AT1G20050

Emopamil binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004072
Physical Location & Seq
Forward (+)
6949043 .. 6950509
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20050.1

Sequence Viewer

Length: 672 bp
ATGGAGGAGTTGGCGCATCCGTACGTTCCGAGAGATCTGAACCTACCCGGATACGTACCAATCTCAATGTCAATGTCCTCCATCGTCTCTATCTACCTCGGTTCTTCCCTCCTTGTTGTCTCCCTCGTCTGGCTTCTCTTCGGGAGGAAGAAAGCTAAACTTGATAAGTTGCTTATGTGTTGGTGGACATTCACTGGTCTCACTCATGTTATTCTCGAGGGCTATTTCGTTTTCTCCCCTGAGTTTTTCAAGGACAACACTTCTGCTTATCTTGCTGAAGTTTGGAAAGAATATAGCAAAGGTGATTCGAGATACGTAGGTAGAGATTCTGCAGTTGTATCTGTTGAAGGGATCACTGCTGTTATCGTTGGCCCAGCTTCTCTCCTAGCTATTTATGCCATTGCTAAGGAGAAGTCGTATAGCTACGTGCTTCAGCTTGCGATTTCGGTTTGCCAGCTCTACGGATGTTTGGTTTATTTCATTACTGCTATCTTGGAAGGAGACAACTTTGCCACAAACTCTTTCTACTATTACTCCTACTACATCGGGGCTAACTGTTGGTGGGTCTTGATACCTTCACTCATTTCTTTCCGTTGCTGGAAAAAGATTTGTGCAGCTGCTGCCATTGCCAACAACAACGTCGAGACAAAGACGAAGAAGAAAACCCGTTGA

Protein Analysis

223

Amino Acids

25.15

Weight (kDa)

8.77

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 91 - 199 2.3e-22 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 297, 416
AfaI GTAC 2 cut(s) 23, 57
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 2 cut(s) 250, 347
AluBI AGCT 7 cut(s) 155, 377, 389, 423, 436, 457, 617
AluI AGCT 7 cut(s) 155, 377, 389, 423, 436, 457, 617
Alw26I GTCTC 5 cut(s) 91, 124, 203, 495, 638
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 3 cut(s) 614, 617, 620
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 314
AvaI CYCGRG 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BbvI GCAGC 3 cut(s) 604, 607, 626
BccI CCATC 1 cut(s) 89
BciVI GTATCC 1 cut(s) 44
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 5 cut(s) 91, 124, 203, 495, 638
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 1 cut(s) 330
BfuI GTATCC 1 cut(s) 44
BglII AGATCT 1 cut(s) 34
BisI GCNGC 3 cut(s) 615, 618, 621
BlsI GCNGC 3 cut(s) 616, 619, 622
Bme1390I CCNGG 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 48
BsaAI YACGTR 3 cut(s) 55, 316, 427
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 97
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 518, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 2 cut(s) 399, 624
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 2 cut(s) 16, 470
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMI GCAATG 2 cut(s) 399, 624
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 604, 607, 626
BseYI CCCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 633
BshFI GGCC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 1 cut(s) 48
BsiWI CGTACG 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 5 cut(s) 91, 124, 203, 495, 638
BsmBI CGTCTC 1 cut(s) 91
BsnI GGCC 1 cut(s) 372
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 2 cut(s) 34, 351
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 2 cut(s) 399, 624
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 2 cut(s) 34, 351
Bst4CI ACNGT 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBAI YACGTR 3 cut(s) 55, 316, 427
BstC8I GCNNGC 2 cut(s) 438, 455
BstDEI CTNAG 2 cut(s) 240, 405
BstF5I GGATG 2 cut(s) 16, 470
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 2 cut(s) 37, 354
BstMAI GTCTC 5 cut(s) 91, 124, 203, 495, 638
BstMBI GATC 2 cut(s) 34, 351
BstMWI GCNNNNNNNGC 4 cut(s) 272, 395, 620, 626
BstSCI CCNGG 1 cut(s) 46
BstSFI CTRYAG 1 cut(s) 330
BstSNI TACGTA 2 cut(s) 55, 316
BstV1I GCAGC 3 cut(s) 604, 607, 626
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 44
BsuRI GGCC 1 cut(s) 372
BtsCI GGATG 2 cut(s) 16, 470
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 192, 354
Cac8I GCNNGC 2 cut(s) 438, 455
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 371
Csp6I GTAC 2 cut(s) 22, 56
CviAII CATG 1 cut(s) 206
CviQI GTAC 2 cut(s) 22, 56
DdeI CTNAG 2 cut(s) 240, 405
DpnI GATC 2 cut(s) 36, 353
DpnII GATC 2 cut(s) 34, 351
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco105I TACGTA 2 cut(s) 55, 316
Eco31I GGTCTC 1 cut(s) 203
Eco57I CTGAAG 2 cut(s) 297, 416
Eco88I CYCGRG 1 cut(s) 215
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 1 cut(s) 209
FaiI YATR 5 cut(s) 176, 207, 294, 396, 420
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FatI CATG 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 615, 618, 621
FokI GGATG 2 cut(s) 3, 477
Fsp4HI GCNGC 3 cut(s) 615, 618, 621
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 615, 618, 621
GsaI CCCAGC 1 cut(s) 377
HaeIII GGCC 1 cut(s) 372
HapII CCGG 1 cut(s) 48
HhaI GCGC 1 cut(s) 16
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HinfI GANTC 2 cut(s) 305, 326
HpaII CCGG 1 cut(s) 48
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 2 cut(s) 30, 39
Hpy188III TCNNGA 5 cut(s) 142, 215, 309, 568, 643
Hpy8I GTNNAC 1 cut(s) 186
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 3 cut(s) 341, 491, 585
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 5 cut(s) 24, 54, 315, 426, 639
HpyCH4V TGCA 2 cut(s) 332, 614
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 395, 620, 626
HpyF3I CTNAG 2 cut(s) 240, 405
HpySE526I ACGT 5 cut(s) 24, 54, 315, 426, 639
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 2 cut(s) 34, 351
LpnPI CCDG 7 cut(s) 61, 115, 180, 252, 387, 467, 583
Lsp1109I GCAGC 3 cut(s) 604, 607, 626
LweI GCATC 1 cut(s) 25
MaeI CTAG 1 cut(s) 386
MaeII ACGT 5 cut(s) 24, 54, 315, 426, 639
MalI GATC 2 cut(s) 36, 353
MboI GATC 2 cut(s) 34, 351
MboII GAAGA 5 cut(s) 96, 130, 160, 667, 670
MflI RGATCY 1 cut(s) 34
MnlI CCTC 6 cut(s) 88, 107, 119, 134, 138, 211
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 48
MwoI GCNNNNNNNGC 4 cut(s) 272, 395, 620, 626
NciI CCSGG 1 cut(s) 48
NdeII GATC 2 cut(s) 34, 351
NlaIII CATG 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 215
PfeI GAWTC 2 cut(s) 305, 326
Pfl23II CGTACG 1 cut(s) 21
PkrI GCNGC 3 cut(s) 616, 619, 622
Ppu21I YACGTR 3 cut(s) 55, 316, 427
PspFI CCCAGC 1 cut(s) 373
PspLI CGTACG 1 cut(s) 21
PspPI GGNCC 1 cut(s) 371
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 23, 57
RsaNI GTAC 2 cut(s) 22, 56
SatI GCNGC 3 cut(s) 615, 618, 621
Sau3AI GATC 2 cut(s) 34, 351
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 48
SfaNI GCATC 1 cut(s) 25
SfcI CTRYAG 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SnaBI TACGTA 2 cut(s) 55, 316
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 5 cut(s) 27, 57, 318, 429, 642
TaqI TCGA 3 cut(s) 216, 308, 642
TfiI GAWTC 2 cut(s) 305, 326
TscAI CASTG 2 cut(s) 199, 361
TseI GCWGC 3 cut(s) 614, 617, 620
TspDTI ATGAA 1 cut(s) 469
TspGWI ACGGA 3 cut(s) 9, 477, 581
TspRI CASTG 2 cut(s) 199, 361
XhoI CTCGAG 1 cut(s) 215
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.