FvH4_1g11950

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001649
Physical Location & Seq
Forward (+)
6535949 .. 6539130
3182 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11950.t2

Sequence Viewer

Length: 1596 bp
ATGGAAGGAGAAGAAAAAGCTTATCACAGAGAGTCGAACACAAGCTGGTGTTGCAAATTATTTCTGTTACTCTATATAGCTCTGGTCGCCTCAGCTTCTACGTTCACCATTCTAAGAAACTTCAACCTGTACTACCATTCAAGCCGGGTCTCGGGTCGACCCGGAGGTATAGTGCAAAACTACTCTGACGCTCTTGGACTCGCCACGCAGTTCTTCGATGCCCAGAAATCTGGGAAGCTGGTGGTGAACCGGGTCGGGTGGAGAGGGGACTCGGGGCTCCGAGATGGGAGTGAGGAGGGGTTGGACTTGTCAAAGGGGATGTATGATGCTGGTGACCTTATAAAGTTTGGGTTTCCCATGGCTTTCACTGCAACAGTGTTGTCATGGTCTATTCTTGAATACGGTGAGCAAATGAAAAAGGTGAAGCAATTGGGTCATGCCCATGACTCTCTGAAGTGGATCACTGATTACTTGATCAATGCACATCCTTCCCCAAATGTGCTTTATATTCAGGTGGGTGATCCTGAGTTAGACCACAAATGTTGGCAAAGACCTGAAACCATGACAGAGAGGAGGCCTCTAGTTCAGATCAATACATCCTTCCCAGGAACAGAAGTGGCAGCAGAAACGGCAGCAGCTATGGCATCAGCATCTCTTGTTTTCAGGAAAATCGACTCCGCATACTCGAAATTGCTCCTCAAGCATGCCAAGCAGCTGTTTGTTTTTGCTGATACTTATAGAGGTTCTTATAGTATCAGCATACCCCAAGTGCAGCAGTTTTACAACTCTTCAGGATATGGAGATGAGCAGTTGTGGGCAGCTACCTGGCTCTACCATGCAACAGGAGAAGTGGACTATCTCAGGTATGTGACTGAACAGAACGGGCCGGCATTTGCCAATTGGGGAAGTCCATCTTGGTTTAGTTGGGATGACAAGCATGCTGCAACTCAGGTTTTGATGTCCAGGATAAGTTTCTTTGGTGCAAAAGACCTCTCTATGGCAGAGAACATTGATATACAGATGTACCGAAAAACTGCAGAGGTCTTCATGTGTGGACTTATACCTGATTCACCTACGGCCACATCTGATAGAACAGACAATGGTCTGATATGGGTGGAAGAATGGAACTGTCTGCAACATGCTTTGTCTTCAGCCTTTCTAGCAGTTCTCTATAGCGACTACATGGTTGCATCACAGACTGAAATGCTGTATTGTGATGGCAAAATTTACAATCCAGAAGATCTTCGTAGCTTCGCCATCTCTCAGGCAGATTACGCTTTGGGAAAAAACCCAATGAAGATGAGTTATCTTGTAGGGTATGGGAGTAACTACCCTCAACAAGTGCACCATAGGGGATCTTCAATTCCAGTTGATGCAGATACTGGCTGCAGCGATGGGTTTAAATGGCTGTACTCCCCCGATCCAAATCCGAATGTAGCTGTTGGTGCCCTGGTTGGTGGTCCTTTTCAGAATGATAGCTATATGGATATCAGGAACAATTCAATGCAAGGTGAACCAAGCACCTATAATAGTGCTCTCATTGTTGCCCTCCTCTCTGGCTTAGTTTCCACTTCTTCGGTGGCCATGCACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

532

Amino Acids

59.12

Weight (kDa)

5.4

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 61 - 321 7.4e-102 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 1445
AccI GTMKAC 1 cut(s) 157
AciI CCGC 1 cut(s) 678
AclWI GGATC 4 cut(s) 467, 515, 1363, 1415
AcoI YGGCCR 2 cut(s) 1077, 1581
AcsI RAATTY 1 cut(s) 1224
AcuI CTGAAG 3 cut(s) 473, 774, 1134
AfaI GTAC 3 cut(s) 131, 1025, 1412
AfiI CCNNNNNNNGG 2 cut(s) 151, 997
AgsI TTSAA 5 cut(s) 124, 141, 398, 1362, 1503
AjnI CCWGG 4 cut(s) 604, 824, 962, 1449
AjuI GAANNNNNNNTTGG 2 cut(s) 898, 930
Alw21I GWGCWC 2 cut(s) 1347, 1537
Alw26I GTCTC 1 cut(s) 154
Alw44I GTGCAC 1 cut(s) 1343
AlwI GGATC 4 cut(s) 467, 515, 1363, 1415
AlwNI CAGNNNCTG 1 cut(s) 1382
Ama87I CYCGRG 2 cut(s) 151, 271
AoxI GGCC 4 cut(s) 575, 884, 1077, 1581
ApaLI GTGCAC 1 cut(s) 1343
ApeKI GCWGC 9 cut(s) 620, 632, 635, 712, 772, 818, 941, 1386, 1389
ApoI RAATTY 1 cut(s) 1224
Asp700I GAANNNNTTC 1 cut(s) 1242
AspS9I GGNCC 2 cut(s) 884, 1460
AsuC2I CCSGG 3 cut(s) 146, 162, 251
AsuHPI GGTGA 8 cut(s) 97, 256, 344, 416, 433, 530, 1062, 1523
AvaI CYCGRG 2 cut(s) 151, 271
AvaII GGWCC 1 cut(s) 1460
BaeGI GKGCMC 2 cut(s) 1347, 1450
BaeI ACNNNNGTAYC 2 cut(s) 1007, 1040
BalI TGGCCA 1 cut(s) 1583
BanI GGYRCC 1 cut(s) 1445
BanII GRGCYC 1 cut(s) 279
BbsI GAAGAC 2 cut(s) 1036, 1140
Bbv12I GWGCWC 2 cut(s) 1347, 1537
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 9 cut(s) 632, 644, 647, 724, 784, 830, 928, 1373, 1401
BccI CCATC 5 cut(s) 278, 919, 1211, 1265, 1388
BceAI ACGGC 2 cut(s) 645, 1092
BciT130I CCWGG 4 cut(s) 606, 826, 964, 1451
BclI TGATCA 1 cut(s) 474
BcnI CCSGG 3 cut(s) 146, 162, 251
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 2 cut(s) 581, 1160
BfmI CTRYAG 3 cut(s) 1035, 1171, 1387
BglII AGATCT 1 cut(s) 1240
BisI GCNGC 9 cut(s) 621, 633, 636, 713, 773, 819, 942, 1387, 1390
BlsI GCNGC 9 cut(s) 622, 634, 637, 714, 774, 820, 943, 1388, 1391
Bme1390I CCNGG 7 cut(s) 146, 162, 251, 606, 826, 964, 1451
Bme18I GGWCC 1 cut(s) 1460
BmeT110I CYCGRG 2 cut(s) 151, 271
BmgT120I GGNCC 2 cut(s) 884, 1460
BmiI GGNNCC 2 cut(s) 278, 1447
BmrFI CCNGG 7 cut(s) 146, 162, 251, 606, 826, 964, 1451
BmsI GCATC 6 cut(s) 208, 316, 653, 659, 1199, 1363
BoxI GACNNNNGTC 1 cut(s) 1101
BpiI GAAGAC 2 cut(s) 1036, 1140
BplI GAGNNNNNCTC 2 cut(s) 562, 594
Bpu10I CCTNAGC 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 3 cut(s) 146, 162, 251
BsaBI GATNNNNATC 1 cut(s) 1425
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 3 cut(s) 357, 604, 1449
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 997
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 2 cut(s) 1367, 1387
Bse8I GATNNNNATC 1 cut(s) 1425
BseBI CCWGG 4 cut(s) 606, 826, 964, 1451
BseDI CCNNGG 3 cut(s) 357, 604, 1449
BseGI GGATG 4 cut(s) 324, 484, 596, 934
BseJI GATNNNNATC 1 cut(s) 1425
BseLI CCNNNNNNNGG 2 cut(s) 151, 997
BseMII CTCAG 5 cut(s) 105, 516, 874, 962, 1277
BseNI ACTGG 2 cut(s) 1367, 1387
BseRI GAGGAG 4 cut(s) 308, 586, 686, 1541
BseSI GKGCMC 2 cut(s) 1347, 1450
BseXI GCAGC 9 cut(s) 632, 644, 647, 724, 784, 830, 928, 1373, 1401
BsgI GTGCAG 1 cut(s) 791
BshFI GGCC 4 cut(s) 577, 886, 1079, 1583
BshNI GGYRCC 1 cut(s) 1445
BsiHKAI GWGCWC 2 cut(s) 1347, 1537
BsiHKCI CYCGRG 2 cut(s) 151, 271
BsiSI CCGG 4 cut(s) 145, 162, 250, 887
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 151, 997
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 4 cut(s) 577, 886, 1079, 1583
Bso31I GGTCTC 1 cut(s) 154
BsoBI CYCGRG 2 cut(s) 151, 271
Bsp1286I GDGCHC 4 cut(s) 279, 1347, 1450, 1537
Bsp143I GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 1 cut(s) 678
BspANI GGCC 4 cut(s) 577, 886, 1079, 1583
BspCNI CTCAG 5 cut(s) 104, 517, 873, 961, 1276
BspLI GGNNCC 2 cut(s) 278, 1447
BspMAI CTGCAG 2 cut(s) 1039, 1391
BspPI GGATC 4 cut(s) 467, 515, 1363, 1415
BspT107I GGYRCC 1 cut(s) 1445
BspTNI GGTCTC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 2 cut(s) 1367, 1387
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 3 cut(s) 357, 604, 1449
BssMI GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 4 cut(s) 606, 826, 964, 1451
Bst4CI ACNGT 3 cut(s) 376, 404, 1130
Bst6I CTCTTC 1 cut(s) 793
BstC8I GCNNGC 3 cut(s) 705, 888, 939
BstDEI CTNAG 7 cut(s) 91, 113, 525, 860, 948, 1263, 1561
BstDSI CCRYGG 1 cut(s) 357
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 4 cut(s) 324, 484, 596, 934
BstKTI GATC 7 cut(s) 462, 477, 523, 591, 1243, 1358, 1423
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
BstNI CCWGG 4 cut(s) 606, 826, 964, 1451
BstNSI RCATGY 3 cut(s) 707, 941, 1142
BstPAI GACNNNNGTC 1 cut(s) 1101
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 7 cut(s) 144, 160, 249, 604, 824, 962, 1449
BstSFI CTRYAG 3 cut(s) 1035, 1171, 1387
BstSLI GKGCMC 2 cut(s) 1347, 1450
BstV1I GCAGC 9 cut(s) 632, 644, 647, 724, 784, 830, 928, 1373, 1401
BstV2I GAAGAC 2 cut(s) 1036, 1140
BstX2I RGATCY 2 cut(s) 1240, 1355
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 2 cut(s) 1240, 1355
BsuRI GGCC 4 cut(s) 577, 886, 1079, 1583
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 1407
BtsCI GGATG 4 cut(s) 324, 484, 596, 934
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 366, 381, 462
Cac8I GCNNGC 3 cut(s) 705, 888, 939
CaiI CAGNNNCTG 1 cut(s) 1382
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 2 cut(s) 884, 1460
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 130, 1024, 1411
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
CviQI GTAC 3 cut(s) 130, 1024, 1411
DdeI CTNAG 7 cut(s) 91, 113, 525, 860, 948, 1263, 1561
DpnI GATC 7 cut(s) 461, 476, 522, 590, 1242, 1357, 1422
DpnII GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
DraI TTTAAA 1 cut(s) 1402
EaeI YGGCCR 2 cut(s) 1077, 1581
Eam1104I CTCTTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 793
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 577
Eco24I GRGCYC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 1489
Eco47I GGWCC 1 cut(s) 1460
Eco57I CTGAAG 3 cut(s) 473, 774, 1134
Eco88I CYCGRG 2 cut(s) 151, 271
Eco91I GGTNACC 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 4 cut(s) 604, 824, 962, 1449
EcoRV GATATC 1 cut(s) 1489
EcoT14I CCWWGG 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 357
FalI AAGNNNNNCTT 2 cut(s) 898, 930
FaqI GGGAC 1 cut(s) 281
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 9 cut(s) 621, 633, 636, 713, 773, 819, 942, 1387, 1390
FokI GGATG 4 cut(s) 331, 471, 583, 941
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 9 cut(s) 621, 633, 636, 713, 773, 819, 942, 1387, 1390
FspBI CTAG 2 cut(s) 581, 1160
GluI GCNGC 9 cut(s) 621, 633, 636, 713, 773, 819, 942, 1387, 1390
HaeIII GGCC 4 cut(s) 577, 886, 1079, 1583
HapII CCGG 4 cut(s) 145, 162, 250, 887
HgaI GACGC 1 cut(s) 197
HincII GTYRAC 1 cut(s) 158
HindII GTYRAC 1 cut(s) 158
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 6 cut(s) 32, 198, 269, 446, 674, 1067
HpaII CCGG 4 cut(s) 145, 162, 250, 887
HphI GGTGA 8 cut(s) 97, 256, 344, 416, 433, 530, 1062, 1523
Hpy166II GTNNAC 7 cut(s) 105, 158, 247, 853, 1055, 1345, 1514
Hpy188I TCNGA 8 cut(s) 187, 281, 453, 588, 1087, 1107, 1431, 1470
Hpy188III TCNNGA 6 cut(s) 395, 524, 664, 792, 1235, 1492
Hpy8I GTNNAC 7 cut(s) 105, 158, 247, 853, 1055, 1345, 1514
HpyAV CCTTC 2 cut(s) 498, 610
HpyCH4III ACNGT 3 cut(s) 376, 404, 1130
HpyCH4IV ACGT 1 cut(s) 101
HpyF3I CTNAG 7 cut(s) 91, 113, 525, 860, 948, 1263, 1561
HpySE526I ACGT 1 cut(s) 101
KroI GCCGGC 1 cut(s) 886
KroNI GCCGGC 1 cut(s) 888
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
LmnI GCTCC 2 cut(s) 282, 699
Lsp1109I GCAGC 9 cut(s) 632, 644, 647, 724, 784, 830, 928, 1373, 1401
LweI GCATC 6 cut(s) 208, 316, 653, 659, 1199, 1363
MaeI CTAG 2 cut(s) 581, 1160
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 4 cut(s) 66, 332, 868, 1325
MalI GATC 7 cut(s) 461, 476, 522, 590, 1242, 1357, 1422
MboI GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
MfeI CAATTG 2 cut(s) 428, 898
MflI RGATCY 2 cut(s) 1240, 1355
MhlI GDGCHC 4 cut(s) 279, 1347, 1450, 1537
MlsI TGGCCA 1 cut(s) 1583
MluCI AATT 7 cut(s) 56, 428, 689, 898, 1224, 1362, 1498
MluNI TGGCCA 1 cut(s) 1583
MlyI GAGTC 5 cut(s) 41, 192, 263, 440, 668
MmeI TCCRAC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 1583
MroNI GCCGGC 1 cut(s) 886
MroXI GAANNNNTTC 1 cut(s) 1242
MscI TGGCCA 1 cut(s) 1583
MseI TTAA 1 cut(s) 1401
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 1 cut(s) 1583
MspA1I CMGCKG 1 cut(s) 715
MspI CCGG 4 cut(s) 145, 162, 250, 887
MspR9I CCNGG 7 cut(s) 146, 162, 251, 606, 826, 964, 1451
MunI CAATTG 2 cut(s) 428, 898
MvaI CCWGG 4 cut(s) 606, 826, 964, 1451
NaeI GCCGGC 1 cut(s) 888
NciI CCSGG 3 cut(s) 146, 162, 251
NcoI CCATGG 1 cut(s) 357
NdeII GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
NgoMIV GCCGGC 1 cut(s) 886
NlaIV GGNNCC 2 cut(s) 278, 1447
NmuCI GTSAC 2 cut(s) 332, 868
NspI RCATGY 3 cut(s) 707, 941, 1142
PaeI GCATGC 2 cut(s) 707, 941
PceI AGGCCT 1 cut(s) 577
PdiI GCCGGC 1 cut(s) 888
PdmI GAANNNNTTC 1 cut(s) 1242
PfeI GAWTC 1 cut(s) 1067
PfoI TCCNGGA 1 cut(s) 962
PkrI GCNGC 9 cut(s) 622, 634, 637, 714, 774, 820, 943, 1388, 1391
PleI GAGTC 5 cut(s) 40, 192, 263, 440, 668
PpsI GAGTC 5 cut(s) 40, 192, 263, 440, 668
PshAI GACNNNNGTC 1 cut(s) 1101
PsiI TTATAA 1 cut(s) 341
Psp6I CCWGG 4 cut(s) 604, 824, 962, 1449
PspEI GGTNACC 1 cut(s) 332
PspGI CCWGG 4 cut(s) 604, 824, 962, 1449
PspN4I GGNNCC 2 cut(s) 278, 1447
PspPI GGNCC 2 cut(s) 884, 1460
PstI CTGCAG 2 cut(s) 1039, 1391
PstNI CAGNNNCTG 1 cut(s) 1382
PsuI RGATCY 2 cut(s) 1240, 1355
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 3 cut(s) 131, 1025, 1412
RsaNI GTAC 3 cut(s) 130, 1024, 1411
RseI CAYNNNNRTG 1 cut(s) 441
SalI GTCGAC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 1401
SatI GCNGC 9 cut(s) 621, 633, 636, 713, 773, 819, 942, 1387, 1390
Sau3AI GATC 7 cut(s) 459, 474, 520, 588, 1240, 1355, 1420
Sau96I GGNCC 2 cut(s) 884, 1460
SchI GAGTC 5 cut(s) 41, 192, 263, 440, 668
ScrFI CCNGG 7 cut(s) 146, 162, 251, 606, 826, 964, 1451
SduI GDGCHC 4 cut(s) 279, 1347, 1450, 1537
SfaNI GCATC 6 cut(s) 208, 316, 653, 659, 1199, 1363
SfcI CTRYAG 3 cut(s) 1035, 1171, 1387
SinI GGWCC 1 cut(s) 1460
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SphI GCATGC 2 cut(s) 707, 941
Sse9I AATT 7 cut(s) 56, 428, 689, 898, 1224, 1362, 1498
SseBI AGGCCT 1 cut(s) 577
SsiI CCGC 1 cut(s) 678
SspMI CTAG 2 cut(s) 581, 1160
StuI AGGCCT 1 cut(s) 577
StyD4I CCNGG 7 cut(s) 144, 160, 249, 604, 824, 962, 1449
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 3 cut(s) 376, 404, 1130
TaiI ACGT 1 cut(s) 104
TaqI TCGA 5 cut(s) 35, 157, 216, 672, 686
TasI AATT 7 cut(s) 56, 428, 689, 898, 1224, 1362, 1498
TatI WGTACW 2 cut(s) 129, 1410
TfiI GAWTC 1 cut(s) 1067
Tru1I TTAA 1 cut(s) 1401
Tru9I TTAA 1 cut(s) 1401
TscAI CASTG 3 cut(s) 373, 381, 469
TseFI GTSAC 2 cut(s) 332, 868
TseI GCWGC 9 cut(s) 620, 632, 635, 712, 772, 818, 941, 1386, 1389
Tsp45I GTSAC 2 cut(s) 332, 868
TspDTI ATGAA 3 cut(s) 428, 1036, 1310
TspRI CASTG 3 cut(s) 373, 381, 469
VneI GTGCAC 1 cut(s) 1343
VpaK11BI GGWCC 1 cut(s) 1460
XapI RAATTY 1 cut(s) 1224
XceI RCATGY 3 cut(s) 707, 941, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 1576
XmiI GTMKAC 1 cut(s) 157
XmnI GAANNNNTTC 1 cut(s) 1242
XspI CTAG 2 cut(s) 581, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.