RchiOBHm_Chr2g0099551

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001649
Physical Location & Seq
Forward (+)
11735861 .. 11736828
968 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47427

Sequence Viewer

Length: 561 bp
ATGGTGGAAGGAGAAGAAAAATCTTATCACAGAGAGTCGAACACAAGCTGGGGTTGGCGGTTAATTCTGTTGCTATATATATCTCTCGTCGCCGCAGCTGCTACCCTCACTCTGCTAAGAAACTTCAACCGCTACTTCCATTCAAGTCAGGTGACTCAGCTCTCGGCTCGACCCGGACATGTAGTACAAAACTACGCCAACGCTCTTCAACTCGCCACCCAATTCTTCGACGCCCAAAAATCTGGGAAGCTGATGAAGAACCGGGTCGAGTGGAGAGGCGACTTGGGGCTCCAAGATGGGAGTGAGGAGGGGTTGGACTTATCAAAGGGGATGTATGATGCTGGTGATCTTTTAAAGTTTGTGTTTCCTATGGCTTTCATTGCAACAATTTTGTCTTGGGCTATTCTTGAATATGGGGACCAAATGAAAGAGGTGAAGCAATTGGGTCAAGCCCATGACTCGCTGAAGTGGATCACTGATTACTTGATCAATGCACATCCTTCCCCAAATGTGCTCTATATCCAGGTAGGTGATCCTGAATTAGACCACAATTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

186

Amino Acids

21.15

Weight (kDa)

5.63

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 65 - 186 2.4e-46 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 58, 93, 130
AclWI GGATC 2 cut(s) 479, 527
AcuI CTGAAG 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 186
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 4 cut(s) 127, 144, 209, 410
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 4 cut(s) 48, 98, 160, 250
AluI AGCT 4 cut(s) 48, 98, 160, 250
Alw21I GWGCWC 1 cut(s) 516
AlwI GGATC 2 cut(s) 479, 527
ApeKI GCWGC 2 cut(s) 95, 98
AspS9I GGNCC 1 cut(s) 418
AsuC2I CCSGG 2 cut(s) 174, 263
AsuHPI GGTGA 4 cut(s) 163, 356, 445, 542
AvaII GGWCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 85, 107
BccI CCATC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 524
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 2 cut(s) 174, 263
BisI GCNGC 3 cut(s) 93, 96, 99
BlsI GCNGC 3 cut(s) 94, 97, 100
Bme1390I CCNGG 3 cut(s) 174, 263, 524
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 2 cut(s) 290, 419
BmrFI CCNGG 3 cut(s) 174, 263, 524
BmsI GCATC 1 cut(s) 328
BpuMI CCSGG 2 cut(s) 174, 263
BsaHI GRCGYC 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 524
BseGI GGATG 2 cut(s) 336, 496
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 85, 107
BseYI CCCAGC 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 516
BsiSI CCGG 2 cut(s) 174, 262
BslFI GGGAC 1 cut(s) 431
BsmFI GGGAC 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 291, 516
Bsp143I GATC 4 cut(s) 346, 471, 486, 532
BspACI CCGC 3 cut(s) 58, 93, 130
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 290, 419
BspPI GGATC 2 cut(s) 479, 527
BspQI GCTCTTC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 378
BssMI GATC 4 cut(s) 346, 471, 486, 532
BssNI GRCGYC 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 524
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 116, 156
BstF5I GGATG 2 cut(s) 336, 496
BstKTI GATC 4 cut(s) 349, 474, 489, 535
BstMBI GATC 4 cut(s) 346, 471, 486, 532
BstMWI GCNNNNNNNGC 2 cut(s) 98, 380
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 3 cut(s) 172, 261, 522
BstV1I GCAGC 2 cut(s) 85, 107
BstXI CCANNNNNNTGG 1 cut(s) 242
BtsCI GGATG 2 cut(s) 336, 496
BtsIMutI CAGTG 1 cut(s) 474
Cfr13I GGNCC 1 cut(s) 418
CseI GACGC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 185
CspCI CAANNNNNGTGG 1 cut(s) 536
CviAII CATG 2 cut(s) 179, 455
CviJI RGCY 9 cut(s) 48, 98, 160, 167, 250, 289, 374, 401, 452
CviKI_1 RGCY 9 cut(s) 48, 98, 160, 167, 250, 289, 374, 401, 452
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 2 cut(s) 116, 156
DpnI GATC 4 cut(s) 348, 473, 488, 534
DpnII GATC 4 cut(s) 346, 471, 486, 532
DraI TTTAAA 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 291
FaeI CATG 2 cut(s) 182, 458
FaiI YATR 9 cut(s) 76, 78, 80, 180, 336, 371, 414, 456, 519
FaqI GGGAC 1 cut(s) 431
FatI CATG 2 cut(s) 178, 454
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 3 cut(s) 93, 96, 99
FokI GGATG 2 cut(s) 343, 483
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 3 cut(s) 93, 96, 99
GluI GCNGC 3 cut(s) 93, 96, 99
GsaI CCCAGC 1 cut(s) 52
HapII CCGG 2 cut(s) 174, 262
HgaI GACGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 182, 458
HinfI GANTC 3 cut(s) 35, 154, 458
HpaII CCGG 2 cut(s) 174, 262
HphI GGTGA 4 cut(s) 163, 356, 445, 542
Hpy188III TCNNGA 2 cut(s) 407, 536
Hpy99I CGWCG 2 cut(s) 92, 233
HpyAV CCTTC 1 cut(s) 510
HpyCH4V TGCA 2 cut(s) 383, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 380
HpyF3I CTNAG 2 cut(s) 116, 156
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 182, 458
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 4 cut(s) 346, 471, 486, 532
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 294
LpnPI CCDG 9 cut(s) 34, 134, 187, 228, 275, 327, 509, 536, 549
Lsp1109I GCAGC 2 cut(s) 85, 107
LweI GCATC 1 cut(s) 328
MaeIII GTNAC 1 cut(s) 151
MalI GATC 4 cut(s) 348, 473, 488, 534
MboI GATC 4 cut(s) 346, 471, 486, 532
MboII GAAGA 4 cut(s) 26, 197, 217, 268
MfeI CAATTG 2 cut(s) 440, 550
MhlI GDGCHC 2 cut(s) 291, 516
MluCI AATT 6 cut(s) 63, 221, 387, 440, 539, 550
MlyI GAGTC 3 cut(s) 44, 148, 452
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 5 cut(s) 116, 269, 298, 301, 424
MseI TTAA 2 cut(s) 62, 353
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 2 cut(s) 174, 262
MspR9I CCNGG 3 cut(s) 174, 263, 524
MunI CAATTG 2 cut(s) 440, 550
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 98, 380
NciI CCSGG 2 cut(s) 174, 263
NdeII GATC 4 cut(s) 346, 471, 486, 532
NlaIII CATG 2 cut(s) 182, 458
NlaIV GGNNCC 2 cut(s) 290, 419
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 151
NspI RCATGY 1 cut(s) 182
PciI ACATGT 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 210
PkrI GCNGC 3 cut(s) 94, 97, 100
PleI GAGTC 3 cut(s) 43, 148, 452
PpsI GAGTC 3 cut(s) 43, 148, 452
PscI ACATGT 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 48
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 2 cut(s) 290, 419
PspPI GGNCC 1 cut(s) 418
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 62, 353
SatI GCNGC 3 cut(s) 93, 96, 99
Sau3AI GATC 4 cut(s) 346, 471, 486, 532
Sau96I GGNCC 1 cut(s) 418
SchI GAGTC 3 cut(s) 44, 148, 452
ScrFI CCNGG 3 cut(s) 174, 263, 524
SduI GDGCHC 2 cut(s) 291, 516
SetI ASST 8 cut(s) 50, 100, 153, 162, 252, 435, 528, 532
SfaNI GCATC 1 cut(s) 328
SinI GGWCC 1 cut(s) 418
Sse9I AATT 6 cut(s) 63, 221, 387, 440, 539, 550
SsiI CCGC 3 cut(s) 58, 93, 130
StyD4I CCNGG 3 cut(s) 172, 261, 522
TaqI TCGA 4 cut(s) 38, 169, 228, 267
TasI AATT 6 cut(s) 63, 221, 387, 440, 539, 550
TatI WGTACW 1 cut(s) 184
TauI GCSGC 1 cut(s) 95
Tru1I TTAA 2 cut(s) 62, 353
Tru9I TTAA 2 cut(s) 62, 353
TscAI CASTG 1 cut(s) 481
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 2 cut(s) 95, 98
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 3 cut(s) 269, 367, 440
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 418
XceI RCATGY 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.