RchiOBHm_Chr2g0099561

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001649
Physical Location & Seq
Forward (+)
11736841 .. 11738239
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47428

Sequence Viewer

Length: 921 bp
ATGACAGAGAGAAGGCCTCTAGTTCAAATCAACACATCTTTCCCAGGAACAGAAGTGGCAGCAGAAACAGCAGCAACTATAGCATCAACATATCTTGTTTTCAGGAAAATTGACTCCTCATACTCAAAATCGCTCCTCAAGCATGTCGAGCAGCTATTTAATTTTGCCGATACTTATAGAGGTTCTTATAGTGCCAGCATACCCCAAGTGCAGGGGTTCTATAACTCTTCAGGATATGCAGATGAGCTATTGTGGGCAGCTACCTGGCTCTACCATGCAACTGGAGATCTGGACTACCTCAAGTATGTGACCGAACAGAACGGGTCGGCATTTGCTAACTGGGGAAGTCCATCTTGGTTTAGTTGGGATGACAAGCATGCTGCAACTAAAGTAAATTTAGTTCTTAATGTCCAATCATGTCAAAATGGTCTGATATGGGTGGAAGAATGGAACTGTCTACAACATGCTATGTCTTCAGCCTTTCTAGCAGTTCTCTATAGCGACTACATGGTTACGTCTCAGACTGAAATGCTGTATTGTGATGGGAAAATTTACAAGCCCGAAGATCTTCGTAGCTTCTCCATCTCTCAGGCAGATTATGCGTTGGGAAAAAATCCGATGAAGATGAGTTATCTTGTAGGGTATGGGGGTAACTACCCTCAACAAGTGCACCATAGGGGATCTTCAATTCCTGTTGATGCAGACACTGGCTGCAGAGATGGGTTTAAATGGCTATACTCCCCCGATCCGAATCCAAATGTAGCTGTTGGTGCCCTGGTTGGTGGCCCTTTTCAGAATGATAGCTATATGGATATCAGGAACAATTCAAAGCAAGGTGAACCAAGCACTTACAATAGTGCTCTCATTGTTGCCCTACTCTCTGGCTTAGTTTCCACTTCTTCGGTGCCCAAGCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

306

Amino Acids

33.98

Weight (kDa)

5.46

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 293 1.4e-82 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 919
AccB1I GGYRCC 2 cut(s) 770, 904
AccI GTMKAC 1 cut(s) 457
AclWI GGATC 2 cut(s) 688, 740
AcsI RAATTY 2 cut(s) 394, 549
AcuI CTGAAG 2 cut(s) 213, 459
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 3 cut(s) 26, 687, 828
AjnI CCWGG 3 cut(s) 43, 263, 774
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 6 cut(s) 154, 247, 260, 576, 764, 804
AluI AGCT 6 cut(s) 154, 247, 260, 576, 764, 804
Alw21I GWGCWC 2 cut(s) 672, 862
Alw26I GTCTC 1 cut(s) 522
Alw44I GTGCAC 1 cut(s) 668
AlwI GGATC 2 cut(s) 688, 740
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 2 cut(s) 14, 784
ApaLI GTGCAC 1 cut(s) 668
ApeKI GCWGC 6 cut(s) 59, 71, 151, 257, 380, 711
ApoI RAATTY 2 cut(s) 394, 549
Asp700I GAANNNNTTC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 785
AsuHPI GGTGA 1 cut(s) 848
BaeGI GKGCMC 3 cut(s) 672, 775, 909
BanI GGYRCC 2 cut(s) 770, 904
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 2 cut(s) 672, 862
BbvI GCAGC 6 cut(s) 71, 83, 163, 269, 367, 698
BccI CCATC 4 cut(s) 358, 536, 590, 713
BciT130I CCWGG 3 cut(s) 45, 265, 776
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 2 cut(s) 20, 485
BfmI CTRYAG 3 cut(s) 78, 496, 712
BglII AGATCT 2 cut(s) 286, 565
BisI GCNGC 6 cut(s) 60, 72, 152, 258, 381, 712
BlsI GCNGC 6 cut(s) 61, 73, 153, 259, 382, 713
Bme1390I CCNGG 3 cut(s) 45, 265, 776
BmgT120I GGNCC 1 cut(s) 785
BmiI GGNNCC 2 cut(s) 772, 906
BmrFI CCNGG 3 cut(s) 45, 265, 776
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 2 cut(s) 92, 688
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 465
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 303
BpuEI CTTGAG 2 cut(s) 122, 284
BsaBI GATNNNNATC 1 cut(s) 750
BsaJI CCNNGG 2 cut(s) 43, 774
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 3 cut(s) 286, 344, 712
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 3 cut(s) 45, 265, 776
BseDI CCNNGG 2 cut(s) 43, 774
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 533, 602
BseNI ACTGG 3 cut(s) 286, 344, 712
BseRI GAGGAG 2 cut(s) 106, 125
BseSI GKGCMC 3 cut(s) 672, 775, 909
BseXI GCAGC 6 cut(s) 71, 83, 163, 269, 367, 698
BsgI GTGCAG 1 cut(s) 230
BshFI GGCC 2 cut(s) 16, 786
BshNI GGYRCC 2 cut(s) 770, 904
BsiHKAI GWGCWC 2 cut(s) 672, 862
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 522
BsmBI CGTCTC 1 cut(s) 522
BsnI GGCC 2 cut(s) 16, 786
Bsp1286I GDGCHC 4 cut(s) 672, 775, 862, 909
Bsp143I GATC 4 cut(s) 286, 565, 680, 745
BspANI GGCC 2 cut(s) 16, 786
BspCNI CTCAG 2 cut(s) 532, 601
BspLI GGNNCC 2 cut(s) 772, 906
BspMAI CTGCAG 1 cut(s) 716
BspPI GGATC 2 cut(s) 688, 740
BspT107I GGYRCC 2 cut(s) 770, 904
BsrI ACTGG 3 cut(s) 286, 344, 712
BssECI CCNNGG 2 cut(s) 43, 774
BssMI GATC 4 cut(s) 286, 565, 680, 745
Bst2UI CCWGG 3 cut(s) 45, 265, 776
Bst4CI ACNGT 1 cut(s) 455
Bst6I CTCTTC 1 cut(s) 232
BstAPI GCANNNNNTGC 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 196, 378
BstDEI CTNAG 3 cut(s) 519, 588, 886
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 4 cut(s) 289, 568, 683, 748
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 4 cut(s) 286, 565, 680, 745
BstMWI GCNNNNNNNGC 7 cut(s) 68, 80, 139, 148, 485, 599, 770
BstNI CCWGG 3 cut(s) 45, 265, 776
BstNSI RCATGY 3 cut(s) 146, 380, 467
BstSCI CCNGG 3 cut(s) 43, 263, 774
BstSFI CTRYAG 3 cut(s) 78, 496, 712
BstSLI GKGCMC 3 cut(s) 672, 775, 909
BstV1I GCAGC 6 cut(s) 71, 83, 163, 269, 367, 698
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 3 cut(s) 286, 565, 680
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 3 cut(s) 286, 565, 680
BsuRI GGCC 2 cut(s) 16, 786
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 705
Cac8I GCNNGC 2 cut(s) 196, 378
CaiI CAGNNNCTG 1 cut(s) 707
Cfr13I GGNCC 1 cut(s) 785
CviAII CATG 6 cut(s) 143, 275, 377, 417, 464, 508
DdeI CTNAG 3 cut(s) 519, 588, 886
DpnI GATC 4 cut(s) 288, 567, 682, 747
DpnII GATC 4 cut(s) 286, 565, 680, 745
DraI TTTAAA 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
Eco147I AGGCCT 1 cut(s) 16
Eco32I GATATC 1 cut(s) 814
Eco57I CTGAAG 2 cut(s) 213, 459
EcoRII CCWGG 3 cut(s) 43, 263, 774
EcoRV GATATC 1 cut(s) 814
Esp3I CGTCTC 1 cut(s) 522
FaeI CATG 6 cut(s) 146, 278, 380, 420, 467, 511
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FatI CATG 6 cut(s) 142, 274, 376, 416, 463, 507
FblI GTMKAC 1 cut(s) 457
Fnu4HI GCNGC 6 cut(s) 60, 72, 152, 258, 381, 712
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 6 cut(s) 60, 72, 152, 258, 381, 712
FspBI CTAG 2 cut(s) 20, 485
GluI GCNGC 6 cut(s) 60, 72, 152, 258, 381, 712
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 2 cut(s) 16, 786
Hin1II CATG 6 cut(s) 146, 278, 380, 420, 467, 511
HinfI GANTC 2 cut(s) 113, 751
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 3 cut(s) 458, 670, 839
Hpy188I TCNGA 5 cut(s) 432, 522, 618, 750, 795
Hpy188III TCNNGA 4 cut(s) 103, 231, 290, 817
Hpy8I GTNNAC 3 cut(s) 458, 670, 839
HpyAV CCTTC 1 cut(s) 6
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 7 cut(s) 211, 239, 278, 383, 670, 701, 714
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 80, 139, 148, 485, 599, 770
HpyF3I CTNAG 3 cut(s) 519, 588, 886
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 6 cut(s) 146, 278, 380, 420, 467, 511
Kzo9I GATC 4 cut(s) 286, 565, 680, 745
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 6 cut(s) 71, 83, 163, 269, 367, 698
LweI GCATC 2 cut(s) 92, 688
MaeI CTAG 2 cut(s) 20, 485
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 3 cut(s) 307, 511, 650
MalI GATC 4 cut(s) 288, 567, 682, 747
MboI GATC 4 cut(s) 286, 565, 680, 745
MboII GAAGA 8 cut(s) 219, 455, 465, 560, 575, 634, 675, 891
MflI RGATCY 3 cut(s) 286, 565, 680
MhlI GDGCHC 4 cut(s) 672, 775, 862, 909
MluCI AATT 6 cut(s) 108, 160, 394, 549, 687, 823
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 6 cut(s) 27, 127, 146, 173, 308, 669
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 3 cut(s) 159, 405, 726
MspR9I CCNGG 3 cut(s) 45, 265, 776
MvaI CCWGG 3 cut(s) 45, 265, 776
MwoI GCNNNNNNNGC 7 cut(s) 68, 80, 139, 148, 485, 599, 770
NdeII GATC 4 cut(s) 286, 565, 680, 745
NlaIII CATG 6 cut(s) 146, 278, 380, 420, 467, 511
NlaIV GGNNCC 2 cut(s) 772, 906
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 3 cut(s) 146, 380, 467
PaeI GCATGC 1 cut(s) 380
PceI AGGCCT 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 1 cut(s) 751
PkrI GCNGC 6 cut(s) 61, 73, 153, 259, 382, 713
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PsiI TTATAA 1 cut(s) 919
Psp6I CCWGG 3 cut(s) 43, 263, 774
PspGI CCWGG 3 cut(s) 43, 263, 774
PspN4I GGNNCC 2 cut(s) 772, 906
PspPI GGNCC 1 cut(s) 785
PsrI GAACNNNNNNTAC 2 cut(s) 384, 416
PstI CTGCAG 1 cut(s) 716
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 3 cut(s) 286, 565, 680
SaqAI TTAA 3 cut(s) 159, 405, 726
SatI GCNGC 6 cut(s) 60, 72, 152, 258, 381, 712
Sau3AI GATC 4 cut(s) 286, 565, 680, 745
Sau96I GGNCC 1 cut(s) 785
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 3 cut(s) 45, 265, 776
SduI GDGCHC 4 cut(s) 672, 775, 862, 909
SfaNI GCATC 2 cut(s) 92, 688
SfcI CTRYAG 3 cut(s) 78, 496, 712
SmlI CTYRAG 2 cut(s) 137, 299
SmoI CTYRAG 2 cut(s) 137, 299
SphI GCATGC 1 cut(s) 380
Sse9I AATT 6 cut(s) 108, 160, 394, 549, 687, 823
SseBI AGGCCT 1 cut(s) 16
SspMI CTAG 2 cut(s) 20, 485
StuI AGGCCT 1 cut(s) 16
StyD4I CCNGG 3 cut(s) 43, 263, 774
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 1 cut(s) 518
TaqI TCGA 1 cut(s) 147
TaqII GACCGA 1 cut(s) 326
TasI AATT 6 cut(s) 108, 160, 394, 549, 687, 823
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 3 cut(s) 159, 405, 726
Tru9I TTAA 3 cut(s) 159, 405, 726
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 6 cut(s) 59, 71, 151, 257, 380, 711
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 635
TspRI CASTG 1 cut(s) 712
VneI GTGCAC 1 cut(s) 668
XapI RAATTY 2 cut(s) 394, 549
XceI RCATGY 3 cut(s) 146, 380, 467
XmiI GTMKAC 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 20, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.