Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001226

Physical Location & Seq

Genomic Coordinates Reverse (-)

34029134 .. 34032440

Gene Structure 3307 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGATCAACCGTTTCAAGTCCAAGATGGGTTCTTTGGGTTTGATCCATTTCAACTCTTGGAGTTCATCTTTCACTCGCAAATCTCCGGTTCTGGTTCCTTTTCCGGTTTCATTTGCTCAAAACCTCAGAATCCCCGAGAGAAAGTTTGGAAACTCGCCGGCCCATTTCCGTTTCTGCTCTCGTCGTTTTGAATCCGGCAAAGTAGAAACGCTCTCCGACGACGTTGATGCCAATCAGGTTTTGGATTTACCAGAAGGGAAGGTCAAGTTTACTCCTGAAATGAGGTTCATATCTGAGACGACTAAGGAGCGGGAGTCCTGTTATCGGGTCCTTGATGACAATGGGAAGCAAATTATGTGCAGCAACTATGCTGAGGTCGGCTCGGAAGTTGCTGTAAAAATGTATACAGACATGGTGATGCTTCAAACTATGGACATCATTTTCTACGAAGCACAGAGGCAAGGAAGAATTTCATTTTATCTGACTACAGTTGGTGAAGAAGCCATCAACATTGCATCTGCTGCAGCACTCACCTTTGATGACATTGTCCTTCCTCAGTACAGGGAGCCAGGAGTTTTAATATGGCGTGGTTTCACTGTACAAGAATTTGCAAACCAGTGTTTCAGTAACAAAGCTGATTACGGGAAAGGCAGGCAGATGCCAATCCATTATGGGTCTAACAAGCACAATTACTTCACTGTAGCATCAACCGTGGCTTCACAACTTCCACATGCTGTTGGTGTTGCTTATTCTCTAAAAATGGACAGAAAAGATGCATGTGTGGTCACATATGTTGGTGATGGTGGCACAAGTGAGGGTGACTTCCATGCTGCTCTAAATTTTGCGGCTGTTACGGAGGCTCCAGTTATATTCATTTGTCGGAACAACGGATGGGCTATCAGTACACCTACTTCAGATCAGTTTCGGAGTGATGGTGTTGTTGTCAAAGGTCGGGCTTATGGTATCCGAAGTATCCGAGTAGATGGGAATGATGCACTTGCCATGTATAGTGCAGTTTATGCTGCACGCGAAATGGCAATCAGTGAACAGAGACCAATCTTAATAGAGGCATTAACATATCGAGTTGGGCACCATTCCACATCAGATGATTCGACCAAGTATCGCCCAGTTGATGAGATTGAATTGTGGAAAGTGGAACGAGACCCAGTAACTCGATTTAGAAAATGGATTGAAAGAAATGGTTGGTGGAGTGGTGAGGCAGAGATAGAGGCTAGAAACAGTGCAAGAAAGCAGATATTACATGCAATCCAAGAAGCTGAAAAAGTAGAGAAGCCACCAGTTGCTGACATATTCACTGATGTATACGACTGTCTTCCTTCCAATCTTGTTGAGCAGGAGAAACGGCTAAGAGAGACAATTAAGAGACACCCCCAGGATTACCCATCTGATGTTCCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003826 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009987 GO:0016054 GO:0016491 GO:0017086 GO:0019752 GO:0031974 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0055114 GO:0070013 GO:0071704 GO:0098798 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00280 ko00640 ko01100 ko01110 ko01130 map00280 map00640 map01100 map01110 map01130

Pfam Domains

Protein Families

E1_dh

Protein Analysis

473

Amino Acids

53.55

Weight (kDa)

6.4

Isoelectric Point (pI)

47.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1_dh	PF00676	137 - 433	2.6e-94	Dehydrogenase E1 component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21400 AT1G21400 AT1G21400 AT1G21400 AT1G21400 AT1G21400 AT5G09300 AT5G09300 AT5G09300 AT5G09300
fragaria_vesca	FvH4_1g24490 FvH4_1g24490 FvH4_1g24490 FvH4_1g24490 FvH4_1g24490 FvH4_1g24490 FvH4_1g24490 FvH4_1g24490 FvH4_1g24490
malus_domestica	MD01G1005600.v1.1 MD15G1133900.v1.1
prunus_persica	Prupe.4G272100_v2.0.a1
pyrus_communis	pycom01g01240 pycom15g12070
rosa_chinensis	RchiOBHm_Chr2g0121681 RchiOBHm_Chr2g0121691 RchiOBHm_Chr2g0121741 RchiOBHm_Chr2g0121751
rosa_laevigata	RLG00000018629 RLG00000018630
rosa_multiflora	Rmu_co8210604.1_g000001 Rmu_co8500107.1_g000001 Rmu_sc0000315.1_g000007 Rmu_sc0002194.1_g000001 Rmu_sc0002194.1_g000003
rosa_roxburghii	Rroxscaffold_2G00122230 Rroxscaffold_2G00122280 Rroxscaffold_2G00122300 Rroxscaffold_2G00122320
rosa_rugosa	Rorug02G0234000 Rorug02G0234100
rosa_samantha	Rh2AG290900 Rh2AG291100 Rh2AG291300 Rh2AG291400 Rh2BG301700 Rh2BG301900 Rh2CG280500 Rh2CG280700 Rh2DG315500 Rh2DG315600 Rh2DG315800 Rh2DG315900
rosa_wichuraiana	Rw2G023330 Rw2G023350 Rw2G023360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1089
AccBSI	CCGCTC	1 cut(s)	310
AccI	GTMKAC	2 cut(s)	404, 1323
AccII	CGCG	1 cut(s)	1029
AciI	CCGC	2 cut(s)	310, 845
AclWI	GGATC	1 cut(s)	37
AcsI	RAATTY	3 cut(s)	468, 605, 838
AcuI	CTGAAG	1 cut(s)	897
AfaI	GTAC	3 cut(s)	560, 600, 904
AfiI	CCNNNNNNNGG	1 cut(s)	324
AgsI	TTSAA	6 cut(s)	16, 52, 191, 425, 1142, 1193
AjnI	CCWGG	2 cut(s)	568, 1392
AluBI	AGCT	2 cut(s)	635, 1277
AluI	AGCT	2 cut(s)	635, 1277
Alw26I	GTCTC	5 cut(s)	290, 1045, 1155, 1367, 1378
AlwI	GGATC	1 cut(s)	37
AlwNI	CAGNNNCTG	1 cut(s)	1304
Ama87I	CYCGRG	1 cut(s)	134
AoxI	GGCC	1 cut(s)	159
ApeKI	GCWGC	5 cut(s)	360, 521, 524, 830, 1022
ApoI	RAATTY	3 cut(s)	468, 605, 838
Asp700I	GAANNNNTTC	1 cut(s)	469
AspS9I	GGNCC	2 cut(s)	160, 328
AsuHPI	GGTGA	6 cut(s)	427, 506, 523, 809, 830, 1226
AvaI	CYCGRG	1 cut(s)	134
AvaII	GGWCC	1 cut(s)	328
BaeGI	GKGCMC	1 cut(s)	1092
BanI	GGYRCC	1 cut(s)	1089
BarI	GAAGNNNNNNTAC	2 cut(s)	895, 927
BbsI	GAAGAC	1 cut(s)	1325
BbvCI	CCTCAGC	1 cut(s)	372
BbvI	GCAGC	5 cut(s)	372, 508, 536, 817, 1009
BccI	CCATC	7 cut(s)	19, 512, 794, 885, 926, 977, 1411
BceAI	ACGGC	1 cut(s)	1379
BcgI	CGANNNNNNTGC	2 cut(s)	209, 243
BciT130I	CCWGG	2 cut(s)	570, 1394
BciVI	GTATCC	2 cut(s)	974, 983
BclI	TGATCA	1 cut(s)	3
BcoDI	GTCTC	5 cut(s)	290, 1045, 1155, 1367, 1378
BfaI	CTAG	1 cut(s)	1233
BfmI	CTRYAG	3 cut(s)	486, 522, 699
BfuI	GTATCC	2 cut(s)	974, 983
BisI	GCNGC	6 cut(s)	361, 522, 525, 831, 846, 1023
BlsI	GCNGC	6 cut(s)	362, 523, 526, 832, 847, 1024
Bme1390I	CCNGG	2 cut(s)	570, 1394
Bme18I	GGWCC	1 cut(s)	328
BmeT110I	CYCGRG	1 cut(s)	134
BmgT120I	GGNCC	2 cut(s)	160, 328
BmiI	GGNNCC	5 cut(s)	96, 329, 567, 861, 1091
BmrFI	CCNGG	2 cut(s)	570, 1394
BmrI	ACTGGG	2 cut(s)	1121, 1160
BmsI	GCATC	7 cut(s)	217, 408, 524, 648, 713, 763, 982
BmuI	ACTGGG	2 cut(s)	1121, 1160
BpiI	GAAGAC	1 cut(s)	1325
BplI	GAGNNNNNCTC	2 cut(s)	365, 397
BpmI	CTGGAG	1 cut(s)	846
Bpu10I	CCTNAGC	1 cut(s)	372
BsaBI	GATNNNNATC	2 cut(s)	231, 662
BsaI	GGTCTC	2 cut(s)	1045, 1155
BsaJI	CCNNGG	2 cut(s)	711, 1392
BsaWI	WCCGGW	2 cut(s)	85, 103
BsaXI	ACNNNNNCTCC	6 cut(s)	299, 329, 844, 874, 919, 949
Bsc4I	CCNNNNNNNGG	1 cut(s)	324
Bse118I	RCCGGY	1 cut(s)	157
Bse1I	ACTGG	5 cut(s)	616, 863, 1127, 1166, 1298
Bse3DI	GCAATG	1 cut(s)	510
Bse8I	GATNNNNATC	2 cut(s)	231, 662
BseBI	CCWGG	2 cut(s)	570, 1394
BseDI	CCNNGG	2 cut(s)	711, 1392
BseGI	GGATG	1 cut(s)	896
BseJI	GATNNNNATC	2 cut(s)	231, 662
BseLI	CCNNNNNNNGG	1 cut(s)	324
BseMI	GCAATG	1 cut(s)	510
BseMII	CTCAG	4 cut(s)	139, 285, 363, 569
BseNI	ACTGG	5 cut(s)	616, 863, 1127, 1166, 1298
BseSI	GKGCMC	1 cut(s)	1092
BseXI	GCAGC	5 cut(s)	372, 508, 536, 817, 1009
BsgI	GTGCAG	3 cut(s)	379, 1008, 1032
Bsh1236I	CGCG	1 cut(s)	1029
BshFI	GGCC	1 cut(s)	161
BshNI	GGYRCC	1 cut(s)	1089
BsiHKCI	CYCGRG	1 cut(s)	134
BsiSI	CCGG	4 cut(s)	86, 104, 158, 195
BslI	CCNNNNNNNGG	1 cut(s)	324
BsmAI	GTCTC	5 cut(s)	290, 1045, 1155, 1367, 1378
BsmBI	CGTCTC	1 cut(s)	290
BsnI	GGCC	1 cut(s)	161
Bso31I	GGTCTC	2 cut(s)	1045, 1155
BsoBI	CYCGRG	1 cut(s)	134
Bsp1286I	GDGCHC	1 cut(s)	1092
Bsp1407I	TGTACA	1 cut(s)	598
Bsp143I	GATC	3 cut(s)	3, 42, 916
BspACI	CCGC	2 cut(s)	310, 845
BspANI	GGCC	1 cut(s)	161
BspCNI	CTCAG	4 cut(s)	138, 286, 364, 568
BspFNI	CGCG	1 cut(s)	1029
BspLI	GGNNCC	5 cut(s)	96, 329, 567, 861, 1091
BspMAI	CTGCAG	1 cut(s)	526
BspPI	GGATC	1 cut(s)	37
BspT107I	GGYRCC	1 cut(s)	1089
BspTNI	GGTCTC	2 cut(s)	1045, 1155
BsrBI	CCGCTC	1 cut(s)	310
BsrDI	GCAATG	1 cut(s)	510
BsrFI	RCCGGY	1 cut(s)	157
BsrGI	TGTACA	1 cut(s)	598
BsrI	ACTGG	5 cut(s)	616, 863, 1127, 1166, 1298
BssAI	RCCGGY	1 cut(s)	157
BssECI	CCNNGG	2 cut(s)	711, 1392
BssMI	GATC	3 cut(s)	3, 42, 916
BssNAI	GTATAC	2 cut(s)	405, 1324
Bst1107I	GTATAC	2 cut(s)	405, 1324
Bst2UI	CCWGG	2 cut(s)	570, 1394
Bst4CI	ACNGT	7 cut(s)	11, 490, 598, 700, 712, 1241, 1331
BstAPI	GCANNNNNTGC	2 cut(s)	521, 1019
BstAUI	TGTACA	1 cut(s)	598
BstC8I	GCNNGC	3 cut(s)	159, 653, 1027
BstDEI	CTNAG	6 cut(s)	125, 294, 303, 372, 555, 1367
BstDSI	CCRYGG	1 cut(s)	711
BstF5I	GGATG	1 cut(s)	896
BstFNI	CGCG	1 cut(s)	1029
BstKTI	GATC	3 cut(s)	6, 45, 919
BstMAI	GTCTC	5 cut(s)	290, 1045, 1155, 1367, 1378
BstMBI	GATC	3 cut(s)	3, 42, 916
BstMWI	GCNNNNNNNGC	2 cut(s)	521, 1019
BstNI	CCWGG	2 cut(s)	570, 1394
BstNSI	RCATGY	3 cut(s)	734, 780, 1265
BstSCI	CCNGG	2 cut(s)	568, 1392
BstSFI	CTRYAG	3 cut(s)	486, 522, 699
BstSLI	GKGCMC	1 cut(s)	1092
BstUI	CGCG	1 cut(s)	1029
BstV1I	GCAGC	5 cut(s)	372, 508, 536, 817, 1009
BstV2I	GAAGAC	1 cut(s)	1325
BstZ17I	GTATAC	2 cut(s)	405, 1324
BsuI	GTATCC	2 cut(s)	974, 983
BsuRI	GGCC	1 cut(s)	161
BtgI	CCRYGG	1 cut(s)	711
BtsCI	GGATG	1 cut(s)	896
BtsIMutI	CAGTG	6 cut(s)	594, 623, 696, 1048, 1246, 1314
Cac8I	GCNNGC	3 cut(s)	159, 653, 1027
CaiI	CAGNNNCTG	1 cut(s)	1304
Cfr10I	RCCGGY	1 cut(s)	157
Cfr13I	GGNCC	2 cut(s)	160, 328
Csp6I	GTAC	3 cut(s)	559, 599, 903
CviAII	CATG	6 cut(s)	412, 731, 777, 827, 1003, 1262
CviQI	GTAC	3 cut(s)	559, 599, 903
DdeI	CTNAG	6 cut(s)	125, 294, 303, 372, 555, 1367
DpnI	GATC	3 cut(s)	5, 44, 918
DpnII	GATC	3 cut(s)	3, 42, 916
Eco31I	GGTCTC	2 cut(s)	1045, 1155
Eco47I	GGWCC	1 cut(s)	328
Eco57I	CTGAAG	1 cut(s)	897
Eco88I	CYCGRG	1 cut(s)	134
EcoO109I	RGGNCCY	1 cut(s)	328
EcoRII	CCWGG	2 cut(s)	568, 1392
EcoT22I	ATGCAT	1 cut(s)	778
Esp3I	CGTCTC	1 cut(s)	290
FaeI	CATG	6 cut(s)	415, 734, 780, 830, 1006, 1265
FatI	CATG	6 cut(s)	411, 730, 776, 826, 1002, 1261
FauI	CCCGC	1 cut(s)	303
FauNDI	CATATG	1 cut(s)	790
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	2 cut(s)	404, 1323
Fnu4HI	GCNGC	6 cut(s)	361, 522, 525, 831, 846, 1023
FokI	GGATG	1 cut(s)	903
Fsp4HI	GCNGC	6 cut(s)	361, 522, 525, 831, 846, 1023
FspBI	CTAG	1 cut(s)	1233
GluI	GCNGC	6 cut(s)	361, 522, 525, 831, 846, 1023
GsuI	CTGGAG	1 cut(s)	846
HaeIII	GGCC	1 cut(s)	161
HapII	CCGG	4 cut(s)	86, 104, 158, 195
Hin1II	CATG	6 cut(s)	415, 734, 780, 830, 1006, 1265
HinfI	GANTC	4 cut(s)	129, 191, 314, 1109
HpaII	CCGG	4 cut(s)	86, 104, 158, 195
HphI	GGTGA	6 cut(s)	427, 506, 523, 809, 830, 1226
Hpy166II	GTNNAC	5 cut(s)	270, 405, 905, 1046, 1324
Hpy188III	TCNNGA	1 cut(s)	275
Hpy8I	GTNNAC	5 cut(s)	270, 405, 905, 1046, 1324
Hpy99I	CGWCG	3 cut(s)	186, 221, 224
HpyAV	CCTTC	4 cut(s)	248, 253, 560, 1347
HpyCH4III	ACNGT	7 cut(s)	11, 490, 598, 700, 712, 1241, 1331
HpyCH4IV	ACGT	1 cut(s)	222
HpyF10VI	GCNNNNNNNGC	2 cut(s)	521, 1019
HpyF3I	CTNAG	6 cut(s)	125, 294, 303, 372, 555, 1367
HpySE526I	ACGT	1 cut(s)	222
Hsp92II	CATG	6 cut(s)	415, 734, 780, 830, 1006, 1265
KroI	GCCGGC	1 cut(s)	157
KroNI	GCCGGC	1 cut(s)	159
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	3 cut(s)	3, 42, 916
LmnI	GCTCC	3 cut(s)	307, 565, 865
Lsp1109I	GCAGC	5 cut(s)	372, 508, 536, 817, 1009
LweI	GCATC	7 cut(s)	217, 408, 524, 648, 713, 763, 982
MaeI	CTAG	1 cut(s)	1233
MaeII	ACGT	1 cut(s)	222
MaeIII	GTNAC	5 cut(s)	626, 784, 818, 850, 1168
MalI	GATC	3 cut(s)	5, 44, 918
MbiI	CCGCTC	1 cut(s)	310
MboI	GATC	3 cut(s)	3, 42, 916
MboII	GAAGA	3 cut(s)	477, 509, 1325
MhlI	GDGCHC	1 cut(s)	1092
MluCI	AATT	7 cut(s)	351, 468, 605, 688, 838, 1142, 1377
MlyI	GAGTC	1 cut(s)	323
MmeI	TCCRAC	2 cut(s)	240, 860
Mph1103I	ATGCAT	1 cut(s)	778
MroNI	GCCGGC	1 cut(s)	157
MroXI	GAANNNNTTC	1 cut(s)	469
MseI	TTAA	4 cut(s)	578, 1061, 1073, 1380
MslI	CAYNNNNRTG	1 cut(s)	416
MspI	CCGG	4 cut(s)	86, 104, 158, 195
MspR9I	CCNGG	2 cut(s)	570, 1394
MvaI	CCWGG	2 cut(s)	570, 1394
MvnI	CGCG	1 cut(s)	1029
MwoI	GCNNNNNNNGC	2 cut(s)	521, 1019
NaeI	GCCGGC	1 cut(s)	159
NdeI	CATATG	1 cut(s)	790
NdeII	GATC	3 cut(s)	3, 42, 916
NgoMIV	GCCGGC	1 cut(s)	157
NlaIII	CATG	6 cut(s)	415, 734, 780, 830, 1006, 1265
NlaIV	GGNNCC	5 cut(s)	96, 329, 567, 861, 1091
NmuCI	GTSAC	2 cut(s)	784, 818
NsiI	ATGCAT	1 cut(s)	778
NspI	RCATGY	3 cut(s)	734, 780, 1265
PdiI	GCCGGC	1 cut(s)	159
PdmI	GAANNNNTTC	1 cut(s)	469
PfeI	GAWTC	3 cut(s)	129, 191, 1109
PflFI	GACNNNGTC	1 cut(s)	545
PkrI	GCNGC	6 cut(s)	362, 523, 526, 832, 847, 1024
PleI	GAGTC	1 cut(s)	322
PpsI	GAGTC	1 cut(s)	322
PpuMI	RGGWCCY	1 cut(s)	328
Psp5II	RGGWCCY	1 cut(s)	328
Psp6I	CCWGG	2 cut(s)	568, 1392
PspGI	CCWGG	2 cut(s)	568, 1392
PspN4I	GGNNCC	5 cut(s)	96, 329, 567, 861, 1091
PspPI	GGNCC	2 cut(s)	160, 328
PspPPI	RGGWCCY	1 cut(s)	328
PstI	CTGCAG	1 cut(s)	526
PstNI	CAGNNNCTG	1 cut(s)	1304
PsyI	GACNNNGTC	1 cut(s)	545
RsaI	GTAC	3 cut(s)	560, 600, 904
RsaNI	GTAC	3 cut(s)	559, 599, 903
RseI	CAYNNNNRTG	1 cut(s)	416
SaqAI	TTAA	4 cut(s)	578, 1061, 1073, 1380
SatI	GCNGC	6 cut(s)	361, 522, 525, 831, 846, 1023
Sau3AI	GATC	3 cut(s)	3, 42, 916
Sau96I	GGNCC	2 cut(s)	160, 328
SchI	GAGTC	1 cut(s)	323
ScrFI	CCNGG	2 cut(s)	570, 1394
SduI	GDGCHC	1 cut(s)	1092
SfaNI	GCATC	7 cut(s)	217, 408, 524, 648, 713, 763, 982
SfcI	CTRYAG	3 cut(s)	486, 522, 699
SinI	GGWCC	1 cut(s)	328
SmiMI	CAYNNNNRTG	1 cut(s)	416
Sse9I	AATT	7 cut(s)	351, 468, 605, 688, 838, 1142, 1377
SsiI	CCGC	2 cut(s)	310, 845
SspMI	CTAG	1 cut(s)	1233
StyD4I	CCNGG	2 cut(s)	568, 1392
TaaI	ACNGT	7 cut(s)	11, 490, 598, 700, 712, 1241, 1331
TaiI	ACGT	1 cut(s)	225
TaqI	TCGA	3 cut(s)	1081, 1112, 1174
TasI	AATT	7 cut(s)	351, 468, 605, 688, 838, 1142, 1377
TatI	WGTACW	3 cut(s)	558, 598, 902
TauI	GCSGC	1 cut(s)	848
TfiI	GAWTC	3 cut(s)	129, 191, 1109
Tru1I	TTAA	4 cut(s)	578, 1061, 1073, 1380
Tru9I	TTAA	4 cut(s)	578, 1061, 1073, 1380
TscAI	CASTG	6 cut(s)	601, 623, 703, 1048, 1246, 1321
TseFI	GTSAC	2 cut(s)	784, 818
TseI	GCWGC	5 cut(s)	360, 521, 524, 830, 1022
Tsp45I	GTSAC	2 cut(s)	784, 818
TspDTI	ATGAA	5 cut(s)	54, 99, 277, 462, 862
TspGWI	ACGGA	3 cut(s)	158, 869, 903
TspRI	CASTG	6 cut(s)	601, 623, 703, 1048, 1246, 1321
Tth111I	GACNNNGTC	1 cut(s)	545
VpaK11BI	GGWCC	1 cut(s)	328
XapI	RAATTY	3 cut(s)	468, 605, 838
XceI	RCATGY	3 cut(s)	734, 780, 1265
XcmI	CCANNNNNNNNNTGG	1 cut(s)	238
XmiI	GTMKAC	2 cut(s)	404, 1323
XmnI	GAANNNNTTC	1 cut(s)	469
XspI	CTAG	1 cut(s)	1233
Zsp2I	ATGCAT	1 cut(s)	778

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.