Rh4DG085100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002689
Physical Location & Seq
Forward (+)
12951294 .. 12954661
3368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG085100.1

Sequence Viewer

Length: 735 bp
ATGAGTTCCCTCTTTTGCAGTTTCAGTTCAGTTGGTTCACTAGAAGAGTTGGTTGGTAACAGAAACTTTGTTTCCGGGTACTTTGATTTACAGAGCTGGTACTTTGATTTGACACATTGTACATTGATATCGAAACTAAGGTTGCAACTATGTCTGCCGGTGCAGATATCTTCTCTAGAGGTTGAATCTGCAGGTGTTTGTGTTGAGTATAGATATCATAACATTATTCTCACAAAGATCAAGATTATTATGGGCCAGGTTCCTTCATCACGCTTTCACACACCTGCTGAAGAGCAGGGACCTAAGTCACCTAACCGTCTTCAAGCACAAGAGGCAGAAAGGCAAAGTATAAATATTCCCCATGGAGAGACGAGACAATCAATGAGTCATTCGCAATCTCTTTCATCACACTATTACCCACCCAATGAAGACCAGGGTTACACCCATCTTCATGCATTAGAGGCTGAAAGGCAGCGTAGAAATAATCGTCAAAGAGAGAGGCGACAGTTATTGAGTTCTTCACAACGAGCGGAAATTTTAGCCCAAAGACGTGCCAACTATCAGATACAACGACAAATGGAGAACACAAGTGTAACAAATCAAGTGGAAGCAATGGACGGTAACTTACTGAGTGCATGTTCCGAAACACAAACATCTTCAATGTATTGTATATATATTTTTTTTCACAATTTTCTTTCTAATTTGATCAACAAAAATATTCTTTTGCATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

28.12

Weight (kDa)

7.7

Isoelectric Point (pI)

70.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 182, 292
Acc36I ACCTGC 2 cut(s) 182, 292
AccBSI CCGCTC 1 cut(s) 530
AciI CCGC 1 cut(s) 530
AcsI RAATTY 1 cut(s) 534
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 3 cut(s) 80, 101, 121
AgsI TTSAA 3 cut(s) 185, 323, 660
AjiI CACGTC 1 cut(s) 551
AjnI CCWGG 2 cut(s) 255, 432
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AluBI AGCT 1 cut(s) 96
AluI AGCT 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 362, 367
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 1 cut(s) 534
AspS9I GGNCC 2 cut(s) 253, 299
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 300
AvaII GGWCC 1 cut(s) 299
BbsI GAAGAC 2 cut(s) 311, 435
BbvI GCAGC 1 cut(s) 484
BccI CCATC 1 cut(s) 453
BciT130I CCWGG 2 cut(s) 257, 434
BclI TGATCA 1 cut(s) 705
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 362, 367
BfaI CTAG 2 cut(s) 41, 176
BfmI CTRYAG 1 cut(s) 189
BfuAI ACCTGC 2 cut(s) 182, 292
BisI GCNGC 1 cut(s) 473
BlsI GCNGC 1 cut(s) 474
Bme1390I CCNGG 3 cut(s) 76, 257, 434
Bme18I GGWCC 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 551
BmgT120I GGNCC 2 cut(s) 253, 299
BmiI GGNNCC 2 cut(s) 261, 300
BmrFI CCNGG 3 cut(s) 76, 257, 434
BoxI GACNNNNGTC 1 cut(s) 304
BpiI GAAGAC 2 cut(s) 311, 435
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 361, 433
Bse118I RCCGGY 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 618
BseBI CCWGG 2 cut(s) 257, 434
BseDI CCNNGG 2 cut(s) 361, 433
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 1 cut(s) 620
BseXI GCAGC 1 cut(s) 484
BsgI GTGCAG 1 cut(s) 182
BshFI GGCC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 75, 158
BslFI GGGAC 1 cut(s) 312
BsmAI GTCTC 2 cut(s) 362, 367
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 119
Bsp143I GATC 2 cut(s) 237, 705
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 1 cut(s) 530
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 261, 300
BspMAI CTGCAG 1 cut(s) 193
BspMI ACCTGC 2 cut(s) 182, 292
BspQI GCTCTTC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 618
BsrFI RCCGGY 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 361, 433
BssMI GATC 2 cut(s) 237, 705
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 2 cut(s) 257, 434
Bst4CI ACNGT 3 cut(s) 317, 507, 620
Bst6I CTCTTC 2 cut(s) 39, 285
BstAUI TGTACA 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 137, 303, 629
BstDSI CCRYGG 1 cut(s) 361
BstKTI GATC 2 cut(s) 240, 708
BstMAI GTCTC 2 cut(s) 362, 367
BstMBI GATC 2 cut(s) 237, 705
BstMWI GCNNNNNNNGC 2 cut(s) 332, 461
BstNI CCWGG 2 cut(s) 257, 434
BstNSI RCATGY 1 cut(s) 639
BstPAI GACNNNNGTC 1 cut(s) 304
BstSCI CCNGG 3 cut(s) 74, 255, 432
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 484
BstV2I GAAGAC 2 cut(s) 311, 435
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 361
BtrI CACGTC 1 cut(s) 551
BveI ACCTGC 2 cut(s) 182, 292
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 253, 299
Csp6I GTAC 3 cut(s) 79, 100, 120
CspCI CAANNNNNGTGG 2 cut(s) 585, 620
CviAII CATG 3 cut(s) 362, 452, 636
CviJI RGCY 4 cut(s) 96, 255, 464, 542
CviKI_1 RGCY 4 cut(s) 96, 255, 464, 542
CviQI GTAC 3 cut(s) 79, 100, 120
DdeI CTNAG 3 cut(s) 137, 303, 629
DpnI GATC 2 cut(s) 239, 707
DpnII GATC 2 cut(s) 237, 705
Eam1104I CTCTTC 2 cut(s) 39, 285
EarI CTCTTC 2 cut(s) 39, 285
Eco130I CCWWGG 1 cut(s) 361
Eco32I GATATC 3 cut(s) 129, 168, 215
Eco47I GGWCC 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 255, 432
EcoRV GATATC 3 cut(s) 129, 168, 215
EcoT14I CCWWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 3 cut(s) 365, 455, 639
FaqI GGGAC 1 cut(s) 312
FatI CATG 3 cut(s) 361, 451, 635
FauNDI CATATG 1 cut(s) 729
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 1 cut(s) 473
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 2 cut(s) 41, 176
GluI GCNGC 1 cut(s) 473
HaeIII GGCC 1 cut(s) 255
HapII CCGG 2 cut(s) 75, 158
Hin1II CATG 3 cut(s) 365, 455, 639
HinfI GANTC 2 cut(s) 185, 385
HpaII CCGG 2 cut(s) 75, 158
HphI GGTGA 1 cut(s) 300
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 2 cut(s) 564, 643
Hpy188III TCNNGA 2 cut(s) 176, 241
Hpy8I GTNNAC 1 cut(s) 38
HpyAV CCTTC 1 cut(s) 273
HpyCH4III ACNGT 3 cut(s) 317, 507, 620
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 7 cut(s) 18, 145, 163, 191, 455, 635, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 461
HpyF3I CTNAG 3 cut(s) 137, 303, 629
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 3 cut(s) 365, 455, 639
Ksp22I TGATCA 1 cut(s) 705
Kzo9I GATC 2 cut(s) 237, 705
LguI GCTCTTC 1 cut(s) 285
Lsp1109I GCAGC 1 cut(s) 484
MaeI CTAG 2 cut(s) 41, 176
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 5 cut(s) 56, 306, 437, 592, 620
MalI GATC 2 cut(s) 239, 707
MbiI CCGCTC 1 cut(s) 530
MboI GATC 2 cut(s) 237, 705
MboII GAAGA 8 cut(s) 56, 162, 302, 311, 440, 440, 510, 648
MluCI AATT 3 cut(s) 534, 688, 700
MlyI GAGTC 1 cut(s) 394
MnlI CCTC 5 cut(s) 20, 172, 325, 454, 492
Mph1103I ATGCAT 1 cut(s) 457
MslI CAYNNNNRTG 1 cut(s) 450
MspI CCGG 2 cut(s) 75, 158
MspR9I CCNGG 3 cut(s) 76, 257, 434
MvaI CCWGG 2 cut(s) 257, 434
MwoI GCNNNNNNNGC 2 cut(s) 332, 461
NciI CCSGG 1 cut(s) 76
NcoI CCATGG 1 cut(s) 361
NdeI CATATG 1 cut(s) 729
NdeII GATC 2 cut(s) 237, 705
NlaIII CATG 3 cut(s) 365, 455, 639
NlaIV GGNNCC 2 cut(s) 261, 300
NmuCI GTSAC 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 639
PaqCI CACCTGC 2 cut(s) 182, 292
PciSI GCTCTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PpuMI RGGWCCY 1 cut(s) 299
PshAI GACNNNNGTC 1 cut(s) 304
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 2 cut(s) 255, 432
PspGI CCWGG 2 cut(s) 255, 432
PspN4I GGNNCC 2 cut(s) 261, 300
PspPI GGNCC 2 cut(s) 253, 299
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 193
RsaI GTAC 3 cut(s) 80, 101, 121
RsaNI GTAC 3 cut(s) 79, 100, 120
RseI CAYNNNNRTG 1 cut(s) 450
SapI GCTCTTC 1 cut(s) 285
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 2 cut(s) 237, 705
Sau96I GGNCC 2 cut(s) 253, 299
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 76, 257, 434
SetI ASST 9 cut(s) 98, 143, 183, 196, 261, 286, 304, 313, 553
SfcI CTRYAG 1 cut(s) 189
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 450
Sse9I AATT 3 cut(s) 534, 688, 700
SsiI CCGC 1 cut(s) 530
SspI AATATT 2 cut(s) 355, 718
SspMI CTAG 2 cut(s) 41, 176
StyD4I CCNGG 3 cut(s) 74, 255, 432
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 3 cut(s) 317, 507, 620
TaiI ACGT 1 cut(s) 553
TaqI TCGA 1 cut(s) 131
TasI AATT 3 cut(s) 534, 688, 700
TatI WGTACW 1 cut(s) 119
TfiI GAWTC 1 cut(s) 185
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 4 cut(s) 255, 393, 440, 441
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 534
XbaI TCTAGA 1 cut(s) 175
XceI RCATGY 1 cut(s) 639
XspI CTAG 2 cut(s) 41, 176
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.