Rh7BG254800

Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006383
Physical Location & Seq
Forward (+)
23224544 .. 23224819
276 bp
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UTR
Exon/CDS
Intron
Rh7BG254800.1

Sequence Viewer

Length: 276 bp
ATGACAATTCTCGTCAAGGCAGGCGCCCCAGTCGACCACACCATCGCCTCCCTTTCCCCCTACATGGAGCCCGGCGACGCCATCATCGACGGCGGCAATGAGTGGTACAAGAGCACCGAGCGCTGCGTCCACGAGGCCTCCGCCAAGGGCCTCCTCTACCTCGGCATGGGAGTCTTCGGCGACGACGAGGGCGCCCGCCACGGCCCCAGTCTCATGCCCGGTGGGTTTCACAAGGTGTATTCCAACATACGTGTTGAAGAACATCGGCGGACTTGA

Protein Analysis

91

Amino Acids

9.9

Weight (kDa)

5.89

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 3 - 83 2.7e-19 NAD binding domain of 6-phosphogluconate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 23, 191
AccI GTMKAC 1 cut(s) 33
AciI CCGC 4 cut(s) 93, 141, 196, 268
AcyI GRCGYC 3 cut(s) 24, 78, 192
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 1 cut(s) 107
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 2 cut(s) 64, 166
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 1 cut(s) 257
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 215
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 3 cut(s) 135, 148, 202
ApeKI GCWGC 1 cut(s) 123
AspLEI GCGC 3 cut(s) 26, 123, 194
AspS9I GGNCC 2 cut(s) 148, 203
AsuC2I CCSGG 2 cut(s) 72, 219
BanI GGYRCC 2 cut(s) 23, 191
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 110
BccI CCATC 2 cut(s) 50, 89
BceAI ACGGC 2 cut(s) 106, 217
BcnI CCSGG 2 cut(s) 72, 219
BcoDI GTCTC 1 cut(s) 215
BfoI RGCGCY 3 cut(s) 27, 124, 195
BisI GCNGC 2 cut(s) 94, 124
BlsI GCNGC 2 cut(s) 95, 125
Bme1390I CCNGG 2 cut(s) 72, 219
BmgT120I GGNCC 2 cut(s) 148, 203
BmiI GGNNCC 4 cut(s) 25, 69, 193, 205
BmrFI CCNGG 2 cut(s) 72, 219
BmrI ACTGGG 2 cut(s) 23, 201
BmuI ACTGGG 2 cut(s) 23, 201
BpiI GAAGAC 1 cut(s) 166
BpuMI CCSGG 2 cut(s) 72, 219
BsaAI YACGTR 1 cut(s) 251
BsaHI GRCGYC 3 cut(s) 24, 78, 192
BsaJI CCNNGG 3 cut(s) 144, 160, 199
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 166
Bse1I ACTGG 2 cut(s) 29, 207
Bse3DI GCAATG 1 cut(s) 103
BseDI CCNNGG 3 cut(s) 144, 160, 199
BseLI CCNNNNNNNGG 2 cut(s) 64, 166
BseMI GCAATG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 29, 207
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 110
BshFI GGCC 3 cut(s) 137, 150, 204
BshNI GGYRCC 2 cut(s) 23, 191
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 2 cut(s) 72, 219
BslI CCNNNNNNNGG 2 cut(s) 64, 166
BsmAI GTCTC 1 cut(s) 215
BsnI GGCC 3 cut(s) 137, 150, 204
Bsp1286I GDGCHC 2 cut(s) 72, 116
BspACI CCGC 4 cut(s) 93, 141, 196, 268
BspANI GGCC 3 cut(s) 137, 150, 204
BspLI GGNNCC 4 cut(s) 25, 69, 193, 205
BspT107I GGYRCC 2 cut(s) 23, 191
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 2 cut(s) 29, 207
BssECI CCNNGG 3 cut(s) 144, 160, 199
BssNI GRCGYC 3 cut(s) 24, 78, 192
BssSI CACGAG 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 131
BstACI GRCGYC 3 cut(s) 24, 78, 192
BstBAI YACGTR 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 22, 196
BstDSI CCRYGG 1 cut(s) 199
BstH2I RGCGCY 3 cut(s) 27, 124, 195
BstHHI GCGC 3 cut(s) 26, 123, 194
BstMAI GTCTC 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstSCI CCNGG 2 cut(s) 70, 217
BstV1I GCAGC 1 cut(s) 110
BstV2I GAAGAC 1 cut(s) 166
BsuRI GGCC 3 cut(s) 137, 150, 204
BtgI CCRYGG 1 cut(s) 199
BtgZI GCGATG 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 22, 196
CfoI GCGC 3 cut(s) 26, 123, 194
Cfr13I GGNCC 2 cut(s) 148, 203
CseI GACGC 2 cut(s) 86, 115
Csp6I GTAC 1 cut(s) 106
CviAII CATG 3 cut(s) 64, 166, 214
CviJI RGCY 4 cut(s) 70, 137, 150, 204
CviKI_1 RGCY 4 cut(s) 70, 137, 150, 204
CviQI GTAC 1 cut(s) 106
DinI GGCGCC 2 cut(s) 25, 193
DraIII CACNNNGTG 1 cut(s) 235
EciI GGCGGA 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 72
Eco47III AGCGCT 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 72
EgeI GGCGCC 2 cut(s) 25, 193
EheI GGCGCC 2 cut(s) 25, 193
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 3 cut(s) 67, 169, 217
FaiI YATR 4 cut(s) 65, 167, 215, 248
FatI CATG 3 cut(s) 63, 165, 213
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 94, 124
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 94, 124
GlaI GCGC 3 cut(s) 25, 122, 193
GluI GCNGC 2 cut(s) 94, 124
HaeII RGCGCY 3 cut(s) 27, 124, 195
HaeIII GGCC 3 cut(s) 137, 150, 204
HapII CCGG 2 cut(s) 72, 219
HgaI GACGC 2 cut(s) 86, 115
HhaI GCGC 3 cut(s) 26, 123, 194
Hin1I GRCGYC 3 cut(s) 24, 78, 192
Hin1II CATG 3 cut(s) 67, 169, 217
Hin6I GCGC 3 cut(s) 24, 121, 192
HinP1I GCGC 3 cut(s) 24, 121, 192
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 1 cut(s) 171
HpaII CCGG 2 cut(s) 72, 219
Hpy166II GTNNAC 2 cut(s) 34, 130
Hpy8I GTNNAC 2 cut(s) 34, 130
Hpy99I CGWCG 4 cut(s) 80, 92, 185, 188
HpyCH4IV ACGT 1 cut(s) 250
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 250
Hsp92I GRCGYC 3 cut(s) 24, 78, 192
Hsp92II CATG 3 cut(s) 67, 169, 217
HspAI GCGC 3 cut(s) 24, 121, 192
KasI GGCGCC 2 cut(s) 23, 191
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 5 cut(s) 6, 42, 85, 220, 232
Lsp1109I GCAGC 1 cut(s) 110
MaeII ACGT 1 cut(s) 250
MboII GAAGA 2 cut(s) 166, 269
MhlI GDGCHC 2 cut(s) 72, 116
MluCI AATT 1 cut(s) 6
Mly113I GGCGCC 2 cut(s) 24, 192
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 7 cut(s) 58, 127, 148, 161, 164, 170, 181
MspI CCGG 2 cut(s) 72, 219
MspR9I CCNGG 2 cut(s) 72, 219
MwoI GCNNNNNNNGC 1 cut(s) 120
NarI GGCGCC 2 cut(s) 24, 192
NciI CCSGG 2 cut(s) 72, 219
NlaIII CATG 3 cut(s) 67, 169, 217
NlaIV GGNNCC 4 cut(s) 25, 69, 193, 205
NmeAIII GCCGAG 1 cut(s) 141
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 183
PkrI GCNGC 2 cut(s) 95, 125
PleI GAGTC 1 cut(s) 179
PluTI GGCGCC 2 cut(s) 27, 195
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 251
PspN4I GGNNCC 4 cut(s) 25, 69, 193, 205
PspPI GGNCC 2 cut(s) 148, 203
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SalI GTCGAC 1 cut(s) 32
SatI GCNGC 2 cut(s) 94, 124
Sau96I GGNCC 2 cut(s) 148, 203
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 2 cut(s) 72, 219
SduI GDGCHC 2 cut(s) 72, 116
SetI ASST 3 cut(s) 162, 237, 253
SfoI GGCGCC 2 cut(s) 25, 193
Sse9I AATT 1 cut(s) 6
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 4 cut(s) 93, 141, 196, 268
SspDI GGCGCC 2 cut(s) 23, 191
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 70, 217
StyI CCWWGG 1 cut(s) 144
TaiI ACGT 1 cut(s) 253
TaqI TCGA 2 cut(s) 33, 87
TasI AATT 1 cut(s) 6
TauI GCSGC 1 cut(s) 96
TseI GCWGC 1 cut(s) 123
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.