Rw6G017980

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006409
Physical Location & Seq
Forward (+)
35561591 .. 35566824
5234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017980.1

Sequence Viewer

Length: 3186 bp
ATGATGAAGAAGAGATCTCTTTTTATAGTGGCGAATTATTTGGGAGGTCCAGTATTTGGCAGGGTATTTGAAATTAAATTGGATGAGTTGGATCATCGACAAGGAGGAGGAAAGGATGAGACTCCAACATTTAAATATGCAGCACAGTTATATCAAGGAGGAGAAGAGTTGAGGAAACCTATTCTATGGAAATTTTGTTCCTTGGCTACTGCCACTTCTTGCACTTCTACCAACTTGTGTGTATTGCCAGATAGTGGTCCTGAAGACCTTATGATGGATGACCCTTTGAATGGATATATCTTCAACATCAGTACTCGAAAGGTGCACAAAATGAAGCCCCCCAATGCTCCCAAGCTCTTTGGGCATGTTCTATCTTTAAATGGCAATATTTATTTTATTGCCGATCCATACTGGATAAGGGATGCACCAGATCCATCTTTCGAGCGATACGATCCTATCAAAGACTCTTGGGAAAGTTTGACACCTCTTCCCTTTAATAAGGGAATGAGTTGCGATGCTGAGATGGTGGGTTATGCTGTTTTTGATGATTTCATTTTGTTCAACATTAGTGGCCGTACCTATTCTCCTCAACTATGGGCTTATGATGTAGGTAGAAACAAATGGTACTTAGTCAGCGTCGACAACTTTGTTTGCTATGGTTTACAAGGACTGGCTGTGGTTCTGGAAAAGACTGTTTATGCCTTATCAAGCACGTCGAGTTGCTCAGTTTTGGCGTTATCTATTACAAGAGAGCAAATTGATGGACGAGATCTTGTATTTACCTGTTCTCTACAGTCGGTGTATACGTTGCCGTACACGGAGGTACTTCCATTGGATGGAGACCGGGGAAGAGACCATTTGGTTCATTTGGGTAACCGCGAGTTTTGTCTTGTCCAGAGTGGTGTCTACTATGGTTATATTGATTATCAACCTATAAGACTTAGCATTTTTCAGCTTCTGGGAAAAAACTTAGTGAATATAACATATACAATTGACTATGAGGTGCCTCTTCAGGATTTTGAGCCATTCCATGTCAGACTCTGCTTCTCCTCCCCTGGTTGCGAGGATAGTGTGTCAGAACAAGAAGATGATTCTGATATTGACTTCATCTTCGACCCGGCATTTGAAGTTGTCAAACCAGCCCCAGAAGAAGGGATCGCGACCCTGTTTGAAGAAGATGAAGAAGAAGAAGAAACAATAGTTGATACCAGTTCCTTCAAGCAGGCATCGGAAGTTGTCAAACCAGCCCCAGAAGAAGAGTTTGCGACGCTGTCTAAAGAAGAAGAAAAAATAGTTGATACCAGTTCTGTCAAGCAGTTATTAATGGCCTCTGCATCATCGGTTCCTTCTCGATGGACGTACGACGTGTTTTTGAGTTTCCGTGGTGAAGATACTCGCAAGAGTTTCATATCCCATCTATACCATGAGTTGCACATGCAAAAGATTGAAACATTCAGGGATGATCGATCACTTGAAACAGGGAGAACTCTTTCAGAGCTCTTCAGGGCAATTGAAGAATCAAGATTTGCAATTGTTGTTTTCTCGCAGAACTATGCTTTTTCCCGATGGTGCTTGATGGAAATTGCAAAGATTGTTAACGTCAACACCACTACAGTAGTGCCAATCTTTTATCATGTGGATCCTGCTGATGTACGATATCAAAGAGGGCGTTTTGCTGAAGCCTTCACTGAGCATGACAAAAGGCATGGTACTAAAGATGTGCAGGAGTGGAGAAAAGCTTTAACAAGAGTGGCAAATCTCTCTGGCTGGGATTCAAAGAATTATGTTTCCGAAAGTGAACTTATTGAACACATCATCAAATATGTGCGTAGTGAAGTGCCTCAAAAGATCACACTGTTAGATGCCAGAGAGAAGTTTGTTGCTGGGACAGTTCCTTCATCGACTTTGGAGGTTTCCTCACAAAACAATTGCCAGGAAAGCCAAAATAATGAAGGGAATAGTAGCTCAGTGGAGAGCATCAATAAAGCCGATGTTCTGGAAGAACTTTCTCTTGGTTTTCATTCAATTGCCACCAAATTTTCAGGAATTCACAGCCTAATGGAGAGAAACAGTAGTACTATATGGGATGCTATCAAGGAGGTCCCACATTTGGATATCCGGGATCGTTACCAGGTGATTGAGATACTTAATACTAAATCAAGAATAAATATGTTCTTAAGCATGTCACCTGAAGAGCGCTTGGAATGGATAACCTGCATTAGAGGCCCACCTCAGTGGAAGCATGATGTGTTTTTGACTTTTATGGCTGAAGACACCCGCGGTTTTGTATCCCATTTATGCCACGAACTGCAACATGTGCAACAGATGAAAATATTCAAGAATGATCAAGAGAAAAGAGGTACAGAGATTGCTCCAGAGCATTTGGTGGCAATTGAAAGTTCAAGGTTTGCAATAGTTGTTCTCTCGCCAAACTATGCTTCTTCCACATGGTGCTTGGATGAACTTTTAAAGATTTTAGAATGCAGAATTTCTTTGGGCACCACAGTTGTACCCATTTTTTTTCATGTGGATCCCGGGGATGTACGAAGGCTAAGAGGGAGTTTCTCAGATGCCTTTACAAAGCATGAAAAAACGCATAAGCCCTACTTAGAGCAGGTGGAAAAATGGAGACATTGTTTAAGAGAAGTGTCTAATATCTCTGGGTGGAAGTTGGACAAATATGAGAGTGAACGAGAACTTATTGAACACGTTGTTTCTGAGGTTTTCTCAGAAATCACCAGCCAAAAAAAAAGAAAAACTGAGTATGAAGGGAATAGTCACTCACTTGAGACCATCACTCAAACAGGTACTCTGGAAAGATTTGCTCTTAGCATTAAATCTATTGCTAGTGACGTTCAGCGAATCCTTAATCTAATGGAGAAAAGGAAAAGAGAAAAAGAGAGTAGTATATGGGATGCTATCATGGAGACCCCAAACTTGGATAGACTTGTTCGTTTTAAGGCTATTGATTGCCTTGATACCAAAGAAAAAAGAACATGTCACCGGAAGAGCGCTCAAACTGGATATTATACAAGTTGGGATAAGTATGGTTATTCTGACGTGATACACTCTCGTTGGACCAATGATGATACGAATTCTGAGATGATTGCGACACCCTATACACTTCGATTGCAGGAACTTTACTATGATTCGACTAAGGCTAAGAAATGTGAGACAGGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1061

Amino Acids

121.88

Weight (kDa)

5.35

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 58 - 298 8.4e-06 Protein of unknown function (DUF1668)
TIR PF01582 454 - 616 1.2e-49 TIR domain
TIR_2 PF13676 456 - 549 6.1e-13 TIR domain
At2g29880_C PF24769 694 - 739 1.7e-17 At2g29880 C-terminal domain
TIR PF01582 747 - 921 1.4e-44 TIR domain
TIR_2 PF13676 750 - 846 9.7e-11 TIR domain
At2g29880_C PF24769 970 - 998 1.2e-08 At2g29880 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2605
Acc36I ACCTGC 2 cut(s) 2222, 2605
AccB1I GGYRCC 2 cut(s) 1003, 2498
AccB7I CCANNNNNTGG 3 cut(s) 56, 254, 836
AccI GTMKAC 3 cut(s) 639, 803, 906
AccII CGCG 3 cut(s) 879, 1160, 2280
AciI CCGC 3 cut(s) 877, 2278, 2280
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 6 cut(s) 191, 2036, 2046, 2487, 3094, 3180
AcuI CTGAAG 6 cut(s) 282, 995, 1486, 1698, 2211, 2289
AdeI CACNNNGTG 1 cut(s) 2451
AfeI AGCGCT 2 cut(s) 2198, 3013
AfiI CCNNNNNNNGG 8 cut(s) 56, 254, 274, 290, 836, 1151, 2110, 2938
AflII CTTAAG 1 cut(s) 2176
AflIII ACRYGT 4 cut(s) 1365, 2314, 2707, 2996
AjiI CACGTC 3 cut(s) 714, 1366, 3061
AjnI CCWGG 3 cut(s) 1054, 1932, 2130
AjuI GAANNNNNNNTTGG 4 cut(s) 1995, 2027, 2422, 2454
AleI CACNNNNGTG 1 cut(s) 2233
AloI GAACNNNNNNTCC 2 cut(s) 181, 213
AluBI AGCT 5 cut(s) 355, 955, 1498, 1739, 1965
AluI AGCT 5 cut(s) 355, 955, 1498, 1739, 1965
Alw21I GWGCWC 2 cut(s) 327, 1500
Alw26I GTCTC 7 cut(s) 113, 834, 846, 2623, 2783, 2921, 3167
Alw44I GTGCAC 1 cut(s) 323
AlwNI CAGNNNCTG 2 cut(s) 958, 1041
Ama87I CYCGRG 1 cut(s) 2534
Aor51HI AGCGCT 2 cut(s) 2198, 3013
AoxI GGCC 3 cut(s) 571, 1326, 2224
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 6 cut(s) 191, 2036, 2046, 2487, 3094, 3180
ArsI GACNNNNNNTTYG 4 cut(s) 1094, 1106, 1126, 1138
AseI ATTAAT 1 cut(s) 1322
Asp700I GAANNNNTTC 2 cut(s) 1489, 2333
AspLEI GCGC 2 cut(s) 2199, 3014
AspS9I GGNCC 5 cut(s) 47, 257, 2101, 2225, 3078
AsuC2I CCSGG 5 cut(s) 845, 1118, 2120, 2535, 2536
AsuHPI GGTGA 5 cut(s) 1397, 2146, 2178, 2728, 2993
AvaI CYCGRG 1 cut(s) 2534
AvaII GGWCC 4 cut(s) 47, 257, 2101, 3078
BaeGI GKGCMC 2 cut(s) 327, 2501
BaeI ACNNNNGTAYC 2 cut(s) 2493, 2526
BamHI GGATCC 2 cut(s) 1639, 2530
BanI GGYRCC 2 cut(s) 1003, 2498
BanII GRGCYC 1 cut(s) 1500
BbsI GAAGAC 2 cut(s) 270, 2277
Bbv12I GWGCWC 2 cut(s) 327, 1500
BbvI GCAGC 1 cut(s) 152
BceAI ACGGC 2 cut(s) 558, 796
BciT130I CCWGG 3 cut(s) 1056, 1934, 2132
BciVI GTATCC 1 cut(s) 2299
BclI TGATCA 1 cut(s) 2344
BcnI CCSGG 5 cut(s) 845, 1118, 2120, 2535, 2536
BcoDI GTCTC 7 cut(s) 113, 834, 846, 2623, 2783, 2921, 3167
BfaI CTAG 1 cut(s) 2847
BfmI CTRYAG 2 cut(s) 791, 1611
BfoI RGCGCY 2 cut(s) 2200, 3015
BfrI CTTAAG 1 cut(s) 2176
BfuAI ACCTGC 2 cut(s) 2222, 2605
BfuI GTATCC 1 cut(s) 2299
BglII AGATCT 2 cut(s) 14, 769
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmcAI AGTACT 2 cut(s) 313, 2077
Bme1390I CCNGG 8 cut(s) 845, 1056, 1118, 1934, 2120, 2132, 2535, 2536
Bme18I GGWCC 4 cut(s) 47, 257, 2101, 3078
BmeT110I CYCGRG 1 cut(s) 2534
BmgBI CACGTC 3 cut(s) 714, 1366, 3061
BmgT120I GGNCC 5 cut(s) 47, 257, 2101, 2225, 3078
BmiI GGNNCC 6 cut(s) 1005, 1344, 1641, 2103, 2500, 2532
BmrFI CCNGG 8 cut(s) 845, 1056, 1118, 1934, 2120, 2132, 2535, 2536
BmsI GCATC 9 cut(s) 412, 505, 1235, 1343, 1852, 1986, 2077, 2560, 2905
BpiI GAAGAC 2 cut(s) 270, 2277
BplI GAGNNNNNCTC 4 cut(s) 1901, 1933, 2711, 2743
BpmI CTGGAG 1 cut(s) 2358
BpuEI CTTGAG 1 cut(s) 2807
BpuMI CCSGG 5 cut(s) 845, 1118, 2120, 2535, 2536
Bsa29I ATCGAT 1 cut(s) 1465
BsaI GGTCTC 4 cut(s) 834, 846, 2783, 2921
BsaJI CCNNGG 7 cut(s) 201, 844, 1054, 1381, 2278, 2534, 2535
BsaWI WCCGGW 1 cut(s) 3003
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 568, 598, 2620, 2650
Bsc4I CCNNNNNNNGG 8 cut(s) 56, 254, 274, 290, 836, 1151, 2110, 2938
Bse1I ACTGG 6 cut(s) 50, 416, 675, 1209, 1302, 3025
BseBI CCWGG 3 cut(s) 1056, 1934, 2132
BseCI ATCGAT 1 cut(s) 1465
BseDI CCNNGG 7 cut(s) 201, 844, 1054, 1381, 2278, 2534, 2535
BseLI CCNNNNNNNGG 8 cut(s) 56, 254, 274, 290, 836, 1151, 2110, 2938
BseNI ACTGG 6 cut(s) 50, 416, 675, 1209, 1302, 3025
BseRI GAGGAG 4 cut(s) 120, 174, 576, 1039
BseSI GKGCMC 2 cut(s) 327, 2501
BseXI GCAGC 1 cut(s) 152
BseYI CCCAGC 2 cut(s) 1767, 1883
BsgI GTGCAG 1 cut(s) 1742
Bsh1236I CGCG 3 cut(s) 879, 1160, 2280
BshFI GGCC 3 cut(s) 573, 1328, 2226
BshNI GGYRCC 2 cut(s) 1003, 2498
BshVI ATCGAT 1 cut(s) 1465
BsiHKAI GWGCWC 2 cut(s) 327, 1500
BsiHKCI CYCGRG 1 cut(s) 2534
BsiSI CCGG 5 cut(s) 844, 1118, 2119, 2535, 3004
BsiWI CGTACG 1 cut(s) 1359
BslFI GGGAC 2 cut(s) 1900, 2087
BslI CCNNNNNNNGG 8 cut(s) 56, 254, 274, 290, 836, 1151, 2110, 2938
BsmAI GTCTC 7 cut(s) 113, 834, 846, 2623, 2783, 2921, 3167
BsmFI GGGAC 2 cut(s) 1900, 2087
BsmI GAATGC 1 cut(s) 2486
BsnI GGCC 3 cut(s) 573, 1328, 2226
Bso31I GGTCTC 4 cut(s) 834, 846, 2783, 2921
BsoBI CYCGRG 1 cut(s) 2534
Bsp1286I GDGCHC 3 cut(s) 327, 1500, 2501
Bsp68I TCGCGA 1 cut(s) 1160
BspACI CCGC 3 cut(s) 877, 2278, 2280
BspANI GGCC 3 cut(s) 573, 1328, 2226
BspDI ATCGAT 1 cut(s) 1465
BspFNI CGCG 3 cut(s) 879, 1160, 2280
BspLI GGNNCC 6 cut(s) 1005, 1344, 1641, 2103, 2500, 2532
BspMI ACCTGC 2 cut(s) 2222, 2605
BspQI GCTCTTC 3 cut(s) 1505, 2187, 3002
BspT107I GGYRCC 2 cut(s) 1003, 2498
BspTI CTTAAG 1 cut(s) 2176
BspTNI GGTCTC 4 cut(s) 834, 846, 2783, 2921
BsrI ACTGG 6 cut(s) 50, 416, 675, 1209, 1302, 3025
BssECI CCNNGG 7 cut(s) 201, 844, 1054, 1381, 2278, 2534, 2535
BssNAI GTATAC 1 cut(s) 804
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 804
Bst2UI CCWGG 3 cut(s) 1056, 1934, 2132
Bst4CI ACNGT 8 cut(s) 147, 694, 795, 1615, 1857, 1891, 2072, 2506
Bst6I CTCTTC 9 cut(s) 5, 159, 492, 844, 1014, 1251, 1505, 2187, 3002
BstAFI CTTAAG 1 cut(s) 2176
BstAPI GCANNNNNTGC 1 cut(s) 2317
BstC8I GCNNGC 1 cut(s) 1224
BstDSI CCRYGG 2 cut(s) 1381, 2278
BstEII GGTNACC 1 cut(s) 872
BstFNI CGCG 3 cut(s) 879, 1160, 2280
BstH2I RGCGCY 2 cut(s) 2200, 3015
BstHHI GCGC 2 cut(s) 2199, 3014
BstMAI GTCTC 7 cut(s) 113, 834, 846, 2623, 2783, 2921, 3167
BstMWI GCNNNNNNNGC 4 cut(s) 361, 1938, 2223, 2317
BstNI CCWGG 3 cut(s) 1056, 1934, 2132
BstNSI RCATGY 5 cut(s) 368, 1438, 2185, 2318, 3000
BstPI GGTNACC 1 cut(s) 872
BstSCI CCNGG 8 cut(s) 843, 1054, 1116, 1932, 2118, 2130, 2533, 2534
BstSFI CTRYAG 2 cut(s) 791, 1611
BstSLI GKGCMC 2 cut(s) 327, 2501
BstUI CGCG 3 cut(s) 879, 1160, 2280
BstV1I GCAGC 1 cut(s) 152
BstV2I GAAGAC 2 cut(s) 270, 2277
BstX2I RGATCY 5 cut(s) 14, 430, 769, 1639, 2530
BstXI CCANNNNNNTGG 1 cut(s) 2235
BstYI RGATCY 5 cut(s) 14, 430, 769, 1639, 2530
BstZ17I GTATAC 1 cut(s) 804
Bsu15I ATCGAT 1 cut(s) 1465
BsuI GTATCC 1 cut(s) 2299
BsuRI GGCC 3 cut(s) 573, 1328, 2226
BsuTUI ATCGAT 1 cut(s) 1465
BtgI CCRYGG 2 cut(s) 1381, 2278
BtgZI GCGATG 1 cut(s) 528
BtrI CACGTC 3 cut(s) 714, 1366, 3061
BtsIMutI CAGTG 4 cut(s) 1686, 1853, 1974, 2240
BtuMI TCGCGA 1 cut(s) 1160
BveI ACCTGC 2 cut(s) 2222, 2605
Cac8I GCNNGC 1 cut(s) 1224
CaiI CAGNNNCTG 2 cut(s) 958, 1041
CfoI GCGC 2 cut(s) 2199, 3014
Cfr13I GGNCC 5 cut(s) 47, 257, 2101, 2225, 3078
Cfr42I CCGCGG 1 cut(s) 2281
Cfr9I CCCGGG 1 cut(s) 2534
ClaI ATCGAT 1 cut(s) 1465
CseI GACGC 2 cut(s) 625, 1276
CsiI ACCWGGT 1 cut(s) 2130
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
DraI TTTAAA 3 cut(s) 133, 378, 2469
DraIII CACNNNGTG 1 cut(s) 2451
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 9 cut(s) 5, 159, 492, 844, 1014, 1251, 1505, 2187, 3002
EarI CTCTTC 9 cut(s) 5, 159, 492, 844, 1014, 1251, 1505, 2187, 3002
Ecl136II GAGCTC 1 cut(s) 1498
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 1500
Eco31I GGTCTC 4 cut(s) 834, 846, 2783, 2921
Eco32I GATATC 2 cut(s) 1658, 2116
Eco47I GGWCC 4 cut(s) 47, 257, 2101, 3078
Eco47III AGCGCT 2 cut(s) 2198, 3013
Eco53kI GAGCTC 1 cut(s) 1498
Eco57I CTGAAG 6 cut(s) 282, 995, 1486, 1698, 2211, 2289
Eco88I CYCGRG 1 cut(s) 2534
Eco91I GGTNACC 1 cut(s) 872
EcoICRI GAGCTC 1 cut(s) 1498
EcoO109I RGGNCCY 1 cut(s) 2101
EcoO65I GGTNACC 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 2046, 3094
EcoRII CCWGG 3 cut(s) 1054, 1932, 2130
EcoRV GATATC 2 cut(s) 1658, 2116
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 1500
ErhI CCWWGG 1 cut(s) 201
FalI AAGNNNNNCTT 2 cut(s) 2591, 2623
FaqI GGGAC 2 cut(s) 1900, 2087
FauI CCCGC 1 cut(s) 2285
FbaI TGATCA 1 cut(s) 2344
FblI GTMKAC 3 cut(s) 639, 803, 906
Fnu4HI GCNGC 1 cut(s) 141
FriOI GRGCYC 1 cut(s) 1500
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 2847
GlaI GCGC 2 cut(s) 2198, 3013
GluI GCNGC 1 cut(s) 141
GsaI CCCAGC 2 cut(s) 1771, 1887
GsuI CTGGAG 1 cut(s) 2358
HaeII RGCGCY 2 cut(s) 2200, 3015
HaeIII GGCC 3 cut(s) 573, 1328, 2226
HapII CCGG 5 cut(s) 844, 1118, 2119, 2535, 3004
HgaI GACGC 2 cut(s) 625, 1276
HhaI GCGC 2 cut(s) 2199, 3014
Hin6I GCGC 2 cut(s) 2197, 3012
HinP1I GCGC 2 cut(s) 2197, 3012
HincII GTYRAC 3 cut(s) 640, 1597, 1603
HindII GTYRAC 3 cut(s) 640, 1597, 1603
HindIII AAGCTT 1 cut(s) 1737
HinfI GANTC 8 cut(s) 121, 464, 1038, 1091, 1517, 1772, 2862, 3149
HpaI GTTAAC 1 cut(s) 1597
HpaII CCGG 5 cut(s) 844, 1118, 2119, 2535, 3004
HphI GGTGA 5 cut(s) 1397, 2146, 2178, 2728, 2993
Hpy99I CGWCG 4 cut(s) 641, 718, 1270, 1367
HpyAV CCTTC 8 cut(s) 1145, 1225, 1356, 1693, 1905, 1946, 2541, 2762
HpyCH4III ACNGT 8 cut(s) 147, 694, 795, 1615, 1857, 1891, 2072, 2506
HpyCH4IV ACGT 8 cut(s) 713, 806, 1358, 1365, 1599, 2709, 2853, 3060
HpyF10VI GCNNNNNNNGC 4 cut(s) 361, 1938, 2223, 2317
HpySE526I ACGT 8 cut(s) 713, 806, 1358, 1365, 1599, 2709, 2853, 3060
HspAI GCGC 2 cut(s) 2197, 3012
Ksp22I TGATCA 1 cut(s) 2344
KspAI GTTAAC 1 cut(s) 1597
KspI CCGCGG 1 cut(s) 2281
LguI GCTCTTC 3 cut(s) 1505, 2187, 3002
LmnI GCTCC 2 cut(s) 352, 2377
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 9 cut(s) 412, 505, 1235, 1343, 1852, 1986, 2077, 2560, 2905
MabI ACCWGGT 1 cut(s) 2130
MaeI CTAG 1 cut(s) 2847
MaeII ACGT 8 cut(s) 713, 806, 1358, 1365, 1599, 2709, 2853, 3060
MaeIII GTNAC 6 cut(s) 872, 2126, 2184, 2777, 2849, 2999
MfeI CAATTG 6 cut(s) 990, 1509, 1530, 1927, 2025, 2391
MflI RGATCY 5 cut(s) 14, 430, 769, 1639, 2530
MhlI GDGCHC 3 cut(s) 327, 1500, 2501
MlyI GAGTC 3 cut(s) 115, 458, 1032
MmeI TCCRAC 4 cut(s) 69, 149, 2652, 3056
MroXI GAANNNNTTC 2 cut(s) 1489, 2333
MslI CAYNNNNRTG 1 cut(s) 2233
MspA1I CMGCKG 1 cut(s) 2280
MspCI CTTAAG 1 cut(s) 2176
MspI CCGG 5 cut(s) 844, 1118, 2119, 2535, 3004
MspR9I CCNGG 8 cut(s) 845, 1056, 1118, 1934, 2120, 2132, 2535, 2536
MunI CAATTG 6 cut(s) 990, 1509, 1530, 1927, 2025, 2391
Mva1269I GAATGC 1 cut(s) 2486
MvaI CCWGG 3 cut(s) 1056, 1934, 2132
MvnI CGCG 3 cut(s) 879, 1160, 2280
MwoI GCNNNNNNNGC 4 cut(s) 361, 1938, 2223, 2317
NciI CCSGG 5 cut(s) 845, 1118, 2120, 2535, 2536
NlaIV GGNNCC 6 cut(s) 1005, 1344, 1641, 2103, 2500, 2532
NmuCI GTSAC 4 cut(s) 2184, 2777, 2849, 2999
NruI TCGCGA 1 cut(s) 1160
NspI RCATGY 5 cut(s) 368, 1438, 2185, 2318, 3000
OliI CACNNNNGTG 1 cut(s) 2233
PaqCI CACCTGC 1 cut(s) 2605
PciI ACATGT 2 cut(s) 2314, 2996
PciSI GCTCTTC 3 cut(s) 1505, 2187, 3002
PcsI WCGNNNNNNNCGW 2 cut(s) 447, 803
PctI GAATGC 1 cut(s) 2486
PdmI GAANNNNTTC 2 cut(s) 1489, 2333
PfeI GAWTC 5 cut(s) 1091, 1517, 1772, 2862, 3149
Pfl23II CGTACG 1 cut(s) 1359
PflFI GACNNNGTC 1 cut(s) 1270
PflMI CCANNNNNTGG 3 cut(s) 56, 254, 836
PfoI TCCNGGA 1 cut(s) 2118
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 3 cut(s) 115, 458, 1032
PpsI GAGTC 3 cut(s) 115, 458, 1032
PpuMI RGGWCCY 1 cut(s) 2101
PscI ACATGT 2 cut(s) 2314, 2996
PshBI ATTAAT 1 cut(s) 1322
Psp124BI GAGCTC 1 cut(s) 1500
Psp5II RGGWCCY 1 cut(s) 2101
Psp6I CCWGG 3 cut(s) 1054, 1932, 2130
PspEI GGTNACC 1 cut(s) 872
PspFI CCCAGC 2 cut(s) 1767, 1883
PspGI CCWGG 3 cut(s) 1054, 1932, 2130
PspLI CGTACG 1 cut(s) 1359
PspN4I GGNNCC 6 cut(s) 1005, 1344, 1641, 2103, 2500, 2532
PspPI GGNCC 5 cut(s) 47, 257, 2101, 2225, 3078
PspPPI RGGWCCY 1 cut(s) 2101
PstNI CAGNNNCTG 2 cut(s) 958, 1041
PsuI RGATCY 5 cut(s) 14, 430, 769, 1639, 2530
PsyI GACNNNGTC 1 cut(s) 1270
RruI TCGCGA 1 cut(s) 1160
RseI CAYNNNNRTG 1 cut(s) 2233
SacI GAGCTC 1 cut(s) 1500
SacII CCGCGG 1 cut(s) 2281
SalI GTCGAC 1 cut(s) 638
SapI GCTCTTC 3 cut(s) 1505, 2187, 3002
SatI GCNGC 1 cut(s) 141
Sau96I GGNCC 5 cut(s) 47, 257, 2101, 2225, 3078
ScaI AGTACT 2 cut(s) 313, 2077
SchI GAGTC 3 cut(s) 115, 458, 1032
ScrFI CCNGG 8 cut(s) 845, 1056, 1118, 1934, 2120, 2132, 2535, 2536
SduI GDGCHC 3 cut(s) 327, 1500, 2501
SexAI ACCWGGT 1 cut(s) 2130
SfaNI GCATC 9 cut(s) 412, 505, 1235, 1343, 1852, 1986, 2077, 2560, 2905
SfcI CTRYAG 2 cut(s) 791, 1611
Sfr303I CCGCGG 1 cut(s) 2281
SgrBI CCGCGG 1 cut(s) 2281
SinI GGWCC 4 cut(s) 47, 257, 2101, 3078
SmaI CCCGGG 1 cut(s) 2536
SmiI ATTTAAAT 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 2233
SmlI CTYRAG 2 cut(s) 2176, 2786
SmoI CTYRAG 2 cut(s) 2176, 2786
SsiI CCGC 3 cut(s) 877, 2278, 2280
SspI AATATT 2 cut(s) 388, 2334
SspMI CTAG 1 cut(s) 2847
SstI GAGCTC 1 cut(s) 1500
StyD4I CCNGG 8 cut(s) 843, 1054, 1116, 1932, 2118, 2130, 2533, 2534
StyI CCWWGG 1 cut(s) 201
SwaI ATTTAAAT 1 cut(s) 133
TaaI ACNGT 8 cut(s) 147, 694, 795, 1615, 1857, 1891, 2072, 2506
TaiI ACGT 8 cut(s) 716, 809, 1361, 1368, 1602, 2712, 2856, 3063
TatI WGTACW 2 cut(s) 311, 2075
TfiI GAWTC 5 cut(s) 1091, 1517, 1772, 2862, 3149
TscAI CASTG 4 cut(s) 1693, 1860, 1974, 2240
TseFI GTSAC 4 cut(s) 2184, 2777, 2849, 2999
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 4 cut(s) 2184, 2777, 2849, 2999
TspGWI ACGGA 2 cut(s) 833, 1370
TspMI CCCGGG 1 cut(s) 2534
TspRI CASTG 4 cut(s) 1693, 1860, 1974, 2240
Tth111I GACNNNGTC 1 cut(s) 1270
Van91I CCANNNNNTGG 3 cut(s) 56, 254, 836
Vha464I CTTAAG 1 cut(s) 2176
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 4 cut(s) 47, 257, 2101, 3078
VspI ATTAAT 1 cut(s) 1322
XapI RAATTY 6 cut(s) 191, 2036, 2046, 2487, 3094, 3180
XceI RCATGY 5 cut(s) 368, 1438, 2185, 2318, 3000
XcmI CCANNNNNNNNNTGG 1 cut(s) 2452
XmaI CCCGGG 1 cut(s) 2534
XmiI GTMKAC 3 cut(s) 639, 803, 906
XmnI GAANNNNTTC 2 cut(s) 1489, 2333
XspI CTAG 1 cut(s) 2847
ZrmI AGTACT 2 cut(s) 313, 2077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.