Rw2G010070

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001649
Physical Location & Seq
Forward (+)
10195267 .. 10197646
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010070.1

Sequence Viewer

Length: 1404 bp
ATGGTGGAAGGGGTAGAAAAAGCTTATCACAGAGGGTCGAACACAAGCTGGGGTTGGCGGTTAATTCTGTTACTATATATATCCCTCGTCGCCGCAGCTGCTACCCTCACTCTGCTAAGAAACTTCAACCGCTACTTCCATTCAAGTCAGGTGACTCAGGTCTCGGGTCCACCCGGACCTGTAGTACAAAACTACGCCAACGCTCTTCAACTCGCCACCCAATTCTTCTACGCCAAAAAATCTGGGAAGCTGATGAAGAACCGGGTCACGTGGAGAGGCAACTCGGGGCTCCAAGGTGGGAGTGAGGAGGGGTTGGACTTATCAAAGGGGATGTATGATGCTGGTGATCTTTTAAAGTTTGGGTTTCCTATGGCTTTCACTGCAACAGTGAAGCAATTGGGCCATGCCCATGACTCGCTGAAGTGGATCACTGATTACTTGATCAATGCACATCCTTCCCCAAATGTGCTCTATATCCAGGTGGGTGATCCTGAATTAGACCACAATTGTTGGCAAAGACCTGAAACCATGACAGAGAGAAGGCCTCTAGTTCAAATCAACACATCTTTCCTAGGAACAGAAGTGGAAGCTGAAACAGCAGCAACTATGGCATCAGCATCTCTTGTTTTCAGGAAAATCGACTCCTCATACTCAAAATCGCTCCTCAAGCATGCCGAGCAGCTGTTTAATTTTGCCGATACTTATAGTGGTTCTTATAGTGCCAGCATACCCCAAGTGCAGGGGTTCTACAACTCTTCAGGATATGCAGATGAGCTATTGTGGGCAGCTACCTGGCTCTACCATGCAACTGGAGATCTGGACTACCTCAAGTATGTGACCGAACAGAACGGGCCGACATTTGCTAACTGGGGAAGTCCATCTTGGTTTAGTTGGGATGACAAGCATGCTGCAACTCAAATGTATCGAAAAACTGTAGAGGTCTTCTTGTGTGGACTTATACCTGATTCGCCTACGGCCGCACCTGACAGAACAGACAATGGTCTGATATGGGTGGAAGAATGGAACTGTCTACAACATGCTATGTCTTCAGCCTTTCTAGCCGTTCTCTATAGAGACTACATGGTTACGTCTCAGACTGAAATGCTGTATTGTGATGGGAAAATTTACAAGCCCGAAGATCTTCATTACGCGTTGGGAAAAAATCCGATGAAGATGAGTTATCTTGTAGGGGGATCTTCAATTCCGGTTGATGCAGACACTGGCTACAGAGATGGGTTTAAATGGCTGTACTCCCCCGATCCGAATCCAAATGTAGCTAATGATAGCTATATGGATATCAGGAACAATTCAAAGCAAGGTGAACCAAGCACTTACAATAGTGCTCTCATTGTTGCCCTGCTCTCTGGCTTAGTTTCCACTTCTTCGTTGCCCAAGCACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

467

Amino Acids

52.12

Weight (kDa)

5.85

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 65 - 398 1.1e-100 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1402
AccI GTMKAC 1 cut(s) 1030
AccII CGCG 1 cut(s) 1151
AciI CCGC 4 cut(s) 58, 93, 130, 978
AclWI GGATC 4 cut(s) 434, 482, 1201, 1253
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 1 cut(s) 1122
AcuI CTGAAG 3 cut(s) 440, 741, 1032
AcvI CACGTG 1 cut(s) 270
AfaI GTAC 2 cut(s) 186, 1250
AfiI CCNNNNNNNGG 1 cut(s) 739
AflIII ACRYGT 1 cut(s) 1149
AgsI TTSAA 6 cut(s) 127, 144, 209, 554, 1200, 1311
AjnI CCWGG 2 cut(s) 477, 791
AjuI GAANNNNNNNTTGG 2 cut(s) 865, 897
Alw21I GWGCWC 2 cut(s) 471, 1345
Alw26I GTCTC 3 cut(s) 166, 1068, 1095
AlwI GGATC 4 cut(s) 434, 482, 1201, 1253
AlwNI CAGNNNCTG 1 cut(s) 1220
Ama87I CYCGRG 2 cut(s) 163, 283
AoxI GGCC 4 cut(s) 400, 542, 851, 975
ApeKI GCWGC 6 cut(s) 95, 98, 599, 679, 785, 908
ApoI RAATTY 1 cut(s) 1122
Asp700I GAANNNNTTC 1 cut(s) 1140
AspA2I CCTAGG 1 cut(s) 571
AspS9I GGNCC 4 cut(s) 167, 176, 400, 851
AsuC2I CCSGG 2 cut(s) 174, 263
AsuHPI GGTGA 4 cut(s) 163, 356, 497, 1331
AvaI CYCGRG 2 cut(s) 163, 283
AvaII GGWCC 2 cut(s) 167, 176
AvrII CCTAGG 1 cut(s) 571
BanII GRGCYC 1 cut(s) 291
BbrPI CACGTG 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 934, 1038
Bbv12I GWGCWC 2 cut(s) 471, 1345
BbvI GCAGC 6 cut(s) 85, 107, 611, 691, 797, 895
BccI CCATC 3 cut(s) 886, 1109, 1226
BceAI ACGGC 2 cut(s) 990, 1046
BciT130I CCWGG 2 cut(s) 479, 793
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 2 cut(s) 174, 263
BcoDI GTCTC 3 cut(s) 166, 1068, 1095
BfaI CTAG 3 cut(s) 548, 572, 1058
BfmI CTRYAG 4 cut(s) 180, 933, 1069, 1225
BglII AGATCT 2 cut(s) 814, 1138
BisI GCNGC 8 cut(s) 93, 96, 99, 600, 680, 786, 909, 978
BlnI CCTAGG 1 cut(s) 571
BlsI GCNGC 8 cut(s) 94, 97, 100, 601, 681, 787, 910, 979
Bme1390I CCNGG 4 cut(s) 174, 263, 479, 793
Bme18I GGWCC 2 cut(s) 167, 176
BmeT110I CYCGRG 2 cut(s) 163, 283
BmgT120I GGNCC 4 cut(s) 167, 176, 400, 851
BmiI GGNNCC 2 cut(s) 168, 290
BmrFI CCNGG 4 cut(s) 174, 263, 479, 793
BmrI ACTGGG 1 cut(s) 877
BmsI GCATC 4 cut(s) 328, 620, 626, 1201
BmuI ACTGGG 1 cut(s) 877
BoxI GACNNNNGTC 2 cut(s) 158, 999
BpiI GAAGAC 2 cut(s) 934, 1038
BplI GAGNNNNNCTC 2 cut(s) 529, 561
BpmI CTGGAG 1 cut(s) 831
BpuEI CTTGAG 2 cut(s) 650, 812
BpuMI CCSGG 2 cut(s) 174, 263
BsaAI YACGTR 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 1263
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 292, 571
BsaWI WCCGGW 1 cut(s) 1204
Bsc4I CCNNNNNNNGG 1 cut(s) 739
Bse1I ACTGG 3 cut(s) 814, 872, 1225
Bse8I GATNNNNATC 1 cut(s) 1263
BseBI CCWGG 2 cut(s) 479, 793
BseDI CCNNGG 2 cut(s) 292, 571
BseGI GGATG 3 cut(s) 336, 451, 901
BseJI GATNNNNATC 1 cut(s) 1263
BseLI CCNNNNNNNGG 1 cut(s) 739
BseMII CTCAG 2 cut(s) 170, 1106
BseNI ACTGG 3 cut(s) 814, 872, 1225
BseRI GAGGAG 3 cut(s) 320, 634, 653
BseX3I CGGCCG 1 cut(s) 975
BseXI GCAGC 6 cut(s) 85, 107, 611, 691, 797, 895
BseYI CCCAGC 1 cut(s) 48
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 1151
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 4 cut(s) 402, 544, 853, 977
BsiEI CGRYCG 1 cut(s) 978
BsiHKAI GWGCWC 2 cut(s) 471, 1345
BsiHKCI CYCGRG 2 cut(s) 163, 283
BsiSI CCGG 3 cut(s) 174, 262, 1205
BslI CCNNNNNNNGG 1 cut(s) 739
BsmAI GTCTC 3 cut(s) 166, 1068, 1095
BsmBI CGTCTC 1 cut(s) 1095
BsnI GGCC 4 cut(s) 402, 544, 853, 977
Bso31I GGTCTC 1 cut(s) 166
BsoBI CYCGRG 2 cut(s) 163, 283
Bsp1286I GDGCHC 3 cut(s) 291, 471, 1345
Bsp143I GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
BspACI CCGC 4 cut(s) 58, 93, 130, 978
BspANI GGCC 4 cut(s) 402, 544, 853, 977
BspCNI CTCAG 2 cut(s) 169, 1105
BspFNI CGCG 1 cut(s) 1151
BspLI GGNNCC 2 cut(s) 168, 290
BspPI GGATC 4 cut(s) 434, 482, 1201, 1253
BspQI GCTCTTC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 166
BsrI ACTGG 3 cut(s) 814, 872, 1225
BssECI CCNNGG 2 cut(s) 292, 571
BssMI GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
BssT1I CCWWGG 2 cut(s) 292, 571
Bst2UI CCWGG 2 cut(s) 479, 793
Bst4CI ACNGT 3 cut(s) 388, 934, 1028
Bst6I CTCTTC 2 cut(s) 210, 760
BstBAI YACGTR 1 cut(s) 270
BstC8I GCNNGC 3 cut(s) 672, 724, 906
BstDEI CTNAG 4 cut(s) 116, 156, 1092, 1369
BstF5I GGATG 3 cut(s) 336, 451, 901
BstFNI CGCG 1 cut(s) 1151
BstKTI GATC 8 cut(s) 349, 429, 444, 490, 817, 1141, 1196, 1261
BstMAI GTCTC 3 cut(s) 166, 1068, 1095
BstMBI GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
BstMCI CGRYCG 1 cut(s) 978
BstMWI GCNNNNNNNGC 7 cut(s) 98, 380, 596, 608, 667, 676, 1058
BstNI CCWGG 2 cut(s) 479, 793
BstNSI RCATGY 3 cut(s) 674, 908, 1040
BstPAI GACNNNNGTC 2 cut(s) 158, 999
BstSCI CCNGG 4 cut(s) 172, 261, 477, 791
BstSFI CTRYAG 4 cut(s) 180, 933, 1069, 1225
BstUI CGCG 1 cut(s) 1151
BstV1I GCAGC 6 cut(s) 85, 107, 611, 691, 797, 895
BstV2I GAAGAC 2 cut(s) 934, 1038
BstX2I RGATCY 3 cut(s) 814, 1138, 1193
BstXI CCANNNNNNTGG 1 cut(s) 809
BstYI RGATCY 3 cut(s) 814, 1138, 1193
BstZI CGGCCG 1 cut(s) 975
BsuRI GGCC 4 cut(s) 402, 544, 853, 977
BtsCI GGATG 3 cut(s) 336, 451, 901
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 4 cut(s) 378, 393, 429, 1218
Cac8I GCNNGC 3 cut(s) 672, 724, 906
CaiI CAGNNNCTG 1 cut(s) 1220
Cfr13I GGNCC 4 cut(s) 167, 176, 400, 851
Csp6I GTAC 2 cut(s) 185, 1249
CspCI CAANNNNNGTGG 2 cut(s) 491, 526
CviAII CATG 8 cut(s) 404, 410, 529, 671, 803, 905, 1037, 1081
CviQI GTAC 2 cut(s) 185, 1249
DdeI CTNAG 4 cut(s) 116, 156, 1092, 1369
DpnI GATC 8 cut(s) 348, 428, 443, 489, 816, 1140, 1195, 1260
DpnII GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
DraI TTTAAA 2 cut(s) 354, 1240
EaeI YGGCCR 1 cut(s) 975
EagI CGGCCG 1 cut(s) 975
Eam1104I CTCTTC 2 cut(s) 210, 760
EarI CTCTTC 2 cut(s) 210, 760
EclXI CGGCCG 1 cut(s) 975
Eco130I CCWWGG 2 cut(s) 292, 571
Eco147I AGGCCT 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 1297
Eco47I GGWCC 2 cut(s) 167, 176
Eco52I CGGCCG 1 cut(s) 975
Eco57I CTGAAG 3 cut(s) 440, 741, 1032
Eco72I CACGTG 1 cut(s) 270
Eco88I CYCGRG 2 cut(s) 163, 283
EcoRII CCWGG 2 cut(s) 477, 791
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 2 cut(s) 292, 571
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 292, 571
Esp3I CGTCTC 1 cut(s) 1095
FaeI CATG 8 cut(s) 407, 413, 532, 674, 806, 908, 1040, 1084
FalI AAGNNNNNCTT 2 cut(s) 865, 897
FatI CATG 8 cut(s) 403, 409, 528, 670, 802, 904, 1036, 1080
FbaI TGATCA 1 cut(s) 441
FblI GTMKAC 1 cut(s) 1030
Fnu4HI GCNGC 8 cut(s) 93, 96, 99, 600, 680, 786, 909, 978
FokI GGATG 3 cut(s) 343, 438, 908
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 8 cut(s) 93, 96, 99, 600, 680, 786, 909, 978
FspBI CTAG 3 cut(s) 548, 572, 1058
GluI GCNGC 8 cut(s) 93, 96, 99, 600, 680, 786, 909, 978
GsaI CCCAGC 1 cut(s) 52
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 4 cut(s) 402, 544, 853, 977
HapII CCGG 3 cut(s) 174, 262, 1205
Hin1II CATG 8 cut(s) 407, 413, 532, 674, 806, 908, 1040, 1084
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 5 cut(s) 154, 413, 641, 965, 1264
HpaII CCGG 3 cut(s) 174, 262, 1205
HphI GGTGA 4 cut(s) 163, 356, 497, 1331
Hpy166II GTNNAC 4 cut(s) 170, 953, 1031, 1322
Hpy188I TCNGA 4 cut(s) 1005, 1095, 1167, 1263
Hpy188III TCNNGA 5 cut(s) 491, 631, 759, 818, 1300
Hpy8I GTNNAC 4 cut(s) 170, 953, 1031, 1322
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 465, 534
HpyCH4III ACNGT 3 cut(s) 388, 934, 1028
HpyCH4IV ACGT 2 cut(s) 269, 1088
HpyCH4V TGCA 7 cut(s) 383, 449, 739, 767, 806, 911, 1214
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 380, 596, 608, 667, 676, 1058
HpyF3I CTNAG 4 cut(s) 116, 156, 1092, 1369
HpySE526I ACGT 2 cut(s) 269, 1088
Hsp92II CATG 8 cut(s) 407, 413, 532, 674, 806, 908, 1040, 1084
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 294, 666
Lsp1109I GCAGC 6 cut(s) 85, 107, 611, 691, 797, 895
LweI GCATC 4 cut(s) 328, 620, 626, 1201
MaeI CTAG 3 cut(s) 548, 572, 1058
MaeII ACGT 2 cut(s) 269, 1088
MaeIII GTNAC 5 cut(s) 69, 151, 265, 835, 1084
MalI GATC 8 cut(s) 348, 428, 443, 489, 816, 1140, 1195, 1260
MboI GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
MfeI CAATTG 2 cut(s) 395, 505
MflI RGATCY 3 cut(s) 814, 1138, 1193
MhlI GDGCHC 3 cut(s) 291, 471, 1345
MluCI AATT 9 cut(s) 63, 221, 395, 494, 505, 688, 1122, 1200, 1306
MluI ACGCGT 1 cut(s) 1149
MlyI GAGTC 3 cut(s) 148, 407, 635
MmeI TCCRAC 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 4 cut(s) 62, 353, 687, 1239
MslI CAYNNNNRTG 1 cut(s) 408
MspA1I CMGCKG 2 cut(s) 98, 682
MspI CCGG 3 cut(s) 174, 262, 1205
MspR9I CCNGG 4 cut(s) 174, 263, 479, 793
MunI CAATTG 2 cut(s) 395, 505
MvaI CCWGG 2 cut(s) 479, 793
MvnI CGCG 1 cut(s) 1151
MwoI GCNNNNNNNGC 7 cut(s) 98, 380, 596, 608, 667, 676, 1058
NciI CCSGG 2 cut(s) 174, 263
NdeII GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
NlaIII CATG 8 cut(s) 407, 413, 532, 674, 806, 908, 1040, 1084
NlaIV GGNNCC 2 cut(s) 168, 290
NmeAIII GCCGAG 1 cut(s) 700
NmuCI GTSAC 3 cut(s) 151, 265, 835
NspI RCATGY 3 cut(s) 674, 908, 1040
PaeI GCATGC 2 cut(s) 674, 908
PceI AGGCCT 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 2 cut(s) 965, 1264
PkrI GCNGC 8 cut(s) 94, 97, 100, 601, 681, 787, 910, 979
PleI GAGTC 3 cut(s) 148, 407, 635
PmaCI CACGTG 1 cut(s) 270
PmlI CACGTG 1 cut(s) 270
PpsI GAGTC 3 cut(s) 148, 407, 635
Ppu21I YACGTR 1 cut(s) 270
PshAI GACNNNNGTC 2 cut(s) 158, 999
PsiI TTATAA 1 cut(s) 1402
Psp6I CCWGG 2 cut(s) 477, 791
PspCI CACGTG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 48
PspGI CCWGG 2 cut(s) 477, 791
PspN4I GGNNCC 2 cut(s) 168, 290
PspPI GGNCC 4 cut(s) 167, 176, 400, 851
PstNI CAGNNNCTG 1 cut(s) 1220
PsuI RGATCY 3 cut(s) 814, 1138, 1193
PvuII CAGCTG 2 cut(s) 98, 682
RsaI GTAC 2 cut(s) 186, 1250
RsaNI GTAC 2 cut(s) 185, 1249
RseI CAYNNNNRTG 1 cut(s) 408
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 4 cut(s) 62, 353, 687, 1239
SatI GCNGC 8 cut(s) 93, 96, 99, 600, 680, 786, 909, 978
Sau3AI GATC 8 cut(s) 346, 426, 441, 487, 814, 1138, 1193, 1258
Sau96I GGNCC 4 cut(s) 167, 176, 400, 851
SchI GAGTC 3 cut(s) 148, 407, 635
ScrFI CCNGG 4 cut(s) 174, 263, 479, 793
SduI GDGCHC 3 cut(s) 291, 471, 1345
SfaNI GCATC 4 cut(s) 328, 620, 626, 1201
SfcI CTRYAG 4 cut(s) 180, 933, 1069, 1225
SinI GGWCC 2 cut(s) 167, 176
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 665, 827
SmoI CTYRAG 2 cut(s) 665, 827
SphI GCATGC 2 cut(s) 674, 908
Sse9I AATT 9 cut(s) 63, 221, 395, 494, 505, 688, 1122, 1200, 1306
SseBI AGGCCT 1 cut(s) 544
SsiI CCGC 4 cut(s) 58, 93, 130, 978
SspMI CTAG 3 cut(s) 548, 572, 1058
StuI AGGCCT 1 cut(s) 544
StyD4I CCNGG 4 cut(s) 172, 261, 477, 791
StyI CCWWGG 2 cut(s) 292, 571
TaaI ACNGT 3 cut(s) 388, 934, 1028
TaiI ACGT 2 cut(s) 272, 1091
TaqI TCGA 3 cut(s) 38, 639, 925
TaqII GACCGA 1 cut(s) 854
TasI AATT 9 cut(s) 63, 221, 395, 494, 505, 688, 1122, 1200, 1306
TatI WGTACW 2 cut(s) 184, 1248
TauI GCSGC 2 cut(s) 95, 980
TfiI GAWTC 2 cut(s) 965, 1264
Tru1I TTAA 4 cut(s) 62, 353, 687, 1239
Tru9I TTAA 4 cut(s) 62, 353, 687, 1239
TscAI CASTG 4 cut(s) 385, 393, 436, 1225
TseFI GTSAC 3 cut(s) 151, 265, 835
TseI GCWGC 6 cut(s) 95, 98, 599, 679, 785, 908
Tsp45I GTSAC 3 cut(s) 151, 265, 835
TspDTI ATGAA 3 cut(s) 269, 1133, 1184
TspRI CASTG 4 cut(s) 385, 393, 436, 1225
VpaK11BI GGWCC 2 cut(s) 167, 176
XapI RAATTY 1 cut(s) 1122
XceI RCATGY 3 cut(s) 674, 908, 1040
XmaJI CCTAGG 1 cut(s) 571
XmiI GTMKAC 1 cut(s) 1030
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 3 cut(s) 548, 572, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.