Rh2DG134900

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001649
Physical Location & Seq
Forward (+)
11361373 .. 11363417
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG134900.1

Sequence Viewer

Length: 1356 bp
ATGAAGAACCGGGTCGCGTGGAGAGGCGACTCGGGGCTCCAAGATGGGAGTGAGGAGGGGTTAGACTTATCAAAGGGGATGTATGATGCTGGTGATCTTTTAAAGTTTGGGTTTCCTATGGCTTTCACTGCAACAGTTTTGTCTTGGGCTATTCTTGAATATGGGGACCAAATGAAAGAGGTGAAGCAATTGGGTCATGCCCATGACTCGCTGAAGTGGATCACTGATTACTTGATCAATGCACATCCTTCCCCAAATGTGCTCTATATCCAGGTGGGTGATCCTGAATTAGACCACAATTGTTGGCAAAGACCTGAAACCATGACAGAGAGAAGGCCCCTAGTTAAAATCAACACATCTTTCCCAGGAACAGAAGTGGCAGCAGAAACAGCAGCAGCTATGGCATCAGCATCTCTTGTTTTCAGGAAAATCGACTCCTCATACTCAAAATCGCTCCTCAAGCATGCCGAGCAGCTGTTTAATTTTGCCGATACTTATAGAGGTTCTTACAGTGCCAGCATACCCCAAGTGCAGGGGTTCTACAACTCTTCAGGATATGCAGATGAGCTATTGTGGGCAGCTACCTGGCTCTACCATGCAACTGGAGATCTGGACTACCTCAAGTATGTGACCGAACAGAACGGGCCAGCATTTGCTAACTGGGGAAGTCCATCTTGGTTTAGTTGGGATGACAAGCATGCTGCAACTCAAGTTTTGATGTCCAGGATAAGTTTCTTCGGTGCAAAAGAGCTCCTTATGGCAGAGAACCTTGATCTCCAGATGTACCGAAAAACTGCAGAGGTCTTCTTGTGTGGACTTATTCCTGATTCGCCTACGGCCGCACCTGACAGAACAGACAATGGTCTGATATGGGTGGAAGAATGGAACTGTCTACAACATGCTATGTCTTCAGCCTTTCTAGCAGTTCTCTATAGCGACTACATGGTTACGTCTCAGACTGAAATGCTGTATTGTGATGGGAAAATTTACAAGCCCGAAGATCTTCGTAGCTTCTCCATCTCTCAGGCAGATTATGCGTTGGGAAAAAATCCGATGAAGATGAGTTATCTTGTAGGGTATGGGGGTAACTACCCTCAACAAGTGCACCATAGGGGATCTTCAATTCCTGTTGATGCAGACACTGGCTGCAGAGATGGGTTTAAATGGCTGTACTCCCCCGATCCGAATCCAAATGTAGCTGTTGGTGCCCTGGTTGGTGGCCCTTTTCAGAATGATAGCTATATGGATATCAGGAACAATTCAAAGCAAGGTGAACCAAGCACTTACAATAGTGCTCTCATTGTTGCCCTACTCTCTGGCTTAGTTTCCACTTCTTCGGTGCCCAAGCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

451

Amino Acids

50.16

Weight (kDa)

5.1

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 3 - 438 1.5e-150 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1354
AccB1I GGYRCC 2 cut(s) 1205, 1339
AccI GTMKAC 1 cut(s) 892
AccII CGCG 1 cut(s) 17
AciI CCGC 1 cut(s) 840
AclWI GGATC 4 cut(s) 227, 275, 1123, 1175
AcoI YGGCCR 1 cut(s) 837
AcsI RAATTY 1 cut(s) 984
AcuI CTGAAG 3 cut(s) 233, 534, 894
AfaI GTAC 2 cut(s) 785, 1172
AfiI CCNNNNNNNGG 1 cut(s) 532
AgsI TTSAA 3 cut(s) 158, 1122, 1263
AjnI CCWGG 5 cut(s) 270, 364, 584, 722, 1209
AjuI GAANNNNNNNTTGG 2 cut(s) 658, 690
AluBI AGCT 8 cut(s) 398, 475, 568, 581, 751, 1011, 1199, 1239
AluI AGCT 8 cut(s) 398, 475, 568, 581, 751, 1011, 1199, 1239
Alw21I GWGCWC 4 cut(s) 264, 753, 1107, 1297
Alw26I GTCTC 1 cut(s) 957
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 4 cut(s) 227, 275, 1123, 1175
AlwNI CAGNNNCTG 1 cut(s) 1142
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 4 cut(s) 335, 644, 837, 1219
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 7 cut(s) 380, 392, 395, 472, 578, 701, 1146
ApoI RAATTY 1 cut(s) 984
Asp700I GAANNNNTTC 1 cut(s) 1002
AspS9I GGNCC 4 cut(s) 166, 336, 644, 1220
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 4 cut(s) 104, 193, 290, 1283
AvaI CYCGRG 1 cut(s) 31
AvaII GGWCC 1 cut(s) 166
BaeGI GKGCMC 3 cut(s) 1107, 1210, 1344
BanI GGYRCC 2 cut(s) 1205, 1339
BanII GRGCYC 2 cut(s) 39, 753
BbsI GAAGAC 2 cut(s) 796, 900
Bbv12I GWGCWC 4 cut(s) 264, 753, 1107, 1297
BbvI GCAGC 7 cut(s) 392, 404, 407, 484, 590, 688, 1133
BccI CCATC 5 cut(s) 38, 679, 971, 1025, 1148
BceAI ACGGC 1 cut(s) 852
BciT130I CCWGG 5 cut(s) 272, 366, 586, 724, 1211
BclI TGATCA 1 cut(s) 234
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 957
BfaI CTAG 2 cut(s) 341, 920
BfmI CTRYAG 3 cut(s) 795, 931, 1147
BglII AGATCT 2 cut(s) 607, 1000
BisI GCNGC 8 cut(s) 381, 393, 396, 473, 579, 702, 840, 1147
BlsI GCNGC 8 cut(s) 382, 394, 397, 474, 580, 703, 841, 1148
Bme1390I CCNGG 6 cut(s) 11, 272, 366, 586, 724, 1211
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 4 cut(s) 166, 336, 644, 1220
BmiI GGNNCC 5 cut(s) 38, 167, 338, 1207, 1341
BmrFI CCNGG 6 cut(s) 11, 272, 366, 586, 724, 1211
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 4 cut(s) 76, 413, 419, 1123
BmuI ACTGGG 1 cut(s) 670
BoxI GACNNNNGTC 1 cut(s) 861
BpiI GAAGAC 2 cut(s) 796, 900
BpmI CTGGAG 2 cut(s) 624, 761
BpuEI CTTGAG 3 cut(s) 443, 605, 693
BpuMI CCSGG 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 1185
BsaJI CCNNGG 2 cut(s) 364, 1209
Bsc4I CCNNNNNNNGG 1 cut(s) 532
Bse1I ACTGG 3 cut(s) 607, 665, 1147
Bse8I GATNNNNATC 1 cut(s) 1185
BseBI CCWGG 5 cut(s) 272, 366, 586, 724, 1211
BseDI CCNNGG 2 cut(s) 364, 1209
BseGI GGATG 3 cut(s) 84, 244, 694
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 1 cut(s) 532
BseMII CTCAG 2 cut(s) 968, 1037
BseNI ACTGG 3 cut(s) 607, 665, 1147
BseRI GAGGAG 3 cut(s) 68, 427, 446
BseSI GKGCMC 3 cut(s) 1107, 1210, 1344
BseX3I CGGCCG 1 cut(s) 837
BseXI GCAGC 7 cut(s) 392, 404, 407, 484, 590, 688, 1133
BsgI GTGCAG 1 cut(s) 551
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 840
BshFI GGCC 4 cut(s) 337, 646, 839, 1221
BshNI GGYRCC 2 cut(s) 1205, 1339
BsiEI CGRYCG 1 cut(s) 840
BsiHKAI GWGCWC 4 cut(s) 264, 753, 1107, 1297
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 532
BsmAI GTCTC 1 cut(s) 957
BsmBI CGTCTC 1 cut(s) 957
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 4 cut(s) 337, 646, 839, 1221
BsoBI CYCGRG 1 cut(s) 31
Bsp1286I GDGCHC 7 cut(s) 39, 264, 753, 1107, 1210, 1297, 1344
Bsp143I GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
BspACI CCGC 1 cut(s) 840
BspANI GGCC 4 cut(s) 337, 646, 839, 1221
BspCNI CTCAG 2 cut(s) 967, 1036
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 5 cut(s) 38, 167, 338, 1207, 1341
BspMAI CTGCAG 2 cut(s) 799, 1151
BspPI GGATC 4 cut(s) 227, 275, 1123, 1175
BspT107I GGYRCC 2 cut(s) 1205, 1339
BsrI ACTGG 3 cut(s) 607, 665, 1147
BssECI CCNNGG 2 cut(s) 364, 1209
BssMI GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
Bst2UI CCWGG 5 cut(s) 272, 366, 586, 724, 1211
Bst4CI ACNGT 3 cut(s) 136, 512, 890
Bst6I CTCTTC 1 cut(s) 553
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstC8I GCNNGC 4 cut(s) 465, 517, 648, 699
BstDEI CTNAG 3 cut(s) 954, 1023, 1321
BstF5I GGATG 3 cut(s) 84, 244, 694
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 9 cut(s) 97, 222, 237, 283, 610, 775, 1003, 1118, 1183
BstMAI GTCTC 1 cut(s) 957
BstMBI GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
BstMCI CGRYCG 1 cut(s) 840
BstMWI GCNNNNNNNGC 8 cut(s) 128, 389, 401, 460, 469, 920, 1034, 1205
BstNI CCWGG 5 cut(s) 272, 366, 586, 724, 1211
BstNSI RCATGY 3 cut(s) 467, 701, 902
BstPAI GACNNNNGTC 1 cut(s) 861
BstSCI CCNGG 6 cut(s) 9, 270, 364, 584, 722, 1209
BstSFI CTRYAG 3 cut(s) 795, 931, 1147
BstSLI GKGCMC 3 cut(s) 1107, 1210, 1344
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 7 cut(s) 392, 404, 407, 484, 590, 688, 1133
BstV2I GAAGAC 2 cut(s) 796, 900
BstX2I RGATCY 3 cut(s) 607, 1000, 1115
BstXI CCANNNNNNTGG 1 cut(s) 602
BstYI RGATCY 3 cut(s) 607, 1000, 1115
BstZI CGGCCG 1 cut(s) 837
BsuRI GGCC 4 cut(s) 337, 646, 839, 1221
BtsCI GGATG 3 cut(s) 84, 244, 694
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 4 cut(s) 126, 222, 517, 1140
Cac8I GCNNGC 4 cut(s) 465, 517, 648, 699
CaiI CAGNNNCTG 1 cut(s) 1142
Cfr13I GGNCC 4 cut(s) 166, 336, 644, 1220
Csp6I GTAC 2 cut(s) 784, 1171
CspCI CAANNNNNGTGG 2 cut(s) 284, 319
CviAII CATG 8 cut(s) 197, 203, 322, 464, 596, 698, 899, 943
CviQI GTAC 2 cut(s) 784, 1171
DdeI CTNAG 3 cut(s) 954, 1023, 1321
DpnI GATC 9 cut(s) 96, 221, 236, 282, 609, 774, 1002, 1117, 1182
DpnII GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
DraI TTTAAA 2 cut(s) 102, 1162
EaeI YGGCCR 1 cut(s) 837
EagI CGGCCG 1 cut(s) 837
Eam1104I CTCTTC 1 cut(s) 553
EarI CTCTTC 1 cut(s) 553
Ecl136II GAGCTC 1 cut(s) 751
EclXI CGGCCG 1 cut(s) 837
Eco24I GRGCYC 2 cut(s) 39, 753
Eco32I GATATC 1 cut(s) 1249
Eco47I GGWCC 1 cut(s) 166
Eco52I CGGCCG 1 cut(s) 837
Eco53kI GAGCTC 1 cut(s) 751
Eco57I CTGAAG 3 cut(s) 233, 534, 894
Eco88I CYCGRG 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 751
EcoO109I RGGNCCY 1 cut(s) 336
EcoRII CCWGG 5 cut(s) 270, 364, 584, 722, 1209
EcoRV GATATC 1 cut(s) 1249
EcoT38I GRGCYC 2 cut(s) 39, 753
Esp3I CGTCTC 1 cut(s) 957
FaeI CATG 8 cut(s) 200, 206, 325, 467, 599, 701, 902, 946
FalI AAGNNNNNCTT 4 cut(s) 658, 690, 738, 770
FaqI GGGAC 1 cut(s) 179
FatI CATG 8 cut(s) 196, 202, 321, 463, 595, 697, 898, 942
FbaI TGATCA 1 cut(s) 234
FblI GTMKAC 1 cut(s) 892
Fnu4HI GCNGC 8 cut(s) 381, 393, 396, 473, 579, 702, 840, 1147
FokI GGATG 3 cut(s) 91, 231, 701
FriOI GRGCYC 2 cut(s) 39, 753
Fsp4HI GCNGC 8 cut(s) 381, 393, 396, 473, 579, 702, 840, 1147
FspBI CTAG 2 cut(s) 341, 920
GluI GCNGC 8 cut(s) 381, 393, 396, 473, 579, 702, 840, 1147
GsuI CTGGAG 2 cut(s) 624, 761
HaeIII GGCC 4 cut(s) 337, 646, 839, 1221
HapII CCGG 1 cut(s) 10
Hin1II CATG 8 cut(s) 200, 206, 325, 467, 599, 701, 902, 946
HinfI GANTC 5 cut(s) 29, 206, 434, 827, 1186
HpaII CCGG 1 cut(s) 10
HphI GGTGA 4 cut(s) 104, 193, 290, 1283
Hpy166II GTNNAC 4 cut(s) 815, 893, 1105, 1274
Hpy188I TCNGA 5 cut(s) 867, 957, 1053, 1185, 1230
Hpy188III TCNNGA 8 cut(s) 155, 284, 424, 552, 611, 778, 824, 1252
Hpy8I GTNNAC 4 cut(s) 815, 893, 1105, 1274
HpyAV CCTTC 2 cut(s) 258, 327
HpyCH4III ACNGT 3 cut(s) 136, 512, 890
HpyCH4IV ACGT 1 cut(s) 950
HpyF10VI GCNNNNNNNGC 8 cut(s) 128, 389, 401, 460, 469, 920, 1034, 1205
HpyF3I CTNAG 3 cut(s) 954, 1023, 1321
HpySE526I ACGT 1 cut(s) 950
Hsp92II CATG 8 cut(s) 200, 206, 325, 467, 599, 701, 902, 946
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
LmnI GCTCC 3 cut(s) 42, 459, 756
Lsp1109I GCAGC 7 cut(s) 392, 404, 407, 484, 590, 688, 1133
LweI GCATC 4 cut(s) 76, 413, 419, 1123
MaeI CTAG 2 cut(s) 341, 920
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 3 cut(s) 628, 946, 1085
MalI GATC 9 cut(s) 96, 221, 236, 282, 609, 774, 1002, 1117, 1182
MboI GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
MfeI CAATTG 2 cut(s) 188, 298
MflI RGATCY 3 cut(s) 607, 1000, 1115
MhlI GDGCHC 7 cut(s) 39, 264, 753, 1107, 1210, 1297, 1344
MluCI AATT 7 cut(s) 188, 287, 298, 481, 984, 1122, 1258
MlyI GAGTC 3 cut(s) 23, 200, 428
MroXI GAANNNNTTC 1 cut(s) 1002
MseI TTAA 4 cut(s) 101, 345, 480, 1161
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 6 cut(s) 11, 272, 366, 586, 724, 1211
MunI CAATTG 2 cut(s) 188, 298
MvaI CCWGG 5 cut(s) 272, 366, 586, 724, 1211
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 8 cut(s) 128, 389, 401, 460, 469, 920, 1034, 1205
NciI CCSGG 1 cut(s) 11
NdeII GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
NlaIII CATG 8 cut(s) 200, 206, 325, 467, 599, 701, 902, 946
NlaIV GGNNCC 5 cut(s) 38, 167, 338, 1207, 1341
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 628
NspI RCATGY 3 cut(s) 467, 701, 902
PaeI GCATGC 2 cut(s) 467, 701
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 2 cut(s) 827, 1186
PfoI TCCNGGA 1 cut(s) 722
PkrI GCNGC 8 cut(s) 382, 394, 397, 474, 580, 703, 841, 1148
PleI GAGTC 3 cut(s) 23, 200, 428
PpsI GAGTC 3 cut(s) 23, 200, 428
PshAI GACNNNNGTC 1 cut(s) 861
PsiI TTATAA 1 cut(s) 1354
Psp124BI GAGCTC 1 cut(s) 753
Psp6I CCWGG 5 cut(s) 270, 364, 584, 722, 1209
PspGI CCWGG 5 cut(s) 270, 364, 584, 722, 1209
PspN4I GGNNCC 5 cut(s) 38, 167, 338, 1207, 1341
PspPI GGNCC 4 cut(s) 166, 336, 644, 1220
PstI CTGCAG 2 cut(s) 799, 1151
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 3 cut(s) 607, 1000, 1115
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 2 cut(s) 785, 1172
RsaNI GTAC 2 cut(s) 784, 1171
RseI CAYNNNNRTG 1 cut(s) 201
SacI GAGCTC 1 cut(s) 753
SaqAI TTAA 4 cut(s) 101, 345, 480, 1161
SatI GCNGC 8 cut(s) 381, 393, 396, 473, 579, 702, 840, 1147
Sau3AI GATC 9 cut(s) 94, 219, 234, 280, 607, 772, 1000, 1115, 1180
Sau96I GGNCC 4 cut(s) 166, 336, 644, 1220
SchI GAGTC 3 cut(s) 23, 200, 428
ScrFI CCNGG 6 cut(s) 11, 272, 366, 586, 724, 1211
SduI GDGCHC 7 cut(s) 39, 264, 753, 1107, 1210, 1297, 1344
SfaNI GCATC 4 cut(s) 76, 413, 419, 1123
SfcI CTRYAG 3 cut(s) 795, 931, 1147
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 201
SmlI CTYRAG 3 cut(s) 458, 620, 708
SmoI CTYRAG 3 cut(s) 458, 620, 708
SphI GCATGC 2 cut(s) 467, 701
Sse9I AATT 7 cut(s) 188, 287, 298, 481, 984, 1122, 1258
SsiI CCGC 1 cut(s) 840
SspMI CTAG 2 cut(s) 341, 920
SstI GAGCTC 1 cut(s) 753
StyD4I CCNGG 6 cut(s) 9, 270, 364, 584, 722, 1209
TaaI ACNGT 3 cut(s) 136, 512, 890
TaiI ACGT 1 cut(s) 953
TaqI TCGA 1 cut(s) 432
TaqII GACCGA 1 cut(s) 647
TasI AATT 7 cut(s) 188, 287, 298, 481, 984, 1122, 1258
TatI WGTACW 1 cut(s) 1170
TauI GCSGC 1 cut(s) 842
TfiI GAWTC 2 cut(s) 827, 1186
Tru1I TTAA 4 cut(s) 101, 345, 480, 1161
Tru9I TTAA 4 cut(s) 101, 345, 480, 1161
TscAI CASTG 4 cut(s) 133, 229, 517, 1147
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 7 cut(s) 380, 392, 395, 472, 578, 701, 1146
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 3 cut(s) 17, 188, 1070
TspRI CASTG 4 cut(s) 133, 229, 517, 1147
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 1 cut(s) 984
XceI RCATGY 3 cut(s) 467, 701, 902
XmiI GTMKAC 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 2 cut(s) 341, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.