Rh3DG285600

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0003441
Physical Location & Seq
Reverse (-)
29173979 .. 29176241
2263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG285600.1

Sequence Viewer

Length: 1854 bp
ATGGATCCAGAGATATTCGCAGCAGCAGCCGAAGGAAATATTAAAGCCCTTTCAACACATGGGGAGCTTCTGCATGAAATACTCACTCCAACCAAAAACACCGTCCTACATATTTATGTAGCACATGCAAGCTACCCAACCTCAACAAAACTCGGAAAGTTTGACATCTCCAGATATGGCCTCCCTGGTCTACTTGCTGATTTGCTCATTAAAAAGCTAATGCCATACATATGGGACACAGAAAACACAGTTAGGCCAACTACCTTAACTGCATCCCAAGTACAGAGGTCAAAGGAGATTGTCACTCAGTTGCTTGAAGTGTGTCCAGAGCTACTTTTGCAGCCCAACAATAATGGCGACATTGCACTACACATTGCGGCCAGATATGGGCTTGATGGTATAGTTGAAGTTCTTATTGGGGCAGCAAAAGCTCGTCATGGCGATGTTGAGAAACAGGTTGAAGAAGCTTGGCAAATGTTGAGGACACCCAACAAGGAGGAAGACACAGCCCTACACAAGGCAGCGCGCTTTAATCACCTCGGTGTGGTGAAGAGATTGTGCAGAGAATACCCTGATTTCTCCTACTCGGCTAATGTTGCTGGAAAGACTCCAATTTACTTGGCTGCTGAAAGGGGATATCGAGACCTGGTTTCTGAAATACTGGATTCTTGTACTTATCCAGGTTACCAAGGCCCTAGTGGTAAAACAGCTTTACATGCTGCAGTGATCTTCAGTGATATAGAAATGACGCAAAAATTGCTAGACAAGGAGAAGACCCTGGCACAAGCAAAAGATGAACAAGGATGGACTCCACTTCACTTTGCTGCACTCATCGGTAACAGATGGATTGTGAGCCGGCTACTAGATTGTGATAAATCAATTGCCTATATCGTTGACAAAGATGACAAGAAGACAGCTCTTCACATTGCAGCTTCCAAAGGCCATGAAGGCGTACTGACAGAGCTTATATATCGTTGCCCTGATTGTTGTGAAGTAGTCGACCAAAGCCAGAGGAATATCCTTCATTATGCCATTGAGAACAGGAAATTTGAAATAGCTAAGGTTGTTCAAAACCATCCATGGATGAGCAACATCCTTCTAAATGGTAAAGATGTTGCCGGAAACACACCCCTCCATCGACTAGTCAACATACGCCTCCAAACAGCTACTGAATCTTTATTTCGCTATGGAAAAGCTCCTACTCGGTTGAGTCGGTTCATTCATGATGCTAGAGTGGATAAGATGGCTTTCAACAAGGAAGACCTAAATGCTATTGACATCATTCTTGATGATGACCATACTTTATCGGAAGTGGAGAAAGTTGGTCGTTTAGAGGCTTTGGAAGACAGCGGTGCTAGACCAAGTCATCCGATCAACGATGGTGTGAGGCTAGTCGAGAATAACAAAGTTGGGAAAGATACTGAAAAGGAAGCGATAGGTGTTCTTTTGGTAGTGGCTACACTCATAGCAACAGTAACATTCGCAGCAGGTTTCACTGTACCCGGTGGTTACCAAAGTGAGAAAGGAGCTGAGCAGGGTTCTGCTATTTTAACAAGAAATGCAGCTTTCAAAGCATTTGTTATAACAAATACAATAGCCATGATTATCTCCAGCAGCACTGTGTTAGCTTACCTTATTGTCTCCTTACGAGTAGCTTTCCGGAAAATAATTGGTTTGCCATATCTGGAGAATCAAGCAGTTTCAATGTTGTGTGCCATGCTTGCCATGGTAATTGCATTCATTACCGGCACATATGCAGTGTTAGGTACTTCTTCAGTACTTGCCATTGTTGTTTGTGTGGTCGGCTTCAGTTTTTTTCTTGGATGTTCTATTTGGAATTGTAGAGGGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

617

Amino Acids

67.88

Weight (kDa)

6.54

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 163 - 222 8.3e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 216 - 291 6.7e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 238 - 285 5.4e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 299 - 355 1.1e-07 Ankyrin repeats (3 copies)
PGG PF13962 474 - 587 3.4e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1583
Acc36I ACCTGC 1 cut(s) 1478
AccI GTMKAC 2 cut(s) 190, 999
AccII CGCG 1 cut(s) 526
AccIII TCCGGA 1 cut(s) 1659
AciI CCGC 2 cut(s) 377, 1350
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 1 cut(s) 1046
AcuI CTGAAG 3 cut(s) 715, 1758, 1792
AfaI GTAC 6 cut(s) 282, 673, 954, 1500, 1768, 1779
AfiI CCNNNNNNNGG 3 cut(s) 387, 517, 544
AgsI TTSAA 9 cut(s) 54, 317, 407, 461, 1052, 1070, 1252, 1570, 1704
AhlI ACTAGT 1 cut(s) 1141
AjnI CCWGG 4 cut(s) 184, 645, 679, 777
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AleI CACNNNNGTG 1 cut(s) 540
Alw26I GTCTC 2 cut(s) 636, 1645
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 1169
Aor13HI TCCGGA 1 cut(s) 1659
AoxI GGCC 5 cut(s) 178, 254, 378, 691, 940
ApoI RAATTY 1 cut(s) 1046
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AspLEI GCGC 2 cut(s) 526, 528
AspS9I GGNCC 1 cut(s) 692
AsuC2I CCSGG 1 cut(s) 1503
AsuHPI GGTGA 2 cut(s) 527, 559
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 5 cut(s) 507, 779, 917, 1266, 1350
BccI CCATC 7 cut(s) 389, 798, 837, 1083, 1143, 1237, 1373
BciT130I CCWGG 4 cut(s) 186, 647, 681, 779
BcnI CCSGG 1 cut(s) 1503
BcoDI GTCTC 2 cut(s) 636, 1645
BcuI ACTAGT 1 cut(s) 1141
BfaI CTAG 7 cut(s) 696, 761, 863, 1142, 1230, 1356, 1391
BfmI CTRYAG 1 cut(s) 720
BfuAI ACCTGC 1 cut(s) 1478
BglI GCCNNNNNGGC 1 cut(s) 948
BlpI GCTNAGC 1 cut(s) 1530
BmcAI AGTACT 1 cut(s) 1779
Bme1390I CCNGG 5 cut(s) 186, 647, 681, 779, 1503
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 5 cut(s) 186, 647, 681, 779, 1503
BmsI GCATC 2 cut(s) 281, 1216
BpiI GAAGAC 5 cut(s) 507, 779, 917, 1266, 1350
BpmI CTGGAG 3 cut(s) 154, 1594, 1706
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu1102I GCTNAGC 1 cut(s) 1530
BpuMI CCSGG 1 cut(s) 1503
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 6 cut(s) 184, 538, 688, 777, 1079, 1725
BsaWI WCCGGW 1 cut(s) 1659
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 517, 544
Bse118I RCCGGY 2 cut(s) 855, 1745
Bse1I ACTGG 1 cut(s) 666
Bse3DI GCAATG 3 cut(s) 360, 372, 924
BseAI TCCGGA 1 cut(s) 1659
BseBI CCWGG 4 cut(s) 186, 647, 681, 779
BseDI CCNNGG 6 cut(s) 184, 538, 688, 777, 1079, 1725
BseGI GGATG 7 cut(s) 272, 809, 1075, 1089, 1092, 1366, 1829
BseLI CCNNNNNNNGG 3 cut(s) 387, 517, 544
BseMI GCAATG 3 cut(s) 360, 372, 924
BseMII CTCAG 2 cut(s) 320, 1521
BseNI ACTGG 1 cut(s) 666
BsePI GCGCGC 1 cut(s) 524
BsgI GTGCAG 2 cut(s) 580, 810
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 5 cut(s) 180, 256, 380, 693, 942
BsiSI CCGG 5 cut(s) 856, 1119, 1503, 1660, 1746
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 387, 517, 544
BsmAI GTCTC 2 cut(s) 636, 1645
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 1736
BsnI GGCC 5 cut(s) 180, 256, 380, 693, 942
Bso31I GGTCTC 1 cut(s) 636
Bsp13I TCCGGA 1 cut(s) 1659
Bsp143I GATC 3 cut(s) 4, 726, 1371
Bsp1720I GCTNAGC 1 cut(s) 1530
Bsp19I CCATGG 2 cut(s) 1079, 1725
BspACI CCGC 2 cut(s) 377, 1350
BspANI GGCC 5 cut(s) 180, 256, 380, 693, 942
BspCNI CTCAG 2 cut(s) 319, 1522
BspEI TCCGGA 1 cut(s) 1659
BspFNI CGCG 1 cut(s) 526
BspHI TCATGA 1 cut(s) 1222
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 724
BspMI ACCTGC 1 cut(s) 1478
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 924
BspTNI GGTCTC 1 cut(s) 636
BsrDI GCAATG 3 cut(s) 360, 372, 924
BsrFI RCCGGY 2 cut(s) 855, 1745
BsrI ACTGG 1 cut(s) 666
BssAI RCCGGY 2 cut(s) 855, 1745
BssECI CCNNGG 6 cut(s) 184, 538, 688, 777, 1079, 1725
BssHII GCGCGC 1 cut(s) 524
BssMI GATC 3 cut(s) 4, 726, 1371
BssT1I CCWWGG 3 cut(s) 688, 1079, 1725
Bst2UI CCWGG 4 cut(s) 186, 647, 681, 779
Bst4CI ACNGT 5 cut(s) 103, 250, 1474, 1498, 1621
Bst6I CTCTTC 2 cut(s) 545, 924
BstAPI GCANNNNNTGC 1 cut(s) 757
BstC8I GCNNGC 4 cut(s) 130, 526, 857, 1722
BstDEI CTNAG 3 cut(s) 306, 1059, 1530
BstDSI CCRYGG 2 cut(s) 1079, 1725
BstEII GGTNACC 2 cut(s) 683, 1508
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 7 cut(s) 272, 809, 1075, 1089, 1092, 1366, 1829
BstFNI CGCG 1 cut(s) 526
BstHHI GCGC 2 cut(s) 526, 528
BstKTI GATC 3 cut(s) 7, 729, 1374
BstMAI GTCTC 2 cut(s) 636, 1645
BstMBI GATC 3 cut(s) 4, 726, 1371
BstMWI GCNNNNNNNGC 9 cut(s) 26, 337, 428, 596, 716, 757, 948, 1571, 1721
BstNI CCWGG 4 cut(s) 186, 647, 681, 779
BstNSI RCATGY 2 cut(s) 128, 719
BstPI GGTNACC 2 cut(s) 683, 1508
BstSCI CCNGG 5 cut(s) 184, 645, 679, 777, 1501
BstSFI CTRYAG 1 cut(s) 720
BstUI CGCG 1 cut(s) 526
BstV2I GAAGAC 5 cut(s) 507, 779, 917, 1266, 1350
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 231
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 5 cut(s) 180, 256, 380, 693, 942
BtgI CCRYGG 2 cut(s) 1079, 1725
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 7 cut(s) 272, 809, 1075, 1089, 1092, 1366, 1829
BtsI GCAGTG 2 cut(s) 729, 1764
BtsIMutI CAGTG 5 cut(s) 729, 739, 1494, 1617, 1764
BveI ACCTGC 1 cut(s) 1478
Cac8I GCNNGC 4 cut(s) 130, 526, 857, 1722
CaiI CAGNNNCTG 1 cut(s) 1169
CciI TCATGA 1 cut(s) 1222
CfoI GCGC 2 cut(s) 526, 528
Cfr10I RCCGGY 2 cut(s) 855, 1745
Cfr13I GGNCC 1 cut(s) 692
CseI GACGC 1 cut(s) 757
CsiI ACCWGGT 1 cut(s) 645
Csp6I GTAC 6 cut(s) 281, 672, 953, 1499, 1767, 1778
CviQI GTAC 6 cut(s) 281, 672, 953, 1499, 1767, 1778
DdeI CTNAG 3 cut(s) 306, 1059, 1530
DpnI GATC 3 cut(s) 6, 728, 1373
DpnII GATC 3 cut(s) 4, 726, 1371
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 2 cut(s) 545, 924
EarI CTCTTC 2 cut(s) 545, 924
Eco130I CCWWGG 3 cut(s) 688, 1079, 1725
Eco31I GGTCTC 1 cut(s) 636
Eco32I GATATC 1 cut(s) 638
Eco57I CTGAAG 3 cut(s) 715, 1758, 1792
Eco91I GGTNACC 2 cut(s) 683, 1508
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoO109I RGGNCCY 1 cut(s) 692
EcoO65I GGTNACC 2 cut(s) 683, 1508
EcoRII CCWGG 4 cut(s) 184, 645, 679, 777
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 3 cut(s) 688, 1079, 1725
ErhI CCWWGG 3 cut(s) 688, 1079, 1725
FaqI GGGAC 1 cut(s) 248
FauNDI CATATG 2 cut(s) 230, 1753
FblI GTMKAC 2 cut(s) 190, 999
FokI GGATG 7 cut(s) 259, 816, 1062, 1079, 1096, 1353, 1836
FspBI CTAG 7 cut(s) 696, 761, 863, 1142, 1230, 1356, 1391
GlaI GCGC 2 cut(s) 525, 527
GsuI CTGGAG 3 cut(s) 154, 1594, 1706
HaeIII GGCC 5 cut(s) 180, 256, 380, 693, 942
HapII CCGG 5 cut(s) 856, 1119, 1503, 1660, 1746
HgaI GACGC 1 cut(s) 757
HhaI GCGC 2 cut(s) 526, 528
Hin6I GCGC 2 cut(s) 524, 526
HinP1I GCGC 2 cut(s) 524, 526
HincII GTYRAC 3 cut(s) 895, 1000, 1147
HindII GTYRAC 3 cut(s) 895, 1000, 1147
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 6 cut(s) 607, 665, 808, 1172, 1210, 1690
HpaII CCGG 5 cut(s) 856, 1119, 1503, 1660, 1746
HphI GGTGA 2 cut(s) 527, 559
Hpy166II GTNNAC 4 cut(s) 191, 895, 1000, 1147
Hpy188I TCNGA 4 cut(s) 155, 655, 1309, 1371
Hpy8I GTNNAC 4 cut(s) 191, 895, 1000, 1147
HpyAV CCTTC 4 cut(s) 26, 941, 1031, 1106
HpyCH4III ACNGT 5 cut(s) 103, 250, 1474, 1498, 1621
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 337, 428, 596, 716, 757, 948, 1571, 1721
HpyF3I CTNAG 3 cut(s) 306, 1059, 1530
HspAI GCGC 2 cut(s) 524, 526
Kpn2I TCCGGA 1 cut(s) 1659
KroI GCCGGC 1 cut(s) 855
KroNI GCCGGC 1 cut(s) 857
Kzo9I GATC 3 cut(s) 4, 726, 1371
LguI GCTCTTC 1 cut(s) 924
LmnI GCTCC 3 cut(s) 64, 1201, 1526
LweI GCATC 2 cut(s) 281, 1216
MabI ACCWGGT 1 cut(s) 645
MaeI CTAG 7 cut(s) 696, 761, 863, 1142, 1230, 1356, 1391
MaeIII GTNAC 5 cut(s) 301, 683, 836, 1474, 1508
MalI GATC 3 cut(s) 6, 728, 1373
MboI GATC 3 cut(s) 4, 726, 1371
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 1 cut(s) 4
MluCI AATT 7 cut(s) 612, 755, 879, 1046, 1668, 1731, 1837
MlyI GAGTC 3 cut(s) 601, 802, 1219
MmeI TCCRAC 1 cut(s) 113
MroI TCCGGA 1 cut(s) 1659
MroNI GCCGGC 1 cut(s) 855
MseI TTAA 5 cut(s) 42, 210, 266, 531, 1550
MslI CAYNNNNRTG 4 cut(s) 114, 229, 441, 540
MspA1I CMGCKG 1 cut(s) 1350
MspI CCGG 5 cut(s) 856, 1119, 1503, 1660, 1746
MspR9I CCNGG 5 cut(s) 186, 647, 681, 779, 1503
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 1 cut(s) 1736
MvaI CCWGG 4 cut(s) 186, 647, 681, 779
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 9 cut(s) 26, 337, 428, 596, 716, 757, 948, 1571, 1721
NaeI GCCGGC 1 cut(s) 857
NciI CCSGG 1 cut(s) 1503
NcoI CCATGG 2 cut(s) 1079, 1725
NdeI CATATG 2 cut(s) 230, 1753
NdeII GATC 3 cut(s) 4, 726, 1371
NgoMIV GCCGGC 1 cut(s) 855
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 301
NspI RCATGY 2 cut(s) 128, 719
OliI CACNNNNGTG 1 cut(s) 540
PagI TCATGA 1 cut(s) 1222
PauI GCGCGC 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 924
PctI GAATGC 1 cut(s) 1736
PdiI GCCGGC 1 cut(s) 857
PfeI GAWTC 3 cut(s) 665, 1172, 1690
PflFI GACNNNGTC 1 cut(s) 1362
PleI GAGTC 3 cut(s) 601, 802, 1218
PpsI GAGTC 3 cut(s) 601, 802, 1218
PsiI TTATAA 1 cut(s) 1583
Psp6I CCWGG 4 cut(s) 184, 645, 679, 777
PspEI GGTNACC 2 cut(s) 683, 1508
PspGI CCWGG 4 cut(s) 184, 645, 679, 777
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 692
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 1169
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 1362
PteI GCGCGC 1 cut(s) 524
RsaI GTAC 6 cut(s) 282, 673, 954, 1500, 1768, 1779
RsaNI GTAC 6 cut(s) 281, 672, 953, 1499, 1767, 1778
RseI CAYNNNNRTG 4 cut(s) 114, 229, 441, 540
SalI GTCGAC 1 cut(s) 998
SapI GCTCTTC 1 cut(s) 924
SaqAI TTAA 5 cut(s) 42, 210, 266, 531, 1550
Sau3AI GATC 3 cut(s) 4, 726, 1371
Sau96I GGNCC 1 cut(s) 692
ScaI AGTACT 1 cut(s) 1779
SchI GAGTC 3 cut(s) 601, 802, 1219
ScrFI CCNGG 5 cut(s) 186, 647, 681, 779, 1503
SexAI ACCWGGT 1 cut(s) 645
SfaNI GCATC 2 cut(s) 281, 1216
SfcI CTRYAG 1 cut(s) 720
SmiMI CAYNNNNRTG 4 cut(s) 114, 229, 441, 540
SpeI ACTAGT 1 cut(s) 1141
Sse9I AATT 7 cut(s) 612, 755, 879, 1046, 1668, 1731, 1837
SsiI CCGC 2 cut(s) 377, 1350
SspI AATATT 1 cut(s) 40
SspMI CTAG 7 cut(s) 696, 761, 863, 1142, 1230, 1356, 1391
StyD4I CCNGG 5 cut(s) 184, 645, 679, 777, 1501
StyI CCWWGG 3 cut(s) 688, 1079, 1725
TaaI ACNGT 5 cut(s) 103, 250, 1474, 1498, 1621
TaqI TCGA 4 cut(s) 640, 999, 1138, 1395
TasI AATT 7 cut(s) 612, 755, 879, 1046, 1668, 1731, 1837
TatI WGTACW 3 cut(s) 280, 671, 1777
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 3 cut(s) 665, 1172, 1690
Tru1I TTAA 5 cut(s) 42, 210, 266, 531, 1550
Tru9I TTAA 5 cut(s) 42, 210, 266, 531, 1550
TscAI CASTG 5 cut(s) 729, 739, 1501, 1624, 1764
TseFI GTSAC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 7 cut(s) 90, 810, 960, 1013, 1207, 1211, 1729
TspRI CASTG 5 cut(s) 729, 739, 1501, 1624, 1764
Tth111I GACNNNGTC 1 cut(s) 1362
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 1 cut(s) 1046
XceI RCATGY 2 cut(s) 128, 719
XcmI CCANNNNNNNNNTGG 2 cut(s) 695, 1723
XmiI GTMKAC 2 cut(s) 190, 999
XspI CTAG 7 cut(s) 696, 761, 863, 1142, 1230, 1356, 1391
ZrmI AGTACT 1 cut(s) 1779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.