Rh5AG070200

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000985
Physical Location & Seq
Reverse (-)
5923954 .. 5928361
4408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG070200.1

Sequence Viewer

Length: 591 bp
ATGTCCTCCTTGGCCGCTAGGTCAGTGCTCCGCTCCACCGCGTCTCGAACCACCCAGCTGCGTTCGAGGCTCGGTTTCGCAGCCAAAACCAAACCAGTCGCCTCACCCTTTAGCATCCCCAAACAAGACCAGAACCTACTCTCTCATCGCATCTTCAGGTCGCCTGTGGAGTTGAGCTGCTGCGTGGAGACGTTGCTTCCCTACCATACAGCCACAGCCTCTGCATTACTCACTTCGAAGCTAGAAGTCTCGCAACGCTCCTATGTTTTGATCCCTGAAGAGCAGCTAAATCAGTGGCTATGGAACATTATAGATGAGTTAGTGTACCAGTTTCAGAGCTTCTGTCAGTATAGCGCTAAGACGAAGAACAAGACTGAGCAGAAGACTACTTTCCTCAGACAGTTTGATCAAAAGCCGCTTAACCTCAAAATGGAAGAGAGACTGAAGAAGGAAGGTTTTAACATTGCACCAAACTTGATGAATCAAGCAAAAGATTATTTGAACAAAACTATGTTGGTTATATTGTTCGCTAGATTTAAGGAAAGCAGGGGTCAATTTGCACAAGAACATATTGCCCCCAAACTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.61

Weight (kDa)

9.82

Isoelectric Point (pI)

65.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Paf67 PF10255 97 - 138 2.3e-12 RNA polymerase I-associated factor PAF67
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 33
AccII CGCG 1 cut(s) 41
AciI CCGC 4 cut(s) 15, 31, 39, 416
AclWI GGATC 1 cut(s) 265
AcoI YGGCCR 1 cut(s) 12
AcuI CTGAAG 3 cut(s) 139, 297, 464
AfaI GTAC 1 cut(s) 326
AfeI AGCGCT 1 cut(s) 355
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 1 cut(s) 502
AluBI AGCT 5 cut(s) 58, 177, 241, 286, 339
AluI AGCT 5 cut(s) 58, 177, 241, 286, 339
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 4 cut(s) 48, 182, 253, 433
AlwI GGATC 1 cut(s) 265
AlwNI CAGNNNCTG 1 cut(s) 221
Aor51HI AGCGCT 1 cut(s) 355
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 5 cut(s) 58, 80, 177, 180, 283
AspLEI GCGC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 96
AsuII TTCGAA 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 5 cut(s) 45, 92, 164, 167, 295
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 4 cut(s) 48, 182, 253, 433
BfaI CTAG 3 cut(s) 18, 242, 531
BfoI RGCGCY 1 cut(s) 357
BisI GCNGC 7 cut(s) 15, 59, 81, 178, 181, 284, 416
BlsI GCNGC 7 cut(s) 16, 60, 82, 179, 182, 285, 417
BmsI GCATC 2 cut(s) 123, 159
BpiI GAAGAC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 2 cut(s) 95, 328
Bse3DI GCAATG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 366, 409
BseNI ACTGG 2 cut(s) 95, 328
BseXI GCAGC 5 cut(s) 45, 92, 164, 167, 295
BseYI CCCAGC 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 4 cut(s) 48, 182, 253, 433
BsmBI CGTCTC 2 cut(s) 48, 182
BsnI GGCC 1 cut(s) 14
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 2 cut(s) 270, 406
BspACI CCGC 4 cut(s) 15, 31, 39, 416
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 367, 408
BspFNI CGCG 1 cut(s) 41
BspPI GGATC 1 cut(s) 265
BspQI GCTCTTC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 2 cut(s) 95, 328
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 2 cut(s) 270, 406
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 402
Bst6I CTCTTC 2 cut(s) 273, 429
BstBI TTCGAA 1 cut(s) 236
BstDEI CTNAG 3 cut(s) 357, 375, 395
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 2 cut(s) 273, 409
BstMAI GTCTC 4 cut(s) 48, 182, 253, 433
BstMBI GATC 2 cut(s) 270, 406
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 5 cut(s) 45, 92, 164, 167, 295
BstV2I GAAGAC 1 cut(s) 389
BsuRI GGCC 1 cut(s) 14
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 2 cut(s) 30, 299
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 1 cut(s) 356
CseI GACGC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 325
CviAII CATG 1 cut(s) 586
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 3 cut(s) 357, 375, 395
DpnI GATC 2 cut(s) 272, 408
DpnII GATC 2 cut(s) 270, 406
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 2 cut(s) 273, 429
EarI CTCTTC 2 cut(s) 273, 429
Eco130I CCWWGG 1 cut(s) 9
Eco47III AGCGCT 1 cut(s) 355
Eco57I CTGAAG 3 cut(s) 139, 297, 464
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 2 cut(s) 48, 182
FaeI CATG 1 cut(s) 589
FaiI YATR 9 cut(s) 207, 264, 301, 311, 351, 512, 521, 570, 587
FatI CATG 1 cut(s) 585
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 7 cut(s) 15, 59, 81, 178, 181, 284, 416
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 7 cut(s) 15, 59, 81, 178, 181, 284, 416
FspBI CTAG 3 cut(s) 18, 242, 531
GlaI GCGC 1 cut(s) 355
GluI GCNGC 7 cut(s) 15, 59, 81, 178, 181, 284, 416
GsaI CCCAGC 1 cut(s) 58
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 1 cut(s) 14
HgaI GACGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 356
Hin1II CATG 1 cut(s) 589
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 1 cut(s) 481
HphI GGTGA 1 cut(s) 96
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 2 cut(s) 336, 398
Hpy188III TCNNGA 1 cut(s) 45
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 442, 446
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 3 cut(s) 224, 467, 560
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 3 cut(s) 357, 375, 395
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 1 cut(s) 589
HspAI GCGC 1 cut(s) 354
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 2 cut(s) 270, 406
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 3 cut(s) 33, 38, 263
LpnPI CCDG 8 cut(s) 68, 108, 142, 143, 177, 288, 341, 532
Lsp1109I GCAGC 5 cut(s) 45, 92, 164, 167, 295
LweI GCATC 2 cut(s) 123, 159
MaeI CTAG 3 cut(s) 18, 242, 531
MaeII ACGT 1 cut(s) 191
MalI GATC 2 cut(s) 272, 408
MbiI CCGCTC 1 cut(s) 33
MboI GATC 2 cut(s) 270, 406
MboII GAAGA 6 cut(s) 145, 290, 376, 394, 446, 457
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 1 cut(s) 554
MnlI CCTC 6 cut(s) 16, 60, 112, 229, 404, 434
MseI TTAA 3 cut(s) 420, 459, 537
MspA1I CMGCKG 1 cut(s) 58
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 67
NdeII GATC 2 cut(s) 270, 406
NlaIII CATG 1 cut(s) 589
NspV TTCGAA 1 cut(s) 236
PciSI GCTCTTC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 481
PkrI GCNGC 7 cut(s) 16, 60, 82, 179, 182, 285, 417
PspFI CCCAGC 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 3 cut(s) 420, 459, 537
SatI GCNGC 7 cut(s) 15, 59, 81, 178, 181, 284, 416
Sau3AI GATC 2 cut(s) 270, 406
SduI GDGCHC 1 cut(s) 30
SfaNI GCATC 2 cut(s) 123, 159
SfuI TTCGAA 1 cut(s) 236
Sse9I AATT 1 cut(s) 554
SsiI CCGC 4 cut(s) 15, 31, 39, 416
SspMI CTAG 3 cut(s) 18, 242, 531
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 402
TaiI ACGT 1 cut(s) 194
TaqI TCGA 3 cut(s) 46, 65, 236
TasI AATT 1 cut(s) 554
TauI GCSGC 2 cut(s) 17, 418
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 3 cut(s) 420, 459, 537
Tru9I TTAA 3 cut(s) 420, 459, 537
TscAI CASTG 2 cut(s) 30, 299
TseI GCWGC 5 cut(s) 58, 80, 177, 180, 283
TspDTI ATGAA 1 cut(s) 494
TspRI CASTG 2 cut(s) 30, 299
XspI CTAG 3 cut(s) 18, 242, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.