MD15G1013700.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001649
Physical Location & Seq
Forward (+)
746358 .. 749639
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1013700.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGGAGCTAAACCGAGGTGCAAGGGGTGGTGGGTGTGGGTGCTGGTGCTCGTGATTCTCGCTCTCGTCGCCGGCGGCGTCATTTTCGCGGTCGTCAAGAAAATAAAGAAAAACTCCGGCGATGACGACGGTGCGGTTCCTGGTCCTCCCGGCGCCATTACCCAGAAATACTCCGCCGCTCTCAAAGTCGCCGTCCAATTCTTCGACGTCCAGAAATCTGGTAAGCTGGTGGATAACAAGATATCGTGGAGAGGGGATTCGGGTCTGAAAGATGGAAGCGATGCGAAGTTGGATTTGTCCAAGGGAATGTATGACGCTGGGGATCACATGAAGTTCGGATTTCCAATGGCGTATACCGCCACGGTCTTGTCGTGGGCGATTCTTGAGTATGGTGATCACATGGATGCGGTGAATCAGTTAAAAACTTCTCAAGCCTCTCTTAAGTGGATCACTGACTTCCTTGTCAATGCTCATCCCAAGGACAATGTGCTCTACATTCAGGTGGGTGACCCTAAAAAGGACCATGAATGTTGGTATAGGCCGGAAGATGTGACTGAGAAGAGGCCGGCAACACAGGTCAACACATCCGCTCCAGGAACGGAGGTTGCGGCTGAAACTGCAGCAGCTATGGCTTCAGCATCTCTGGTCTTTAAGAAAAGTGATGCCAAATATTCAGATACGCTTCTTTCGCATGCCAAAAAGTTGTTTACTTTTGCTGACAAAAACAGAGGCTCTTACAGTGAAAGCATCCCCGAGGTCCAGAAATTTTACAATTCAACGGGATATGGAGATGAGCTTTTGTGGGCAGCAGCCTGGCTTTACCATGCGACTGGGGATAAGGAATACCTTGATTACGTCACAGGGGAGAATGGAGAGGAATTTGCTCAATTTGGTAAACCGACGTGGTTCAGTTGGGATAACAAGCTTGCAGGAACCCAGGTTTTGCTCTCCAGGTTGACCTTCTTTGGTGGGAAGGACACCTCAGACAATTCTGGCCTTCAAAAGTACAGGAAGACAGCTGAAGCTGTTATATGTGGCTTCCTGCCAAACTCGCCAACGGCCACGAGCAGCAGAACAGAGAATGGTCTGATCTGGGTCAGTGAATGGAATGCTCTGCAGCAGCCGGTAGCTGCTGCTTTCCTAGCTGCTCTTTACAGTGACTACATGCTTACATCTGGCTCCACAAAACTAGAATGCGATGGAGAAACCTATAAGCCATCGGATATTCGGAAATTTGTTAGATCTCAGGCGAATTATGTCTTGGGCGACAATCCTATGAAAATGAGCTATCTTGTCGGGTACGGGGATAAATATCCTGAATACGTACACCATAGAGGAGCTTCTATCCCCGCTAATAAGAAGACTAGCTGCAAAGAGGGATTCAAGTACCTTGACTCAACCAAACCCAACCCTAATGTAGCCGTTGGAGCACTTGTCGGCGGGCCGTTCCTAAACGAAACATACATTGATGCCCGGAACAACTCGAGGCAAGGGGAGCCGAGCACATACAACAGTGCCGTCGTTGTTGGCCTTCTGTCAAGTCTGGTGACCACCTCTTCGGCAGTTAAATCCTTCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

527

Amino Acids

57.55

Weight (kDa)

8.01

Isoelectric Point (pI)

24.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 57 - 512 2.5e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 2 cut(s) 179, 590
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 89
AclWI GGATC 2 cut(s) 330, 455
AcoI YGGCCR 1 cut(s) 1059
AcsI RAATTY 3 cut(s) 764, 878, 1232
AcuI CTGAAG 2 cut(s) 618, 1041
AcyI GRCGYC 3 cut(s) 78, 153, 207
AfaI GTAC 4 cut(s) 1007, 1301, 1326, 1388
AfiI CCNNNNNNNGG 1 cut(s) 517
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 3 cut(s) 777, 1001, 1384
AjiI CACGTC 1 cut(s) 903
AjnI CCWGG 5 cut(s) 139, 592, 812, 936, 950
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
Alw21I GWGCWC 4 cut(s) 51, 492, 1432, 1505
AlwI GGATC 2 cut(s) 330, 455
Ama87I CYCGRG 2 cut(s) 752, 1483
AoxI GGCC 6 cut(s) 539, 563, 994, 1059, 1442, 1528
ApoI RAATTY 3 cut(s) 764, 878, 1232
ArsI GACNNNNNNTTYG 4 cut(s) 177, 209, 1540, 1572
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 4 cut(s) 143, 520, 757, 1442
AsuC2I CCSGG 2 cut(s) 150, 1474
AsuHPI GGTGA 5 cut(s) 404, 421, 518, 1558, 1567
AvaI CYCGRG 2 cut(s) 752, 1483
AvaII GGWCC 3 cut(s) 143, 520, 757
BanI GGYRCC 1 cut(s) 152
BauI CACGAG 2 cut(s) 50, 1063
BbsI GAAGAC 2 cut(s) 1019, 1367
Bbv12I GWGCWC 4 cut(s) 51, 492, 1432, 1505
BccI CCATC 3 cut(s) 266, 1193, 1225
BceAI ACGGC 5 cut(s) 176, 1074, 1406, 1429, 1502
BciT130I CCWGG 5 cut(s) 141, 594, 814, 938, 952
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 2 cut(s) 150, 1474
BfaI CTAG 4 cut(s) 1142, 1190, 1365, 1579
BfmI CTRYAG 2 cut(s) 618, 1115
BfoI RGCGCY 1 cut(s) 156
BfrI CTTAAG 1 cut(s) 440
BglII AGATCT 1 cut(s) 1241
Bme1390I CCNGG 7 cut(s) 141, 150, 594, 814, 938, 952, 1474
Bme18I GGWCC 3 cut(s) 143, 520, 757
BmeT110I CYCGRG 2 cut(s) 752, 1483
BmgBI CACGTC 1 cut(s) 903
BmgT120I GGNCC 4 cut(s) 143, 520, 757, 1442
BmiI GGNNCC 5 cut(s) 138, 154, 934, 1180, 1497
BmrFI CCNGG 7 cut(s) 141, 150, 594, 814, 938, 952, 1474
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 6 cut(s) 271, 394, 647, 652, 756, 1459
BmuI ACTGGG 1 cut(s) 840
BpiI GAAGAC 2 cut(s) 1019, 1367
BpmI CTGGAG 2 cut(s) 576, 934
BpuEI CTTGAG 2 cut(s) 404, 414
BpuMI CCSGG 2 cut(s) 150, 1474
BsaAI YACGTR 1 cut(s) 1324
BsaBI GATNNNNATC 1 cut(s) 1311
BsaHI GRCGYC 3 cut(s) 78, 153, 207
BsaJI CCNNGG 6 cut(s) 14, 300, 360, 477, 753, 936
BsaXI ACNNNNNCTCC 4 cut(s) 574, 604, 1419, 1449
Bsc4I CCNNNNNNNGG 1 cut(s) 517
Bse118I RCCGGY 3 cut(s) 71, 565, 1123
Bse1I ACTGG 1 cut(s) 835
Bse8I GATNNNNATC 1 cut(s) 1311
BseBI CCWGG 5 cut(s) 141, 594, 814, 938, 952
BseDI CCNNGG 6 cut(s) 14, 300, 360, 477, 753, 936
BseGI GGATG 4 cut(s) 409, 472, 584, 747
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 1 cut(s) 517
BseMII CTCAG 3 cut(s) 546, 996, 1259
BseNI ACTGG 1 cut(s) 835
BseRI GAGGAG 1 cut(s) 1350
BseYI CCCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 6 cut(s) 541, 565, 996, 1061, 1444, 1530
BshNI GGYRCC 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 4 cut(s) 51, 492, 1432, 1505
BsiHKCI CYCGRG 2 cut(s) 752, 1483
BsiSI CCGG 7 cut(s) 72, 117, 150, 542, 566, 1124, 1474
BslI CCNNNNNNNGG 1 cut(s) 517
BsmI GAATGC 2 cut(s) 1114, 1199
BsnI GGCC 6 cut(s) 541, 565, 996, 1061, 1444, 1530
BsoBI CYCGRG 2 cut(s) 752, 1483
Bsp1286I GDGCHC 4 cut(s) 51, 492, 1432, 1505
Bsp143I GATC 5 cut(s) 322, 394, 447, 1089, 1241
BspANI GGCC 6 cut(s) 541, 565, 996, 1061, 1444, 1530
BspCNI CTCAG 3 cut(s) 547, 995, 1258
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 5 cut(s) 138, 154, 934, 1180, 1497
BspMAI CTGCAG 2 cut(s) 622, 1119
BspPI GGATC 2 cut(s) 330, 455
BspT107I GGYRCC 1 cut(s) 152
BspTI CTTAAG 1 cut(s) 440
BsrBI CCGCTC 2 cut(s) 179, 590
BsrFI RCCGGY 3 cut(s) 71, 565, 1123
BsrI ACTGG 1 cut(s) 835
BssAI RCCGGY 3 cut(s) 71, 565, 1123
BssECI CCNNGG 6 cut(s) 14, 300, 360, 477, 753, 936
BssMI GATC 5 cut(s) 322, 394, 447, 1089, 1241
BssNAI GTATAC 1 cut(s) 354
BssNI GRCGYC 3 cut(s) 78, 153, 207
BssSI CACGAG 2 cut(s) 50, 1063
BssT1I CCWWGG 2 cut(s) 300, 477
Bst1107I GTATAC 1 cut(s) 354
Bst2BI CACGAG 2 cut(s) 50, 1063
Bst2UI CCWGG 5 cut(s) 141, 594, 814, 938, 952
Bst4CI ACNGT 5 cut(s) 131, 364, 740, 1157, 1514
Bst6I CTCTTC 2 cut(s) 554, 1561
BstACI GRCGYC 3 cut(s) 78, 153, 207
BstAFI CTTAAG 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 1324
BstC8I GCNNGC 5 cut(s) 73, 567, 693, 927, 1442
BstDEI CTNAG 3 cut(s) 555, 982, 1245
BstDSI CCRYGG 1 cut(s) 360
BstEII GGTNACC 2 cut(s) 506, 1546
BstF5I GGATG 4 cut(s) 409, 472, 584, 747
BstFNI CGCG 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 5 cut(s) 325, 397, 450, 1092, 1244
BstMBI GATC 5 cut(s) 322, 394, 447, 1089, 1241
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 9 cut(s) 68, 356, 617, 629, 688, 1051, 1142, 1427, 1495
BstNI CCWGG 5 cut(s) 141, 594, 814, 938, 952
BstNSI RCATGY 2 cut(s) 695, 1168
BstPI GGTNACC 2 cut(s) 506, 1546
BstSCI CCNGG 7 cut(s) 139, 148, 592, 812, 936, 950, 1472
BstSFI CTRYAG 2 cut(s) 618, 1115
BstSNI TACGTA 1 cut(s) 1324
BstUI CGCG 1 cut(s) 89
BstV2I GAAGAC 2 cut(s) 1019, 1367
BstX2I RGATCY 1 cut(s) 1241
BstXI CCANNNNNNTGG 2 cut(s) 218, 830
BstYI RGATCY 1 cut(s) 1241
BstZ17I GTATAC 1 cut(s) 354
BsuRI GGCC 6 cut(s) 541, 565, 996, 1061, 1444, 1530
BtgI CCRYGG 1 cut(s) 360
BtgZI GCGATG 3 cut(s) 135, 294, 1212
BtrI CACGTC 1 cut(s) 903
BtsCI GGATG 4 cut(s) 409, 472, 584, 747
BtsIMutI CAGTG 5 cut(s) 450, 745, 1105, 1162, 1519
Cac8I GCNNGC 5 cut(s) 73, 567, 693, 927, 1442
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 3 cut(s) 71, 565, 1123
Cfr13I GGNCC 4 cut(s) 143, 520, 757, 1442
CseI GACGC 2 cut(s) 67, 323
Csp6I GTAC 4 cut(s) 1006, 1300, 1325, 1387
CviAII CATG 6 cut(s) 328, 400, 524, 692, 824, 1165
CviQI GTAC 4 cut(s) 1006, 1300, 1325, 1387
DdeI CTNAG 3 cut(s) 555, 982, 1245
DinI GGCGCC 1 cut(s) 154
DpnI GATC 5 cut(s) 324, 396, 449, 1091, 1243
DpnII GATC 5 cut(s) 322, 394, 447, 1089, 1241
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 1 cut(s) 1059
Eam1104I CTCTTC 2 cut(s) 554, 1561
EarI CTCTTC 2 cut(s) 554, 1561
EciI GGCGGA 1 cut(s) 163
Eco105I TACGTA 1 cut(s) 1324
Eco130I CCWWGG 2 cut(s) 300, 477
Eco32I GATATC 1 cut(s) 243
Eco47I GGWCC 3 cut(s) 143, 520, 757
Eco57I CTGAAG 2 cut(s) 618, 1041
Eco88I CYCGRG 2 cut(s) 752, 1483
Eco91I GGTNACC 2 cut(s) 506, 1546
EcoO65I GGTNACC 2 cut(s) 506, 1546
EcoRII CCWGG 5 cut(s) 139, 592, 812, 936, 950
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 2 cut(s) 300, 477
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 300, 477
FaeI CATG 6 cut(s) 331, 403, 527, 695, 827, 1168
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FatI CATG 6 cut(s) 327, 399, 523, 691, 823, 1164
FauI CCCGC 2 cut(s) 1357, 1433
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 353
FokI GGATG 4 cut(s) 416, 459, 571, 734
FspBI CTAG 4 cut(s) 1142, 1190, 1365, 1579
GlaI GCGC 1 cut(s) 154
GsaI CCCAGC 1 cut(s) 321
GsuI CTGGAG 2 cut(s) 576, 934
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 6 cut(s) 541, 565, 996, 1061, 1444, 1530
HapII CCGG 7 cut(s) 72, 117, 150, 542, 566, 1124, 1474
HgaI GACGC 2 cut(s) 67, 323
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 3 cut(s) 78, 153, 207
Hin1II CATG 6 cut(s) 331, 403, 527, 695, 827, 1168
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HincII GTYRAC 2 cut(s) 580, 957
HindII GTYRAC 2 cut(s) 580, 957
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 6 cut(s) 55, 257, 379, 412, 1380, 1394
HpaII CCGG 7 cut(s) 72, 117, 150, 542, 566, 1124, 1474
HphI GGTGA 5 cut(s) 404, 421, 518, 1558, 1567
Hpy166II GTNNAC 6 cut(s) 354, 580, 708, 896, 957, 1327
Hpy188I TCNGA 7 cut(s) 267, 338, 676, 985, 1089, 1222, 1230
Hpy188III TCNNGA 6 cut(s) 52, 97, 211, 383, 760, 1316
Hpy8I GTNNAC 6 cut(s) 354, 580, 708, 896, 957, 1327
Hpy99I CGWCG 5 cut(s) 71, 131, 209, 904, 1523
HpyAV CCTTC 4 cut(s) 967, 970, 1007, 1541
HpyCH4III ACNGT 5 cut(s) 131, 364, 740, 1157, 1514
HpyCH4IV ACGT 4 cut(s) 207, 855, 902, 1323
HpyCH4V TGCA 5 cut(s) 21, 620, 929, 1117, 1371
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 356, 617, 629, 688, 1051, 1142, 1427, 1495
HpyF3I CTNAG 3 cut(s) 555, 982, 1245
HpySE526I ACGT 4 cut(s) 207, 855, 902, 1323
Hsp92I GRCGYC 3 cut(s) 78, 153, 207
Hsp92II CATG 6 cut(s) 331, 403, 527, 695, 827, 1168
HspAI GCGC 1 cut(s) 153
KasI GGCGCC 1 cut(s) 152
KroI GCCGGC 2 cut(s) 71, 565
KroNI GCCGGC 2 cut(s) 73, 567
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 5 cut(s) 322, 394, 447, 1089, 1241
LmnI GCTCC 6 cut(s) 5, 595, 1184, 1337, 1427, 1495
LweI GCATC 6 cut(s) 271, 394, 647, 652, 756, 1459
MaeI CTAG 4 cut(s) 1142, 1190, 1365, 1579
MaeII ACGT 4 cut(s) 207, 855, 902, 1323
MaeIII GTNAC 5 cut(s) 506, 550, 856, 1157, 1546
MalI GATC 5 cut(s) 324, 396, 449, 1091, 1243
MbiI CCGCTC 2 cut(s) 179, 590
MboI GATC 5 cut(s) 322, 394, 447, 1089, 1241
MboII GAAGA 6 cut(s) 193, 557, 571, 1024, 1372, 1548
MflI RGATCY 1 cut(s) 1241
MhlI GDGCHC 4 cut(s) 51, 492, 1432, 1505
MluCI AATT 8 cut(s) 197, 764, 772, 878, 887, 988, 1232, 1252
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 1 cut(s) 1388
MmeI TCCRAC 2 cut(s) 270, 1405
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 2 cut(s) 71, 565
MseI TTAA 4 cut(s) 419, 441, 651, 1566
MslI CAYNNNNRTG 2 cut(s) 402, 500
MspA1I CMGCKG 1 cut(s) 1019
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 7 cut(s) 72, 117, 150, 542, 566, 1124, 1474
MspR9I CCNGG 7 cut(s) 141, 150, 594, 814, 938, 952, 1474
Mva1269I GAATGC 2 cut(s) 1114, 1199
MvaI CCWGG 5 cut(s) 141, 594, 814, 938, 952
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 9 cut(s) 68, 356, 617, 629, 688, 1051, 1142, 1427, 1495
NaeI GCCGGC 2 cut(s) 73, 567
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 2 cut(s) 150, 1474
NdeII GATC 5 cut(s) 322, 394, 447, 1089, 1241
NgoMIV GCCGGC 2 cut(s) 71, 565
NlaIII CATG 6 cut(s) 331, 403, 527, 695, 827, 1168
NlaIV GGNNCC 5 cut(s) 138, 154, 934, 1180, 1497
NmeAIII GCCGAG 1 cut(s) 1524
NmuCI GTSAC 5 cut(s) 506, 550, 856, 1157, 1546
NspI RCATGY 2 cut(s) 695, 1168
PaeI GCATGC 1 cut(s) 695
PaeR7I CTCGAG 1 cut(s) 1483
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 368
PctI GAATGC 2 cut(s) 1114, 1199
PdiI GCCGGC 2 cut(s) 73, 567
PfeI GAWTC 5 cut(s) 55, 257, 379, 412, 1380
PfoI TCCNGGA 1 cut(s) 592
PleI GAGTC 1 cut(s) 1388
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 1388
Ppu21I YACGTR 1 cut(s) 1324
Psp6I CCWGG 5 cut(s) 139, 592, 812, 936, 950
PspEI GGTNACC 2 cut(s) 506, 1546
PspFI CCCAGC 1 cut(s) 317
PspGI CCWGG 5 cut(s) 139, 592, 812, 936, 950
PspN4I GGNNCC 5 cut(s) 138, 154, 934, 1180, 1497
PspPI GGNCC 4 cut(s) 143, 520, 757, 1442
PspXI VCTCGAGB 1 cut(s) 1483
PstI CTGCAG 2 cut(s) 622, 1119
PsuI RGATCY 1 cut(s) 1241
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 4 cut(s) 1007, 1301, 1326, 1388
RsaNI GTAC 4 cut(s) 1006, 1300, 1325, 1387
RseI CAYNNNNRTG 2 cut(s) 402, 500
SaqAI TTAA 4 cut(s) 419, 441, 651, 1566
Sau3AI GATC 5 cut(s) 322, 394, 447, 1089, 1241
Sau96I GGNCC 4 cut(s) 143, 520, 757, 1442
SchI GAGTC 1 cut(s) 1388
ScrFI CCNGG 7 cut(s) 141, 150, 594, 814, 938, 952, 1474
SduI GDGCHC 4 cut(s) 51, 492, 1432, 1505
SfaNI GCATC 6 cut(s) 271, 394, 647, 652, 756, 1459
SfcI CTRYAG 2 cut(s) 618, 1115
SfoI GGCGCC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 1483
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 3 cut(s) 143, 520, 757
SlaI CTCGAG 1 cut(s) 1483
SmiMI CAYNNNNRTG 2 cut(s) 402, 500
SmlI CTYRAG 4 cut(s) 383, 429, 440, 1483
SmoI CTYRAG 4 cut(s) 383, 429, 440, 1483
SnaBI TACGTA 1 cut(s) 1324
SphI GCATGC 1 cut(s) 695
Sse9I AATT 8 cut(s) 197, 764, 772, 878, 887, 988, 1232, 1252
SspDI GGCGCC 1 cut(s) 152
SspI AATATT 1 cut(s) 671
SspMI CTAG 4 cut(s) 1142, 1190, 1365, 1579
StyD4I CCNGG 7 cut(s) 139, 148, 592, 812, 936, 950, 1472
StyI CCWWGG 2 cut(s) 300, 477
TaaI ACNGT 5 cut(s) 131, 364, 740, 1157, 1514
TaiI ACGT 4 cut(s) 210, 858, 905, 1326
TaqI TCGA 2 cut(s) 204, 1484
TasI AATT 8 cut(s) 197, 764, 772, 878, 887, 988, 1232, 1252
TatI WGTACW 1 cut(s) 1005
TauI GCSGC 3 cut(s) 78, 179, 611
TfiI GAWTC 5 cut(s) 55, 257, 379, 412, 1380
Tru1I TTAA 4 cut(s) 419, 441, 651, 1566
Tru9I TTAA 4 cut(s) 419, 441, 651, 1566
TscAI CASTG 5 cut(s) 457, 745, 1105, 1162, 1519
TseFI GTSAC 5 cut(s) 506, 550, 856, 1157, 1546
Tsp45I GTSAC 5 cut(s) 506, 550, 856, 1157, 1546
TspDTI ATGAA 3 cut(s) 344, 540, 1292
TspGWI ACGGA 1 cut(s) 614
TspRI CASTG 5 cut(s) 457, 745, 1105, 1162, 1519
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 3 cut(s) 143, 520, 757
XapI RAATTY 3 cut(s) 764, 878, 1232
XceI RCATGY 2 cut(s) 695, 1168
XhoI CTCGAG 1 cut(s) 1483
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 4 cut(s) 1142, 1190, 1365, 1579
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.