Rh4DG177000
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005535
Physical Location & Seq
Reverse (-)
35110429 .. 35110872
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG177000.1

Sequence Viewer

Length: 444 bp
ATGGAGTTGATGAGTGGTCGAAAAGCTGTAGATGATACCATGCCAGATGAAAGGTCTCATTTGGTCTCATGGTTTCGCAGAGTCCTTGTCAACAAAGAAAACATTCGCAACTCCATCGACCAAACTCTTGACGCTAATGAGGAGACAATGGAGAGCATATACAAAGTTGCTGAGCTTGCAGAACACTGCACAGCTCCTGAGTCATATCAGAGACCAGACATGGGCCATGCAGTCAAGATCTTGGGTCCTCTTGTTGAGCACTGGAGACCTACAACCCACGAAGAAGAAGATGAAGGTAGTTCAAGGATGTTTGATGATCTGTCCTATATGCACATGAACCTTTCTCAAGCAGCGGAAAGATGGCAAGCCGATGAAGGTACTTCAAGGATGTTTGTTGATCAGTCCTCTAGCCATACTCAATCAAGCATCCATTCAAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

147

Amino Acids

16.92

Weight (kDa)

4.9

Isoelectric Point (pI)

66.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 353
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 303, 384, 435
AluBI AGCT 3 cut(s) 26, 175, 194
AluI AGCT 3 cut(s) 26, 175, 194
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 5 cut(s) 60, 70, 137, 205, 259
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 223, 245
AvaII GGWCC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 362
BccI CCATC 2 cut(s) 122, 354
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 5 cut(s) 60, 70, 137, 205, 259
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 27
BglII AGATCT 1 cut(s) 237
BisI GCNGC 1 cut(s) 351
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 352
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 223, 245
BmiI GGNNCC 1 cut(s) 246
BmsI GCATC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 283
Bpu1102I GCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 330
BsaI GGTCTC 4 cut(s) 60, 70, 205, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 266
BseGI GGATG 3 cut(s) 312, 393, 426
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 162, 189
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 362
BsgI GTGCAG 1 cut(s) 172
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 5 cut(s) 60, 70, 137, 205, 259
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 4 cut(s) 60, 70, 205, 259
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 237, 316, 397
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 1 cut(s) 353
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 163, 190
BspLI GGNNCC 1 cut(s) 246
BspTNI GGTCTC 4 cut(s) 60, 70, 205, 259
BsrI ACTGG 1 cut(s) 266
BssMI GATC 3 cut(s) 237, 316, 397
BstC8I GCNNGC 2 cut(s) 177, 366
BstDEI CTNAG 2 cut(s) 171, 198
BstF5I GGATG 3 cut(s) 312, 393, 426
BstKTI GATC 3 cut(s) 240, 319, 400
BstMAI GTCTC 5 cut(s) 60, 70, 137, 205, 259
BstMBI GATC 3 cut(s) 237, 316, 397
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 362
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 3 cut(s) 312, 393, 426
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 184, 259
Cac8I GCNNGC 2 cut(s) 177, 366
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 223, 245
CseI GACGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 378
CviAII CATG 5 cut(s) 40, 69, 220, 227, 334
CviJI RGCY 6 cut(s) 26, 175, 194, 225, 368, 411
CviKI_1 RGCY 6 cut(s) 26, 175, 194, 225, 368, 411
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 2 cut(s) 171, 198
DpnI GATC 3 cut(s) 239, 318, 399
DpnII GATC 3 cut(s) 237, 316, 397
Eco31I GGTCTC 4 cut(s) 60, 70, 205, 259
Eco47I GGWCC 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 245
FaeI CATG 5 cut(s) 43, 72, 223, 230, 337
FatI CATG 5 cut(s) 39, 68, 219, 226, 333
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 351
FokI GGATG 3 cut(s) 319, 400, 413
Fsp4HI GCNGC 1 cut(s) 351
FspBI CTAG 1 cut(s) 408
GluI GCNGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 1 cut(s) 225
HgaI GACGC 1 cut(s) 140
Hin1II CATG 5 cut(s) 43, 72, 223, 230, 337
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 2 cut(s) 81, 200
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 210
Hpy188III TCNNGA 3 cut(s) 128, 197, 235
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 287, 368
HpyCH4V TGCA 4 cut(s) 179, 189, 230, 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 2 cut(s) 171, 198
Hsp92II CATG 5 cut(s) 43, 72, 223, 230, 337
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 3 cut(s) 237, 316, 397
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 4 cut(s) 57, 210, 228, 247
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 1 cut(s) 435
MaeI CTAG 1 cut(s) 408
MalI GATC 3 cut(s) 239, 318, 399
MboI GATC 3 cut(s) 237, 316, 397
MboII GAAGA 3 cut(s) 293, 296, 299
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 261
MlyI GAGTC 2 cut(s) 90, 209
MnlI CCTC 3 cut(s) 133, 258, 415
MroXI GAANNNNTTC 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeII GATC 3 cut(s) 237, 316, 397
NlaIII CATG 5 cut(s) 43, 72, 223, 230, 337
NlaIV GGNNCC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 352
PleI GAGTC 2 cut(s) 89, 208
PpsI GAGTC 2 cut(s) 89, 208
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 2 cut(s) 223, 245
PspPPI RGGWCCY 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
SatI GCNGC 1 cut(s) 351
Sau3AI GATC 3 cut(s) 237, 316, 397
Sau96I GGNCC 2 cut(s) 223, 245
SchI GAGTC 2 cut(s) 90, 209
SduI GDGCHC 1 cut(s) 261
SetI ASST 8 cut(s) 28, 56, 177, 196, 271, 298, 342, 379
SfaNI GCATC 1 cut(s) 435
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
SsiI CCGC 1 cut(s) 353
SspMI CTAG 1 cut(s) 408
TaqI TCGA 2 cut(s) 19, 117
TscAI CASTG 2 cut(s) 191, 266
TseI GCWGC 1 cut(s) 350
TspDTI ATGAA 4 cut(s) 63, 306, 350, 387
TspRI CASTG 2 cut(s) 191, 266
VpaK11BI GGWCC 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 102
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.