MD08G1014300.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001649
Physical Location & Seq
Forward (+)
1073089 .. 1077013
3925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1014300.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGGAGCTAAATCGAGGTGCAAGGGTTGGTGGGCGTGGCTGCTGGTGCTCGCGATCCTCGGCCTCGTTGTCGGCGCCGTCGTTCTCGCCGTCGTCAAGAAAGTAAAGAAAAACTCCGGCGGCGACGACAGTGCGGTTCCCGGGCCTCCCGGCGCCATTACCCAGAAATACTCCGACGCTCTCAAACTCGCCGTCCAATTCTTCGACGTCCAGAAATCTGGTCAGTTGGTCGATAACAAGATATCGTGGAGAGGGGATTCGGGTCTGAAAGATGGAAGTGATGCGAAGTTGGATTTGTCCAAGGGAATGTATGATGCTGGGGATCACATGAAGTTTGGTTTTCCAATGGCGTATACCGCCACAGTGTTGTCGTGGGCGATTCTTGAGTATGGTGATCAGATGGATGCGGTGAATCAGTTACATACTGCTCAAGCCTCTCTCAAGTGGATCACTGACTTCCTTGTCAATGCTCATCCCAAGGACAATGTGCTCTACGTTCAGGTAGGTGACCCTAAAAAGGACCATGCATGTTGGTATAGGCCGGAAGACATGACCGAGAGTAGGCCAGTCACACAGGTTAACATATCCTCTCCAGGAACGGAGGTTGCAGCTGAAACTGCAGCAGCTATGGCTTCAGCATCTCTGGTCTTCAAGAAAAGTGATGCCAAATATTCAGATACGCTTCTCACGCACGCCAAACAGTTATTTACTTTTGCTGACAAAAACAGAGGTTCTTACAGTGAAAGCATCCCTGAGGTCCAGCAATTTTACAATTCAACGGGATATGGAGATGAGCTTTTGTGGGCAGCAGCCTGGCTTTACCATGCAACTGGGGATAAGGAATACCTTGATTACGTCACAGGGGAGAATGGAGATGAGTTTGCTCAATTTGGTAAACCGACATGGTTCAGTTGGGATAACAAGCTTGCAGGAACCCAGGTTTTGCTTTCAAGGTTGACCTTCTTTGGTGGGAAGGACACCTCAGACAATTCCGGCCTTCGAAAGTACAGGAAAACAGCTGAGGCTGTTATATGTGGCCTCCTGCCAAGCTCGCCAACAGCCACGAAGAGCAGAACAGACAATGGTCTGATCTGGGTCAGTGAATGGAATGCTCTGCAGCATCCGGTTGCTGCTGCTTTCCTAGCTGCTCTTTACAGTGACTACATGCTTACATCTCGCTCCACAAAACTAGAGTGCGATGGAGATACCTATAAGCCATCGGATATTCGGAAATTTGTTAGATCTCAGGCGGATTATGTCTTGGGCGACAATCCTATGAAAATGAGCTTTCTTGTCGGGTACGGGGATAAATATCCCGAATACGTACACCACAGAGGAGCTTCTATCCCGGCTAATCAGAAGACTGGCTGCAAAGATGGATTCAAGTACCTTAACTCAACTGAACCCAACCCTAATATAGCCGTTGGAGCACTTGTCGGCGGGCCATTCCTAAACGAAACATACATTGATGACCGGAACAACTCGAAGCAAGGGGAGCCGAGCACATACAACAGTGCCGTCATTGTTGGCCTTCTGTCAAGTTTGGTGACAACCTCTTCAGCAGTTAAATCCTTCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

527

Amino Acids

57.53

Weight (kDa)

6.33

Isoelectric Point (pI)

27.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 57 - 512 7.4e-159 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 210
AccB1I GGYRCC 2 cut(s) 74, 152
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 53
AciI CCGC 6 cut(s) 120, 134, 357, 407, 1250, 1440
AclWI GGATC 3 cut(s) 49, 330, 455
AcsI RAATTY 1 cut(s) 1232
AcuI CTGAAG 2 cut(s) 618, 1542
AcyI GRCGYC 3 cut(s) 75, 153, 207
AfaI GTAC 4 cut(s) 1007, 1301, 1326, 1388
AfiI CCNNNNNNNGG 2 cut(s) 517, 561
AgsI TTSAA 4 cut(s) 652, 777, 951, 1384
AjnI CCWGG 3 cut(s) 592, 812, 936
Alw21I GWGCWC 4 cut(s) 51, 492, 1432, 1505
AlwI GGATC 3 cut(s) 49, 330, 455
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 8 cut(s) 61, 143, 539, 563, 994, 1036, 1442, 1528
ApoI RAATTY 1 cut(s) 1232
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AspLEI GCGC 2 cut(s) 77, 155
AspS9I GGNCC 4 cut(s) 143, 520, 757, 1442
AsuC2I CCSGG 4 cut(s) 141, 142, 150, 1349
AsuHPI GGTGA 5 cut(s) 404, 421, 518, 1558, 1567
AsuII TTCGAA 1 cut(s) 1000
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 2 cut(s) 520, 757
AxyI CCTNAGG 1 cut(s) 753
BanI GGYRCC 2 cut(s) 74, 152
BbsI GAAGAC 3 cut(s) 552, 640, 1367
Bbv12I GWGCWC 4 cut(s) 51, 492, 1432, 1505
BbvCI CCTCAGC 1 cut(s) 1020
BccI CCATC 5 cut(s) 266, 394, 1193, 1225, 1370
BceAI ACGGC 5 cut(s) 62, 74, 176, 1406, 1502
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 3 cut(s) 594, 814, 938
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 4 cut(s) 141, 142, 150, 1349
BfaI CTAG 3 cut(s) 1142, 1190, 1579
BfmI CTRYAG 2 cut(s) 618, 1115
BfoI RGCGCY 2 cut(s) 78, 156
BglII AGATCT 1 cut(s) 1241
Bme1390I CCNGG 7 cut(s) 141, 142, 150, 594, 814, 938, 1349
Bme18I GGWCC 2 cut(s) 520, 757
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 4 cut(s) 143, 520, 757, 1442
BmiI GGNNCC 5 cut(s) 76, 138, 154, 934, 1497
BmrFI CCNGG 7 cut(s) 141, 142, 150, 594, 814, 938, 1349
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 7 cut(s) 271, 304, 394, 647, 652, 756, 1129
BmuI ACTGGG 1 cut(s) 840
BoxI GACNNNNGTC 1 cut(s) 1083
BpiI GAAGAC 3 cut(s) 552, 640, 1367
BpmI CTGGAG 1 cut(s) 576
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 1000
BpuEI CTTGAG 3 cut(s) 404, 414, 425
BpuMI CCSGG 4 cut(s) 141, 142, 150, 1349
BsaAI YACGTR 1 cut(s) 1324
BsaBI GATNNNNATC 1 cut(s) 1311
BsaHI GRCGYC 3 cut(s) 75, 153, 207
BsaJI CCNNGG 5 cut(s) 58, 140, 300, 477, 936
BsaWI WCCGGW 2 cut(s) 1123, 1473
BsaXI ACNNNNNCTCC 2 cut(s) 1419, 1449
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 561
Bse1I ACTGG 3 cut(s) 566, 835, 1369
Bse21I CCTNAGG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 1311
BseBI CCWGG 3 cut(s) 594, 814, 938
BseDI CCNNGG 5 cut(s) 58, 140, 300, 477, 936
BseGI GGATG 4 cut(s) 409, 472, 747, 1120
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 2 cut(s) 517, 561
BseMII CTCAG 4 cut(s) 744, 996, 1011, 1259
BseNI ACTGG 3 cut(s) 566, 835, 1369
BseRI GAGGAG 1 cut(s) 1350
BseYI CCCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 8 cut(s) 63, 145, 541, 565, 996, 1038, 1444, 1530
BshNI GGYRCC 2 cut(s) 74, 152
BsiHKAI GWGCWC 4 cut(s) 51, 492, 1432, 1505
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 8 cut(s) 117, 141, 150, 542, 993, 1124, 1349, 1474
BslI CCNNNNNNNGG 2 cut(s) 517, 561
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 8 cut(s) 63, 145, 541, 565, 996, 1038, 1444, 1530
BsoBI CYCGRG 1 cut(s) 140
Bsp119I TTCGAA 1 cut(s) 1000
Bsp1286I GDGCHC 4 cut(s) 51, 492, 1432, 1505
Bsp143I GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
Bsp68I TCGCGA 1 cut(s) 53
BspACI CCGC 6 cut(s) 120, 134, 357, 407, 1250, 1440
BspANI GGCC 8 cut(s) 63, 145, 541, 565, 996, 1038, 1444, 1530
BspCNI CTCAG 4 cut(s) 745, 995, 1012, 1258
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 5 cut(s) 76, 138, 154, 934, 1497
BspMAI CTGCAG 2 cut(s) 622, 1119
BspPI GGATC 3 cut(s) 49, 330, 455
BspQI GCTCTTC 1 cut(s) 1061
BspT104I TTCGAA 1 cut(s) 1000
BspT107I GGYRCC 2 cut(s) 74, 152
BsrI ACTGG 3 cut(s) 566, 835, 1369
BssECI CCNNGG 5 cut(s) 58, 140, 300, 477, 936
BssMI GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
BssNAI GTATAC 1 cut(s) 354
BssNI GRCGYC 3 cut(s) 75, 153, 207
BssT1I CCWWGG 2 cut(s) 300, 477
Bst1107I GTATAC 1 cut(s) 354
Bst2UI CCWGG 3 cut(s) 594, 814, 938
Bst4CI ACNGT 6 cut(s) 131, 364, 702, 740, 1157, 1514
Bst6I CTCTTC 2 cut(s) 1061, 1561
BstACI GRCGYC 3 cut(s) 75, 153, 207
BstBAI YACGTR 1 cut(s) 1324
BstBI TTCGAA 1 cut(s) 1000
BstC8I GCNNGC 5 cut(s) 51, 693, 927, 1052, 1442
BstDEI CTNAG 4 cut(s) 753, 982, 1020, 1245
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 4 cut(s) 409, 472, 747, 1120
BstFNI CGCG 1 cut(s) 53
BstH2I RGCGCY 2 cut(s) 78, 156
BstHHI GCGC 2 cut(s) 77, 155
BstKTI GATC 6 cut(s) 57, 325, 397, 450, 1092, 1244
BstMBI GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
BstMWI GCNNNNNNNGC 9 cut(s) 46, 356, 617, 629, 688, 1051, 1142, 1427, 1495
BstNI CCWGG 3 cut(s) 594, 814, 938
BstNSI RCATGY 2 cut(s) 531, 1168
BstPAI GACNNNNGTC 1 cut(s) 1083
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 7 cut(s) 139, 140, 148, 592, 812, 936, 1347
BstSFI CTRYAG 2 cut(s) 618, 1115
BstSNI TACGTA 1 cut(s) 1324
BstUI CGCG 1 cut(s) 53
BstV2I GAAGAC 3 cut(s) 552, 640, 1367
BstX2I RGATCY 1 cut(s) 1241
BstXI CCANNNNNNTGG 2 cut(s) 218, 830
BstYI RGATCY 1 cut(s) 1241
BstZ17I GTATAC 1 cut(s) 354
Bsu36I CCTNAGG 1 cut(s) 753
BsuRI GGCC 8 cut(s) 63, 145, 541, 565, 996, 1038, 1444, 1530
BtgZI GCGATG 1 cut(s) 1212
BtsCI GGATG 4 cut(s) 409, 472, 747, 1120
BtsIMutI CAGTG 7 cut(s) 136, 369, 450, 745, 1105, 1162, 1519
BtuMI TCGCGA 1 cut(s) 53
Cac8I GCNNGC 5 cut(s) 51, 693, 927, 1052, 1442
CfoI GCGC 2 cut(s) 77, 155
Cfr13I GGNCC 4 cut(s) 143, 520, 757, 1442
Cfr9I CCCGGG 1 cut(s) 140
CseI GACGC 1 cut(s) 185
Csp6I GTAC 4 cut(s) 1006, 1300, 1325, 1387
CviAII CATG 7 cut(s) 328, 524, 528, 550, 824, 903, 1165
CviQI GTAC 4 cut(s) 1006, 1300, 1325, 1387
DdeI CTNAG 4 cut(s) 753, 982, 1020, 1245
DinI GGCGCC 2 cut(s) 76, 154
DpnI GATC 6 cut(s) 56, 324, 396, 449, 1091, 1243
DpnII GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 1061, 1561
EarI CTCTTC 2 cut(s) 1061, 1561
EciI GGCGGA 1 cut(s) 1265
Eco105I TACGTA 1 cut(s) 1324
Eco130I CCWWGG 2 cut(s) 300, 477
Eco32I GATATC 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 520, 757
Eco57I CTGAAG 2 cut(s) 618, 1542
Eco81I CCTNAGG 1 cut(s) 753
Eco88I CYCGRG 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 506
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 3 cut(s) 592, 812, 936
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 2 cut(s) 300, 477
EcoT22I ATGCAT 1 cut(s) 529
EgeI GGCGCC 2 cut(s) 76, 154
EheI GGCGCC 2 cut(s) 76, 154
ErhI CCWWGG 2 cut(s) 300, 477
FaeI CATG 7 cut(s) 331, 527, 531, 553, 827, 906, 1168
FatI CATG 7 cut(s) 327, 523, 527, 549, 823, 902, 1164
FauI CCCGC 1 cut(s) 1433
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 353
FokI GGATG 4 cut(s) 416, 459, 734, 1107
FspBI CTAG 3 cut(s) 1142, 1190, 1579
GlaI GCGC 2 cut(s) 76, 154
GsaI CCCAGC 1 cut(s) 321
GsuI CTGGAG 1 cut(s) 576
HaeII RGCGCY 2 cut(s) 78, 156
HaeIII GGCC 8 cut(s) 63, 145, 541, 565, 996, 1038, 1444, 1530
HapII CCGG 8 cut(s) 117, 141, 150, 542, 993, 1124, 1349, 1474
HgaI GACGC 1 cut(s) 185
HhaI GCGC 2 cut(s) 77, 155
Hin1I GRCGYC 3 cut(s) 75, 153, 207
Hin1II CATG 7 cut(s) 331, 527, 531, 553, 827, 906, 1168
Hin6I GCGC 2 cut(s) 75, 153
HinP1I GCGC 2 cut(s) 75, 153
HincII GTYRAC 2 cut(s) 580, 957
HindII GTYRAC 2 cut(s) 580, 957
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 4 cut(s) 257, 379, 412, 1380
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 8 cut(s) 117, 141, 150, 542, 993, 1124, 1349, 1474
HphI GGTGA 5 cut(s) 404, 421, 518, 1558, 1567
Hpy166II GTNNAC 5 cut(s) 354, 580, 896, 957, 1327
Hpy188I TCNGA 9 cut(s) 175, 267, 399, 676, 985, 1089, 1222, 1230, 1359
Hpy188III TCNNGA 6 cut(s) 52, 97, 211, 383, 652, 1316
Hpy8I GTNNAC 5 cut(s) 354, 580, 896, 957, 1327
Hpy99I CGWCG 5 cut(s) 83, 95, 128, 179, 209
HpyAV CCTTC 4 cut(s) 967, 970, 1007, 1541
HpyCH4III ACNGT 6 cut(s) 131, 364, 702, 740, 1157, 1514
HpyCH4IV ACGT 4 cut(s) 207, 495, 855, 1323
HpyCH4V TGCA 8 cut(s) 21, 527, 608, 620, 827, 929, 1117, 1371
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 356, 617, 629, 688, 1051, 1142, 1427, 1495
HpyF3I CTNAG 4 cut(s) 753, 982, 1020, 1245
HpySE526I ACGT 4 cut(s) 207, 495, 855, 1323
Hsp92I GRCGYC 3 cut(s) 75, 153, 207
Hsp92II CATG 7 cut(s) 331, 527, 531, 553, 827, 906, 1168
HspAI GCGC 2 cut(s) 75, 153
KasI GGCGCC 2 cut(s) 74, 152
Ksp22I TGATCA 1 cut(s) 394
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
LguI GCTCTTC 1 cut(s) 1061
LmnI GCTCC 5 cut(s) 5, 1184, 1337, 1427, 1495
LweI GCATC 7 cut(s) 271, 304, 394, 647, 652, 756, 1129
MaeI CTAG 3 cut(s) 1142, 1190, 1579
MaeII ACGT 4 cut(s) 207, 495, 855, 1323
MaeIII GTNAC 6 cut(s) 417, 506, 568, 856, 1157, 1546
MalI GATC 6 cut(s) 56, 324, 396, 449, 1091, 1243
MboI GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
MboII GAAGA 6 cut(s) 193, 557, 640, 1078, 1372, 1548
MflI RGATCY 1 cut(s) 1241
MhlI GDGCHC 4 cut(s) 51, 492, 1432, 1505
MluCI AATT 6 cut(s) 197, 764, 772, 887, 988, 1232
Mly113I GGCGCC 2 cut(s) 75, 153
MmeI TCCRAC 3 cut(s) 198, 270, 1405
Mph1103I ATGCAT 1 cut(s) 529
MseI TTAA 3 cut(s) 579, 1392, 1566
MspA1I CMGCKG 2 cut(s) 611, 1019
MspI CCGG 8 cut(s) 117, 141, 150, 542, 993, 1124, 1349, 1474
MspR9I CCNGG 7 cut(s) 141, 142, 150, 594, 814, 938, 1349
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 3 cut(s) 594, 814, 938
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 9 cut(s) 46, 356, 617, 629, 688, 1051, 1142, 1427, 1495
NarI GGCGCC 2 cut(s) 75, 153
NciI CCSGG 4 cut(s) 141, 142, 150, 1349
NdeII GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
NlaIII CATG 7 cut(s) 331, 527, 531, 553, 827, 906, 1168
NlaIV GGNNCC 5 cut(s) 76, 138, 154, 934, 1497
NmeAIII GCCGAG 2 cut(s) 39, 1524
NmuCI GTSAC 5 cut(s) 506, 568, 856, 1157, 1546
NruI TCGCGA 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 2 cut(s) 531, 1168
NspV TTCGAA 1 cut(s) 1000
PciSI GCTCTTC 1 cut(s) 1061
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 87
PctI GAATGC 1 cut(s) 1114
PfeI GAWTC 4 cut(s) 257, 379, 412, 1380
PfoI TCCNGGA 1 cut(s) 592
PluTI GGCGCC 2 cut(s) 78, 156
Ppu21I YACGTR 1 cut(s) 1324
PshAI GACNNNNGTC 1 cut(s) 1083
Psp6I CCWGG 3 cut(s) 592, 812, 936
PspEI GGTNACC 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 317
PspGI CCWGG 3 cut(s) 592, 812, 936
PspN4I GGNNCC 5 cut(s) 76, 138, 154, 934, 1497
PspPI GGNCC 4 cut(s) 143, 520, 757, 1442
PstI CTGCAG 2 cut(s) 622, 1119
PsuI RGATCY 1 cut(s) 1241
PvuII CAGCTG 2 cut(s) 611, 1019
RruI TCGCGA 1 cut(s) 53
RsaI GTAC 4 cut(s) 1007, 1301, 1326, 1388
RsaNI GTAC 4 cut(s) 1006, 1300, 1325, 1387
SapI GCTCTTC 1 cut(s) 1061
SaqAI TTAA 3 cut(s) 579, 1392, 1566
Sau3AI GATC 6 cut(s) 54, 322, 394, 447, 1089, 1241
Sau96I GGNCC 4 cut(s) 143, 520, 757, 1442
ScrFI CCNGG 7 cut(s) 141, 142, 150, 594, 814, 938, 1349
SduI GDGCHC 4 cut(s) 51, 492, 1432, 1505
SfaNI GCATC 7 cut(s) 271, 304, 394, 647, 652, 756, 1129
SfcI CTRYAG 2 cut(s) 618, 1115
SfoI GGCGCC 2 cut(s) 76, 154
SfuI TTCGAA 1 cut(s) 1000
SinI GGWCC 2 cut(s) 520, 757
SmaI CCCGGG 1 cut(s) 142
SmlI CTYRAG 3 cut(s) 383, 429, 440
SmoI CTYRAG 3 cut(s) 383, 429, 440
SnaBI TACGTA 1 cut(s) 1324
Sse9I AATT 6 cut(s) 197, 764, 772, 887, 988, 1232
SsiI CCGC 6 cut(s) 120, 134, 357, 407, 1250, 1440
SspDI GGCGCC 2 cut(s) 74, 152
SspI AATATT 1 cut(s) 671
SspMI CTAG 3 cut(s) 1142, 1190, 1579
StyD4I CCNGG 7 cut(s) 139, 140, 148, 592, 812, 936, 1347
StyI CCWWGG 2 cut(s) 300, 477
TaaI ACNGT 6 cut(s) 131, 364, 702, 740, 1157, 1514
TaiI ACGT 4 cut(s) 210, 498, 858, 1326
TaqI TCGA 5 cut(s) 14, 204, 231, 1000, 1484
TaqII GACCGA 1 cut(s) 569
TasI AATT 6 cut(s) 197, 764, 772, 887, 988, 1232
TatI WGTACW 1 cut(s) 1005
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 4 cut(s) 257, 379, 412, 1380
Tru1I TTAA 3 cut(s) 579, 1392, 1566
Tru9I TTAA 3 cut(s) 579, 1392, 1566
TscAI CASTG 7 cut(s) 136, 369, 457, 745, 1105, 1162, 1519
TseFI GTSAC 5 cut(s) 506, 568, 856, 1157, 1546
Tsp45I GTSAC 5 cut(s) 506, 568, 856, 1157, 1546
TspDTI ATGAA 2 cut(s) 344, 1292
TspGWI ACGGA 1 cut(s) 614
TspMI CCCGGG 1 cut(s) 140
TspRI CASTG 7 cut(s) 136, 369, 457, 745, 1105, 1162, 1519
VpaK11BI GGWCC 2 cut(s) 520, 757
XapI RAATTY 1 cut(s) 1232
XceI RCATGY 2 cut(s) 531, 1168
XmaI CCCGGG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 3 cut(s) 1142, 1190, 1579
ZraI GACGTC 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.