Rh2BG133600

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001649
Physical Location & Seq
Forward (+)
11403422 .. 11404820
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG133600.1

Sequence Viewer

Length: 1032 bp
ATGACAGAGAGAAGGCCTCTAGTTCAAATCAACACATCTTTCCCAGGAACAGAAGTGGCAGCAGAAACAGCAGCAGCTATGGCATCAGCATATCTTGTTTTCAGGAAAATCGACTCCTCATACTCAAAATCGCTCCTCAAGCATGCCGAGCAGCTGTTTAATTTTGCCGATACTTATAGAGGTTCTTATAGTGCCAGCATACCCCAAGTGCATGGGTTCTACAACTCTTCAGGATATGGAGATGAGCTATTATGGGCAACTACCTGGCTCTACCATGCAACTGGAGATCTGGACTACCTCAAGTTTGTGACCGAACATAATGGGCCAACATTTGCTAACTGGGGAAGTCCATCTTGGTTTAGTTGGGCTGAAAAGCATGCTGTAACTCAAGTTTTTATGTCCCGGATAAGTTTCTTCGGTGCAAAAGAGCTCCCTATGGCAGAGAACCTTGATCTCCAGATGTACCGAAAAACTGCAGAGGTCTTCTTGTGTGGACTTATACCTGATTCGCCTACGGCCGCACCTGACAGAACAGATGGTCTGATATGGGTGGAAGAATGGAACTGTCTACAACATGCTATGTCTTCAGCCTTTCTAGCAGTTCTCTATGGTGACTACATGGTTACATCTCAGACTGGAATGTTGTATGGTGATGGGAAAATTTACAAGCCTGAAGATCTTCGTAGCTTCTCCATCTCTCAGGCAGATTACGCGTTGGGAAAAAATTCGATGAAGATGAGTTGTCTTGTAGGGTATGGGGGTAACTACCCTCAACAAGTGCACCATAGGGGATCTTCAATTCCGGTTGATGCAGACACTGGCTGCAGAGATGGGTTTAAATGGCTGTACTCCCCCGATCCGAATCCAAATATAGTTGTTGGTGCCCTGGTTGGTGGCCCTTTTCAGAATGATAGCTATATGGATATCAGGAACAATTCAAAGCAAGGTGAACCAAGCACTTACAATAGTGCTCTCATTGTTGCCCTGCTCTCTGGCTTAGTTTCCACTTCTTTGGTGCCCAAGCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

343

Amino Acids

37.98

Weight (kDa)

5.57

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 330 1.4e-91 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1030
AccB1I GGYRCC 2 cut(s) 881, 1015
AccI GTMKAC 1 cut(s) 568
AccII CGCG 1 cut(s) 713
AciI CCGC 1 cut(s) 519
AclWI GGATC 2 cut(s) 799, 851
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 2 cut(s) 660, 724
AcuI CTGAAG 3 cut(s) 213, 570, 693
AfaI GTAC 2 cut(s) 464, 848
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 3 cut(s) 26, 798, 939
AjnI CCWGG 3 cut(s) 43, 263, 885
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 6 cut(s) 77, 154, 247, 430, 687, 915
AluI AGCT 6 cut(s) 77, 154, 247, 430, 687, 915
Alw21I GWGCWC 3 cut(s) 432, 783, 973
Alw44I GTGCAC 1 cut(s) 779
AlwI GGATC 2 cut(s) 799, 851
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 4 cut(s) 14, 323, 516, 895
ApaLI GTGCAC 1 cut(s) 779
ApeKI GCWGC 5 cut(s) 59, 71, 74, 151, 822
ApoI RAATTY 2 cut(s) 660, 724
Asp700I GAANNNNTTC 2 cut(s) 678, 724
AspS9I GGNCC 2 cut(s) 323, 896
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 3 cut(s) 623, 662, 959
BaeGI GKGCMC 3 cut(s) 783, 886, 1020
BanI GGYRCC 2 cut(s) 881, 1015
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 2 cut(s) 475, 576
Bbv12I GWGCWC 3 cut(s) 432, 783, 973
BbvI GCAGC 5 cut(s) 71, 83, 86, 163, 809
BccI CCATC 5 cut(s) 358, 530, 647, 701, 824
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 3 cut(s) 45, 265, 887
BcnI CCSGG 1 cut(s) 403
BfaI CTAG 2 cut(s) 20, 596
BfmI CTRYAG 2 cut(s) 474, 823
BglII AGATCT 2 cut(s) 286, 676
BisI GCNGC 6 cut(s) 60, 72, 75, 152, 519, 823
BlsI GCNGC 6 cut(s) 61, 73, 76, 153, 520, 824
Bme1390I CCNGG 4 cut(s) 45, 265, 403, 887
BmgT120I GGNCC 2 cut(s) 323, 896
BmiI GGNNCC 2 cut(s) 883, 1017
BmrFI CCNGG 4 cut(s) 45, 265, 403, 887
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 2 cut(s) 92, 799
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 2 cut(s) 475, 576
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 303, 440
BpuEI CTTGAG 3 cut(s) 122, 284, 372
BpuMI CCSGG 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 861
BsaJI CCNNGG 2 cut(s) 43, 885
BsaWI WCCGGW 1 cut(s) 802
Bse1I ACTGG 4 cut(s) 286, 344, 640, 823
Bse8I GATNNNNATC 1 cut(s) 861
BseBI CCWGG 3 cut(s) 45, 265, 887
BseDI CCNNGG 2 cut(s) 43, 885
BseJI GATNNNNATC 1 cut(s) 861
BseMII CTCAG 2 cut(s) 644, 713
BseNI ACTGG 4 cut(s) 286, 344, 640, 823
BseRI GAGGAG 2 cut(s) 106, 125
BseSI GKGCMC 3 cut(s) 783, 886, 1020
BseX3I CGGCCG 1 cut(s) 516
BseXI GCAGC 5 cut(s) 71, 83, 86, 163, 809
Bsh1236I CGCG 1 cut(s) 713
Bsh1285I CGRYCG 1 cut(s) 519
BshFI GGCC 4 cut(s) 16, 325, 518, 897
BshNI GGYRCC 2 cut(s) 881, 1015
BsiEI CGRYCG 1 cut(s) 519
BsiHKAI GWGCWC 3 cut(s) 432, 783, 973
BsiSI CCGG 2 cut(s) 403, 803
BslFI GGGAC 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 4 cut(s) 16, 325, 518, 897
Bsp1286I GDGCHC 5 cut(s) 432, 783, 886, 973, 1020
Bsp143I GATC 5 cut(s) 286, 451, 676, 791, 856
BspACI CCGC 1 cut(s) 519
BspANI GGCC 4 cut(s) 16, 325, 518, 897
BspCNI CTCAG 2 cut(s) 643, 712
BspFNI CGCG 1 cut(s) 713
BspLI GGNNCC 2 cut(s) 883, 1017
BspMAI CTGCAG 2 cut(s) 478, 827
BspPI GGATC 2 cut(s) 799, 851
BspT107I GGYRCC 2 cut(s) 881, 1015
BsrI ACTGG 4 cut(s) 286, 344, 640, 823
BssECI CCNNGG 2 cut(s) 43, 885
BssMI GATC 5 cut(s) 286, 451, 676, 791, 856
Bst2UI CCWGG 3 cut(s) 45, 265, 887
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 1 cut(s) 232
BstC8I GCNNGC 3 cut(s) 144, 196, 378
BstDEI CTNAG 3 cut(s) 630, 699, 997
BstFNI CGCG 1 cut(s) 713
BstKTI GATC 5 cut(s) 289, 454, 679, 794, 859
BstMBI GATC 5 cut(s) 286, 451, 676, 791, 856
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 6 cut(s) 68, 80, 139, 148, 596, 710
BstNI CCWGG 3 cut(s) 45, 265, 887
BstNSI RCATGY 3 cut(s) 146, 380, 578
BstSCI CCNGG 4 cut(s) 43, 263, 401, 885
BstSFI CTRYAG 2 cut(s) 474, 823
BstSLI GKGCMC 3 cut(s) 783, 886, 1020
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 5 cut(s) 71, 83, 86, 163, 809
BstV2I GAAGAC 2 cut(s) 475, 576
BstX2I RGATCY 3 cut(s) 286, 676, 791
BstXI CCANNNNNNTGG 3 cut(s) 212, 281, 1012
BstYI RGATCY 3 cut(s) 286, 676, 791
BstZI CGGCCG 1 cut(s) 516
BsuRI GGCC 4 cut(s) 16, 325, 518, 897
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 3 cut(s) 144, 196, 378
CaiI CAGNNNCTG 1 cut(s) 818
Cfr13I GGNCC 2 cut(s) 323, 896
Csp6I GTAC 2 cut(s) 463, 847
CviAII CATG 6 cut(s) 143, 212, 275, 377, 575, 619
CviQI GTAC 2 cut(s) 463, 847
DdeI CTNAG 3 cut(s) 630, 699, 997
DpnI GATC 5 cut(s) 288, 453, 678, 793, 858
DpnII GATC 5 cut(s) 286, 451, 676, 791, 856
DraI TTTAAA 1 cut(s) 838
EaeI YGGCCR 1 cut(s) 516
EagI CGGCCG 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 925
Eco52I CGGCCG 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 3 cut(s) 213, 570, 693
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 43, 263, 885
EcoRV GATATC 1 cut(s) 925
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 6 cut(s) 146, 215, 278, 380, 578, 622
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FaqI GGGAC 1 cut(s) 385
FatI CATG 6 cut(s) 142, 211, 274, 376, 574, 618
FblI GTMKAC 1 cut(s) 568
Fnu4HI GCNGC 6 cut(s) 60, 72, 75, 152, 519, 823
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 6 cut(s) 60, 72, 75, 152, 519, 823
FspBI CTAG 2 cut(s) 20, 596
GluI GCNGC 6 cut(s) 60, 72, 75, 152, 519, 823
GsuI CTGGAG 2 cut(s) 303, 440
HaeIII GGCC 4 cut(s) 16, 325, 518, 897
HapII CCGG 2 cut(s) 403, 803
Hin1II CATG 6 cut(s) 146, 215, 278, 380, 578, 622
HinfI GANTC 3 cut(s) 113, 506, 862
HpaII CCGG 2 cut(s) 403, 803
HphI GGTGA 3 cut(s) 623, 662, 959
Hpy166II GTNNAC 4 cut(s) 494, 569, 781, 950
Hpy188I TCNGA 4 cut(s) 543, 633, 861, 906
Hpy188III TCNNGA 5 cut(s) 103, 231, 290, 457, 928
Hpy8I GTNNAC 4 cut(s) 494, 569, 781, 950
HpyAV CCTTC 1 cut(s) 6
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4V TGCA 7 cut(s) 211, 278, 422, 476, 781, 812, 825
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 80, 139, 148, 596, 710
HpyF3I CTNAG 3 cut(s) 630, 699, 997
Hsp92II CATG 6 cut(s) 146, 215, 278, 380, 578, 622
Kzo9I GATC 5 cut(s) 286, 451, 676, 791, 856
LmnI GCTCC 2 cut(s) 138, 435
Lsp1109I GCAGC 5 cut(s) 71, 83, 86, 163, 809
LweI GCATC 2 cut(s) 92, 799
MaeI CTAG 2 cut(s) 20, 596
MaeIII GTNAC 5 cut(s) 307, 382, 611, 622, 761
MalI GATC 5 cut(s) 288, 453, 678, 793, 858
MboI GATC 5 cut(s) 286, 451, 676, 791, 856
MboII GAAGA 9 cut(s) 219, 406, 475, 566, 576, 671, 686, 745, 786
MflI RGATCY 3 cut(s) 286, 676, 791
MhlI GDGCHC 5 cut(s) 432, 783, 886, 973, 1020
MluCI AATT 5 cut(s) 160, 660, 724, 798, 934
MluI ACGCGT 1 cut(s) 711
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 7 cut(s) 27, 127, 146, 173, 308, 472, 780
MroXI GAANNNNTTC 2 cut(s) 678, 724
MseI TTAA 2 cut(s) 159, 837
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 2 cut(s) 403, 803
MspR9I CCNGG 4 cut(s) 45, 265, 403, 887
MvaI CCWGG 3 cut(s) 45, 265, 887
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 6 cut(s) 68, 80, 139, 148, 596, 710
NciI CCSGG 1 cut(s) 403
NdeII GATC 5 cut(s) 286, 451, 676, 791, 856
NlaIII CATG 6 cut(s) 146, 215, 278, 380, 578, 622
NlaIV GGNNCC 2 cut(s) 883, 1017
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 2 cut(s) 307, 611
NspI RCATGY 3 cut(s) 146, 380, 578
PaeI GCATGC 2 cut(s) 146, 380
PceI AGGCCT 1 cut(s) 16
PdmI GAANNNNTTC 2 cut(s) 678, 724
PfeI GAWTC 2 cut(s) 506, 862
PfoI TCCNGGA 1 cut(s) 401
PkrI GCNGC 6 cut(s) 61, 73, 76, 153, 520, 824
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PsiI TTATAA 1 cut(s) 1030
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 3 cut(s) 43, 263, 885
PspGI CCWGG 3 cut(s) 43, 263, 885
PspN4I GGNNCC 2 cut(s) 883, 1017
PspPI GGNCC 2 cut(s) 323, 896
PstI CTGCAG 2 cut(s) 478, 827
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 3 cut(s) 286, 676, 791
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 2 cut(s) 464, 848
RsaNI GTAC 2 cut(s) 463, 847
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 2 cut(s) 159, 837
SatI GCNGC 6 cut(s) 60, 72, 75, 152, 519, 823
Sau3AI GATC 5 cut(s) 286, 451, 676, 791, 856
Sau96I GGNCC 2 cut(s) 323, 896
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 4 cut(s) 45, 265, 403, 887
SduI GDGCHC 5 cut(s) 432, 783, 886, 973, 1020
SfaNI GCATC 2 cut(s) 92, 799
SfcI CTRYAG 2 cut(s) 474, 823
SmlI CTYRAG 3 cut(s) 137, 299, 387
SmoI CTYRAG 3 cut(s) 137, 299, 387
SphI GCATGC 2 cut(s) 146, 380
Sse9I AATT 5 cut(s) 160, 660, 724, 798, 934
SseBI AGGCCT 1 cut(s) 16
SsiI CCGC 1 cut(s) 519
SspMI CTAG 2 cut(s) 20, 596
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 1 cut(s) 16
StyD4I CCNGG 4 cut(s) 43, 263, 401, 885
TaaI ACNGT 1 cut(s) 566
TaqI TCGA 2 cut(s) 111, 728
TaqII GACCGA 1 cut(s) 326
TasI AATT 5 cut(s) 160, 660, 724, 798, 934
TatI WGTACW 1 cut(s) 846
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 2 cut(s) 506, 862
Tru1I TTAA 2 cut(s) 159, 837
Tru9I TTAA 2 cut(s) 159, 837
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 2 cut(s) 307, 611
TseI GCWGC 5 cut(s) 59, 71, 74, 151, 822
Tsp45I GTSAC 2 cut(s) 307, 611
TspDTI ATGAA 1 cut(s) 746
TspRI CASTG 1 cut(s) 823
VneI GTGCAC 1 cut(s) 779
XapI RAATTY 2 cut(s) 660, 724
XceI RCATGY 3 cut(s) 146, 380, 578
XmiI GTMKAC 1 cut(s) 568
XmnI GAANNNNTTC 2 cut(s) 678, 724
XspI CTAG 2 cut(s) 20, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.