Rw5G046860

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006969
Physical Location & Seq
Forward (+)
81185471 .. 81187576
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G046860.1

Sequence Viewer

Length: 1725 bp
ATGAAGAAGATTTGGAGGACGAGGGGGGAGGTGTCCATAGTTGAATGGGAAGGGTCCGACCGATTCATGTTTTCTTTCAAAACTGATTTTGATCGGCGAATGGTGATGAGGGGAAGCCCATGGAATTTTGACAATGCTTTGTTATTGCTCTCAACGACTAATGGGAAATCAGATCAGTGTTCCGTTCCTCTTGAGATGCAGAACTTCTGGATTCTAGTTCGAGGACTTCCTCCAGATTTTCTTTCTCCGGTGATGGCTAGAAGAATAGGAGGTATATTGGGGTACTTTGTTGATCTGGACCCAAAGAAAAAAAGTGATTTTGCAGGTAGTCTGCTGCAAATTCGAGTGGGACTGGATGTAACCCAACCACTTCAAAGATGGGTCAAAATGGATTTAGGGTTTAGTGAGGAAACCAAATTGCAATTGGAGTATGAAGAGCTACCCTACTTTTGTTTCTACTGTGGACGCCTCTCACATGTTAGCTCTAATTGTCCTTGGCATAAAGCAGGGAAGATCAAGGAGATGCAGTATGGAAGGTGGAAAACCCTTGCGAAGAATGTGTTTTGCATTGATCCGGACGGACAAAGAAGGGTGAAAGACCCGGAGCCAACCCTCATTGGATCTGTTCGTTCATGTGATGAGTGGGGTGGGGAGATGGAGATTCAGGAGACTAGAATGGAAGTTGATGGGACGATGGTTGAGATGCAGCCGGAAGCAAAGCGAAGGCGCTTTGAACTTTGTGTCCAAAGCGCGGGGATCGATGTTTTGCCAGAAGTTACGTCCAAGCAAGATGATTGCATGGAGGCGGGAATGGAAGGTAATTCAAATGTTATTCATGTTTCTCAAAAAGACAATGGGCTGGAGGAGTGCGGTAGTGGGCTGTTAGGTTCTAATTGTATATCTAGAGGGTGGCCCAAAGGTGGTAAGGTTGACTACAAACCCCTCAATAGTGGAAGTGCAGGTAAAAGTCCTAGCAAGCTATGTCGTAGTAATGGGGTTAGCATGCAGGAAACTCTGACTTCATTGGACATAAGTGTGTTGAAGAAAGAAGGAAAGAAGGTGATTGTAGGGCAGACAACAGAGCAGAGGGACATAGGTACAATTAAAAAGAAGGGACCCATCACTAGAAATGATCGCTTTGAGCTAGAATGTGCAGGGTTTGGGGAACCCGCACAATTTTCGAGCACTCCAACACCTTTTACAGTGGGAAGTGTGGGTAAGGAAGGGAAGAGTAGTGGTTTGGCTTTGGGTTGGAAAGCGGGGGTTAATCTGAGTATTGTGGGCTCTTCTCAAGCTCATATCGATGCTTTGGGCACGTCTCTTCGGTCTGTTTCATGGGAGCTACTTCGTAAAATAGCTGGTACTGTTCATGGCCCGTGGTTGATTTTTGGCAATTTCAATGAGTTAAGGGAGGCATGGGAGAAGCGGGGAGGAGCTATGAGATCAGAAGCTCAAATGAGGAAGTTCAAAGAGGCTACAGAGGAATGTGGGTTGTTAGAAATCCTCTTTTCTGGTTCCTCGTTCACGTGGTCGAATAGGCATACTTGGGAGAGGCTTGATAGAGGTTTTGTGAATAAAGAAACTTGTTCCTTATGGCCTAATTTGCATGAATACCATGTTGATGTTTTCGCTTCAGATCATCTAGCTCTTATTTTCAACACTAATGGGAAGGGGTTACAGAAGCCTTGGAAGAAGAAACGATATTTCCTGTTTGTACCGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

574

Amino Acids

64.58

Weight (kDa)

8.61

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 1 - 50 7.2e-06 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 118 - 165 2.2e-10 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 314, 950
AccII CGCG 1 cut(s) 752
AccIII TCCGGA 1 cut(s) 574
AciI CCGC 6 cut(s) 752, 806, 870, 1170, 1259, 1426
AclWI GGATC 3 cut(s) 566, 628, 764
AcsI RAATTY 2 cut(s) 124, 339
AcuI CTGAAG 1 cut(s) 1617
AcvI CACGTG 1 cut(s) 1527
AcyI GRCGYC 1 cut(s) 466
AfaI GTAC 4 cut(s) 284, 1099, 1363, 1716
AfiI CCNNNNNNNGG 2 cut(s) 751, 920
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 9 cut(s) 44, 79, 374, 734, 825, 1042, 1399, 1468, 1657
AjiI CACGTC 1 cut(s) 1317
AloI GAACNNNNNNTCC 2 cut(s) 726, 758
Alw21I GWGCWC 1 cut(s) 1187
Alw26I GTCTC 2 cut(s) 662, 1323
AlwI GGATC 3 cut(s) 566, 628, 764
Aor13HI TCCGGA 1 cut(s) 574
AoxI GGCC 3 cut(s) 911, 1372, 1595
ApeKI GCWGC 2 cut(s) 334, 706
ApoI RAATTY 2 cut(s) 124, 339
AspLEI GCGC 2 cut(s) 729, 752
AspS9I GGNCC 5 cut(s) 54, 298, 912, 1115, 1373
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 4 cut(s) 115, 262, 604, 1072
AvaII GGWCC 3 cut(s) 54, 298, 1115
BaeGI GKGCMC 1 cut(s) 1316
BanII GRGCYC 1 cut(s) 1286
BbrPI CACGTG 1 cut(s) 1527
Bbv12I GWGCWC 1 cut(s) 1187
BbvI GCAGC 2 cut(s) 321, 718
BccI CCATC 6 cut(s) 247, 372, 649, 680, 688, 1127
BcgI CGANNNNNNTGC 2 cut(s) 1161, 1195
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 2 cut(s) 662, 1323
BfaI CTAG 8 cut(s) 215, 258, 672, 903, 972, 1125, 1145, 1643
BfmI CTRYAG 1 cut(s) 1476
BfoI RGCGCY 1 cut(s) 730
BfuAI ACCTGC 2 cut(s) 314, 950
BisI GCNGC 2 cut(s) 335, 707
BlsI GCNGC 2 cut(s) 336, 708
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 3 cut(s) 54, 298, 1115
BmgBI CACGTC 1 cut(s) 1317
BmgT120I GGNCC 5 cut(s) 54, 298, 912, 1115, 1373
BmiI GGNNCC 7 cut(s) 55, 300, 606, 1116, 1117, 1167, 1516
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 4 cut(s) 186, 513, 693, 1294
BpmI CTGGAG 2 cut(s) 216, 881
BpuEI CTTGAG 2 cut(s) 212, 1275
BpuMI CCSGG 1 cut(s) 602
Bsa29I ATCGAT 2 cut(s) 759, 1302
BsaAI YACGTR 1 cut(s) 1527
BsaBI GATNNNNATC 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 466
BsaJI CCNNGG 4 cut(s) 119, 494, 1376, 1685
BsaWI WCCGGW 2 cut(s) 247, 574
BsaXI ACNNNNNCTCC 2 cut(s) 512, 542
Bsc4I CCNNNNNNNGG 2 cut(s) 751, 920
Bse1I ACTGG 1 cut(s) 357
Bse8I GATNNNNATC 1 cut(s) 90
BseAI TCCGGA 1 cut(s) 574
BseCI ATCGAT 2 cut(s) 759, 1302
BseDI CCNNGG 4 cut(s) 119, 494, 1376, 1685
BseGI GGATG 1 cut(s) 361
BseJI GATNNNNATC 1 cut(s) 90
BseLI CCNNNNNNNGG 2 cut(s) 751, 920
BseMII CTCAG 1 cut(s) 1262
BseNI ACTGG 1 cut(s) 357
BseRI GAGGAG 2 cut(s) 878, 1446
BseSI GKGCMC 1 cut(s) 1316
BseXI GCAGC 2 cut(s) 321, 718
BsgI GTGCAG 2 cut(s) 978, 1173
Bsh1236I CGCG 1 cut(s) 752
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 3 cut(s) 913, 1374, 1597
BshVI ATCGAT 2 cut(s) 759, 1302
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 1187
BsiSI CCGG 4 cut(s) 248, 575, 602, 710
BslFI GGGAC 4 cut(s) 363, 703, 1103, 1128
BslI CCNNNNNNNGG 2 cut(s) 751, 920
BsmAI GTCTC 2 cut(s) 662, 1323
BsmBI CGTCTC 1 cut(s) 1323
BsmFI GGGAC 4 cut(s) 363, 703, 1103, 1128
BsnI GGCC 3 cut(s) 913, 1374, 1597
Bsp1286I GDGCHC 3 cut(s) 1187, 1286, 1316
Bsp13I TCCGGA 1 cut(s) 574
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 6 cut(s) 752, 806, 870, 1170, 1259, 1426
BspANI GGCC 3 cut(s) 913, 1374, 1597
BspCNI CTCAG 1 cut(s) 1263
BspDI ATCGAT 2 cut(s) 759, 1302
BspEI TCCGGA 1 cut(s) 574
BspFNI CGCG 1 cut(s) 752
BspLI GGNNCC 7 cut(s) 55, 300, 606, 1116, 1117, 1167, 1516
BspMI ACCTGC 2 cut(s) 314, 950
BspPI GGATC 3 cut(s) 566, 628, 764
BspQI GCTCTTC 2 cut(s) 429, 1291
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 4 cut(s) 119, 494, 1376, 1685
BssNI GRCGYC 1 cut(s) 466
BssT1I CCWWGG 3 cut(s) 119, 494, 1685
Bst4CI ACNGT 4 cut(s) 461, 1204, 1366, 1719
Bst6I CTCTTC 4 cut(s) 429, 1223, 1291, 1326
BstACI GRCGYC 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 1527
BstC8I GCNNGC 2 cut(s) 977, 1004
BstDEI CTNAG 1 cut(s) 1271
BstDSI CCRYGG 2 cut(s) 119, 1376
BstF5I GGATG 1 cut(s) 361
BstFNI CGCG 1 cut(s) 752
BstH2I RGCGCY 1 cut(s) 730
BstHHI GCGC 2 cut(s) 729, 752
BstMAI GTCTC 2 cut(s) 662, 1323
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 1 cut(s) 1603
BstNSI RCATGY 2 cut(s) 479, 1006
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 1476
BstSLI GKGCMC 1 cut(s) 1316
BstUI CGCG 1 cut(s) 752
BstV1I GCAGC 2 cut(s) 321, 718
BstX2I RGATCY 1 cut(s) 620
BstYI RGATCY 1 cut(s) 620
Bsu15I ATCGAT 2 cut(s) 759, 1302
BsuRI GGCC 3 cut(s) 913, 1374, 1597
BsuTUI ATCGAT 2 cut(s) 759, 1302
BtgI CCRYGG 2 cut(s) 119, 1376
BtrI CACGTC 1 cut(s) 1317
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 182, 1209
BveI ACCTGC 2 cut(s) 314, 950
Cac8I GCNNGC 2 cut(s) 977, 1004
CfoI GCGC 2 cut(s) 729, 752
Cfr13I GGNCC 5 cut(s) 54, 298, 912, 1115, 1373
ClaI ATCGAT 2 cut(s) 759, 1302
CseI GACGC 1 cut(s) 474
Csp6I GTAC 4 cut(s) 283, 1098, 1362, 1715
CviQI GTAC 4 cut(s) 283, 1098, 1362, 1715
DdeI CTNAG 1 cut(s) 1271
Eam1104I CTCTTC 4 cut(s) 429, 1223, 1291, 1326
EarI CTCTTC 4 cut(s) 429, 1223, 1291, 1326
Eco130I CCWWGG 3 cut(s) 119, 494, 1685
Eco24I GRGCYC 1 cut(s) 1286
Eco47I GGWCC 3 cut(s) 54, 298, 1115
Eco57I CTGAAG 1 cut(s) 1617
Eco72I CACGTG 1 cut(s) 1527
EcoO109I RGGNCCY 1 cut(s) 1115
EcoT14I CCWWGG 3 cut(s) 119, 494, 1685
EcoT38I GRGCYC 1 cut(s) 1286
ErhI CCWWGG 3 cut(s) 119, 494, 1685
Esp3I CGTCTC 1 cut(s) 1323
FaqI GGGAC 4 cut(s) 363, 703, 1103, 1128
FauI CCCGC 5 cut(s) 745, 799, 1177, 1252, 1419
Fnu4HI GCNGC 2 cut(s) 335, 707
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 1286
Fsp4HI GCNGC 2 cut(s) 335, 707
FspBI CTAG 8 cut(s) 215, 258, 672, 903, 972, 1125, 1145, 1643
GlaI GCGC 2 cut(s) 728, 751
GluI GCNGC 2 cut(s) 335, 707
GsuI CTGGAG 2 cut(s) 216, 881
HaeII RGCGCY 1 cut(s) 730
HaeIII GGCC 3 cut(s) 913, 1374, 1597
HapII CCGG 4 cut(s) 248, 575, 602, 710
HgaI GACGC 1 cut(s) 474
HhaI GCGC 2 cut(s) 729, 752
Hin1I GRCGYC 1 cut(s) 466
Hin6I GCGC 2 cut(s) 727, 750
HinP1I GCGC 2 cut(s) 727, 750
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HinfI GANTC 3 cut(s) 63, 211, 661
HpaII CCGG 4 cut(s) 248, 575, 602, 710
HphI GGTGA 4 cut(s) 115, 262, 604, 1072
Hpy166II GTNNAC 3 cut(s) 464, 931, 1524
Hpy188I TCNGA 6 cut(s) 58, 172, 1017, 1272, 1447, 1636
Hpy188III TCNNGA 7 cut(s) 191, 208, 233, 296, 575, 665, 903
Hpy8I GTNNAC 3 cut(s) 464, 931, 1524
HpyCH4III ACNGT 4 cut(s) 461, 1204, 1366, 1719
HpyCH4IV ACGT 3 cut(s) 779, 1316, 1526
HpyF10VI GCNNNNNNNGC 1 cut(s) 1603
HpyF3I CTNAG 1 cut(s) 1271
HpySE526I ACGT 3 cut(s) 779, 1316, 1526
Hsp92I GRCGYC 1 cut(s) 466
HspAI GCGC 2 cut(s) 727, 750
KflI GGGWCCC 1 cut(s) 1115
Kpn2I TCCGGA 1 cut(s) 574
LguI GCTCTTC 2 cut(s) 429, 1291
LmnI GCTCC 3 cut(s) 604, 1339, 1433
Lsp1109I GCAGC 2 cut(s) 321, 718
LweI GCATC 4 cut(s) 186, 513, 693, 1294
MaeI CTAG 8 cut(s) 215, 258, 672, 903, 972, 1125, 1145, 1643
MaeII ACGT 3 cut(s) 779, 1316, 1526
MaeIII GTNAC 3 cut(s) 358, 775, 1674
MfeI CAATTG 1 cut(s) 422
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 3 cut(s) 1187, 1286, 1316
MmeI TCCRAC 3 cut(s) 81, 1214, 1232
MroI TCCGGA 1 cut(s) 574
MseI TTAA 3 cut(s) 1104, 1266, 1406
MslI CAYNNNNRTG 3 cut(s) 1034, 1302, 1620
MspI CCGG 4 cut(s) 248, 575, 602, 710
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 1 cut(s) 422
MvnI CGCG 1 cut(s) 752
MwoI GCNNNNNNNGC 1 cut(s) 1603
NciI CCSGG 1 cut(s) 602
NcoI CCATGG 1 cut(s) 119
NlaIV GGNNCC 7 cut(s) 55, 300, 606, 1116, 1117, 1167, 1516
NspI RCATGY 2 cut(s) 479, 1006
PaeI GCATGC 1 cut(s) 1006
PciI ACATGT 1 cut(s) 475
PciSI GCTCTTC 2 cut(s) 429, 1291
PfeI GAWTC 3 cut(s) 63, 211, 661
PkrI GCNGC 2 cut(s) 336, 708
PmaCI CACGTG 1 cut(s) 1527
PmlI CACGTG 1 cut(s) 1527
Ppu21I YACGTR 1 cut(s) 1527
PpuMI RGGWCCY 1 cut(s) 1115
PscI ACATGT 1 cut(s) 475
Psp5II RGGWCCY 1 cut(s) 1115
PspCI CACGTG 1 cut(s) 1527
PspN4I GGNNCC 7 cut(s) 55, 300, 606, 1116, 1117, 1167, 1516
PspPI GGNCC 5 cut(s) 54, 298, 912, 1115, 1373
PspPPI RGGWCCY 1 cut(s) 1115
PsuI RGATCY 1 cut(s) 620
RsaI GTAC 4 cut(s) 284, 1099, 1363, 1716
RsaNI GTAC 4 cut(s) 283, 1098, 1362, 1715
RseI CAYNNNNRTG 3 cut(s) 1034, 1302, 1620
SapI GCTCTTC 2 cut(s) 429, 1291
SaqAI TTAA 3 cut(s) 1104, 1266, 1406
SatI GCNGC 2 cut(s) 335, 707
Sau96I GGNCC 5 cut(s) 54, 298, 912, 1115, 1373
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 3 cut(s) 1187, 1286, 1316
SfaNI GCATC 4 cut(s) 186, 513, 693, 1294
SfcI CTRYAG 1 cut(s) 1476
SinI GGWCC 3 cut(s) 54, 298, 1115
SmiMI CAYNNNNRTG 3 cut(s) 1034, 1302, 1620
SmlI CTYRAG 2 cut(s) 191, 1290
SmoI CTYRAG 2 cut(s) 191, 1290
SphI GCATGC 1 cut(s) 1006
SsiI CCGC 6 cut(s) 752, 806, 870, 1170, 1259, 1426
SspMI CTAG 8 cut(s) 215, 258, 672, 903, 972, 1125, 1145, 1643
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 3 cut(s) 119, 494, 1685
TaaI ACNGT 4 cut(s) 461, 1204, 1366, 1719
TaiI ACGT 3 cut(s) 782, 1319, 1529
TaqI TCGA 6 cut(s) 220, 343, 759, 1181, 1302, 1532
TaqII GACCGA 2 cut(s) 75, 1314
TfiI GAWTC 3 cut(s) 63, 211, 661
Tru1I TTAA 3 cut(s) 1104, 1266, 1406
Tru9I TTAA 3 cut(s) 1104, 1266, 1406
TscAI CASTG 2 cut(s) 182, 1209
TseI GCWGC 2 cut(s) 334, 706
TspDTI ATGAA 9 cut(s) 17, 55, 447, 621, 824, 1011, 1323, 1358, 1623
TspGWI ACGGA 2 cut(s) 172, 594
TspRI CASTG 2 cut(s) 182, 1209
VpaK11BI GGWCC 3 cut(s) 54, 298, 1115
XapI RAATTY 2 cut(s) 124, 339
XbaI TCTAGA 1 cut(s) 902
XceI RCATGY 2 cut(s) 479, 1006
XcmI CCANNNNNNNNNTGG 2 cut(s) 375, 421
XspI CTAG 8 cut(s) 215, 258, 672, 903, 972, 1125, 1145, 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.