pycom16g11380

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004072
Physical Location & Seq
Forward (+)
7938923 .. 7939704
782 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g11380.1

Sequence Viewer

Length: 732 bp
ATGTCCACAGCTGAGAAAATCGGGCACCTGAAAGACTGCAGCTCCATAGAACTGAAGGTAATCTCCTGCAAGGATCTCAGTGCCTTCAATTTCTTCAACAGGCTGTCCGTCTACGCTGCTGTCTCCATCTTCAACGACGAAGCGAAGAAAGAAGAACAGAAGAAACATCTGCAGCAGCGCCAGAAGACGCCGGTTGATAGAGAAGGGGACTGCAATCCTGAATGGAACCACCCCATGCAATTCGACACGAAGGACTTGTCCCTGGTTGACGAGTTTGATAATCTCTTTGTAGAATTCGACATACGATGTGACAGCGTCTTTGGCAAGAAGAGCATTGGAAAAGTCCGGGTGCCGTTCGCGGACTTGATCGAAGATCAGTGCAATGAAGCGGTCAGGTTCGTGAGCTACCAGGTTCGGACAAGCGATGGTAAGCCTAATGGGGTCTTGAACTTTTCTTACAAGGTGATCAAGAAGAACAATAAAGTTGGAATCCATGATTCCCAGGAATCCGATTCCTCTCCAATCTCTTTACCCCACCTTGGACGTGCAAGTGCAATCCCGGGATATAAACGTATCCTATCCTTCGCTATCCGATGTTCGGGCTCCTTTGCCGACAATTTCAGTACCTTCTCCCAAGTTCAATTGCCATTGGAGGCCAGAACCTTACAACATGAAGACGCTGCCATCTCAGCTCCCGTTTTCTCCGCCAGCAACTGGGCCTGCAGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

244

Amino Acids

27.31

Weight (kDa)

6.6

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 16 - 125 2.8e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 714
AccB1I GGYRCC 2 cut(s) 24, 349
AccB7I CCANNNNNTGG 1 cut(s) 714
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 359
AciI CCGC 3 cut(s) 359, 389, 705
AclWI GGATC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 188
AfaI GTAC 1 cut(s) 625
AfiI CCNNNNNNNGG 3 cut(s) 539, 598, 714
AgsI TTSAA 5 cut(s) 88, 97, 133, 448, 641
AjiI CACGTC 1 cut(s) 545
AjnI CCWGG 3 cut(s) 261, 408, 501
AluBI AGCT 4 cut(s) 11, 42, 405, 692
AluI AGCT 4 cut(s) 11, 42, 405, 692
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 81
AlwNI CAGNNNCTG 1 cut(s) 714
Ama87I CYCGRG 1 cut(s) 559
AoxI GGCC 2 cut(s) 654, 717
ApeKI GCWGC 5 cut(s) 39, 116, 172, 175, 680
ApoI RAATTY 1 cut(s) 293
ArsI GACNNNNNNTTYG 4 cut(s) 242, 274, 302, 334
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 717, 726
AsuC2I CCSGG 3 cut(s) 347, 560, 561
AsuHPI GGTGA 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 559
AvaII GGWCC 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 2 cut(s) 24, 349
BanII GRGCYC 1 cut(s) 605
BbsI GAAGAC 2 cut(s) 191, 681
BbvI GCAGC 5 cut(s) 51, 103, 184, 187, 667
BccI CCATC 3 cut(s) 134, 419, 692
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 3 cut(s) 263, 410, 503
BciVI GTATCC 1 cut(s) 584
BclI TGATCA 1 cut(s) 465
BcnI CCSGG 3 cut(s) 347, 560, 561
BcoDI GTCTC 1 cut(s) 127
BfmI CTRYAG 3 cut(s) 37, 170, 721
BfoI RGCGCY 1 cut(s) 181
BfuAI ACCTGC 1 cut(s) 714
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 5 cut(s) 40, 117, 173, 176, 681
BlsI GCNGC 5 cut(s) 41, 118, 174, 177, 682
Bme1390I CCNGG 6 cut(s) 263, 347, 410, 503, 560, 561
Bme18I GGWCC 1 cut(s) 726
BmeT110I CYCGRG 1 cut(s) 559
BmgBI CACGTC 1 cut(s) 545
BmgT120I GGNCC 2 cut(s) 717, 726
BmiI GGNNCC 4 cut(s) 26, 227, 351, 604
BmrFI CCNGG 6 cut(s) 263, 347, 410, 503, 560, 561
BmrI ACTGGG 1 cut(s) 724
BmuI ACTGGG 1 cut(s) 724
BpiI GAAGAC 2 cut(s) 191, 681
BpuMI CCSGG 3 cut(s) 347, 560, 561
BsaHI GRCGYC 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 261, 501, 538, 559
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 539, 598, 714
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 3 cut(s) 263, 410, 503
BseDI CCNNGG 4 cut(s) 261, 501, 538, 559
BseLI CCNNNNNNNGG 3 cut(s) 539, 598, 714
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 91, 702
BseNI ACTGG 1 cut(s) 719
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 5 cut(s) 51, 103, 184, 187, 667
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 656, 719
BshNI GGYRCC 2 cut(s) 24, 349
BsiHKCI CYCGRG 1 cut(s) 559
BsiSI CCGG 3 cut(s) 191, 346, 560
BslFI GGGAC 2 cut(s) 221, 244
BslI CCNNNNNNNGG 3 cut(s) 539, 598, 714
BsmAI GTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 221, 244
BsnI GGCC 2 cut(s) 656, 719
BsoBI CYCGRG 1 cut(s) 559
Bsp1286I GDGCHC 2 cut(s) 27, 605
Bsp143I GATC 4 cut(s) 73, 366, 373, 465
BspACI CCGC 3 cut(s) 359, 389, 705
BspANI GGCC 2 cut(s) 656, 719
BspCNI CTCAG 3 cut(s) 4, 90, 701
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 26, 227, 351, 604
BspMAI CTGCAG 3 cut(s) 41, 174, 725
BspMI ACCTGC 1 cut(s) 714
BspPI GGATC 1 cut(s) 81
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 24, 349
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 4 cut(s) 261, 501, 538, 559
BssMI GATC 4 cut(s) 73, 366, 373, 465
BssNI GRCGYC 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 538
Bst2UI CCWGG 3 cut(s) 263, 410, 503
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 709, 721
BstDEI CTNAG 3 cut(s) 12, 77, 688
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 4 cut(s) 76, 369, 376, 468
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 4 cut(s) 73, 366, 373, 465
BstMWI GCNNNNNNNGC 3 cut(s) 321, 330, 689
BstNI CCWGG 3 cut(s) 263, 410, 503
BstSCI CCNGG 6 cut(s) 261, 345, 408, 501, 558, 559
BstSFI CTRYAG 3 cut(s) 37, 170, 721
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 5 cut(s) 51, 103, 184, 187, 667
BstV2I GAAGAC 2 cut(s) 191, 681
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 2 cut(s) 656, 719
BtgZI GCGATG 1 cut(s) 438
BtrI CACGTC 1 cut(s) 545
BtsIMutI CAGTG 2 cut(s) 85, 383
BveI ACCTGC 1 cut(s) 714
Cac8I GCNNGC 2 cut(s) 709, 721
CaiI CAGNNNCTG 1 cut(s) 714
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 717, 726
Cfr9I CCCGGG 1 cut(s) 559
CseI GACGC 3 cut(s) 196, 304, 686
CsiI ACCWGGT 1 cut(s) 408
Csp6I GTAC 1 cut(s) 624
CviAII CATG 3 cut(s) 235, 494, 671
CviJI RGCY 9 cut(s) 11, 42, 103, 405, 433, 603, 656, 692, 719
CviKI_1 RGCY 9 cut(s) 11, 42, 103, 405, 433, 603, 656, 692, 719
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 3 cut(s) 12, 77, 688
DpnI GATC 4 cut(s) 75, 368, 375, 467
DpnII GATC 4 cut(s) 73, 366, 373, 465
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
EciI GGCGGA 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 605
Eco47I GGWCC 1 cut(s) 726
Eco57I CTGAAG 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 559
EcoO109I RGGNCCY 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 261, 408, 501
EcoT14I CCWWGG 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 605
ErhI CCWWGG 1 cut(s) 538
FaeI CATG 3 cut(s) 238, 497, 674
FaiI YATR 6 cut(s) 47, 236, 302, 495, 567, 672
FaqI GGGAC 2 cut(s) 221, 244
FatI CATG 3 cut(s) 234, 493, 670
FbaI TGATCA 1 cut(s) 465
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 5 cut(s) 40, 117, 173, 176, 681
FriOI GRGCYC 1 cut(s) 605
Fsp4HI GCNGC 5 cut(s) 40, 117, 173, 176, 681
GlaI GCGC 1 cut(s) 179
GluI GCNGC 5 cut(s) 40, 117, 173, 176, 681
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 2 cut(s) 656, 719
HapII CCGG 3 cut(s) 191, 346, 560
HgaI GACGC 3 cut(s) 196, 304, 686
HhaI GCGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 3 cut(s) 238, 497, 674
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 4 cut(s) 489, 497, 506, 512
HpaII CCGG 3 cut(s) 191, 346, 560
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 3 cut(s) 6, 112, 268
Hpy188I TCNGA 3 cut(s) 417, 511, 593
Hpy188III TCNNGA 4 cut(s) 218, 400, 445, 469
Hpy8I GTNNAC 3 cut(s) 6, 112, 268
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 6 cut(s) 49, 94, 197, 244, 592, 637
HpyCH4IV ACGT 2 cut(s) 544, 571
HpyCH4V TGCA 9 cut(s) 39, 69, 172, 213, 238, 381, 548, 554, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 330, 689
HpyF3I CTNAG 3 cut(s) 12, 77, 688
HpySE526I ACGT 2 cut(s) 544, 571
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 3 cut(s) 238, 497, 674
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 465
Kzo9I GATC 4 cut(s) 73, 366, 373, 465
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 3 cut(s) 47, 608, 697
Lsp1109I GCAGC 5 cut(s) 51, 103, 184, 187, 667
MabI ACCWGGT 1 cut(s) 408
MaeII ACGT 2 cut(s) 544, 571
MaeIII GTNAC 1 cut(s) 308
MalI GATC 4 cut(s) 75, 368, 375, 467
MboI GATC 4 cut(s) 73, 366, 373, 465
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 2 cut(s) 27, 605
MluCI AATT 5 cut(s) 88, 239, 293, 616, 641
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 2 cut(s) 526, 646
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 3 cut(s) 191, 346, 560
MspR9I CCNGG 6 cut(s) 263, 347, 410, 503, 560, 561
MunI CAATTG 1 cut(s) 641
MvaI CCWGG 3 cut(s) 263, 410, 503
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 321, 330, 689
NciI CCSGG 3 cut(s) 347, 560, 561
NdeII GATC 4 cut(s) 73, 366, 373, 465
NlaIII CATG 3 cut(s) 238, 497, 674
NlaIV GGNNCC 4 cut(s) 26, 227, 351, 604
NmuCI GTSAC 1 cut(s) 308
PciSI GCTCTTC 1 cut(s) 323
PfeI GAWTC 4 cut(s) 489, 497, 506, 512
PflFI GACNNNGTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 714
PkrI GCNGC 5 cut(s) 41, 118, 174, 177, 682
PpuMI RGGWCCY 1 cut(s) 726
Psp5II RGGWCCY 1 cut(s) 726
Psp6I CCWGG 3 cut(s) 261, 408, 501
PspGI CCWGG 3 cut(s) 261, 408, 501
PspN4I GGNNCC 4 cut(s) 26, 227, 351, 604
PspPI GGNCC 2 cut(s) 717, 726
PspPPI RGGWCCY 1 cut(s) 726
PsrI GAACNNNNNNTAC 2 cut(s) 440, 472
PstI CTGCAG 3 cut(s) 41, 174, 725
PstNI CAGNNNCTG 1 cut(s) 714
PsuI RGATCY 1 cut(s) 73
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
SapI GCTCTTC 1 cut(s) 323
SatI GCNGC 5 cut(s) 40, 117, 173, 176, 681
Sau3AI GATC 4 cut(s) 73, 366, 373, 465
Sau96I GGNCC 2 cut(s) 717, 726
SbfI CCTGCAGG 1 cut(s) 725
ScrFI CCNGG 6 cut(s) 263, 347, 410, 503, 560, 561
SdaI CCTGCAGG 1 cut(s) 725
SduI GDGCHC 2 cut(s) 27, 605
SexAI ACCWGGT 1 cut(s) 408
SfcI CTRYAG 3 cut(s) 37, 170, 721
SinI GGWCC 1 cut(s) 726
SmaI CCCGGG 1 cut(s) 561
Sse8387I CCTGCAGG 1 cut(s) 725
Sse9I AATT 5 cut(s) 88, 239, 293, 616, 641
SsiI CCGC 3 cut(s) 359, 389, 705
StyD4I CCNGG 6 cut(s) 261, 345, 408, 501, 558, 559
StyI CCWWGG 1 cut(s) 538
TaiI ACGT 2 cut(s) 547, 574
TaqI TCGA 3 cut(s) 243, 297, 369
TasI AATT 5 cut(s) 88, 239, 293, 616, 641
TfiI GAWTC 4 cut(s) 489, 497, 506, 512
TscAI CASTG 2 cut(s) 85, 383
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 5 cut(s) 39, 116, 172, 175, 680
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 2 cut(s) 399, 687
TspGWI ACGGA 1 cut(s) 97
TspMI CCCGGG 1 cut(s) 559
TspRI CASTG 2 cut(s) 85, 383
Tth111I GACNNNGTC 1 cut(s) 314
Van91I CCANNNNNTGG 1 cut(s) 714
VpaK11BI GGWCC 1 cut(s) 726
XapI RAATTY 1 cut(s) 293
XmaI CCCGGG 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.