Rh4BG288400

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004072
Physical Location & Seq
Reverse (-)
46125728 .. 46126648
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG288400.1

Sequence Viewer

Length: 921 bp
ATGGATTGCAGTGCTATTGAACTGAAGGTTATCTCCTGTAAGGATCTCAAAGCCTTCAATTTCTTCCAGAAGCTTTCAGTCTATGCAGACGTCTCCATCTTCAATGATGAAATCAAGAAAGAGCAGCAACAGCAAGAACATCTGCTGCAGCGCCAGAAAACACCAGTCGACAGAGATGGAGATGGGAACCCGGAGTGGAATCATACGATGAGCTTCGATACGAAAGCCATGTCATCGCTCGATGATCAATGGGATCACTGGTATATCAAGTTCGACATGCGCAATGATGGTGCAGTTTTGGGGAAGAGCCTTGGCAAAGTGCAGGTACCTTTGAAAGAGTTGATCGAGGAGTTCAATGGAGCAGTCAGGTTTATGAGCTATCAAGTCAAGACTGGTGATGGGAAGTCTAATGGGGTCTTAGATTTTTCTTACCATGTAATTGGGAAGAAGAGCACCAACAACAATGGAATTCCTGATTCCGTAGTATCCGAAAAAACCAATGTTCCTGATCCTGCTGGTAAAGTCCAGGTTCAATCTCTTTACCCCACTTTAGAAGTAGAATCCCTGTCCCGGGATCTGTTCTATCCTTCATTAGCTGAGGTTTCCAGTCCCGTACCAAAAATTCCAGTCCCTTCTCCAAAGCTTAGCACTCAATTTTCGCTTCCTTCTCATCATCATCATCATCATCATCATCATCCTCCATGTCAAGCATTCTCGCCATTTGTTCAACCTCCGAGGATGTACTGGCATCCATCAGAGTTCACAAATCATGGTTGCAATTTCTGTAGCAACCCTGCCGGGTTAGGACAAGTTGGGATGGTGGATTATAAGGGTCCATGGAGGACTAATTTTCCAGGGAATAGAAGTGATCTTCTTCAAGCTCCAGATCCGGAAACTACTACCAATGGCAGATGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

306

Amino Acids

34.53

Weight (kDa)

6.3

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 114 3.4e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AatII GACGTC 1 cut(s) 93
Acc16I TGCGCA 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 313
Acc65I GGTACC 1 cut(s) 325
AccB1I GGYRCC 1 cut(s) 325
AccI GTMKAC 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 889
AclWI GGATC 5 cut(s) 51, 261, 503, 582, 881
AcsI RAATTY 2 cut(s) 468, 621
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 327, 615, 743
AfiI CCNNNNNNNGG 2 cut(s) 570, 571
AgsI TTSAA 8 cut(s) 20, 58, 103, 334, 355, 533, 728, 878
AjnI CCWGG 2 cut(s) 525, 853
AluBI AGCT 6 cut(s) 73, 213, 378, 596, 643, 881
AluI AGCT 6 cut(s) 73, 213, 378, 596, 643, 881
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 5 cut(s) 51, 261, 503, 582, 881
Ama87I CYCGRG 1 cut(s) 570
Aor13HI TCCGGA 1 cut(s) 889
ApeKI GCWGC 3 cut(s) 124, 145, 148
ApoI RAATTY 2 cut(s) 468, 621
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
Asp718I GGTACC 1 cut(s) 325
AspLEI GCGC 2 cut(s) 153, 282
AspS9I GGNCC 1 cut(s) 833
AsuC2I CCSGG 4 cut(s) 191, 571, 572, 799
AsuHPI GGTGA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 570
AvaII GGWCC 1 cut(s) 833
BanI GGYRCC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 455
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 132, 136, 160
BccI CCATC 7 cut(s) 104, 170, 176, 281, 392, 760, 811
BciT130I CCWGG 2 cut(s) 527, 855
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 4 cut(s) 191, 571, 572, 799
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 2 cut(s) 146, 784
BfoI RGCGCY 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 313
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 3 cut(s) 125, 146, 149
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 3 cut(s) 126, 147, 150
Bme1390I CCNGG 6 cut(s) 191, 527, 571, 572, 799, 855
Bme18I GGWCC 1 cut(s) 833
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 1 cut(s) 833
BmiI GGNNCC 3 cut(s) 188, 327, 834
BmrFI CCNGG 6 cut(s) 191, 527, 571, 572, 799, 855
BmsI GCATC 2 cut(s) 757, 902
BpmI CTGGAG 1 cut(s) 867
Bpu10I CCTNAGC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 644
BpuMI CCSGG 4 cut(s) 191, 571, 572, 799
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 310, 570, 734, 836, 854
BsaWI WCCGGW 1 cut(s) 889
Bsc4I CCNNNNNNNGG 2 cut(s) 570, 571
Bse1I ACTGG 6 cut(s) 164, 263, 397, 606, 626, 749
Bse3DI GCAATG 1 cut(s) 289
BseAI TCCGGA 1 cut(s) 889
BseBI CCWGG 2 cut(s) 527, 855
BseDI CCNNGG 5 cut(s) 310, 570, 734, 836, 854
BseGI GGATG 4 cut(s) 694, 744, 748, 822
BseLI CCNNNNNNNGG 2 cut(s) 570, 571
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 6 cut(s) 164, 263, 397, 606, 626, 749
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 3 cut(s) 132, 136, 160
BsgI GTGCAG 2 cut(s) 312, 341
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 455
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 4 cut(s) 191, 571, 798, 890
BslFI GGGAC 3 cut(s) 553, 594, 614
BslI CCNNNNNNNGG 2 cut(s) 570, 571
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 3 cut(s) 553, 594, 614
BsmI GAATGC 1 cut(s) 710
BsoBI CYCGRG 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 889
Bsp143I GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
Bsp1720I GCTNAGC 1 cut(s) 644
Bsp19I CCATGG 1 cut(s) 836
BspCNI CTCAG 1 cut(s) 589
BspEI TCCGGA 1 cut(s) 889
BspLI GGNNCC 3 cut(s) 188, 327, 834
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 5 cut(s) 51, 261, 503, 582, 881
BspQI GCTCTTC 2 cut(s) 299, 443
BspT107I GGYRCC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 289
BsrI ACTGG 6 cut(s) 164, 263, 397, 606, 626, 749
BssECI CCNNGG 5 cut(s) 310, 570, 734, 836, 854
BssMI GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 2 cut(s) 310, 836
Bst2UI CCWGG 2 cut(s) 527, 855
Bst6I CTCTTC 2 cut(s) 299, 443
BstACI GRCGYC 1 cut(s) 90
BstDEI CTNAG 3 cut(s) 418, 597, 644
BstDSI CCRYGG 1 cut(s) 836
BstF5I GGATG 4 cut(s) 694, 744, 748, 822
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 2 cut(s) 153, 282
BstKTI GATC 8 cut(s) 46, 247, 256, 345, 511, 577, 871, 889
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 2 cut(s) 527, 855
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 6 cut(s) 189, 525, 569, 570, 797, 853
BstSFI CTRYAG 2 cut(s) 146, 784
BstV1I GCAGC 3 cut(s) 132, 136, 160
BstX2I RGATCY 3 cut(s) 43, 574, 886
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 3 cut(s) 43, 574, 886
BsuI GTATCC 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 836
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 4 cut(s) 694, 744, 748, 822
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 2 cut(s) 16, 256
BveI ACCTGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 153, 282
Cfr13I GGNCC 1 cut(s) 833
Cfr9I CCCGGG 1 cut(s) 570
Csp6I GTAC 3 cut(s) 326, 614, 742
CviAII CATG 6 cut(s) 229, 277, 434, 702, 770, 837
CviJI RGCY 9 cut(s) 53, 73, 213, 227, 309, 378, 596, 643, 881
CviKI_1 RGCY 9 cut(s) 53, 73, 213, 227, 309, 378, 596, 643, 881
CviQI GTAC 3 cut(s) 326, 614, 742
DdeI CTNAG 3 cut(s) 418, 597, 644
DpnI GATC 8 cut(s) 45, 246, 255, 344, 510, 576, 870, 888
DpnII GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
Eam1104I CTCTTC 2 cut(s) 299, 443
EarI CTCTTC 2 cut(s) 299, 443
Eco130I CCWWGG 2 cut(s) 310, 836
Eco47I GGWCC 1 cut(s) 833
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 570
EcoRI GAATTC 1 cut(s) 468
EcoRII CCWGG 2 cut(s) 525, 853
EcoT14I CCWWGG 2 cut(s) 310, 836
ErhI CCWWGG 2 cut(s) 310, 836
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 6 cut(s) 232, 280, 437, 705, 773, 840
FaqI GGGAC 3 cut(s) 553, 594, 614
FatI CATG 6 cut(s) 228, 276, 433, 701, 769, 836
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 125, 146, 149
FokI GGATG 4 cut(s) 681, 735, 751, 829
Fsp4HI GCNGC 3 cut(s) 125, 146, 149
FspI TGCGCA 1 cut(s) 281
GlaI GCGC 2 cut(s) 152, 281
GluI GCNGC 3 cut(s) 125, 146, 149
GsuI CTGGAG 1 cut(s) 867
HaeII RGCGCY 1 cut(s) 154
HapII CCGG 4 cut(s) 191, 571, 798, 890
HhaI GCGC 2 cut(s) 153, 282
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 6 cut(s) 232, 280, 437, 705, 773, 840
Hin6I GCGC 2 cut(s) 151, 280
HinP1I GCGC 2 cut(s) 151, 280
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 71, 641
HinfI GANTC 3 cut(s) 199, 476, 560
HpaII CCGG 4 cut(s) 191, 571, 798, 890
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 169, 762
Hpy188I TCNGA 3 cut(s) 490, 735, 757
Hpy188III TCNNGA 7 cut(s) 67, 115, 388, 473, 506, 884, 890
Hpy8I GTNNAC 2 cut(s) 169, 762
HpyAV CCTTC 5 cut(s) 19, 64, 597, 642, 675
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 6 cut(s) 9, 86, 148, 293, 322, 777
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 3 cut(s) 418, 597, 644
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 6 cut(s) 232, 280, 437, 705, 773, 840
HspAI GCGC 2 cut(s) 151, 280
Kpn2I TCCGGA 1 cut(s) 889
KpnI GGTACC 1 cut(s) 329
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
LguI GCTCTTC 2 cut(s) 299, 443
LmnI GCTCC 2 cut(s) 359, 886
Lsp1109I GCAGC 3 cut(s) 132, 136, 160
LweI GCATC 2 cut(s) 757, 902
MaeII ACGT 1 cut(s) 90
MalI GATC 8 cut(s) 45, 246, 255, 344, 510, 576, 870, 888
MboI GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
MboII GAAGA 7 cut(s) 55, 91, 316, 457, 460, 863, 866
MflI RGATCY 3 cut(s) 43, 574, 886
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 7 cut(s) 58, 438, 468, 621, 653, 778, 847
MnlI CCTC 6 cut(s) 340, 592, 708, 729, 741, 834
MroI TCCGGA 1 cut(s) 889
MspI CCGG 4 cut(s) 191, 571, 798, 890
MspR9I CCNGG 6 cut(s) 191, 527, 571, 572, 799, 855
Mva1269I GAATGC 1 cut(s) 710
MvaI CCWGG 2 cut(s) 527, 855
MwoI GCNNNNNNNGC 1 cut(s) 130
NciI CCSGG 4 cut(s) 191, 571, 572, 799
NcoI CCATGG 1 cut(s) 836
NdeII GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
NlaIII CATG 6 cut(s) 232, 280, 437, 705, 773, 840
NlaIV GGNNCC 3 cut(s) 188, 327, 834
NsbI TGCGCA 1 cut(s) 281
NspI RCATGY 1 cut(s) 280
PciSI GCTCTTC 2 cut(s) 299, 443
PctI GAATGC 1 cut(s) 710
PfeI GAWTC 3 cut(s) 199, 476, 560
PkrI GCNGC 3 cut(s) 126, 147, 150
PsiI TTATAA 1 cut(s) 828
Psp6I CCWGG 2 cut(s) 525, 853
PspGI CCWGG 2 cut(s) 525, 853
PspN4I GGNNCC 3 cut(s) 188, 327, 834
PspPI GGNCC 1 cut(s) 833
PsrI GAACNNNNNNTAC 2 cut(s) 254, 286
PstI CTGCAG 1 cut(s) 150
PsuI RGATCY 3 cut(s) 43, 574, 886
RsaI GTAC 3 cut(s) 327, 615, 743
RsaNI GTAC 3 cut(s) 326, 614, 742
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 2 cut(s) 299, 443
SatI GCNGC 3 cut(s) 125, 146, 149
Sau3AI GATC 8 cut(s) 43, 244, 253, 342, 508, 574, 868, 886
Sau96I GGNCC 1 cut(s) 833
ScrFI CCNGG 6 cut(s) 191, 527, 571, 572, 799, 855
SduI GDGCHC 1 cut(s) 455
SfaNI GCATC 2 cut(s) 757, 902
SfcI CTRYAG 2 cut(s) 146, 784
SinI GGWCC 1 cut(s) 833
SmaI CCCGGG 1 cut(s) 572
Sse9I AATT 7 cut(s) 58, 438, 468, 621, 653, 778, 847
StyD4I CCNGG 6 cut(s) 189, 525, 569, 570, 797, 853
StyI CCWWGG 2 cut(s) 310, 836
TaiI ACGT 1 cut(s) 93
TaqI TCGA 5 cut(s) 168, 216, 240, 273, 345
TasI AATT 7 cut(s) 58, 438, 468, 621, 653, 778, 847
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 3 cut(s) 199, 476, 560
TscAI CASTG 2 cut(s) 16, 263
TseI GCWGC 3 cut(s) 124, 145, 148
TspDTI ATGAA 2 cut(s) 123, 579
TspGWI ACGGA 1 cut(s) 469
TspMI CCCGGG 1 cut(s) 570
TspRI CASTG 2 cut(s) 16, 263
VpaK11BI GGWCC 1 cut(s) 833
XapI RAATTY 2 cut(s) 468, 621
XceI RCATGY 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 570
XmiI GTMKAC 1 cut(s) 168
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.