut(s) 337, 618, 712, 939, 1168
BseLI CCNNNNNNNGG 3 cut(s) 127, 602, 609
BseMII CTCAG 3 cut(s) 902, 960, 1154
BseNI ACTGG 5 cut(s) 102, 176, 408, 643, 744
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 2 cut(s) 994, 1241
BsgI GTGCAG 1 cut(s) 524
BshFI GGCC 2 cut(s) 652, 1087
BsiSI CCGG 1 cut(s) 603
BslFI GGGAC 2 cut(s) 752, 994
BslI CCNNNNNNNGG 3 cut(s) 127, 602, 609
BsmAI GTCTC 5 cut(s) 162, 178, 285, 891, 1288
BsmBI CGTCTC 1 cut(s) 1288
BsmFI GGGAC 2 cut(s) 752, 994
BsmI GAATGC 1 cut(s) 874
BsnI GGCC 2 cut(s) 652, 1087
Bsp119I TTCGAA 1 cut(s) 197
Bsp143I GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
BspANI GGCC 2 cut(s) 652, 1087
BspCNI CTCAG 3 cut(s) 901, 959, 1153
BspLI GGNNCC 2 cut(s) 768, 850
BspPI GGATC 7 cut(s) 248, 459, 545, 732, 843, 856, 927
BspT104I TTCGAA 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 122
BsrI ACTGG 5 cut(s) 102, 176, 408, 643, 744
BssECI CCNNGG 2 cut(s) 277, 278
BssMI GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
Bst2UI CCWGG 1 cut(s) 279
Bst6I CTCTTC 2 cut(s) 63, 230
BstAFI CTTAAG 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 502
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 1053, 1227
BstDEI CTNAG 5 cut(s) 347, 597, 888, 946, 1140
BstF5I GGATG 5 cut(s) 337, 618, 712, 939, 1168
BstKTI GATC 7 cut(s) 243, 454, 553, 648, 727, 851, 935
BstMAI GTCTC 5 cut(s) 162, 178, 285, 891, 1288
BstMBI GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
BstMWI GCNNNNNNNGC 5 cut(s) 303, 502, 743, 880, 1048
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 2 cut(s) 277, 601
BstV1I GCAGC 2 cut(s) 994, 1241
BstV2I GAAGAC 2 cut(s) 527, 578
BstX2I RGATCY 3 cut(s) 240, 848, 932
BstYI RGATCY 3 cut(s) 240, 848, 932
BsuRI GGCC 2 cut(s) 652, 1087
BtrI CACGTC 1 cut(s) 787
BtsCI GGATG 5 cut(s) 337, 618, 712, 939, 1168
BtsIMutI CAGTG 1 cut(s) 737
Cac8I GCNNGC 2 cut(s) 1053, 1227
Cfr13I GGNCC 3 cut(s) 118, 766, 1086
CseI GACGC 1 cut(s) 1221
Csp6I GTAC 3 cut(s) 6, 169, 228
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 4 cut(s) 455, 557, 1043, 1256
CviQI GTAC 3 cut(s) 6, 169, 228
DdeI CTNAG 5 cut(s) 347, 597, 888, 946, 1140
DpnI GATC 7 cut(s) 242, 453, 552, 647, 726, 850, 934
DpnII GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
Eam1104I CTCTTC 2 cut(s) 63, 230
EarI CTCTTC 2 cut(s) 63, 230
EciI GGCGGA 2 cut(s) 128, 762
Eco47I GGWCC 2 cut(s) 118, 766
Eco57I CTGAAG 2 cut(s) 765, 1047
EcoO109I RGGNCCY 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 277
Esp3I CGTCTC 1 cut(s) 1288
FaeI CATG 4 cut(s) 458, 560, 1046, 1259
FalI AAGNNNNNCTT 2 cut(s) 994, 1026
FaqI GGGAC 2 cut(s) 752, 994
FatI CATG 4 cut(s) 454, 556, 1042, 1255
FauI CCCGC 3 cut(s) 478, 886, 971
FbaI TGATCA 1 cut(s) 645
Fnu4HI GCNGC 2 cut(s) 1008, 1230
FokI GGATG 5 cut(s) 344, 625, 719, 926, 1175
Fsp4HI GCNGC 2 cut(s) 1008, 1230
FspBI CTAG 1 cut(s) 668
GluI GCNGC 2 cut(s) 1008, 1230
GsuI CTGGAG 1 cut(s) 761
HaeIII GGCC 2 cut(s) 652, 1087
HapII CCGG 1 cut(s) 603
HgaI GACGC 1 cut(s) 1221
Hin1II CATG 4 cut(s) 458, 560, 1046, 1259
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 3 cut(s) 215, 1117, 1136
HpaII CCGG 1 cut(s) 603
HphI GGTGA 6 cut(s) 88, 295, 655, 719, 722, 870
Hpy166II GTNNAC 3 cut(s) 268, 274, 473
Hpy188I TCNGA 7 cut(s) 109, 225, 320, 578, 687, 784, 937
Hpy188III TCNNGA 3 cut(s) 163, 381, 1316
Hpy8I GTNNAC 3 cut(s) 268, 274, 473
Hpy99I CGWCG 1 cut(s) 631
HpyAV CCTTC 5 cut(s) 428, 633, 786, 1071, 1293
HpyCH4IV ACGT 3 cut(s) 270, 786, 983
HpyCH4V TGCA 3 cut(s) 496, 505, 616
HpyF10VI GCNNNNNNNGC 5 cut(s) 303, 502, 743, 880, 1048
HpyF3I CTNAG 5 cut(s) 347, 597, 888, 946, 1140
HpySE526I ACGT 3 cut(s) 270, 786, 983
Hsp92II CATG 4 cut(s) 458, 560, 1046, 1259
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
LmnI GCTCC 1 cut(s) 1150
Lsp1109I GCAGC 2 cut(s) 994, 1241
LweI GCATC 2 cut(s) 603, 1153
MaeI CTAG 1 cut(s) 668
MaeII ACGT 3 cut(s) 270, 786, 983
MaeIII GTNAC 2 cut(s) 216, 826
MalI GATC 7 cut(s) 242, 453, 552, 647, 726, 850, 934
MboI GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
MfeI CAATTG 1 cut(s) 822
MflI RGATCY 3 cut(s) 240, 848, 932
MluCI AATT 8 cut(s) 102, 260, 376, 563, 807, 822, 1015, 1080
MlyI GAGTC 2 cut(s) 224, 1130
MmeI TCCRAC 5 cut(s) 309, 587, 879, 960, 1165
MroXI GAANNNNTTC 1 cut(s) 203
MseI TTAA 7 cut(s) 123, 132, 252, 314, 543, 1001, 1035
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 2 cut(s) 279, 603
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 1 cut(s) 874
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 5 cut(s) 303, 502, 743, 880, 1048
NciI CCSGG 1 cut(s) 603
NdeII GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
NlaIII CATG 4 cut(s) 458, 560, 1046, 1259
NlaIV GGNNCC 2 cut(s) 768, 850
NmuCI GTSAC 1 cut(s) 216
NspV TTCGAA 1 cut(s) 197
PasI CCCWGGG 1 cut(s) 278
PctI GAATGC 1 cut(s) 874
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 1117
PkrI GCNGC 2 cut(s) 1009, 1231
PleI GAGTC 2 cut(s) 223, 1130
PpsI GAGTC 2 cut(s) 223, 1130
PpuMI RGGWCCY 1 cut(s) 118
PsiI TTATAA 1 cut(s) 1176
Psp5II RGGWCCY 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 768, 850
PspPI GGNCC 3 cut(s) 118, 766, 1086
PspPPI RGGWCCY 1 cut(s) 118
PsrI GAACNNNNNNTAC 2 cut(s) 407, 439
PsuI RGATCY 3 cut(s) 240, 848, 932
RsaI GTAC 3 cut(s) 7, 170, 229
RsaNI GTAC 3 cut(s) 6, 169, 228
SaqAI TTAA 7 cut(s) 123, 132, 252, 314, 543, 1001, 1035
SatI GCNGC 2 cut(s) 1008, 1230
Sau3AI GATC 7 cut(s) 240, 451, 550, 645, 724, 848, 932
Sau96I GGNCC 3 cut(s) 118, 766, 1086
ScaI AGTACT 2 cut(s) 170, 229
SchI GAGTC 2 cut(s) 224, 1130
ScrFI CCNGG 2 cut(s) 279, 603
SfaNI GCATC 2 cut(s) 603, 1153
SfuI TTCGAA 1 cut(s) 197
SinI GGWCC 2 cut(s) 118, 766
SmlI CTYRAG 6 cut(s) 122, 161, 371, 731, 928, 1100
SmoI CTYRAG 6 cut(s) 122, 161, 371, 731, 928, 1100
Sse9I AATT 8 cut(s) 102, 260, 376, 563, 807, 822, 1015, 1080
SspI AATATT 1 cut(s) 841
SspMI CTAG 1 cut(s) 668
StyD4I CCNGG 2 cut(s) 277, 601
TaiI ACGT 3 cut(s) 273, 789, 986
TaqI TCGA 2 cut(s) 197, 953
TasI AATT 8 cut(s) 102, 260, 376, 563, 807, 822, 1015, 1080
TatI WGTACW 2 cut(s) 168, 227
TfiI GAWTC 1 cut(s) 1117
Tru1I TTAA 7 cut(s) 123, 132, 252, 314, 543, 1001, 1035
Tru9I TTAA 7 cut(s) 123, 132, 252, 314, 543, 1001, 1035
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 1 cut(s) 216
TseI GCWGC 2 cut(s) 1007, 1229
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 3 cut(s) 248, 578, 899
TspRI CASTG 1 cut(s) 744
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 118, 766
XapI RAATTY 3 cut(s) 376, 1015, 1080
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 1 cut(s) 668
ZrmI AGTACT 2 cut(s) 170, 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.