AT1G19940

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001649
Physical Location & Seq
Reverse (-)
6918159 .. 6921979
3821 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G19940.1

Sequence Viewer

Length: 1548 bp
ATGGTGGCGAAACCAAGGTCAAGATGTTGTTGTTGTAGTGTGTTCATCGGCGTTATCATCTTAATCGCTATAATCATCGCTGTCATTTTCACAATCAGACATAGATCAAACCACTCCGATGATGATGGCTCCAATGTTAAAAACTACGCTAATGCTCTCAAGATCGCTATGCAATTCTTCGATATCCAGAAATCTGGTAAGCTTGAAAACAACGAGATATCATGGAGAGGAGATTCAGGTCTTAAAGATGGAAGCGAAGCAAGTATCGATCTTTCGAAAGGTTTGTACGATGCTGGAGATCACATGAAGTTTGGTTTCCCAATGGCTTTCACTGCTACAGTTCTCTCATGGTCCATTCTTGAATACGGTGATCAAATGGCTTCTCTGAACTTACTGGATCATGCTAAAGACTCTCTCAAATGGACTACTGATTTTCTCATCAATGCTCATCCTTCTCCAAATGTCCTCTATATTCAGGTGGGAGATCCAGTGACGGATCATAAATGTTGGGATAGACCAGAAACAATGACTAGAAAGAGAACTCTTACTAAGATTGATACAAAGACTCCAGGGACTGAGGTTGCTGCAGAGACAGCAGCAGCTATGGCTGCAGCATCTTTAGTCTTTAAAGAAAGTGATACCAAGTATTCAAGCACGCTTTTGAAACATGCGAAGCAGTTGTTCGATTTTGCGGATAATAACAGAGGATCTTACAGTGTTAATATACCTGAGGTTCAGTCTTATTATAACTCAACTGGTTATGGAGATGAGCTTCTTTGGGCAGCTTCATGGTTGTACCATGCAACAGAGGATCAAACATATCTTGACTTTGTGTCGGAAAATGGTGAAGAGTTTGGTAATTTTGGAAGTCCATCATGGTTTAGTTGGGACAACAAGCTTCCAGGAACACATATACTATTATCAAGATTAACCTTTTTCAAAAAAGGGTTATCAGGAAGCAAAGGACTTCAAGGTTTTAAAGAAACAGCAGAAGCTGTCATGTGTGGGCTTATACCAAGCTCACCAACTGCTACATCTAGTAGAACCGATGGTGGTCTAATATGGGTAAGTGAATGGAATGCCCTGCAACACCCTGTGTCCTCTGCATTTCTAGCTACACTCTACAGTGACTATATGCTCACCTCTGGTGTTAAGGAACTATCTTGCAGTGACCAATCCTTTAAACCTTCAGATCTCAGAAAATTCGCTAGATCTCAGGCTGATTACATGTTAGGGAAAAATCCGGAGAAAATGAGCTATTTGGTGGGATATGGTGAGAAATATCCAGAATTTGTGCATCATAGAGGAGCTTCAATACCAGCTGATGCAACCACAGGATGCAAAGATGGGTTTAAGTGGCTTAACTCGGATGAACCAAATCCGAATGTAGCTTATGGAGCACTTGTTGGTGGACCGTTTTTGAATGACACGTTTATAGACGCAAGGAACAATTCTATGCAAAATGAGCCAAGCACTTATAACAGTGCTCTTGTTGTTGGTCTTTTGTCGAGTTTGGTTACTACTTCTTCCTCGGTAGAATCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

515

Amino Acids

56.71

Weight (kDa)

5.51

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 49 - 502 3.9e-158 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 747, 1479
AccIII TCCGGA 1 cut(s) 1243
AciI CCGC 1 cut(s) 692
AclWI GGATC 5 cut(s) 405, 479, 504, 715, 819
AcsI RAATTY 2 cut(s) 1202, 1289
AcuI CTGAAG 1 cut(s) 1173
AdeI CACNNNGTG 1 cut(s) 1096
AfaI GTAC 2 cut(s) 287, 797
AflIII ACRYGT 2 cut(s) 1227, 1428
AgsI TTSAA 8 cut(s) 206, 362, 651, 664, 940, 971, 1314, 1423
AhdI GACNNNNNGTC 1 cut(s) 832
AjnI CCWGG 2 cut(s) 568, 901
Alw21I GWGCWC 2 cut(s) 1402, 1489
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 5 cut(s) 405, 479, 504, 715, 819
AlwNI CAGNNNCTG 2 cut(s) 575, 995
Aor13HI TCCGGA 1 cut(s) 1243
ApeKI GCWGC 6 cut(s) 584, 596, 599, 608, 611, 782
ApoI RAATTY 2 cut(s) 1202, 1289
ArsI GACNNNNNNTTYG 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 351, 1412
AsuHPI GGTGA 5 cut(s) 380, 857, 1014, 1132, 1286
AsuII TTCGAA 1 cut(s) 275
AvaII GGWCC 2 cut(s) 351, 1412
AxyI CCTNAGG 1 cut(s) 729
Bbv12I GWGCWC 2 cut(s) 1402, 1489
BbvI GCAGC 6 cut(s) 571, 595, 608, 611, 623, 794
BccI CCATC 5 cut(s) 119, 242, 880, 1043, 1340
BciT130I CCWGG 2 cut(s) 570, 903
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 4 cut(s) 531, 1038, 1112, 1209
BfmI CTRYAG 4 cut(s) 336, 585, 609, 1123
BglII AGATCT 2 cut(s) 1192, 1211
BisI GCNGC 6 cut(s) 585, 597, 600, 609, 612, 783
BlsI GCNGC 6 cut(s) 586, 598, 601, 610, 613, 784
Bme1390I CCNGG 2 cut(s) 570, 903
Bme18I GGWCC 2 cut(s) 351, 1412
BmeRI GACNNNNNGTC 1 cut(s) 832
BmgT120I GGNCC 2 cut(s) 351, 1412
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 2 cut(s) 570, 903
BmsI GCATC 5 cut(s) 280, 623, 1306, 1315, 1328
BpmI CTGGAG 2 cut(s) 315, 552
Bpu14I TTCGAA 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 143
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 14, 569, 1530
BsaWI WCCGGW 1 cut(s) 1243
BsaXI ACNNNNNCTCC 6 cut(s) 474, 504, 550, 580, 1389, 1419
Bse1I ACTGG 3 cut(s) 399, 488, 760
Bse21I CCTNAGG 1 cut(s) 729
BseAI TCCGGA 1 cut(s) 1243
BseBI CCWGG 2 cut(s) 570, 903
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 14, 569, 1530
BseGI GGATG 3 cut(s) 448, 1343, 1375
BseMII CTCAG 4 cut(s) 567, 720, 1210, 1229
BseNI ACTGG 3 cut(s) 399, 488, 760
BseRI GAGGAG 2 cut(s) 243, 1320
BseXI GCAGC 6 cut(s) 571, 595, 608, 611, 623, 794
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 1402, 1489
BsiSI CCGG 1 cut(s) 1244
BslFI GGGAC 2 cut(s) 586, 902
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 2 cut(s) 586, 902
BsmI GAATGC 1 cut(s) 1084
Bsp119I TTCGAA 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 1402, 1489
Bsp13I TCCGGA 1 cut(s) 1243
BspACI CCGC 1 cut(s) 692
BspCNI CTCAG 4 cut(s) 568, 721, 1209, 1228
BspDI ATCGAT 1 cut(s) 267
BspEI TCCGGA 1 cut(s) 1243
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 2 cut(s) 589, 613
BspPI GGATC 5 cut(s) 405, 479, 504, 715, 819
BspT104I TTCGAA 1 cut(s) 275
BsrI ACTGG 3 cut(s) 399, 488, 760
BssECI CCNNGG 3 cut(s) 14, 569, 1530
BssT1I CCWWGG 1 cut(s) 14
Bst2UI CCWGG 2 cut(s) 570, 903
Bst4CI ACNGT 6 cut(s) 340, 368, 716, 1127, 1416, 1484
Bst6I CTCTTC 1 cut(s) 843
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 656
BstDEI CTNAG 5 cut(s) 549, 576, 729, 1196, 1215
BstF5I GGATG 3 cut(s) 448, 1343, 1375
BstMAI GTCTC 1 cut(s) 584
BstMWI GCNNNNNNNGC 7 cut(s) 332, 593, 605, 608, 1112, 1397, 1465
BstNI CCWGG 2 cut(s) 570, 903
BstNSI RCATGY 2 cut(s) 671, 1231
BstSCI CCNGG 2 cut(s) 568, 901
BstSFI CTRYAG 4 cut(s) 336, 585, 609, 1123
BstV1I GCAGC 6 cut(s) 571, 595, 608, 611, 623, 794
BstX2I RGATCY 4 cut(s) 484, 707, 1192, 1211
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 4 cut(s) 484, 707, 1192, 1211
Bsu15I ATCGAT 1 cut(s) 267
Bsu36I CCTNAGG 1 cut(s) 729
BsuTUI ATCGAT 1 cut(s) 267
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 3 cut(s) 448, 1343, 1375
BtsI GCAGTG 2 cut(s) 330, 1174
BtsIMutI CAGTG 6 cut(s) 330, 495, 721, 1132, 1174, 1489
Cac8I GCNNGC 1 cut(s) 656
CaiI CAGNNNCTG 2 cut(s) 575, 995
Cfr13I GGNCC 2 cut(s) 351, 1412
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 1 cut(s) 1448
Csp6I GTAC 2 cut(s) 286, 796
CviQI GTAC 2 cut(s) 286, 796
DdeI CTNAG 5 cut(s) 549, 576, 729, 1196, 1215
DraI TTTAAA 3 cut(s) 628, 979, 1183
DraIII CACNNNGTG 1 cut(s) 1096
DriI GACNNNNNGTC 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 843
Eam1105I GACNNNNNGTC 1 cut(s) 832
EarI CTCTTC 1 cut(s) 843
Eco130I CCWWGG 1 cut(s) 14
Eco32I GATATC 2 cut(s) 184, 219
Eco47I GGWCC 2 cut(s) 351, 1412
Eco57I CTGAAG 1 cut(s) 1173
Eco81I CCTNAGG 1 cut(s) 729
EcoRII CCWGG 2 cut(s) 568, 901
EcoRV GATATC 2 cut(s) 184, 219
EcoT14I CCWWGG 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 14
FaqI GGGAC 2 cut(s) 586, 902
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 6 cut(s) 585, 597, 600, 609, 612, 783
FokI GGATG 3 cut(s) 435, 1350, 1382
Fsp4HI GCNGC 6 cut(s) 585, 597, 600, 609, 612, 783
FspBI CTAG 4 cut(s) 531, 1038, 1112, 1209
GluI GCNGC 6 cut(s) 585, 597, 600, 609, 612, 783
GsuI CTGGAG 2 cut(s) 315, 552
HapII CCGG 1 cut(s) 1244
HgaI GACGC 1 cut(s) 1448
HindIII AAGCTT 2 cut(s) 200, 896
HinfI GANTC 4 cut(s) 233, 410, 565, 1538
HpaII CCGG 1 cut(s) 1244
HphI GGTGA 5 cut(s) 380, 857, 1014, 1132, 1286
Hpy166II GTNNAC 1 cut(s) 1412
Hpy188I TCNGA 8 cut(s) 98, 118, 387, 838, 1192, 1199, 1369, 1383
Hpy188III TCNNGA 9 cut(s) 21, 160, 187, 359, 824, 924, 954, 1244, 1286
Hpy8I GTNNAC 1 cut(s) 1412
HpyAV CCTTC 2 cut(s) 462, 1197
HpyCH4III ACNGT 6 cut(s) 340, 368, 716, 1127, 1416, 1484
HpyCH4IV ACGT 1 cut(s) 1430
HpyF10VI GCNNNNNNNGC 7 cut(s) 332, 593, 605, 608, 1112, 1397, 1465
HpyF3I CTNAG 5 cut(s) 549, 576, 729, 1196, 1215
HpySE526I ACGT 1 cut(s) 1430
Kpn2I TCCGGA 1 cut(s) 1243
Ksp22I TGATCA 1 cut(s) 370
LmnI GCTCC 3 cut(s) 134, 1307, 1397
Lsp1109I GCAGC 6 cut(s) 571, 595, 608, 611, 623, 794
LweI GCATC 5 cut(s) 280, 623, 1306, 1315, 1328
MaeI CTAG 4 cut(s) 531, 1038, 1112, 1209
MaeII ACGT 1 cut(s) 1430
MaeIII GTNAC 4 cut(s) 490, 1127, 1169, 1516
MboII GAAGA 3 cut(s) 169, 860, 1518
MflI RGATCY 4 cut(s) 484, 707, 1192, 1211
MhlI GDGCHC 2 cut(s) 1402, 1489
MluCI AATT 5 cut(s) 173, 859, 1202, 1289, 1450
MlyI GAGTC 2 cut(s) 404, 559
MmeI TCCRAC 1 cut(s) 816
MroI TCCGGA 1 cut(s) 1243
MslI CAYNNNNRTG 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 1322
MspI CCGG 1 cut(s) 1244
MspR9I CCNGG 2 cut(s) 570, 903
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 2 cut(s) 570, 903
MwoI GCNNNNNNNGC 7 cut(s) 332, 593, 605, 608, 1112, 1397, 1465
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 3 cut(s) 490, 1127, 1169
NspI RCATGY 2 cut(s) 671, 1231
NspV TTCGAA 1 cut(s) 275
PciI ACATGT 1 cut(s) 1227
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 2 cut(s) 233, 1538
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 6 cut(s) 586, 598, 601, 610, 613, 784
PleI GAGTC 2 cut(s) 404, 559
PpsI GAGTC 2 cut(s) 404, 559
PscI ACATGT 1 cut(s) 1227
PsiI TTATAA 2 cut(s) 747, 1479
Psp6I CCWGG 2 cut(s) 568, 901
PspGI CCWGG 2 cut(s) 568, 901
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 2 cut(s) 351, 1412
PstI CTGCAG 2 cut(s) 589, 613
PstNI CAGNNNCTG 2 cut(s) 575, 995
PsuI RGATCY 4 cut(s) 484, 707, 1192, 1211
PvuII CAGCTG 1 cut(s) 1322
RsaI GTAC 2 cut(s) 287, 797
RsaNI GTAC 2 cut(s) 286, 796
RseI CAYNNNNRTG 1 cut(s) 117
SatI GCNGC 6 cut(s) 585, 597, 600, 609, 612, 783
Sau96I GGNCC 2 cut(s) 351, 1412
SchI GAGTC 2 cut(s) 404, 559
ScrFI CCNGG 2 cut(s) 570, 903
SduI GDGCHC 2 cut(s) 1402, 1489
SfaNI GCATC 5 cut(s) 280, 623, 1306, 1315, 1328
SfcI CTRYAG 4 cut(s) 336, 585, 609, 1123
SfuI TTCGAA 1 cut(s) 275
SinI GGWCC 2 cut(s) 351, 1412
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 5 cut(s) 173, 859, 1202, 1289, 1450
SsiI CCGC 1 cut(s) 692
SspMI CTAG 4 cut(s) 531, 1038, 1112, 1209
StyD4I CCNGG 2 cut(s) 568, 901
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 6 cut(s) 340, 368, 716, 1127, 1416, 1484
TaiI ACGT 1 cut(s) 1433
TaqI TCGA 5 cut(s) 180, 267, 275, 684, 1508
TasI AATT 5 cut(s) 173, 859, 1202, 1289, 1450
TfiI GAWTC 2 cut(s) 233, 1538
TscAI CASTG 6 cut(s) 337, 495, 721, 1132, 1174, 1489
TseFI GTSAC 3 cut(s) 490, 1127, 1169
TseI GCWGC 6 cut(s) 584, 596, 599, 608, 611, 782
Tsp45I GTSAC 3 cut(s) 490, 1127, 1169
TspDTI ATGAA 4 cut(s) 34, 320, 777, 1386
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 6 cut(s) 337, 495, 721, 1132, 1174, 1489
VpaK11BI GGWCC 2 cut(s) 351, 1412
XapI RAATTY 2 cut(s) 1202, 1289
XceI RCATGY 2 cut(s) 671, 1231
XspI CTAG 4 cut(s) 531, 1038, 1112, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.