Rh4CG333600

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007526
Physical Location & Seq
Forward (+)
59433300 .. 59446895
13596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG333600.1

Sequence Viewer

Length: 1062 bp
ATGGCAGAGAGGAATCAAGAGGCTTACCCTTTCGCACCATACGCTAATGGGCAAGCCATGGCCAGAAGCGATGCAGAATCTTCGAGAGCTCACTCCGATGATGAGCTACGCAGAAAAAAGCGCATCAAATGTCTAATCTACATCGCGGTTTTCGCTGTGTTCCAGATCATAGTCATAACTGTTTTCGCACTCACTGTCATGCGTGTCAAGAGCCCCAAGTTCCGTATCCAAGCCATCACGGTTGAGGAACTCAACACCACGGCCAATCCCTCGCTTACTATGAGGGCGTCGTTGGATTCGCTGATCGCCGGGTTCAACAGTCGGATCTCAGAGCTCCAAGATCTCGTTATTGAGCGAAACATGTACCCAGCCAGCAGCGTCACCGATTTGTCGGCCGTCGACGCCGCTGTGAAGGACATGGAGCTTCAGGCCCAGGTCAGCAAGGACCGGGTTCGTGACGAAACCCTAGCCATCCCCAAAGCAAAAGTAGTTCCGACGTCTGAATATTCTGTTACATCTCTCAGCTCTCTGGCTTTGGCGGCATTCTTAAGCAAAGGTATTCTGATTTTATGCTCCACCAGAGGTAGTGAAGGAAAATGGAGCGAAGGCGCAGGACACTGCAAGAAGCTTTGGTCTCATCTTTATAAGGATACACAGGAAGCTTTGGGAATTATAGGAAAGATGCTTGGAATACAGCGATTGAGCAGTTCTGGGTACGTCTTTTCTACTTCTTTGCCCCCATATCTTGCGAGTGCTACCATTACTGTCATTGATGTTCTTGAGGAGAACCCTGATCTGATAACGAAGTTGAAGAAAAACATTACGTTACTATGTAAAGGTCTGTGCTTGATCTCATTTTTCATGTGCATGTGTGTTTCTCTTGGTTTTATATCCCACGGCCAGTATAGTCATCACTTGATTAGTAGTGTCTTAGCAGTCGCTTATCACATGCCTTGTGCTGGCATCCTCTTGATTGAGTATTGTGCTGTGATGTGCAGGATTGTCAGGTGTAATGGGGGTTGCTTATATTCAAGAAGTTGGGAAAGCCAGCCTCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

353

Amino Acids

38.89

Weight (kDa)

8.65

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 97 - 163 9.5e-11 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AatII GACGTC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 399
AccII CGCG 1 cut(s) 146
AciI CCGC 3 cut(s) 146, 405, 539
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 4 cut(s) 60, 261, 393, 898
AcuI CTGAAG 1 cut(s) 410
AcyI GRCGYC 3 cut(s) 287, 402, 497
AfaI GTAC 2 cut(s) 365, 716
AfiI CCNNNNNNNGG 1 cut(s) 959
AflII CTTAAG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 3 cut(s) 316, 811, 1032
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 7 cut(s) 89, 106, 334, 424, 525, 628, 662
AluI AGCT 7 cut(s) 89, 106, 334, 424, 525, 628, 662
Alw21I GWGCWC 2 cut(s) 91, 336
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 1 cut(s) 332
AoxI GGCC 5 cut(s) 60, 261, 393, 429, 898
ApeKI GCWGC 1 cut(s) 375
AspLEI GCGC 2 cut(s) 123, 611
AspS9I GGNCC 2 cut(s) 430, 445
AsuC2I CCSGG 2 cut(s) 310, 449
AsuHPI GGTGA 1 cut(s) 373
AvaII GGWCC 1 cut(s) 445
BalI TGGCCA 1 cut(s) 62
BanII GRGCYC 3 cut(s) 91, 215, 336
Bbv12I GWGCWC 2 cut(s) 91, 336
BbvI GCAGC 1 cut(s) 387
BccI CCATC 2 cut(s) 242, 479
BceAI ACGGC 3 cut(s) 276, 380, 913
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 1 cut(s) 434
BciVI GTATCC 2 cut(s) 236, 643
BcnI CCSGG 2 cut(s) 310, 449
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 1 cut(s) 467
BfrI CTTAAG 1 cut(s) 547
BfuI GTATCC 2 cut(s) 236, 643
BglII AGATCT 1 cut(s) 340
BisI GCNGC 3 cut(s) 376, 405, 540
BlsI GCNGC 3 cut(s) 377, 406, 541
Bme1390I CCNGG 3 cut(s) 310, 434, 449
Bme18I GGWCC 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 430, 445
BmrFI CCNGG 3 cut(s) 310, 434, 449
BmsI GCATC 4 cut(s) 61, 132, 672, 972
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 2 cut(s) 310, 449
BsaHI GRCGYC 3 cut(s) 287, 402, 497
BsaI GGTCTC 1 cut(s) 639
BsaJI CCNNGG 4 cut(s) 57, 258, 432, 895
Bsc4I CCNNNNNNNGG 1 cut(s) 959
Bse1I ACTGG 1 cut(s) 901
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 4 cut(s) 57, 258, 432, 895
BseGI GGATG 2 cut(s) 471, 963
BseLI CCNNNNNNNGG 1 cut(s) 959
BseMII CTCAG 2 cut(s) 342, 535
BseNI ACTGG 1 cut(s) 901
BseRI GAGGAG 1 cut(s) 797
BseX3I CGGCCG 1 cut(s) 393
BseXI GCAGC 1 cut(s) 387
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 1015
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 5 cut(s) 62, 263, 395, 431, 900
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 91, 336
BsiSI CCGG 2 cut(s) 309, 448
BslI CCNNNNNNNGG 1 cut(s) 959
BsmAI GTCTC 1 cut(s) 639
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 5 cut(s) 62, 263, 395, 431, 900
Bso31I GGTCTC 1 cut(s) 639
Bsp1286I GDGCHC 3 cut(s) 91, 215, 336
Bsp143I GATC 6 cut(s) 165, 303, 324, 340, 793, 849
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 146, 405, 539
BspANI GGCC 5 cut(s) 62, 263, 395, 431, 900
BspCNI CTCAG 2 cut(s) 341, 534
BspFNI CGCG 1 cut(s) 146
BspPI GGATC 1 cut(s) 332
BspTI CTTAAG 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 639
BsrI ACTGG 1 cut(s) 901
BssECI CCNNGG 4 cut(s) 57, 258, 432, 895
BssMI GATC 6 cut(s) 165, 303, 324, 340, 793, 849
BssNI GRCGYC 3 cut(s) 287, 402, 497
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 5 cut(s) 181, 196, 241, 320, 766
BstACI GRCGYC 3 cut(s) 287, 402, 497
BstAFI CTTAAG 1 cut(s) 547
BstC8I GCNNGC 4 cut(s) 54, 373, 961, 1049
BstDEI CTNAG 3 cut(s) 328, 521, 931
BstDSI CCRYGG 3 cut(s) 57, 258, 895
BstF5I GGATG 2 cut(s) 471, 963
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 123, 611
BstKTI GATC 6 cut(s) 168, 306, 327, 343, 796, 852
BstMAI GTCTC 1 cut(s) 639
BstMBI GATC 6 cut(s) 165, 303, 324, 340, 793, 849
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 4 cut(s) 41, 152, 401, 539
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 3 cut(s) 364, 871, 952
BstSCI CCNGG 3 cut(s) 308, 432, 447
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 324, 340
BstYI RGATCY 2 cut(s) 324, 340
BstZI CGGCCG 1 cut(s) 393
BsuI GTATCC 2 cut(s) 236, 643
BsuRI GGCC 5 cut(s) 62, 263, 395, 431, 900
BtgI CCRYGG 3 cut(s) 57, 258, 895
BtgZI GCGATG 2 cut(s) 84, 127
BtsCI GGATG 2 cut(s) 471, 963
BtsI GCAGTG 1 cut(s) 616
BtsIMutI CAGTG 2 cut(s) 192, 616
Cac8I GCNNGC 4 cut(s) 54, 373, 961, 1049
CfoI GCGC 2 cut(s) 123, 611
Cfr13I GGNCC 2 cut(s) 430, 445
CseI GACGC 3 cut(s) 276, 367, 410
Csp6I GTAC 2 cut(s) 364, 715
CviAII CATG 7 cut(s) 58, 199, 361, 418, 862, 868, 949
CviQI GTAC 2 cut(s) 364, 715
DdeI CTNAG 3 cut(s) 328, 521, 931
DpnI GATC 6 cut(s) 167, 305, 326, 342, 795, 851
DpnII GATC 6 cut(s) 165, 303, 324, 340, 793, 849
EaeI YGGCCR 4 cut(s) 60, 261, 393, 898
EagI CGGCCG 1 cut(s) 393
Ecl136II GAGCTC 2 cut(s) 89, 334
EclXI CGGCCG 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 3 cut(s) 91, 215, 336
Eco31I GGTCTC 1 cut(s) 639
Eco47I GGWCC 1 cut(s) 445
Eco52I CGGCCG 1 cut(s) 393
Eco53kI GAGCTC 2 cut(s) 89, 334
Eco57I CTGAAG 1 cut(s) 410
EcoICRI GAGCTC 2 cut(s) 89, 334
EcoRII CCWGG 1 cut(s) 432
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 3 cut(s) 91, 215, 336
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 7 cut(s) 61, 202, 364, 421, 865, 871, 952
FatI CATG 7 cut(s) 57, 198, 360, 417, 861, 867, 948
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 376, 405, 540
FokI GGATG 2 cut(s) 458, 950
FriOI GRGCYC 3 cut(s) 91, 215, 336
Fsp4HI GCNGC 3 cut(s) 376, 405, 540
FspBI CTAG 1 cut(s) 467
GlaI GCGC 2 cut(s) 122, 610
GluI GCNGC 3 cut(s) 376, 405, 540
GsaI CCCAGC 1 cut(s) 371
HaeIII GGCC 5 cut(s) 62, 263, 395, 431, 900
HapII CCGG 2 cut(s) 309, 448
HgaI GACGC 3 cut(s) 276, 367, 410
HhaI GCGC 2 cut(s) 123, 611
Hin1I GRCGYC 3 cut(s) 287, 402, 497
Hin1II CATG 7 cut(s) 61, 202, 364, 421, 865, 871, 952
Hin6I GCGC 2 cut(s) 121, 609
HinP1I GCGC 2 cut(s) 121, 609
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 2 cut(s) 626, 660
HinfI GANTC 3 cut(s) 13, 77, 296
HpaII CCGG 2 cut(s) 309, 448
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 7 cut(s) 97, 324, 331, 495, 502, 564, 798
Hpy188III TCNNGA 8 cut(s) 17, 84, 163, 208, 455, 779, 970, 1032
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 4 cut(s) 292, 401, 404, 499
HpyAV CCTTC 3 cut(s) 406, 584, 599
HpyCH4III ACNGT 5 cut(s) 181, 196, 241, 320, 766
HpyCH4IV ACGT 3 cut(s) 497, 717, 824
HpyCH4V TGCA 4 cut(s) 74, 621, 867, 996
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 152, 401, 539
HpyF3I CTNAG 3 cut(s) 328, 521, 931
HpySE526I ACGT 3 cut(s) 497, 717, 824
Hsp92I GRCGYC 3 cut(s) 287, 402, 497
Hsp92II CATG 7 cut(s) 61, 202, 364, 421, 865, 871, 952
HspAI GCGC 2 cut(s) 121, 609
Kzo9I GATC 6 cut(s) 165, 303, 324, 340, 793, 849
LmnI GCTCC 4 cut(s) 339, 421, 578, 600
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 4 cut(s) 61, 132, 672, 972
MaeI CTAG 1 cut(s) 467
MaeII ACGT 3 cut(s) 497, 717, 824
MaeIII GTNAC 4 cut(s) 379, 455, 511, 825
MalI GATC 6 cut(s) 167, 305, 326, 342, 795, 851
MboI GATC 6 cut(s) 165, 303, 324, 340, 793, 849
MboII GAAGA 2 cut(s) 72, 823
MflI RGATCY 2 cut(s) 324, 340
MhlI GDGCHC 3 cut(s) 91, 215, 336
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 1 cut(s) 669
MluNI TGGCCA 1 cut(s) 62
MmeI TCCRAC 3 cut(s) 273, 302, 518
MnlI CCTC 9 cut(s) 3, 13, 238, 276, 280, 575, 775, 977, 1062
Mox20I TGGCCA 1 cut(s) 62
MscI TGGCCA 1 cut(s) 62
MseI TTAA 1 cut(s) 548
MslI CAYNNNNRTG 3 cut(s) 96, 197, 866
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 407
MspCI CTTAAG 1 cut(s) 547
MspI CCGG 2 cut(s) 309, 448
MspR9I CCNGG 3 cut(s) 310, 434, 449
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 4 cut(s) 41, 152, 401, 539
NciI CCSGG 2 cut(s) 310, 449
NcoI CCATGG 1 cut(s) 57
NdeII GATC 6 cut(s) 165, 303, 324, 340, 793, 849
NlaIII CATG 7 cut(s) 61, 202, 364, 421, 865, 871, 952
NmuCI GTSAC 2 cut(s) 379, 455
NspI RCATGY 3 cut(s) 364, 871, 952
PciI ACATGT 1 cut(s) 360
PctI GAATGC 1 cut(s) 542
PfeI GAWTC 3 cut(s) 13, 77, 296
PkrI GCNGC 3 cut(s) 377, 406, 541
PscI ACATGT 1 cut(s) 360
PsiI TTATAA 1 cut(s) 645
Psp124BI GAGCTC 2 cut(s) 91, 336
Psp6I CCWGG 1 cut(s) 432
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 432
PspPI GGNCC 2 cut(s) 430, 445
PsuI RGATCY 2 cut(s) 324, 340
RsaI GTAC 2 cut(s) 365, 716
RsaNI GTAC 2 cut(s) 364, 715
RseI CAYNNNNRTG 3 cut(s) 96, 197, 866
SacI GAGCTC 2 cut(s) 91, 336
SalI GTCGAC 1 cut(s) 398
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 3 cut(s) 376, 405, 540
Sau3AI GATC 6 cut(s) 165, 303, 324, 340, 793, 849
Sau96I GGNCC 2 cut(s) 430, 445
ScrFI CCNGG 3 cut(s) 310, 434, 449
SduI GDGCHC 3 cut(s) 91, 215, 336
SfaNI GCATC 4 cut(s) 61, 132, 672, 972
SgrDI CGTCGACG 1 cut(s) 398
SinI GGWCC 1 cut(s) 445
SmiMI CAYNNNNRTG 3 cut(s) 96, 197, 866
SmlI CTYRAG 2 cut(s) 547, 779
SmoI CTYRAG 2 cut(s) 547, 779
Sse9I AATT 1 cut(s) 669
SsiI CCGC 3 cut(s) 146, 405, 539
SspI AATATT 1 cut(s) 506
SspMI CTAG 1 cut(s) 467
SstI GAGCTC 2 cut(s) 91, 336
StyD4I CCNGG 3 cut(s) 308, 432, 447
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 5 cut(s) 181, 196, 241, 320, 766
TaiI ACGT 3 cut(s) 500, 720, 827
TaqI TCGA 2 cut(s) 83, 399
TasI AATT 1 cut(s) 669
TauI GCSGC 2 cut(s) 407, 542
TfiI GAWTC 3 cut(s) 13, 77, 296
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TscAI CASTG 2 cut(s) 199, 623
TseFI GTSAC 2 cut(s) 379, 455
TseI GCWGC 1 cut(s) 375
Tsp45I GTSAC 2 cut(s) 379, 455
TspDTI ATGAA 1 cut(s) 850
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 2 cut(s) 199, 623
Vha464I CTTAAG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 445
XceI RCATGY 3 cut(s) 364, 871, 952
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 1 cut(s) 467
ZraI GACGTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.