Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004930

Physical Location & Seq

Genomic Coordinates Forward (+)

52859028 .. 52860695

Gene Structure 1668 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G028790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGACCATGAACATGATGACAATGTGCAAACAAACACAGACGTTACCAAACGTCCCTCTGTCCATTGGCTGCGGGCACAGCCACAACCTCCTCGATGTGTTGAATGTCCACCGCCAGAAGGATAAGGTGGTGATCGTAATGGGTGCCACCGGGACGGGCAAGTCAAAGCTCTCCATCGACCTGGCCACCCGCTTCCCGGCCGAAATCATCAACTCCGACAAAATGCAACTCTACAAGGGCCTTGACATCGCCACCAACAAAATATCACCTCAAGAACAACGCGGCGTCCCCCACCATTTGCTTGGAGTTTTAGACCCCAATGCGGATTTCTCTGCCGATGACTTCTGTGGCGTCACCTGCCGGACCGTCGAGTCGATCGTAAACCGCGACCGGCTTCCAATCATCGTTGGAGGCTCGAACTCGTACATAGAGGCGTTGATCGACGGCTACGATAAGATGGCGTTGATCGACGGCTACGATAAGATGTTCCGGTCAAGATATGACTGCTGCTTTCTCTGGGTGGATGTATCCTTACCCGTTTTGCAATCTTTTGTTTCCGACCGGGTCGATCAGATGGTGGAGAACGGAATGGTGGAGGAGGTGAGAGAGTTCTTTGATCCGAATGCGGATTATACAAAAGGGATCAGAAGGGCTATCGGTGTGCCGGAGTTTAATGACTTTTTCCGGTATGGTCCGTCTTTGGATGAGAAAACTAGAGCCTGGTTACTTGAACAAGCGATAAATGAAGTAAAAGACAATACATGCAAGTTGGCAGGTAGGCAATTGGAGAAGATTCGAAGACTTAGGAATAAAGGGTGGAGTCTGCATCCACTTGATGCCACTGAGGTGTTCCGGAAGCGTGGCAAGGAGGCTGATGAGGCCTGGGACAGGCTGGTTTCGGGGCCAAGCGCAGTGATTGTTCGCCAATTTCTTTACAATGTCAAGGCCGAAGTTCCCACAAATCTCACTGCCATCAGGGTTCCGGCCGCAATGGAAACTGCAGCTCTTGCGGCTGCAACTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009308 GO:0009451 GO:0009536 GO:0009690 GO:0009691 GO:0009824 GO:0009987 GO:0010467 GO:0010817 GO:0016070 GO:0016740 GO:0016765 GO:0034470 GO:0034641 GO:0034660 GO:0034754 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0052381 GO:0052622 GO:0052623 GO:0065007 GO:0065008 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 ko01100 ko01110 map00908 map01100 map01110

Pfam Domains

Protein Families

IPPT

Protein Analysis

341

Amino Acids

38.27

Weight (kDa)

6.34

Isoelectric Point (pI)

32.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IPT	PF01745	42 - 147	2.1e-06	Isopentenyl transferase
IPPT	PF01715	76 - 153	8.3e-24	IPP transferase
IPPT	PF01715	165 - 266	6.9e-14	IPP transferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004930)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63110
fragaria_vesca	FvH4_2g23840
malus_domestica	MD00G1084500.v1.1 MD03G1267900.v1.1 MD11G1288800.v1.1
prunus_persica	Prupe.5G047700_v2.0.a1 Prupe.8G243600_v2.0.a1
pyrus_communis	pycom06g03440
rosa_chinensis	RchiOBHm_Chr6g0291431 RchiOBHm_Chr6g0308751
rosa_laevigata	RLG00000010623 RLG00000010625 RLG00000012115
rosa_multiflora	Rmu_sc0003735.1_g000032
rosa_roxburghii	Rroxscaffold_7G00159980 Rroxscaffold_7G00176060
rosa_rugosa	Rorug06G0219300 Rorug06G0368000
rosa_samantha	Rh6AG331600 Rh6AG479000 Rh6AG479100 Rh6BG338500 Rh6BG488600 Rh6BG488700 Rh6CG344700 Rh6CG493900 Rh6DG331600 Rh6DG479500
rosa_wichuraiana	Rw6G028790 Rw6G041750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	365
AasI	GACNNNNNNGTC	2 cut(s)	160, 370
Acc36I	ACCTGC	2 cut(s)	365, 764
AccB1I	GGYRCC	1 cut(s)	143
AccII	CGCG	2 cut(s)	282, 387
AccIII	TCCGGA	1 cut(s)	852
AciI	CCGC	9 cut(s)	72, 112, 190, 282, 323, 385, 626, 987, 1010
AclWI	GGATC	2 cut(s)	611, 650
AcoI	YGGCCR	3 cut(s)	183, 198, 984
AcyI	GRCGYC	2 cut(s)	285, 351
AfaI	GTAC	1 cut(s)	425
AfiI	CCNNNNNNNGG	4 cut(s)	118, 196, 322, 888
AgsI	TTSAA	2 cut(s)	103, 731
AjnI	CCWGG	3 cut(s)	180, 719, 881
AjuI	GAANNNNNNNTTGG	2 cut(s)	311, 343
AleI	CACNNNNGTG	1 cut(s)	845
AluBI	AGCT	2 cut(s)	169, 1004
AluI	AGCT	2 cut(s)	169, 1004
AlwI	GGATC	2 cut(s)	611, 650
Aor13HI	TCCGGA	1 cut(s)	852
AoxI	GGCC	7 cut(s)	183, 198, 238, 879, 902, 945, 984
ApeKI	GCWGC	4 cut(s)	69, 507, 1001, 1013
AspLEI	GCGC	1 cut(s)	911
AspS9I	GGNCC	4 cut(s)	238, 363, 692, 902
AsuC2I	CCSGG	3 cut(s)	151, 197, 563
AsuHPI	GGTGA	4 cut(s)	142, 258, 346, 613
AsuII	TTCGAA	1 cut(s)	796
AvaII	GGWCC	2 cut(s)	363, 692
BaeGI	GKGCMC	1 cut(s)	78
BalI	TGGCCA	1 cut(s)	185
BanI	GGYRCC	1 cut(s)	143
BbsI	GAAGAC	1 cut(s)	805
BbvI	GCAGC	4 cut(s)	56, 494, 1000, 1013
BccI	CCATC	4 cut(s)	182, 451, 568, 980
BceAI	ACGGC	2 cut(s)	460, 487
BciT130I	CCWGG	3 cut(s)	182, 721, 883
BciVI	GTATCC	1 cut(s)	538
BcnI	CCSGG	3 cut(s)	151, 197, 563
BfaI	CTAG	2 cut(s)	714, 1024
BfmI	CTRYAG	1 cut(s)	999
BfuAI	ACCTGC	2 cut(s)	365, 764
BfuI	GTATCC	1 cut(s)	538
BisI	GCNGC	7 cut(s)	70, 283, 508, 987, 1002, 1011, 1014
BlsI	GCNGC	7 cut(s)	71, 284, 509, 988, 1003, 1012, 1015
Bme1390I	CCNGG	6 cut(s)	151, 182, 197, 563, 721, 883
Bme18I	GGWCC	2 cut(s)	363, 692
BmgT120I	GGNCC	4 cut(s)	238, 363, 692, 902
BmiI	GGNNCC	3 cut(s)	145, 903, 981
BmrFI	CCNGG	6 cut(s)	151, 182, 197, 563, 721, 883
BmsI	GCATC	2 cut(s)	826, 835
BpiI	GAAGAC	1 cut(s)	805
Bpu14I	TTCGAA	1 cut(s)	796
BpuEI	CTTGAG	1 cut(s)	255
BpuMI	CCSGG	3 cut(s)	151, 197, 563
BsaHI	GRCGYC	2 cut(s)	285, 351
BsaJI	CCNNGG	1 cut(s)	882
BsaWI	WCCGGW	3 cut(s)	489, 684, 852
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 196, 322, 888
Bse118I	RCCGGY	1 cut(s)	390
Bse3DI	GCAATG	1 cut(s)	996
BseAI	TCCGGA	1 cut(s)	852
BseBI	CCWGG	3 cut(s)	182, 721, 883
BseDI	CCNNGG	1 cut(s)	882
BseGI	GGATG	3 cut(s)	529, 709, 826
BseLI	CCNNNNNNNGG	4 cut(s)	118, 196, 322, 888
BseMI	GCAATG	1 cut(s)	996
BseMII	CTCAG	1 cut(s)	834
BseRI	GAGGAG	2 cut(s)	80, 611
BseSI	GKGCMC	1 cut(s)	78
BseX3I	CGGCCG	2 cut(s)	198, 984
BseXI	GCAGC	4 cut(s)	56, 494, 1000, 1013
Bsh1236I	CGCG	2 cut(s)	282, 387
Bsh1285I	CGRYCG	5 cut(s)	201, 378, 391, 562, 987
BshFI	GGCC	7 cut(s)	185, 200, 240, 881, 904, 947, 986
BshNI	GGYRCC	1 cut(s)	143
BsiEI	CGRYCG	5 cut(s)	201, 378, 391, 562, 987
BslFI	GGGAC	4 cut(s)	38, 166, 272, 899
BslI	CCNNNNNNNGG	4 cut(s)	118, 196, 322, 888
BsmFI	GGGAC	4 cut(s)	38, 166, 272, 899
BsmI	GAATGC	1 cut(s)	628
BsnI	GGCC	7 cut(s)	185, 200, 240, 881, 904, 947, 986
Bsp119I	TTCGAA	1 cut(s)	796
Bsp1286I	GDGCHC	1 cut(s)	78
Bsp13I	TCCGGA	1 cut(s)	852
Bsp143I	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
BspACI	CCGC	9 cut(s)	72, 112, 190, 282, 323, 385, 626, 987, 1010
BspANI	GGCC	7 cut(s)	185, 200, 240, 881, 904, 947, 986
BspCNI	CTCAG	1 cut(s)	835
BspEI	TCCGGA	1 cut(s)	852
BspFNI	CGCG	2 cut(s)	282, 387
BspLI	GGNNCC	3 cut(s)	145, 903, 981
BspMAI	CTGCAG	1 cut(s)	1003
BspMI	ACCTGC	2 cut(s)	365, 764
BspPI	GGATC	2 cut(s)	611, 650
BspT104I	TTCGAA	1 cut(s)	796
BspT107I	GGYRCC	1 cut(s)	143
BsrDI	GCAATG	1 cut(s)	996
BsrFI	RCCGGY	1 cut(s)	390
BssAI	RCCGGY	1 cut(s)	390
BssECI	CCNNGG	1 cut(s)	882
BssMI	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
BssNI	GRCGYC	2 cut(s)	285, 351
Bst2UI	CCWGG	3 cut(s)	182, 721, 883
Bst4CI	ACNGT	1 cut(s)	367
BstACI	GRCGYC	2 cut(s)	285, 351
BstAPI	GCANNNNNTGC	1 cut(s)	1007
BstBI	TTCGAA	1 cut(s)	796
BstC8I	GCNNGC	1 cut(s)	74
BstDEI	CTNAG	2 cut(s)	803, 843
BstENI	CCTNNNNNAGG	1 cut(s)	886
BstF5I	GGATG	3 cut(s)	529, 709, 826
BstFNI	CGCG	2 cut(s)	282, 387
BstHHI	GCGC	1 cut(s)	911
BstKTI	GATC	7 cut(s)	135, 378, 441, 468, 571, 619, 645
BstMBI	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
BstMCI	CGRYCG	5 cut(s)	201, 378, 391, 562, 987
BstMWI	GCNNNNNNNGC	5 cut(s)	78, 357, 878, 1007, 1010
BstNI	CCWGG	3 cut(s)	182, 721, 883
BstNSI	RCATGY	1 cut(s)	765
BstSCI	CCNGG	6 cut(s)	149, 180, 195, 561, 719, 881
BstSFI	CTRYAG	1 cut(s)	999
BstSLI	GKGCMC	1 cut(s)	78
BstUI	CGCG	2 cut(s)	282, 387
BstV1I	GCAGC	4 cut(s)	56, 494, 1000, 1013
BstV2I	GAAGAC	1 cut(s)	805
BstXI	CCANNNNNNTGG	2 cut(s)	181, 302
BstZI	CGGCCG	2 cut(s)	198, 984
BsuI	GTATCC	1 cut(s)	538
BsuRI	GGCC	7 cut(s)	185, 200, 240, 881, 904, 947, 986
BtgZI	GCGATG	1 cut(s)	232
BtsCI	GGATG	3 cut(s)	529, 709, 826
BtsI	GCAGTG	2 cut(s)	918, 966
BtsIMutI	CAGTG	3 cut(s)	840, 918, 966
BveI	ACCTGC	2 cut(s)	365, 764
Cac8I	GCNNGC	1 cut(s)	74
CfoI	GCGC	1 cut(s)	911
Cfr10I	RCCGGY	1 cut(s)	390
Cfr13I	GGNCC	4 cut(s)	238, 363, 692, 902
CpoI	CGGWCCG	1 cut(s)	363
CseI	GACGC	2 cut(s)	274, 340
Csp6I	GTAC	1 cut(s)	424
CspI	CGGWCCG	1 cut(s)	363
CviAII	CATG	3 cut(s)	7, 13, 762
CviQI	GTAC	1 cut(s)	424
DdeI	CTNAG	2 cut(s)	803, 843
DpnI	GATC	7 cut(s)	134, 377, 440, 467, 570, 618, 644
DpnII	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
DrdI	GACNNNNNNGTC	2 cut(s)	160, 370
DseDI	GACNNNNNNGTC	2 cut(s)	160, 370
EaeI	YGGCCR	3 cut(s)	183, 198, 984
EagI	CGGCCG	2 cut(s)	198, 984
EclXI	CGGCCG	2 cut(s)	198, 984
Eco147I	AGGCCT	1 cut(s)	881
Eco47I	GGWCC	2 cut(s)	363, 692
Eco52I	CGGCCG	2 cut(s)	198, 984
EcoNI	CCTNNNNNAGG	1 cut(s)	886
EcoO109I	RGGNCCY	1 cut(s)	238
EcoRII	CCWGG	3 cut(s)	180, 719, 881
FaeI	CATG	3 cut(s)	10, 16, 765
FaiI	YATR	7 cut(s)	8, 14, 428, 501, 633, 690, 763
FaqI	GGGAC	4 cut(s)	38, 166, 272, 899
FatI	CATG	3 cut(s)	6, 12, 761
FauI	CCCGC	2 cut(s)	65, 197
Fnu4HI	GCNGC	7 cut(s)	70, 283, 508, 987, 1002, 1011, 1014
FokI	GGATG	3 cut(s)	536, 716, 813
Fsp4HI	GCNGC	7 cut(s)	70, 283, 508, 987, 1002, 1011, 1014
FspBI	CTAG	2 cut(s)	714, 1024
GlaI	GCGC	1 cut(s)	910
GluI	GCNGC	7 cut(s)	70, 283, 508, 987, 1002, 1011, 1014
HaeIII	GGCC	7 cut(s)	185, 200, 240, 881, 904, 947, 986
HgaI	GACGC	2 cut(s)	274, 340
HhaI	GCGC	1 cut(s)	911
Hin1I	GRCGYC	2 cut(s)	285, 351
Hin1II	CATG	3 cut(s)	10, 16, 765
Hin6I	GCGC	1 cut(s)	909
HinP1I	GCGC	1 cut(s)	909
HinfI	GANTC	3 cut(s)	371, 793, 820
HphI	GGTGA	4 cut(s)	142, 258, 346, 613
Hpy166II	GTNNAC	2 cut(s)	109, 382
Hpy188I	TCNGA	5 cut(s)	217, 559, 573, 621, 647
Hpy188III	TCNNGA	3 cut(s)	272, 495, 853
Hpy8I	GTNNAC	2 cut(s)	109, 382
Hpy99I	CGWCG	3 cut(s)	371, 446, 473
HpyAV	CCTTC	2 cut(s)	112, 642
HpyCH4III	ACNGT	1 cut(s)	367
HpyCH4IV	ACGT	2 cut(s)	41, 51
HpyCH4V	TGCA	7 cut(s)	27, 226, 544, 765, 826, 1001, 1016
HpyF10VI	GCNNNNNNNGC	5 cut(s)	78, 357, 878, 1007, 1010
HpyF3I	CTNAG	2 cut(s)	803, 843
HpySE526I	ACGT	2 cut(s)	41, 51
Hsp92I	GRCGYC	2 cut(s)	285, 351
Hsp92II	CATG	3 cut(s)	10, 16, 765
HspAI	GCGC	1 cut(s)	909
Kpn2I	TCCGGA	1 cut(s)	852
Kzo9I	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
Lsp1109I	GCAGC	4 cut(s)	56, 494, 1000, 1013
LweI	GCATC	2 cut(s)	826, 835
MaeI	CTAG	2 cut(s)	714, 1024
MaeII	ACGT	2 cut(s)	41, 51
MaeIII	GTNAC	3 cut(s)	42, 352, 723
MalI	GATC	7 cut(s)	134, 377, 440, 467, 570, 618, 644
MboI	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
MboII	GAAGA	2 cut(s)	802, 810
MfeI	CAATTG	1 cut(s)	782
MhlI	GDGCHC	1 cut(s)	78
MlsI	TGGCCA	1 cut(s)	185
MluCI	AATT	2 cut(s)	782, 926
MluNI	TGGCCA	1 cut(s)	185
MlyI	GAGTC	2 cut(s)	380, 829
MmeI	TCCRAC	3 cut(s)	240, 388, 582
Mox20I	TGGCCA	1 cut(s)	185
MroI	TCCGGA	1 cut(s)	852
MscI	TGGCCA	1 cut(s)	185
MseI	TTAA	1 cut(s)	672
MslI	CAYNNNNRTG	2 cut(s)	11, 845
Msp20I	TGGCCA	1 cut(s)	185
MspR9I	CCNGG	6 cut(s)	151, 182, 197, 563, 721, 883
MunI	CAATTG	1 cut(s)	782
Mva1269I	GAATGC	1 cut(s)	628
MvaI	CCWGG	3 cut(s)	182, 721, 883
MvnI	CGCG	2 cut(s)	282, 387
MwoI	GCNNNNNNNGC	5 cut(s)	78, 357, 878, 1007, 1010
NciI	CCSGG	3 cut(s)	151, 197, 563
NdeII	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
NlaIII	CATG	3 cut(s)	10, 16, 765
NlaIV	GGNNCC	3 cut(s)	145, 903, 981
NmuCI	GTSAC	1 cut(s)	352
NspI	RCATGY	1 cut(s)	765
NspV	TTCGAA	1 cut(s)	796
OliI	CACNNNNGTG	1 cut(s)	845
PaqCI	CACCTGC	1 cut(s)	365
PceI	AGGCCT	1 cut(s)	881
PcsI	WCGNNNNNNNCGW	4 cut(s)	375, 384, 447, 474
PctI	GAATGC	1 cut(s)	628
PfeI	GAWTC	1 cut(s)	793
PflFI	GACNNNGTC	1 cut(s)	563
PkrI	GCNGC	7 cut(s)	71, 284, 509, 988, 1003, 1012, 1015
Ple19I	CGATCG	1 cut(s)	378
PleI	GAGTC	2 cut(s)	379, 828
PpsI	GAGTC	2 cut(s)	379, 828
Psp6I	CCWGG	3 cut(s)	180, 719, 881
PspGI	CCWGG	3 cut(s)	180, 719, 881
PspN4I	GGNNCC	3 cut(s)	145, 903, 981
PspPI	GGNCC	4 cut(s)	238, 363, 692, 902
PstI	CTGCAG	1 cut(s)	1003
PsyI	GACNNNGTC	1 cut(s)	563
PvuI	CGATCG	1 cut(s)	378
RsaI	GTAC	1 cut(s)	425
RsaNI	GTAC	1 cut(s)	424
RseI	CAYNNNNRTG	2 cut(s)	11, 845
Rsr2I	CGGWCCG	1 cut(s)	363
RsrII	CGGWCCG	1 cut(s)	363
SaqAI	TTAA	1 cut(s)	672
SatI	GCNGC	7 cut(s)	70, 283, 508, 987, 1002, 1011, 1014
Sau3AI	GATC	7 cut(s)	132, 375, 438, 465, 568, 616, 642
Sau96I	GGNCC	4 cut(s)	238, 363, 692, 902
SchI	GAGTC	2 cut(s)	380, 829
ScrFI	CCNGG	6 cut(s)	151, 182, 197, 563, 721, 883
SduI	GDGCHC	1 cut(s)	78
SfaNI	GCATC	2 cut(s)	826, 835
SfcI	CTRYAG	1 cut(s)	999
SfuI	TTCGAA	1 cut(s)	796
SinI	GGWCC	2 cut(s)	363, 692
SmiMI	CAYNNNNRTG	2 cut(s)	11, 845
SmlI	CTYRAG	1 cut(s)	270
SmoI	CTYRAG	1 cut(s)	270
Sse9I	AATT	2 cut(s)	782, 926
SseBI	AGGCCT	1 cut(s)	881
SsiI	CCGC	9 cut(s)	72, 112, 190, 282, 323, 385, 626, 987, 1010
SspMI	CTAG	2 cut(s)	714, 1024
StuI	AGGCCT	1 cut(s)	881
StyD4I	CCNGG	6 cut(s)	149, 180, 195, 561, 719, 881
TaaI	ACNGT	1 cut(s)	367
TaiI	ACGT	2 cut(s)	44, 54
TaqI	TCGA	9 cut(s)	93, 177, 369, 374, 416, 441, 468, 567, 796
TasI	AATT	2 cut(s)	782, 926
TauI	GCSGC	3 cut(s)	285, 989, 1013
TfiI	GAWTC	1 cut(s)	793
Tru1I	TTAA	1 cut(s)	672
Tru9I	TTAA	1 cut(s)	672
TscAI	CASTG	3 cut(s)	847, 918, 973
TseFI	GTSAC	1 cut(s)	352
TseI	GCWGC	4 cut(s)	69, 507, 1001, 1013
Tsp45I	GTSAC	1 cut(s)	352
TspDTI	ATGAA	2 cut(s)	23, 759
TspGWI	ACGGA	2 cut(s)	600, 684
TspRI	CASTG	3 cut(s)	847, 918, 973
Tth111I	GACNNNGTC	1 cut(s)	563
VpaK11BI	GGWCC	2 cut(s)	363, 692
XagI	CCTNNNNNAGG	1 cut(s)	886
XceI	RCATGY	1 cut(s)	765
XcmI	CCANNNNNNNNNTGG	1 cut(s)	299
XspI	CTAG	2 cut(s)	714, 1024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.