Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001752

Physical Location & Seq

Genomic Coordinates Forward (+)

57182787 .. 57191204

Gene Structure 8418 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G032950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5505 bp

ATGGACCTCCCTCAGTACCTTCACCACCATCCAAGGTACCCCCCACAGCTCCGCTCACAATCCCCCTCCTCCTTCAAATCCCTACCCCCTCCTCCGCCGCCACCACATCCCCAAAGGACCACCTTCTCCCCCCACCCCATCGATTCCGATCATTCCAGAACCTTCTCCCCTCGCACCACTCACCGCCCGCCGCCTCTCGATTCCGACCGGCCACGTCATCGCCTCTACCTCGAGTCCGGTCCGGATTACCGGGACCGATCCAGGAACTATTCGCCGCGCGATTACGACCGCGAATTGCATAAACCCTATAGGCGCGATCCGGACGGCGGCGCTAGGTTTAGATCGGAGTACGAAGAACAACGGGATCGTCGAGTTGATGACGGTTATCGCCTGGAGTTCCAGGATTCGGGTTCGGGTTGCTACGACGAGAGCGCGTATGAGGAGCTGTTGAAGAGTGGGAGGAGATATGAGGCGTATGAGATTCAAAGGTGGGGTCTTGAGAGGGCCCAATCGGAGGAGGATTTGTACGATCCTTTCGAATTGGATGAGCACGATGAGGAGGATCGGATTGTTTCGGAGACGAGGGATTATTATGGTTCCGAATCAGGAAGAAGTAGTAGTACTAGTAGATGGAAGCAGATACAAAAGAAGAGTGCCTTGCTTAGGCTTCAAACGCCGAAAACCCGGGACAGGAATTACTCTGCTTATTATGATAGTTCCAGCTCCGGTGGATCTTTCAGAGGGAAAGAACAGTATGAGTATGAGTATTTAGGTCATGGGAGGAAGGATGCAGTAGAAGAAGAGAGGCGTGGTGGTGGAGGAGAAGAGAGAGGCCAACAACAGAGAGGTGGGAGTAGTCCTCTGGACCTTGATGTTTCGTTTAAGTCGAATTCGCTGGTAGCCAAGCCGGTTAGAACTAGGGATGAGAAGAGAGGTTCAGTTTCAGATATGGATTGTTCAGAGTCACCACCTGCTAAGCCTTGTGAGGATGTTGTAAATTTGGATAGTTTGGCACTTGTTTCAAATAAGACATCGAGTCCTGGCAAGGAGGCCATGAAGTTGAAAGGAGAAGTTACTACTTCTGGCATGAATTCACAGCCTTGTTCTGTTGTGACTGATGATGCGTTTGTAAAGAGTGAAGTGGAAACAGATGTTGGTTCCAGTCAGAAGTCTTCTCCCAACGTTTCTAAGAAGACAAAAGTAGTGAAGAAAATAGTGGCCAAGAAGACAGTGAAAAAGGTCACAAACCCCCCCATTTCACAGCCAGAAAATAAGGAAGTGGAAGGTTCTCTGCCGAATGTTCTATCAGATGAATATGGCACTGTTCTCAACTCTTGTAGTAAGAATATTGGGGACAACTCAAGTGCAAATTTGGGCTCTTTGAGTCCTGCAGAGATTAATACTGATCAGAAGCACCCTCTGAATGTAGATACTCCGCAATGCACTGATTCAATCAAGGTAGCTACTTTTCCTGAAACTGGCGGTGTTGTGGATGTCAGCAAATCGATCTGTCAGAGTGGAAACATTTTATTGGTTGATAAAGTTGTACAAAAAGAATCATCACAGGCCATGTTGGCTGTAGAAGGCAATTTGAGCTCTGATTTTTTAAATTCAGATAAGATGCATGAACTCACGAATGTAAAGGGTTCTGAACATGGGGCTGAAACCTTGGAGAAACTGTCACATCAGGAAATCATTGTTCCTGATGTTGGCACTGTGGATGCAGTTAGCGAACAACCTTGTAGATATCAGCTCCCCACATCACTTCAAAATGGTGGTGTTGTGGAAGAACTTCCTAAGGCTATATCTTCAGCAGACAATAATATGGCGGTTGGTTTATCGAGTTCAGGGGAGACCAAAGCTGTCCGCTCCAAGATTGGTAGACGTACAAGGTGGGATTCTGATGAAGTTTATGTTAAGAAAGATAAGAGCATTACAGTTTCCAATGGTGGGAATACTAATTCTTTTGGCAAGCAGTCATTACCTGATTGTGACTCTAGATCTTTTGAAATTTGTGCCACAGAAAGATCTCCAAATATACCAAAATCAGTTGGAGATACTCATAGTGTTACACCTATGATTCAAAAGAAGAGAAAAGTTAAAACGCAGTTAGACTTTTCAAGGATATCCGACACTTGTGTAGACCCTGTAAATGTTTCTCCCCGCAAAGATGCACCGGATACAACAGTAAGTTCCTTCCTGAAGGATCCTAGTCATGCAGAAGTTTCTGTTTCTGGTGTTCAAAAGTTAGATATGGGATCACAGCCTGGTAATGATTGGGTCAGCATTCTGAATGGGAAAACTAGAATTGATGCTAACTATGGTACAAATGAGACTTCACCTGAGTATTTAAAGAGGAGAAAAGTGTCTGGCGCTCATCTGGTATTGACTTCAGCTCAAACCAATGGAGGACCTGCAAATAAATCTACATCTTATATACAGGAATCTTCAACTCATAATGATGTTCCAACATATCAAGCGGACAAAGGTGCTTCTTCTAGCATAGGTTCTCAGTGTGCTACTTCTAATTTAATTCCATCTCCTGAGGAAATCAATGTCTATCTTGAGGACATTATGGCTGCAGTATCATCTGATACTGTAGCTGCAGCTAGGGACTCCTTTACTAATGATGATATGAAGATTAAGCACCAAGGTATTCCACATATGCTAATCCCTTGTCCTTTACAATCACAGAATGAGGATAAAGAGGATGGTACTGAAGTTATGGTTGTAAATAATCATCACCTTGACATTTTGGATATAGATGGTAGTCATGAAAAAGACTTTGATGTTTGTGCAACAAATGAGCACATTATGGTTCAAGGTGAGACTGCTCCATGTACAATTCATTCAGAGCTTCAGCCTGCTGATTTGGGTATTAATTCTTTCAGTACAAATATAGAATCAGATTACCTCTGTGTTAAGGATAAACTGCCTTTTGTGCCAAGTTGTCTGCTTTCAATTGCTAATGGTAATGAAGTAACTTCTACCAACTCTATTGATGAAGGCATGAAGTCTGTGTCTGATACGCTGTCAGATACAGGTACTCCAGAAACTTCAACTAGTATCACTGACGTGCATATGCTGATTTGTAATCCTTCGGTTATTAAAAGTTTTGACGAAAAAGTTTGTGCGGATGACCAGAAATTTGAGGTGAAGTCTGAGGTTGCATCCGCTGGGAATTTATTCTCAGAGACCAAAACTAATTTAACATTAGATAATGTAACTGAAGGTCACCAATCAGTCACAGAGAAGGCAGTACCTTTGAAATTGCAGGATTCCAAAAAGACATCTCATGGTCTGCATCTCATAAGTGCTGAATCTGCATTGAAGAACCAGCTGGGTCAAGCTAGTCATAGGATTGTTCCAGGTCGTCCATTTTCTGCCTTTACTACCTCACAGAAGGCTACCTCTTCAGCACATATATCAAAGCCTCGGACTTGGCATCGAAATGCTAATTCATCTGTATCTCCTCTTCCTGTGAGTACTCTTCCACCTCAAAGGCAGTTGCCACAAAGAAATGGAAAACTTGAAAGTAATTCTTATGTTCGTAAAGGTAACACTCTTGTTAGAAGACGTGCTACAATTGCTGCTGTACCACAAAGCTCTCAAGGTCTCAGTTCATCTGTTCATCAGTTGAACTTTTCAGGTATAGATGGATTGAAGAAGAATGCAGGATCCGACAGTAGGGTTGACATTAAAAACCCTCCAAGAACAGGAGGACTCAATGCTTCTTCAGATAGGCCCCCTGCCCCACTACCCAGTGGAGTCAAAATGTCTGCATCTGCCGGCATGCAGAAGGATCAATCAAGGACTCGCTGGCAACTTCTGATACTCAGGAATACCATAGTGGTCCAGTTATATGTAAAGCGAAAATTAAATCAGTTGGTTGCATCATCTTCAAATCCGTCAGACCTTTCTGTCCACAATGTTGATAAGAACCAACCCTCAGATGGCTACTACAAGAGGCGCAAACATCAGCTAATTAGGTCTTCTTTGGAAAGTAATGGCAAAGAAACTGTTCTCCTGCCCACTGACAATCTAAACTCAGGGGAGCAAAAGGCTCGTAAGGTTATCCCCAGTAGAACATTTAACAAGAAGCGATCACTTAAGGCTGTTGCGAGGATGAGTAAAAAGAATTCCTTGGTCTGGACACCAAGTGGTTCACAGTCATCAAATAATAATGGTAGTTCATATGACCATCAGAAGGTTCTGCCTCAGCTGTTTCCGTGGAAAAGAGCAAGATACTGGAGAACTGTCATGCAAAGTCAGGCTTCCAATTTCAATTATAGTTCCTCATCAACAATCAGTAAAAAGTTGCTGCTCTCAAGGATGAGGGATACTGTATACACTAGGTCAACCCATGGATTTTCACTTAGAAAGTACAAGGTATTAAGTGTTGGTGGATCTAGTTTAAAATGGTCAAAATCCATTGAAAGTCGCTCAAAGAAAGTTAATGAGGAAGCTACACGGGCTGTTGCTGAAGTAGAGAAGAAGAAAAGAGAACACAATGGTGCTGCCTGCACTAGTTCTGGTTCTAAAATCAGAAACTCACCGGGAAAACGAATATTCCGCATTGGCTCTGTTCGTTACAAAATGGATCCTTCAAGGCGGACACTTAAGAGGATTTCAGATGACGAATCCTCAAAGTCTGTTGTGCTGAATCCAGAAACGGATGCCAAGAGATCATATGTTCCAAGGAGATTGGTGATTGGGAATGACGAATATGTGCGGATTGGAAATGGTAATCAGCTTATTAGAGATCCAAAGAAACGAACTCGCATATTGGCAAGTGAAAGAGTTAGATGGAGTTTGCACACTGCCAGACAGCGGTTGGCAAAAAAACGGAAATACTGCCAGTTTTTCACACGATTTGGGAAGTGTAACAAGGATGATGGAAAATGTCCCTATATTCATGACTCCTCCAAAATTGCAGTCTGCACAAAGTATCTGAACGGTTTATGTTCCAACCCCAACTGCAGATTAACCCACAAGGTCATTCCAGAAAGAATGCCAGACTGTTCTTTCTTTCTGCAAGGTTTATGCACCAATAAAAATTGTTCTTACAGACATGTAAATGTAAATCCGAAGGCTTCTACTTGTGAAGGATTTCTTAAAGGTTATTGTGCGGACGGAAATGAGTGCCGGAAGAAGCACAGCTATGTCTGCCAGACTTTTGAAGAAACAGGAATGTGTCCTCAAGGGCCCAAATGCAAGCTTCACCACCCCAAAAAGCGGATGAAGGGAAAGATAAAGAAACGATCAAGAGAACATAGAAATGGTTGGGGACGTTACTTTGTTTCCAAGGATGTTGGTGTTTCTGAGCCTGTAACAGCGTCTGCAAAGCACTGTGCACAAAATGGTGATGAGGCTGGAGAAAGTAACAATCCACCTGAGCAAACCACATTTTATGACAGTGATCCCTCTGAATTAGACTTGGATGATCTTGATGAACTAATTAAACCGGTTCGTTTGTTGAATAAGATGAAGACAAACATTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016973 GO:0031123 GO:0031124 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098787 GO:0098789 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1834

Amino Acids

203.49

Weight (kDa)

9.07

Isoelectric Point (pI)

51.04

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001752)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21580 AT1G21580 AT1G21580 AT1G21580 AT1G21580 AT1G21580 AT1G21580 AT1G21580 AT1G21580
fragaria_vesca	FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950 FvH4_2g27950
malus_domestica	MD08G1160000.v1.1 MD08G1160100.v1.1 MD08G1160200.v1.1 MD15G1133400.v1.1
prunus_persica	Prupe.1G489000_v2.0.a1 Prupe.1G489000_v2.0.a1 Prupe.1G489000_v2.0.a1
pyrus_communis	pycom08g13490 pycom08g13510
rosa_chinensis	RchiOBHm_Chr6g0296771
rosa_laevigata	RLG00000011660 RLG00000013665
rosa_multiflora	Rmu_ssc0000192.1_g000011
rosa_rugosa	Rorug06G0265800 Rorug06G0265800 Rorug06G0265900 Rorug06G0266000
rosa_samantha	Rh6AG376900 Rh6AG377000 Rh6BG385300 Rh6CG390500 Rh6DG377600
rosa_wichuraiana	Rw6G032950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	979
AasI	GACNNNNNNGTC	1 cut(s)	3920
Acc36I	ACCTGC	2 cut(s)	979, 2422
Acc65I	GGTACC	1 cut(s)	36
AccB1I	GGYRCC	1 cut(s)	36
AccBSI	CCGCTC	2 cut(s)	54, 1869
AccI	GTMKAC	3 cut(s)	1882, 2142, 4347
AccII	CGCG	5 cut(s)	277, 279, 291, 315, 434
AccIII	TCCGGA	2 cut(s)	241, 319
AclI	AACGTT	1 cut(s)	1182
AcoI	YGGCCR	2 cut(s)	209, 1218
AcsI	RAATTY	9 cut(s)	889, 997, 1090, 1369, 1609, 2010, 3146, 3181, 4138
AcuI	CTGAAG	9 cut(s)	1794, 2222, 2376, 2739, 2843, 3249, 3399, 3720, 4503
AdeI	CACNNNGTG	1 cut(s)	4160
AflII	CTTAAG	2 cut(s)	4109, 4619
AflIII	ACRYGT	1 cut(s)	5071
AgeI	ACCGGT	1 cut(s)	5464
AhdI	GACNNNNNGTC	1 cut(s)	1035
AhlI	ACTAGT	3 cut(s)	623, 3062, 4526
AjiI	CACGTC	3 cut(s)	215, 3076, 3578
AjnI	CCWGG	6 cut(s)	260, 390, 399, 1039, 2266, 3367
AjuI	GAANNNNNNNTTGG	2 cut(s)	1137, 1169
AleI	CACNNNNGTG	2 cut(s)	3074, 4512
Alw21I	GWGCWC	4 cut(s)	552, 1598, 2811, 5356
Alw26I	GTCTC	6 cut(s)	572, 1847, 2327, 2822, 3188, 3620
Alw44I	GTGCAC	1 cut(s)	5352
AlwNI	CAGNNNCTG	1 cut(s)	5339
Ama87I	CYCGRG	2 cut(s)	230, 684
Aor13HI	TCCGGA	2 cut(s)	241, 319
AoxI	GGCC	8 cut(s)	209, 504, 832, 1050, 1218, 1566, 3743, 5204
ApaI	GGGCCC	2 cut(s)	508, 5208
ApaLI	GTGCAC	1 cut(s)	5352
ApeKI	GCWGC	6 cut(s)	2579, 2603, 2606, 3590, 4321, 4517
ApoI	RAATTY	9 cut(s)	889, 997, 1090, 1369, 1609, 2010, 3146, 3181, 4138
ArsI	GACNNNNNNTTYG	6 cut(s)	478, 510, 3131, 3163, 4920, 4952
AseI	ATTAAT	2 cut(s)	1398, 2880
AsiGI	ACCGGT	1 cut(s)	5464
Asp700I	GAANNNNTTC	4 cut(s)	1791, 3185, 4020, 5109
Asp718I	GGTACC	1 cut(s)	36
AspLEI	GCGC	6 cut(s)	279, 315, 332, 434, 2375, 3972
AsuC2I	CCSGG	4 cut(s)	251, 685, 686, 4557
AsuII	TTCGAA	1 cut(s)	537
AvaI	CYCGRG	2 cut(s)	230, 684
AvaII	GGWCC	7 cut(s)	4, 117, 239, 253, 865, 2411, 3853
AxyI	CCTNAGG	2 cut(s)	1797, 2544
BaeGI	GKGCMC	3 cut(s)	508, 5208, 5356
BaeI	ACNNNNGTAYC	4 cut(s)	2172, 2205, 2706, 2739
BalI	TGGCCA	1 cut(s)	1220
BamHI	GGATCC	3 cut(s)	2206, 3677, 4600
BanI	GGYRCC	1 cut(s)	36
BanII	GRGCYC	4 cut(s)	508, 1379, 1598, 5208
BarI	GAAGNNNNNNTAC	6 cut(s)	604, 636, 3459, 3491, 3565, 3597
BbsI	GAAGAC	6 cut(s)	1164, 1199, 1232, 3580, 3984, 5495
Bbv12I	GWGCWC	4 cut(s)	552, 1598, 2811, 5356
BbvCI	CCTCAGC	1 cut(s)	4218
BbvI	GCAGC	6 cut(s)	2566, 2590, 2618, 3577, 4308, 4504
BceAI	ACGGC	1 cut(s)	340
BcgI	CGANNNNNNTGC	2 cut(s)	4046, 4080
BciT130I	CCWGG	6 cut(s)	262, 392, 401, 1041, 2268, 3369
BciVI	GTATCC	2 cut(s)	2173, 4333
BclI	TGATCA	1 cut(s)	1405
BcnI	CCSGG	4 cut(s)	251, 685, 686, 4557
BcoDI	GTCTC	6 cut(s)	572, 1847, 2327, 2822, 3188, 3620
BcuI	ACTAGT	3 cut(s)	623, 3062, 4526
BfmI	CTRYAG	7 cut(s)	307, 1389, 1578, 2580, 2598, 2604, 4978
BfoI	RGCGCY	2 cut(s)	333, 2376
BfrI	CTTAAG	2 cut(s)	4109, 4619
BfuAI	ACCTGC	2 cut(s)	979, 2422
BfuI	GTATCC	2 cut(s)	2173, 4333
BglI	GCCNNNNNGGC	1 cut(s)	1574
BglII	AGATCT	2 cut(s)	2000, 2027
BlpI	GCTNAGC	1 cut(s)	975
BmcAI	AGTACT	2 cut(s)	622, 3487
Bme18I	GGWCC	7 cut(s)	4, 117, 239, 253, 865, 2411, 3853
BmeRI	GACNNNNNGTC	1 cut(s)	1035
BmeT110I	CYCGRG	2 cut(s)	230, 684
BmgBI	CACGTC	3 cut(s)	215, 3076, 3578
BmrI	ACTGGG	2 cut(s)	3756, 4074
BmuI	ACTGGG	2 cut(s)	3756, 4074
BpiI	GAAGAC	6 cut(s)	1164, 1199, 1232, 3580, 3984, 5495
BplI	GAGNNNNNCTC	2 cut(s)	844, 876
BpmI	CTGGAG	4 cut(s)	413, 3033, 4270, 5394
Bpu10I	CCTNAGC	3 cut(s)	662, 4218, 5394
Bpu1102I	GCTNAGC	1 cut(s)	975
Bpu14I	TTCGAA	1 cut(s)	537
BpuEI	CTTGAG	6 cut(s)	518, 1345, 2585, 3594, 4312, 5184
BpuMI	CCSGG	4 cut(s)	251, 685, 686, 4557
Bsa29I	ATCGAT	2 cut(s)	141, 1505
BsaBI	GATNNNNATC	1 cut(s)	147
BsaI	GGTCTC	3 cut(s)	1847, 3188, 3620
BsaWI	WCCGGW	6 cut(s)	236, 241, 319, 725, 2176, 5464
BsaXI	ACNNNNNCTCC	6 cut(s)	509, 539, 1664, 1694, 3031, 3061
Bse118I	RCCGGY	4 cut(s)	207, 907, 3788, 5464
Bse1I	ACTGG	7 cut(s)	1161, 1483, 3762, 3856, 4080, 4253, 4858
Bse21I	CCTNAGG	2 cut(s)	1797, 2544
Bse3DI	GCAATG	1 cut(s)	1445
Bse8I	GATNNNNATC	1 cut(s)	147
BseAI	TCCGGA	2 cut(s)	241, 319
BseBI	CCWGG	6 cut(s)	262, 392, 401, 1041, 2268, 3369
BseCI	ATCGAT	2 cut(s)	141, 1505
BseJI	GATNNNNATC	1 cut(s)	147
BseMI	GCAATG	1 cut(s)	1445
BseNI	ACTGG	7 cut(s)	1161, 1483, 3762, 3856, 4080, 4253, 4858
BseSI	GKGCMC	3 cut(s)	508, 5208, 5356
BseXI	GCAGC	6 cut(s)	2566, 2590, 2618, 3577, 4308, 4504
BseYI	CCCAGC	2 cut(s)	3176, 3340
BsgI	GTGCAG	2 cut(s)	4507, 4924
Bsh1236I	CGCG	5 cut(s)	277, 279, 291, 315, 434
Bsh1285I	CGRYCG	2 cut(s)	208, 289
BshFI	GGCC	8 cut(s)	211, 506, 834, 1052, 1220, 1568, 3745, 5206
BshNI	GGYRCC	1 cut(s)	36
BshTI	ACCGGT	1 cut(s)	5464
BshVI	ATCGAT	2 cut(s)	141, 1505
BsiEI	CGRYCG	2 cut(s)	208, 289
BsiHKAI	GWGCWC	4 cut(s)	552, 1598, 2811, 5356
BsiHKCI	CYCGRG	2 cut(s)	230, 684
BslFI	GGGAC	6 cut(s)	266, 701, 1367, 2627, 4890, 5301
BsmAI	GTCTC	6 cut(s)	572, 1847, 2327, 2822, 3188, 3620
BsmBI	CGTCTC	1 cut(s)	572
BsmFI	GGGAC	6 cut(s)	266, 701, 1367, 2627, 4890, 5301
BsmI	GAATGC	3 cut(s)	2286, 3676, 5016
BsnI	GGCC	8 cut(s)	211, 506, 834, 1052, 1220, 1568, 3745, 5206
Bso31I	GGTCTC	3 cut(s)	1847, 3188, 3620
BsoBI	CYCGRG	2 cut(s)	230, 684
Bsp119I	TTCGAA	1 cut(s)	537
Bsp120I	GGGCCC	2 cut(s)	504, 5204
Bsp1286I	GDGCHC	7 cut(s)	508, 552, 1379, 1598, 2811, 5208, 5356
Bsp13I	TCCGGA	2 cut(s)	241, 319
Bsp1407I	TGTACA	2 cut(s)	1546, 2840
Bsp1720I	GCTNAGC	1 cut(s)	975
Bsp19I	CCATGG	1 cut(s)	4363
BspANI	GGCC	8 cut(s)	211, 506, 834, 1052, 1220, 1568, 3745, 5206
BspDI	ATCGAT	2 cut(s)	141, 1505
BspEI	TCCGGA	2 cut(s)	241, 319
BspFNI	CGCG	5 cut(s)	277, 279, 291, 315, 434
BspHI	TCATGA	2 cut(s)	2773, 4915
BspMAI	CTGCAG	4 cut(s)	1393, 2584, 2608, 4982
BspMI	ACCTGC	2 cut(s)	979, 2422
BspT104I	TTCGAA	1 cut(s)	537
BspT107I	GGYRCC	1 cut(s)	36
BspTI	CTTAAG	2 cut(s)	4109, 4619
BspTNI	GGTCTC	3 cut(s)	1847, 3188, 3620
BsrBI	CCGCTC	2 cut(s)	54, 1869
BsrDI	GCAATG	1 cut(s)	1445
BsrFI	RCCGGY	4 cut(s)	207, 907, 3788, 5464
BsrGI	TGTACA	2 cut(s)	1546, 2840
BsrI	ACTGG	7 cut(s)	1161, 1483, 3762, 3856, 4080, 4253, 4858
BssAI	RCCGGY	4 cut(s)	207, 907, 3788, 5464
BssNAI	GTATAC	1 cut(s)	4348
BssT1I	CCWWGG	7 cut(s)	32, 1668, 2650, 4143, 4363, 4697, 5304
Bst1107I	GTATAC	1 cut(s)	4348
Bst2UI	CCWGG	6 cut(s)	262, 392, 401, 1041, 2268, 3369
Bst6I	CTCTTC	9 cut(s)	446, 644, 795, 819, 923, 2084, 3418, 3480, 3495
BstAFI	CTTAAG	2 cut(s)	4109, 4619
BstAUI	TGTACA	2 cut(s)	1546, 2840
BstBI	TTCGAA	1 cut(s)	537
BstC8I	GCNNGC	8 cut(s)	188, 1973, 2865, 3790, 3794, 3821, 4522, 5216
BstDSI	CCRYGG	2 cut(s)	4229, 4363
BstEII	GGTNACC	1 cut(s)	3233
BstENI	CCTNNNNNAGG	1 cut(s)	661
BstFNI	CGCG	5 cut(s)	277, 279, 291, 315, 434
BstH2I	RGCGCY	2 cut(s)	333, 2376
BstHHI	GCGC	6 cut(s)	279, 315, 332, 434, 2375, 3972
BstMAI	GTCTC	6 cut(s)	572, 1847, 2327, 2822, 3188, 3620
BstMCI	CGRYCG	2 cut(s)	208, 289
BstMWI	GCNNNNNNNGC	8 cut(s)	673, 1574, 1593, 2941, 3323, 3587, 4472, 5166
BstNI	CCWGG	6 cut(s)	262, 392, 401, 1041, 2268, 3369
BstNSI	RCATGY	2 cut(s)	3796, 5075
BstPI	GGTNACC	1 cut(s)	3233
BstSFI	CTRYAG	7 cut(s)	307, 1389, 1578, 2580, 2598, 2604, 4978
BstSLI	GKGCMC	3 cut(s)	508, 5208, 5356
BstUI	CGCG	5 cut(s)	277, 279, 291, 315, 434
BstV1I	GCAGC	6 cut(s)	2566, 2590, 2618, 3577, 4308, 4504
BstV2I	GAAGAC	6 cut(s)	1164, 1199, 1232, 3580, 3984, 5495
BstX2I	RGATCY	8 cut(s)	731, 2000, 2027, 2206, 3677, 4406, 4600, 4762
BstYI	RGATCY	8 cut(s)	731, 2000, 2027, 2206, 3677, 4406, 4600, 4762
BstZ17I	GTATAC	1 cut(s)	4348
Bsu15I	ATCGAT	2 cut(s)	141, 1505
Bsu36I	CCTNAGG	2 cut(s)	1797, 2544
BsuI	GTATCC	2 cut(s)	2173, 4333
BsuRI	GGCC	8 cut(s)	211, 506, 834, 1052, 1220, 1568, 3745, 5206
BsuTUI	ATCGAT	2 cut(s)	141, 1505
BtgI	CCRYGG	2 cut(s)	4229, 4363
BtgZI	GCGATG	1 cut(s)	203
BtrI	CACGTC	3 cut(s)	215, 3076, 3578
BtsI	GCAGTG	1 cut(s)	4818
BveI	ACCTGC	2 cut(s)	979, 2422
Cac8I	GCNNGC	8 cut(s)	188, 1973, 2865, 3790, 3794, 3821, 4522, 5216
CaiI	CAGNNNCTG	1 cut(s)	5339
CciI	TCATGA	2 cut(s)	2773, 4915
CfoI	GCGC	6 cut(s)	279, 315, 332, 434, 2375, 3972
Cfr10I	RCCGGY	4 cut(s)	207, 907, 3788, 5464
Cfr9I	CCCGGG	1 cut(s)	684
ClaI	ATCGAT	2 cut(s)	141, 1505
CpoI	CGGWCCG	1 cut(s)	239
CseI	GACGC	1 cut(s)	5325
CspAI	ACCGGT	1 cut(s)	5464
CspI	CGGWCCG	1 cut(s)	239
DraI	TTTAAA	3 cut(s)	1608, 2352, 4416
DraIII	CACNNNGTG	1 cut(s)	4160
DrdI	GACNNNNNNGTC	1 cut(s)	3920
DriI	GACNNNNNGTC	1 cut(s)	1035
DseDI	GACNNNNNNGTC	1 cut(s)	3920
EaeI	YGGCCR	2 cut(s)	209, 1218
Eam1104I	CTCTTC	9 cut(s)	446, 644, 795, 819, 923, 2084, 3418, 3480, 3495
Eam1105I	GACNNNNNGTC	1 cut(s)	1035
EarI	CTCTTC	9 cut(s)	446, 644, 795, 819, 923, 2084, 3418, 3480, 3495
EciI	GGCGGA	2 cut(s)	84, 4627
Ecl136II	GAGCTC	1 cut(s)	1596
Eco130I	CCWWGG	7 cut(s)	32, 1668, 2650, 4143, 4363, 4697, 5304
Eco24I	GRGCYC	4 cut(s)	508, 1379, 1598, 5208
Eco31I	GGTCTC	3 cut(s)	1847, 3188, 3620
Eco32I	GATATC	2 cut(s)	1748, 2127
Eco47I	GGWCC	7 cut(s)	4, 117, 239, 253, 865, 2411, 3853
Eco53kI	GAGCTC	1 cut(s)	1596
Eco57I	CTGAAG	9 cut(s)	1794, 2222, 2376, 2739, 2843, 3249, 3399, 3720, 4503
Eco81I	CCTNAGG	2 cut(s)	1797, 2544
Eco88I	CYCGRG	2 cut(s)	230, 684
Eco91I	GGTNACC	1 cut(s)	3233
EcoICRI	GAGCTC	1 cut(s)	1596
EcoNI	CCTNNNNNAGG	1 cut(s)	661
EcoO109I	RGGNCCY	4 cut(s)	504, 2411, 3744, 5204
EcoO65I	GGTNACC	1 cut(s)	3233
EcoRI	GAATTC	3 cut(s)	889, 1090, 4138
EcoRII	CCWGG	6 cut(s)	260, 390, 399, 1039, 2266, 3367
EcoRV	GATATC	2 cut(s)	1748, 2127
EcoT14I	CCWWGG	7 cut(s)	32, 1668, 2650, 4143, 4363, 4697, 5304
EcoT22I	ATGCAT	1 cut(s)	1626
EcoT38I	GRGCYC	4 cut(s)	508, 1379, 1598, 5208
ErhI	CCWWGG	7 cut(s)	32, 1668, 2650, 4143, 4363, 4697, 5304
Esp3I	CGTCTC	1 cut(s)	572
FaqI	GGGAC	6 cut(s)	266, 701, 1367, 2627, 4890, 5301
FauI	CCCGC	2 cut(s)	195, 2171
FauNDI	CATATG	4 cut(s)	2664, 3081, 4195, 4690
FbaI	TGATCA	1 cut(s)	1405
FblI	GTMKAC	3 cut(s)	1882, 2142, 4347
FriOI	GRGCYC	4 cut(s)	508, 1379, 1598, 5208
GlaI	GCGC	6 cut(s)	278, 314, 331, 433, 2374, 3971
GsaI	CCCAGC	2 cut(s)	3180, 3344
GsuI	CTGGAG	4 cut(s)	413, 3033, 4270, 5394
HaeII	RGCGCY	2 cut(s)	333, 2376
HaeIII	GGCC	8 cut(s)	211, 506, 834, 1052, 1220, 1568, 3745, 5206
HgaI	GACGC	1 cut(s)	5325
HhaI	GCGC	6 cut(s)	279, 315, 332, 434, 2375, 3972
Hin6I	GCGC	6 cut(s)	277, 313, 330, 432, 2373, 3970
HinP1I	GCGC	6 cut(s)	277, 313, 330, 432, 2373, 3970
HincII	GTYRAC	2 cut(s)	3694, 4359
HindII	GTYRAC	2 cut(s)	3694, 4359
HindIII	AAGCTT	1 cut(s)	5216
Hpy166II	GTNNAC	8 cut(s)	1883, 2143, 3694, 3925, 4166, 4348, 4359, 5354
Hpy8I	GTNNAC	8 cut(s)	1883, 2143, 3694, 3925, 4166, 4348, 4359, 5354
Hpy99I	CGWCG	2 cut(s)	372, 428
HpyCH4IV	ACGT	6 cut(s)	214, 1182, 1885, 3075, 3577, 5290
HpyF10VI	GCNNNNNNNGC	8 cut(s)	673, 1574, 1593, 2941, 3323, 3587, 4472, 5166
HpySE526I	ACGT	6 cut(s)	214, 1182, 1885, 3075, 3577, 5290
HspAI	GCGC	6 cut(s)	277, 313, 330, 432, 2373, 3970
Kpn2I	TCCGGA	2 cut(s)	241, 319
KpnI	GGTACC	1 cut(s)	40
KroI	GCCGGC	1 cut(s)	3788
KroNI	GCCGGC	1 cut(s)	3790
Ksp22I	TGATCA	1 cut(s)	1405
LmnI	GCTCC	7 cut(s)	54, 442, 728, 1758, 1874, 2839, 4054
Lsp1109I	GCAGC	6 cut(s)	2566, 2590, 2618, 3577, 4308, 4504
MaeII	ACGT	6 cut(s)	214, 1182, 1885, 3075, 3577, 5290
MbiI	CCGCTC	2 cut(s)	54, 1869
MfeI	CAATTG	2 cut(s)	2961, 3585
MflI	RGATCY	8 cut(s)	731, 2000, 2027, 2206, 3677, 4406, 4600, 4762
MhlI	GDGCHC	7 cut(s)	508, 552, 1379, 1598, 2811, 5208, 5356
MlsI	TGGCCA	1 cut(s)	1220
MluNI	TGGCCA	1 cut(s)	1220
MmeI	TCCRAC	6 cut(s)	228, 2032, 2154, 2492, 3705, 4992
Mox20I	TGGCCA	1 cut(s)	1220
Mph1103I	ATGCAT	1 cut(s)	1626
MroI	TCCGGA	2 cut(s)	241, 319
MroNI	GCCGGC	1 cut(s)	3788
MroXI	GAANNNNTTC	4 cut(s)	1791, 3185, 4020, 5109
MscI	TGGCCA	1 cut(s)	1220
MslI	CAYNNNNRTG	7 cut(s)	2460, 3074, 3450, 4512, 5076, 5160, 5277
Msp20I	TGGCCA	1 cut(s)	1220
MspA1I	CMGCKG	4 cut(s)	3176, 3340, 4222, 4831
MspCI	CTTAAG	2 cut(s)	4109, 4619
MunI	CAATTG	2 cut(s)	2961, 3585
Mva1269I	GAATGC	3 cut(s)	2286, 3676, 5016
MvaI	CCWGG	6 cut(s)	262, 392, 401, 1041, 2268, 3369
MvnI	CGCG	5 cut(s)	277, 279, 291, 315, 434
MwoI	GCNNNNNNNGC	8 cut(s)	673, 1574, 1593, 2941, 3323, 3587, 4472, 5166
NaeI	GCCGGC	1 cut(s)	3790
NciI	CCSGG	4 cut(s)	251, 685, 686, 4557
NcoI	CCATGG	1 cut(s)	4363
NdeI	CATATG	4 cut(s)	2664, 3081, 4195, 4690
NgoMIV	GCCGGC	1 cut(s)	3788
NmuCI	GTSAC	7 cut(s)	963, 1111, 1240, 1680, 1991, 3233, 3244
NsiI	ATGCAT	1 cut(s)	1626
NspI	RCATGY	2 cut(s)	3796, 5075
NspV	TTCGAA	1 cut(s)	537
OliI	CACNNNNGTG	2 cut(s)	3074, 4512
PaeI	GCATGC	1 cut(s)	3796
PaeR7I	CTCGAG	1 cut(s)	230
PagI	TCATGA	2 cut(s)	2773, 4915
PaqCI	CACCTGC	1 cut(s)	979
PciI	ACATGT	1 cut(s)	5071
PcsI	WCGNNNNNNNCGW	3 cut(s)	367, 534, 884
PctI	GAATGC	3 cut(s)	2286, 3676, 5016
PdiI	GCCGGC	1 cut(s)	3790
PdmI	GAANNNNTTC	4 cut(s)	1791, 3185, 4020, 5109
PfoI	TCCNGGA	2 cut(s)	260, 399
PinAI	ACCGGT	1 cut(s)	5464
PpuMI	RGGWCCY	1 cut(s)	2411
PscI	ACATGT	1 cut(s)	5071
PshBI	ATTAAT	2 cut(s)	1398, 2880
Psp124BI	GAGCTC	1 cut(s)	1598
Psp1406I	AACGTT	1 cut(s)	1182
Psp5II	RGGWCCY	1 cut(s)	2411
Psp6I	CCWGG	6 cut(s)	260, 390, 399, 1039, 2266, 3367
PspEI	GGTNACC	1 cut(s)	3233
PspFI	CCCAGC	2 cut(s)	3176, 3340
PspGI	CCWGG	6 cut(s)	260, 390, 399, 1039, 2266, 3367
PspOMI	GGGCCC	2 cut(s)	504, 5204
PspPPI	RGGWCCY	1 cut(s)	2411
PspXI	VCTCGAGB	1 cut(s)	230
PstI	CTGCAG	4 cut(s)	1393, 2584, 2608, 4982
PstNI	CAGNNNCTG	1 cut(s)	5339
PsuI	RGATCY	8 cut(s)	731, 2000, 2027, 2206, 3677, 4406, 4600, 4762
PvuII	CAGCTG	2 cut(s)	3340, 4222
RseI	CAYNNNNRTG	7 cut(s)	2460, 3074, 3450, 4512, 5076, 5160, 5277
Rsr2I	CGGWCCG	1 cut(s)	239
RsrII	CGGWCCG	1 cut(s)	239
SacI	GAGCTC	1 cut(s)	1598
ScaI	AGTACT	2 cut(s)	622, 3487
SduI	GDGCHC	7 cut(s)	508, 552, 1379, 1598, 2811, 5208, 5356
SfcI	CTRYAG	7 cut(s)	307, 1389, 1578, 2580, 2598, 2604, 4978
Sfr274I	CTCGAG	1 cut(s)	230
SfuI	TTCGAA	1 cut(s)	537
SinI	GGWCC	7 cut(s)	4, 117, 239, 253, 865, 2411, 3853
SlaI	CTCGAG	1 cut(s)	230
SmaI	CCCGGG	1 cut(s)	686
SmiMI	CAYNNNNRTG	7 cut(s)	2460, 3074, 3450, 4512, 5076, 5160, 5277
SmlI	CTYRAG	9 cut(s)	230, 497, 1360, 2564, 3609, 4109, 4327, 4619, 5199
SmoI	CTYRAG	9 cut(s)	230, 497, 1360, 2564, 3609, 4109, 4327, 4619, 5199
SpeI	ACTAGT	3 cut(s)	623, 3062, 4526
SphI	GCATGC	1 cut(s)	3796
SspI	AATATT	2 cut(s)	1348, 4569
SstI	GAGCTC	1 cut(s)	1598
StyI	CCWWGG	7 cut(s)	32, 1668, 2650, 4143, 4363, 4697, 5304
TaiI	ACGT	6 cut(s)	217, 1185, 1888, 3078, 3580, 5293
TaqII	GACCGA	1 cut(s)	270
TatI	WGTACW	6 cut(s)	620, 1546, 2840, 2891, 3485, 4383
TauI	GCSGC	4 cut(s)	100, 193, 277, 330
TseFI	GTSAC	7 cut(s)	963, 1111, 1240, 1680, 1991, 3233, 3244
TseI	GCWGC	6 cut(s)	2579, 2603, 2606, 3590, 4321, 4517
Tsp45I	GTSAC	7 cut(s)	963, 1111, 1240, 1680, 1991, 3233, 3244
TspGWI	ACGGA	5 cut(s)	3897, 4218, 4688, 4861, 5148
TspMI	CCCGGG	1 cut(s)	684
Vha464I	CTTAAG	2 cut(s)	4109, 4619
VneI	GTGCAC	1 cut(s)	5352
VpaK11BI	GGWCC	7 cut(s)	4, 117, 239, 253, 865, 2411, 3853
VspI	ATTAAT	2 cut(s)	1398, 2880
XagI	CCTNNNNNAGG	1 cut(s)	661
XapI	RAATTY	9 cut(s)	889, 997, 1090, 1369, 1609, 2010, 3146, 3181, 4138
XbaI	TCTAGA	1 cut(s)	1997
XceI	RCATGY	2 cut(s)	3796, 5075
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3950, 4831
XhoI	CTCGAG	1 cut(s)	230
XmaI	CCCGGG	1 cut(s)	684
XmiI	GTMKAC	3 cut(s)	1882, 2142, 4347
XmnI	GAANNNNTTC	4 cut(s)	1791, 3185, 4020, 5109
ZrmI	AGTACT	2 cut(s)	622, 3487
Zsp2I	ATGCAT	1 cut(s)	1626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.