Rh2AG130500

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001649
Physical Location & Seq
Forward (+)
11803322 .. 11804452
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG130500.1

Sequence Viewer

Length: 789 bp
ATGACAGAGAGAAGGCCTCTAGTTCAAATCAACACATCTTTCCCAGGAACAGAAGTGGCAGCAGAAACAGCAGCAGCTATGGCATCAGCATATCTTGTTTTCAGGAAAATCGACTCCTCATACTCAAAATCGCTCCTCAAGCATGCCGAGCAGCTGTTTAATTTTGCCGATACTTATAGAGGTTCTTATAGTGCCAGCATACCCCAAGTGCATGGGTTCTACAACTCTTCAGGATATGGAGATGAGCTATTATGGGCAACTACCTGGCTCTACCATGCAACTGGAGATCTGGACTACCTCAAGTTTGTGACCGAACATAATGGGCCAACATTTGCTAACTGGGGAAGTCCATCTTGGTTTAGTTGGGCTGAAAAGCATGCTGTAACTCAAGTTTTTATGTCCCGGATAAGTTTCTTCGGTGCAAAAGAGCTCCCTATGGCAGAGAACCTTGATCTCCAGATGTACCGAAAAACTGCAGAGGTCTTCTTGTGTGGACTTATACCTGATTCGCCTACGGCCGCACCTGACAGAACAGATGGTCTGATATGGGTGGAAGAATGGAACTGTCTACAACATGCTATGTCTTCAGCCTTTCTAGCAGTTCTCTATGGTGACTACATGGTTACATCTCAGACTGGAATGTTGTATGGTGATGGGAAAATTTACAAGCCTGAAGATCTTCGTAGCTTCTCCATCTCTCAGAACCACATTGCACAAATACTTGCAGGCAGATTACGCGTTGGGAAAAAATTCGATGAAGATGAGTTGTCTTGTAGGGTATGGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

262

Amino Acids

29.52

Weight (kDa)

5.49

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 234 6.2e-56 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 568
AccII CGCG 1 cut(s) 738
AciI CCGC 1 cut(s) 519
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 2 cut(s) 660, 749
AcuI CTGAAG 3 cut(s) 213, 570, 693
AfaI GTAC 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 736
AgsI TTSAA 1 cut(s) 26
AjnI CCWGG 2 cut(s) 43, 263
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 5 cut(s) 77, 154, 247, 430, 687
AluI AGCT 5 cut(s) 77, 154, 247, 430, 687
Alw21I GWGCWC 1 cut(s) 432
AoxI GGCC 3 cut(s) 14, 323, 516
ApeKI GCWGC 4 cut(s) 59, 71, 74, 151
ApoI RAATTY 2 cut(s) 660, 749
Asp700I GAANNNNTTC 2 cut(s) 678, 749
AspS9I GGNCC 1 cut(s) 323
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 623, 662
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 2 cut(s) 475, 576
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 4 cut(s) 71, 83, 86, 163
BccI CCATC 4 cut(s) 358, 530, 647, 701
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 2 cut(s) 45, 265
BcnI CCSGG 1 cut(s) 403
BfaI CTAG 2 cut(s) 20, 596
BfmI CTRYAG 1 cut(s) 474
BglII AGATCT 2 cut(s) 286, 676
BisI GCNGC 5 cut(s) 60, 72, 75, 152, 519
BlsI GCNGC 5 cut(s) 61, 73, 76, 153, 520
Bme1390I CCNGG 3 cut(s) 45, 265, 403
BmgT120I GGNCC 1 cut(s) 323
BmrFI CCNGG 3 cut(s) 45, 265, 403
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 2 cut(s) 475, 576
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 303, 440
BpuEI CTTGAG 3 cut(s) 122, 284, 372
BpuMI CCSGG 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 43
Bse1I ACTGG 3 cut(s) 286, 344, 640
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 2 cut(s) 45, 265
BseDI CCNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 2 cut(s) 644, 713
BseNI ACTGG 3 cut(s) 286, 344, 640
BseRI GAGGAG 2 cut(s) 106, 125
BseX3I CGGCCG 1 cut(s) 516
BseXI GCAGC 4 cut(s) 71, 83, 86, 163
Bsh1236I CGCG 1 cut(s) 738
Bsh1285I CGRYCG 1 cut(s) 519
BshFI GGCC 3 cut(s) 16, 325, 518
BsiEI CGRYCG 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 3 cut(s) 16, 325, 518
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 3 cut(s) 286, 451, 676
BspACI CCGC 1 cut(s) 519
BspANI GGCC 3 cut(s) 16, 325, 518
BspCNI CTCAG 2 cut(s) 643, 712
BspFNI CGCG 1 cut(s) 738
BspMAI CTGCAG 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 708
BsrI ACTGG 3 cut(s) 286, 344, 640
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 3 cut(s) 286, 451, 676
Bst2UI CCWGG 2 cut(s) 45, 265
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 1 cut(s) 232
BstC8I GCNNGC 4 cut(s) 144, 196, 378, 727
BstDEI CTNAG 2 cut(s) 630, 699
BstFNI CGCG 1 cut(s) 738
BstKTI GATC 3 cut(s) 289, 454, 679
BstMBI GATC 3 cut(s) 286, 451, 676
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 6 cut(s) 68, 80, 139, 148, 596, 735
BstNI CCWGG 2 cut(s) 45, 265
BstNSI RCATGY 3 cut(s) 146, 380, 578
BstSCI CCNGG 3 cut(s) 43, 263, 401
BstSFI CTRYAG 1 cut(s) 474
BstUI CGCG 1 cut(s) 738
BstV1I GCAGC 4 cut(s) 71, 83, 86, 163
BstV2I GAAGAC 2 cut(s) 475, 576
BstX2I RGATCY 2 cut(s) 286, 676
BstXI CCANNNNNNTGG 2 cut(s) 212, 281
BstYI RGATCY 2 cut(s) 286, 676
BstZI CGGCCG 1 cut(s) 516
BsuRI GGCC 3 cut(s) 16, 325, 518
Cac8I GCNNGC 4 cut(s) 144, 196, 378, 727
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 463
CviAII CATG 6 cut(s) 143, 212, 275, 377, 575, 619
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 2 cut(s) 630, 699
DpnI GATC 3 cut(s) 288, 453, 678
DpnII GATC 3 cut(s) 286, 451, 676
EaeI YGGCCR 1 cut(s) 516
EagI CGGCCG 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 432
Eco52I CGGCCG 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 3 cut(s) 213, 570, 693
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 43, 263
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 6 cut(s) 146, 215, 278, 380, 578, 622
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FaqI GGGAC 1 cut(s) 385
FatI CATG 6 cut(s) 142, 211, 274, 376, 574, 618
FblI GTMKAC 1 cut(s) 568
Fnu4HI GCNGC 5 cut(s) 60, 72, 75, 152, 519
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 5 cut(s) 60, 72, 75, 152, 519
FspBI CTAG 2 cut(s) 20, 596
GluI GCNGC 5 cut(s) 60, 72, 75, 152, 519
GsuI CTGGAG 2 cut(s) 303, 440
HaeIII GGCC 3 cut(s) 16, 325, 518
HapII CCGG 1 cut(s) 403
Hin1II CATG 6 cut(s) 146, 215, 278, 380, 578, 622
HinfI GANTC 2 cut(s) 113, 506
HpaII CCGG 1 cut(s) 403
HphI GGTGA 2 cut(s) 623, 662
Hpy166II GTNNAC 2 cut(s) 494, 569
Hpy188I TCNGA 3 cut(s) 543, 633, 702
Hpy188III TCNNGA 4 cut(s) 103, 231, 290, 457
Hpy8I GTNNAC 2 cut(s) 494, 569
HpyAV CCTTC 1 cut(s) 6
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4V TGCA 6 cut(s) 211, 278, 422, 476, 713, 725
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 80, 139, 148, 596, 735
HpyF3I CTNAG 2 cut(s) 630, 699
Hsp92II CATG 6 cut(s) 146, 215, 278, 380, 578, 622
Kzo9I GATC 3 cut(s) 286, 451, 676
LmnI GCTCC 2 cut(s) 138, 435
Lsp1109I GCAGC 4 cut(s) 71, 83, 86, 163
LweI GCATC 1 cut(s) 92
MaeI CTAG 2 cut(s) 20, 596
MaeIII GTNAC 4 cut(s) 307, 382, 611, 622
MalI GATC 3 cut(s) 288, 453, 678
MboI GATC 3 cut(s) 286, 451, 676
MboII GAAGA 8 cut(s) 219, 406, 475, 566, 576, 671, 686, 770
MflI RGATCY 2 cut(s) 286, 676
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 3 cut(s) 160, 660, 749
MluI ACGCGT 1 cut(s) 736
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 6 cut(s) 27, 127, 146, 173, 308, 472
MroXI GAANNNNTTC 2 cut(s) 678, 749
MseI TTAA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 3 cut(s) 45, 265, 403
MvaI CCWGG 2 cut(s) 45, 265
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 6 cut(s) 68, 80, 139, 148, 596, 735
NciI CCSGG 1 cut(s) 403
NdeII GATC 3 cut(s) 286, 451, 676
NlaIII CATG 6 cut(s) 146, 215, 278, 380, 578, 622
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 2 cut(s) 307, 611
NspI RCATGY 3 cut(s) 146, 380, 578
PaeI GCATGC 2 cut(s) 146, 380
PceI AGGCCT 1 cut(s) 16
PdmI GAANNNNTTC 2 cut(s) 678, 749
PfeI GAWTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 401
PkrI GCNGC 5 cut(s) 61, 73, 76, 153, 520
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 43, 263
PspGI CCWGG 2 cut(s) 43, 263
PspPI GGNCC 1 cut(s) 323
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 2 cut(s) 286, 676
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 5 cut(s) 60, 72, 75, 152, 519
Sau3AI GATC 3 cut(s) 286, 451, 676
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 3 cut(s) 45, 265, 403
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 474
SmlI CTYRAG 3 cut(s) 137, 299, 387
SmoI CTYRAG 3 cut(s) 137, 299, 387
SphI GCATGC 2 cut(s) 146, 380
Sse9I AATT 3 cut(s) 160, 660, 749
SseBI AGGCCT 1 cut(s) 16
SsiI CCGC 1 cut(s) 519
SspMI CTAG 2 cut(s) 20, 596
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 1 cut(s) 16
StyD4I CCNGG 3 cut(s) 43, 263, 401
TaaI ACNGT 1 cut(s) 566
TaqI TCGA 2 cut(s) 111, 753
TaqII GACCGA 1 cut(s) 326
TasI AATT 3 cut(s) 160, 660, 749
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 2 cut(s) 307, 611
TseI GCWGC 4 cut(s) 59, 71, 74, 151
Tsp45I GTSAC 2 cut(s) 307, 611
TspDTI ATGAA 1 cut(s) 771
XapI RAATTY 2 cut(s) 660, 749
XceI RCATGY 3 cut(s) 146, 380, 578
XmiI GTMKAC 1 cut(s) 568
XmnI GAANNNNTTC 2 cut(s) 678, 749
XspI CTAG 2 cut(s) 20, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.