AT1G75680

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001649
Physical Location & Seq
Reverse (-)
28417052 .. 28419635
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75680.1

Sequence Viewer

Length: 1578 bp
ATGGAGGAGAAATCCAAGTCAAGAGGTTGGTGCGGTTGGTTCATCGCCATTATTGTGCTAGCTTCTGTTATCCTCGCCGTCGTTTACACTGTTAAATTGAGAACGAAGAAATCCGGTGACGATGACGGTGGCGGTCCCGTTCCTGGACCTCCCGGCGCCATTGATAAGAAATACGCCGACGCTCTTAAGCTCGCTTTGCAGTTCTTCGATATCCAGAAATCTGGTAAATTGGAGAACAATAAGATACCTTGGAGAGGAGATTCAGGTCTTAAAGATGGAAGTGAAGATAATCTGGATCTTTCCAAAGGCTTATATGATGCTGGAGATCATATAAAGTTTGGTTTTCCAATGGCTTTCACTGCTACAGTTTTGTCATGGTCGATTCTTGAGTATGGTGATCAAATGAATGCAGTGAACCAATTGGATCCTGCTAAAGACTCTCTCCGGTGGATCACTGACTATCTTATCAAAGCTCATCCTTCTGACAATGTCCTCTATATCCAGGTGGGAGATCCAAAAGTAGATCATCCATGCTGGGAGAGACCAGAGGATATGAAAGAGAAGAGACCACTTACTAAAATTGATGTAGATACTCCAGGGACAGAGGTTGCTGCTGAAACTGCTGCAGCTATGGCTTCAGCGTCTTTGGTGTTTAAGGATAGTGATCCTACATATTCAGCAACGCTTCTGAAACATGCGAAGCAGTTGTTTAATTTTGCAGATACAAAGAGAGGCTCTTACAGTGTTAACATACCTGAGGTTCAGAAGTTTTACAATTCGACTGGATATGGTGATGAGCTACTATGGGCAGCTAGTTGGTTGTATCATGCAACAGAGGATAAAACTTACCTTGATTATGTGTCTAATCATGGAAAAGAATTTGCTAGTTTTGGAAATCCTACTTGGTTTAGTTGGGACAACAAGCTTGCAGGAACACAGGTACTATTATCAAGATTACTCTTCTTTAAGAAAGATTTATCAGGAAGCAAGGGACTTGGAAATTACAGGAACACAGCTAAAGCTGTCATGTGTGGACTTCTACCAAAGTCTCCAACATCTACAGCTAGTAGAACAAACGGTGGTCTTATATGGGTTAGTGAATGGAACTCGATGCAACAATCCGTTTCGTCAGCGTTTTTAGCCTCGCTTTTCAGTGATTACATGCTCACTTCCCGTATCCATAAAATATCTTGCGACGGGAAAATCTTCAAAGCAACAGAGCTTAGAGATTTCGCCAAATCGCAGGCTGATTACATGCTGGGGAAGAATCCGTTGGGAACGAGCTTCGTGGTGGGTTATGGAGACAAATACCCACAATTTGTGCATCATAGAGGAGCTTCGATCCCGGCAGATGCAACAACGGGTTGCTTAGATGGATTCAAATGGTTTAACTCGACGAAACCAAACCCAAACATAGCATATGGTGCACTCGTAGGTGGACCTTTCTTCAATGAGACGTTCACTGACTCACGAGAGAACCCAATGCAGAACGAGCCAACCACTTACAACAATGCACTCCTCGTTGGTCTCTTGTCTAGTCTTGTCACTACATCTTCTACTTTACAGTCGTTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

525

Amino Acids

57.87

Weight (kDa)

6.77

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 58 - 511 7e-164 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AciI CCGC 2 cut(s) 33, 132
AclWI GGATC 7 cut(s) 303, 419, 432, 458, 506, 659, 1336
AcsI RAATTY 1 cut(s) 878
AcuI CTGAAG 1 cut(s) 621
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 1 cut(s) 942
AfiI CCNNNNNNNGG 2 cut(s) 143, 254
AflII CTTAAG 1 cut(s) 185
AgsI TTSAA 4 cut(s) 1210, 1381, 1450, 1573
AjnI CCWGG 3 cut(s) 142, 501, 595
AjuI GAANNNNNNNTTGG 4 cut(s) 886, 918, 1256, 1288
Alw21I GWGCWC 1 cut(s) 1429
Alw26I GTCTC 6 cut(s) 535, 559, 1053, 1296, 1448, 1532
Alw44I GTGCAC 1 cut(s) 1425
AlwI GGATC 7 cut(s) 303, 419, 432, 458, 506, 659, 1336
ApaLI GTGCAC 1 cut(s) 1425
ApeKI GCWGC 4 cut(s) 611, 623, 626, 809
ApoI RAATTY 1 cut(s) 878
Asp700I GAANNNNTTC 1 cut(s) 1205
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 134, 146, 1439
AsuC2I CCSGG 2 cut(s) 153, 1346
AsuHPI GGTGA 3 cut(s) 128, 407, 803
AsuNHI GCTAGC 1 cut(s) 58
AvaII GGWCC 3 cut(s) 134, 146, 1439
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 1429
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 1470
Bbv12I GWGCWC 1 cut(s) 1429
BbvI GCAGC 4 cut(s) 598, 610, 638, 821
BccI CCATC 2 cut(s) 269, 1367
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 3 cut(s) 144, 503, 597
BciVI GTATCC 1 cut(s) 1187
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 2 cut(s) 153, 1346
BcoDI GTCTC 6 cut(s) 535, 559, 1053, 1296, 1448, 1532
BfaI CTAG 5 cut(s) 59, 813, 885, 1065, 1536
BfmI CTRYAG 3 cut(s) 363, 624, 1059
BfoI RGCGCY 1 cut(s) 159
BfrI CTTAAG 1 cut(s) 185
BfuI GTATCC 1 cut(s) 1187
BisI GCNGC 4 cut(s) 612, 624, 627, 810
BlsI GCNGC 4 cut(s) 613, 625, 628, 811
Bme1390I CCNGG 5 cut(s) 144, 153, 503, 597, 1346
Bme18I GGWCC 3 cut(s) 134, 146, 1439
BmgT120I GGNCC 3 cut(s) 134, 146, 1439
BmiI GGNNCC 3 cut(s) 136, 157, 426
BmrFI CCNGG 5 cut(s) 144, 153, 503, 597, 1346
BmsI GCATC 4 cut(s) 307, 1101, 1333, 1342
BmtI GCTAGC 1 cut(s) 62
BpmI CTGGAG 2 cut(s) 342, 579
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 2 cut(s) 153, 1346
BsaBI GATNNNNATC 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 3 cut(s) 535, 559, 1532
BsaJI CCNNGG 2 cut(s) 248, 596
BsaWI WCCGGW 2 cut(s) 113, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 254
Bse1I ACTGG 1 cut(s) 787
Bse21I CCTNAGG 1 cut(s) 756
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 3 cut(s) 144, 503, 597
BseDI CCNNGG 2 cut(s) 248, 596
BseGI GGATG 2 cut(s) 475, 526
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 2 cut(s) 143, 254
BseMII CTCAG 1 cut(s) 747
BseNI ACTGG 1 cut(s) 787
BseRI GAGGAG 4 cut(s) 20, 270, 1347, 1508
BseSI GKGCMC 1 cut(s) 1429
BseXI GCAGC 4 cut(s) 598, 610, 638, 821
BseYI CCCAGC 2 cut(s) 534, 1258
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 1429
BsiSI CCGG 4 cut(s) 114, 153, 445, 1346
BslFI GGGAC 4 cut(s) 120, 613, 929, 1005
BslI CCNNNNNNNGG 2 cut(s) 143, 254
BsmAI GTCTC 6 cut(s) 535, 559, 1053, 1296, 1448, 1532
BsmBI CGTCTC 1 cut(s) 1448
BsmFI GGGAC 4 cut(s) 120, 613, 929, 1005
BsmI GAATGC 1 cut(s) 412
Bso31I GGTCTC 3 cut(s) 535, 559, 1532
Bsp1286I GDGCHC 1 cut(s) 1429
Bsp143I GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
BspACI CCGC 2 cut(s) 33, 132
BspCNI CTCAG 1 cut(s) 748
BspLI GGNNCC 3 cut(s) 136, 157, 426
BspMAI CTGCAG 1 cut(s) 628
BspOI GCTAGC 1 cut(s) 62
BspPI GGATC 7 cut(s) 303, 419, 432, 458, 506, 659, 1336
BspT107I GGYRCC 1 cut(s) 155
BspTI CTTAAG 1 cut(s) 185
BspTNI GGTCTC 3 cut(s) 535, 559, 1532
BsrI ACTGG 1 cut(s) 787
BssECI CCNNGG 2 cut(s) 248, 596
BssMI GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
BssNI GRCGYC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 1470
BssT1I CCWWGG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 1470
Bst2UI CCWGG 3 cut(s) 144, 503, 597
Bst4CI ACNGT 6 cut(s) 91, 128, 367, 743, 1079, 1566
Bst6I CTCTTC 2 cut(s) 557, 965
BstACI GRCGYC 1 cut(s) 156
BstAFI CTTAAG 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 1424
BstC8I GCNNGC 4 cut(s) 60, 192, 927, 1245
BstDEI CTNAG 3 cut(s) 756, 1223, 1369
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 475, 526
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 9 cut(s) 298, 328, 400, 427, 453, 514, 526, 667, 1344
BstMAI GTCTC 6 cut(s) 535, 559, 1053, 1296, 1448, 1532
BstMBI GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
BstMWI GCNNNNNNNGC 7 cut(s) 196, 359, 620, 632, 1139, 1424, 1492
BstNI CCWGG 3 cut(s) 144, 503, 597
BstNSI RCATGY 3 cut(s) 698, 1165, 1258
BstSCI CCNGG 5 cut(s) 142, 151, 501, 595, 1344
BstSFI CTRYAG 3 cut(s) 363, 624, 1059
BstSLI GKGCMC 1 cut(s) 1429
BstV1I GCAGC 4 cut(s) 598, 610, 638, 821
BstX2I RGATCY 3 cut(s) 295, 424, 511
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 3 cut(s) 295, 424, 511
Bsu36I CCTNAGG 1 cut(s) 756
BsuI GTATCC 1 cut(s) 1187
BtgZI GCGATG 1 cut(s) 28
BtsCI GGATG 2 cut(s) 475, 526
BtsI GCAGTG 2 cut(s) 357, 417
BtsIMutI CAGTG 7 cut(s) 87, 357, 417, 453, 748, 1159, 1461
Cac8I GCNNGC 4 cut(s) 60, 192, 927, 1245
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 134, 146, 1439
CseI GACGC 2 cut(s) 188, 630
Csp6I GTAC 1 cut(s) 941
CviAII CATG 8 cut(s) 375, 531, 695, 827, 869, 1027, 1162, 1255
CviQI GTAC 1 cut(s) 941
DdeI CTNAG 3 cut(s) 756, 1223, 1369
DinI GGCGCC 1 cut(s) 157
DpnI GATC 9 cut(s) 297, 327, 399, 426, 452, 513, 525, 666, 1343
DpnII GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
Eam1104I CTCTTC 2 cut(s) 557, 965
EarI CTCTTC 2 cut(s) 557, 965
Eco130I CCWWGG 1 cut(s) 248
Eco31I GGTCTC 3 cut(s) 535, 559, 1532
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 3 cut(s) 134, 146, 1439
Eco57I CTGAAG 1 cut(s) 621
Eco81I CCTNAGG 1 cut(s) 756
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoRII CCWGG 3 cut(s) 142, 501, 595
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 248
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 248
Esp3I CGTCTC 1 cut(s) 1448
FaeI CATG 8 cut(s) 378, 534, 698, 830, 872, 1030, 1165, 1258
FaqI GGGAC 4 cut(s) 120, 613, 929, 1005
FatI CATG 8 cut(s) 374, 530, 694, 826, 868, 1026, 1161, 1254
FauNDI CATATG 1 cut(s) 1420
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 4 cut(s) 612, 624, 627, 810
FokI GGATG 2 cut(s) 462, 513
Fsp4HI GCNGC 4 cut(s) 612, 624, 627, 810
FspBI CTAG 5 cut(s) 59, 813, 885, 1065, 1536
GlaI GCGC 1 cut(s) 157
GluI GCNGC 4 cut(s) 612, 624, 627, 810
GsaI CCCAGC 2 cut(s) 538, 1262
GsuI CTGGAG 2 cut(s) 342, 579
HaeII RGCGCY 1 cut(s) 159
HapII CCGG 4 cut(s) 114, 153, 445, 1346
HgaI GACGC 2 cut(s) 188, 630
HhaI GCGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 8 cut(s) 378, 534, 698, 830, 872, 1030, 1165, 1258
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 6 cut(s) 260, 382, 437, 1267, 1377, 1466
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 4 cut(s) 114, 153, 445, 1346
HphI GGTGA 3 cut(s) 128, 407, 803
Hpy166II GTNNAC 7 cut(s) 85, 415, 748, 1034, 1427, 1439, 1461
Hpy188I TCNGA 3 cut(s) 484, 690, 765
Hpy188III TCNNGA 7 cut(s) 21, 214, 293, 386, 951, 981, 1470
Hpy8I GTNNAC 7 cut(s) 85, 415, 748, 1034, 1427, 1439, 1461
Hpy99I CGWCG 4 cut(s) 83, 182, 1199, 1399
HpyAV CCTTC 1 cut(s) 489
HpyCH4III ACNGT 6 cut(s) 91, 128, 367, 743, 1079, 1566
HpyCH4IV ACGT 1 cut(s) 1457
HpyF10VI GCNNNNNNNGC 7 cut(s) 196, 359, 620, 632, 1139, 1424, 1492
HpyF3I CTNAG 3 cut(s) 756, 1223, 1369
HpySE526I ACGT 1 cut(s) 1457
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 8 cut(s) 378, 534, 698, 830, 872, 1030, 1165, 1258
HspAI GCGC 1 cut(s) 156
KasI GGCGCC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 397
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
LmnI GCTCC 1 cut(s) 1334
Lsp1109I GCAGC 4 cut(s) 598, 610, 638, 821
LweI GCATC 4 cut(s) 307, 1101, 1333, 1342
MaeI CTAG 5 cut(s) 59, 813, 885, 1065, 1536
MaeII ACGT 1 cut(s) 1457
MaeIII GTNAC 2 cut(s) 116, 1543
MalI GATC 9 cut(s) 297, 327, 399, 426, 452, 513, 525, 666, 1343
MboI GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
MboII GAAGA 9 cut(s) 118, 196, 296, 574, 952, 1198, 1276, 1438, 1545
MfeI CAATTG 1 cut(s) 419
MflI RGATCY 3 cut(s) 295, 424, 511
MhlI GDGCHC 1 cut(s) 1429
MluCI AATT 9 cut(s) 95, 227, 419, 579, 712, 775, 878, 1000, 1316
Mly113I GGCGCC 1 cut(s) 156
MlyI GAGTC 2 cut(s) 431, 1460
MmeI TCCRAC 1 cut(s) 1076
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 8 cut(s) 93, 186, 270, 654, 711, 747, 966, 1389
MslI CAYNNNNRTG 1 cut(s) 53
MspCI CTTAAG 1 cut(s) 185
MspI CCGG 4 cut(s) 114, 153, 445, 1346
MspR9I CCNGG 5 cut(s) 144, 153, 503, 597, 1346
MunI CAATTG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 3 cut(s) 144, 503, 597
MwoI GCNNNNNNNGC 7 cut(s) 196, 359, 620, 632, 1139, 1424, 1492
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 2 cut(s) 153, 1346
NdeI CATATG 1 cut(s) 1420
NdeII GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
NheI GCTAGC 1 cut(s) 58
NlaIII CATG 8 cut(s) 378, 534, 698, 830, 872, 1030, 1165, 1258
NlaIV GGNNCC 3 cut(s) 136, 157, 426
NmuCI GTSAC 2 cut(s) 116, 1543
NspI RCATGY 3 cut(s) 698, 1165, 1258
PctI GAATGC 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 4 cut(s) 260, 382, 1267, 1377
PflFI GACNNNGTC 1 cut(s) 488
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 4 cut(s) 613, 625, 628, 811
PleI GAGTC 2 cut(s) 431, 1460
PluTI GGCGCC 1 cut(s) 159
PpsI GAGTC 2 cut(s) 431, 1460
Psp6I CCWGG 3 cut(s) 142, 501, 595
PspFI CCCAGC 2 cut(s) 534, 1258
PspGI CCWGG 3 cut(s) 142, 501, 595
PspN4I GGNNCC 3 cut(s) 136, 157, 426
PspPI GGNCC 3 cut(s) 134, 146, 1439
PstI CTGCAG 1 cut(s) 628
PsuI RGATCY 3 cut(s) 295, 424, 511
PsyI GACNNNGTC 1 cut(s) 488
RsaI GTAC 1 cut(s) 942
RsaNI GTAC 1 cut(s) 941
RseI CAYNNNNRTG 1 cut(s) 53
SaqAI TTAA 8 cut(s) 93, 186, 270, 654, 711, 747, 966, 1389
SatI GCNGC 4 cut(s) 612, 624, 627, 810
Sau3AI GATC 9 cut(s) 295, 325, 397, 424, 450, 511, 523, 664, 1341
Sau96I GGNCC 3 cut(s) 134, 146, 1439
SchI GAGTC 2 cut(s) 431, 1460
ScrFI CCNGG 5 cut(s) 144, 153, 503, 597, 1346
SduI GDGCHC 1 cut(s) 1429
SfaNI GCATC 4 cut(s) 307, 1101, 1333, 1342
SfcI CTRYAG 3 cut(s) 363, 624, 1059
SfoI GGCGCC 1 cut(s) 157
SinI GGWCC 3 cut(s) 134, 146, 1439
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 2 cut(s) 185, 386
SmoI CTYRAG 2 cut(s) 185, 386
Sse9I AATT 9 cut(s) 95, 227, 419, 579, 712, 775, 878, 1000, 1316
SsiI CCGC 2 cut(s) 33, 132
SspDI GGCGCC 1 cut(s) 155
SspMI CTAG 5 cut(s) 59, 813, 885, 1065, 1536
StyD4I CCNGG 5 cut(s) 142, 151, 501, 595, 1344
StyI CCWWGG 1 cut(s) 248
TaaI ACNGT 6 cut(s) 91, 128, 367, 743, 1079, 1566
TaiI ACGT 1 cut(s) 1460
TaqI TCGA 6 cut(s) 207, 380, 779, 1109, 1340, 1394
TasI AATT 9 cut(s) 95, 227, 419, 579, 712, 775, 878, 1000, 1316
TfiI GAWTC 4 cut(s) 260, 382, 1267, 1377
Tru1I TTAA 8 cut(s) 93, 186, 270, 654, 711, 747, 966, 1389
Tru9I TTAA 8 cut(s) 93, 186, 270, 654, 711, 747, 966, 1389
TscAI CASTG 7 cut(s) 94, 364, 417, 460, 748, 1159, 1468
TseFI GTSAC 2 cut(s) 116, 1543
TseI GCWGC 4 cut(s) 611, 623, 626, 809
Tsp45I GTSAC 2 cut(s) 116, 1543
TspDTI ATGAA 3 cut(s) 31, 419, 569
TspGWI ACGGA 2 cut(s) 1111, 1260
TspRI CASTG 7 cut(s) 94, 364, 417, 460, 748, 1159, 1468
Tth111I GACNNNGTC 1 cut(s) 488
Vha464I CTTAAG 1 cut(s) 185
VneI GTGCAC 1 cut(s) 1425
VpaK11BI GGWCC 3 cut(s) 134, 146, 1439
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 1 cut(s) 878
XceI RCATGY 3 cut(s) 698, 1165, 1258
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 5 cut(s) 59, 813, 885, 1065, 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.