FvH4_4g22200

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004072
Physical Location & Seq
Reverse (-)
25100881 .. 25101792
912 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22200.t1

Sequence Viewer

Length: 912 bp
ATGGATTGCAGTGCTCTAGAACTGAAGGTTATATCCTGTCAGGATCTCAAAGCCTTCAATTTCTTCCAGAAGCTTTCCGTCTATGCAGACGTCTCCATCTTCAATGAAGACTTGAAGAAAGAGCAGCAACAGCAAGAACATCTACAGCAGCGCCAGAAAACGCCAGTCGACAGAGATGGAGATGGTAACCCGGAGTGGAATCACATGATGAGCTTCGATATGAAAGCCATGTCAGCTCTTGATGATCAATGGAATCACTGGTTTATCAAGTTCGACATGCGCAATGATGGTGCAGTTCTGGGGAAGAGCCTTGGCAAAGTGCAGGTACGGTTTAAGGAGTTGATTGAGGAGTTCAATGGAGCAGTCAGGTTTATGAGTTATCAAGTGAAGACAAGTGATGGTAAGTCTAACGGGGTCTTGGATTTTTCATACCAGGTAGTTAGGAAGAACACCAGCAATGGAATTCCTGATTCCGTTGTATCTGAAAAAACCAATGTTGTTGATCCTACTGATAAAGTTCAGGTTCAACCTCTTTACCCTACTTTAGAAGTAGAATCCCTGTCCCGTGATCTGTTCTATCCTTCACTAGTTGAGGTCTCCAGTCCTGTACCAAAAATTCCAATCCCTTCTCCAATGCTCAACACTCAGTTTTCGCTTCCTCCTCATCATCATCGTCATCATCATCATCATCACCATCAAGCATTCTCGCCATTTGTTCAACCTCCGACTATGTACTGGCATCCATCAGAGTTCACAAATCATGGCTGCAATTTCTGTGGCAACCCTGCAGGGTTAGGACAAGTTGGGATGGTGGATTATAAGGGTCCGTGGAGGACTAATTGTCCAGGGAATAGAAGTGGTCTTCTTCAAGCTCCAGATCCGGAAACAACTACCAATGGCAGATGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

304

Amino Acids

34.52

Weight (kDa)

6.3

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 113 1.3e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 819
AatII GACGTC 1 cut(s) 93
Acc16I TGCGCA 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 313
AccI GTMKAC 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 880
AclWI GGATC 3 cut(s) 51, 497, 872
AcsI RAATTY 2 cut(s) 462, 615
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 327, 609, 734
AgsI TTSAA 7 cut(s) 58, 103, 115, 355, 527, 719, 869
AhdI GACNNNNNGTC 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 2 cut(s) 432, 844
AluBI AGCT 4 cut(s) 73, 213, 236, 872
AluI AGCT 4 cut(s) 73, 213, 236, 872
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 2 cut(s) 97, 601
AlwI GGATC 3 cut(s) 51, 497, 872
Aor13HI TCCGGA 1 cut(s) 880
ApeKI GCWGC 3 cut(s) 124, 148, 765
ApoI RAATTY 2 cut(s) 462, 615
AspLEI GCGC 2 cut(s) 153, 282
AspS9I GGNCC 1 cut(s) 824
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 683
AvaII GGWCC 1 cut(s) 824
BbsI GAAGAC 3 cut(s) 114, 395, 854
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 136, 160, 752
BccI CCATC 8 cut(s) 104, 170, 176, 281, 392, 702, 751, 802
BciT130I CCWGG 2 cut(s) 434, 846
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 2 cut(s) 97, 601
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 2 cut(s) 17, 587
BfmI CTRYAG 2 cut(s) 143, 786
BfoI RGCGCY 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 3 cut(s) 125, 149, 766
BlsI GCNGC 3 cut(s) 126, 150, 767
Bme1390I CCNGG 3 cut(s) 191, 434, 846
Bme18I GGWCC 1 cut(s) 824
BmeRI GACNNNNNGTC 1 cut(s) 840
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 1 cut(s) 825
BmrFI CCNGG 3 cut(s) 191, 434, 846
BmsI GCATC 2 cut(s) 748, 893
BpiI GAAGAC 3 cut(s) 114, 395, 854
BpmI CTGGAG 2 cut(s) 583, 858
BpuMI CCSGG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 3 cut(s) 310, 827, 845
BsaWI WCCGGW 1 cut(s) 880
Bse1I ACTGG 4 cut(s) 164, 263, 600, 740
Bse3DI GCAATG 2 cut(s) 289, 463
BseAI TCCGGA 1 cut(s) 880
BseBI CCWGG 2 cut(s) 434, 846
BseDI CCNNGG 3 cut(s) 310, 827, 845
BseGI GGATG 2 cut(s) 739, 813
BseMI GCAATG 2 cut(s) 289, 463
BseMII CTCAG 1 cut(s) 659
BseNI ACTGG 4 cut(s) 164, 263, 600, 740
BseRI GAGGAG 2 cut(s) 362, 651
BseXI GCAGC 3 cut(s) 136, 160, 752
BsgI GTGCAG 2 cut(s) 312, 341
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 2 cut(s) 191, 881
BslFI GGGAC 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 97, 601
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 547
BsmI GAATGC 1 cut(s) 701
Bso31I GGTCTC 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 16
Bsp13I TCCGGA 1 cut(s) 880
Bsp143I GATC 5 cut(s) 43, 244, 502, 568, 877
BspCNI CTCAG 1 cut(s) 658
BspEI TCCGGA 1 cut(s) 880
BspLI GGNNCC 1 cut(s) 825
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 3 cut(s) 51, 497, 872
BspQI GCTCTTC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 601
BsrDI GCAATG 2 cut(s) 289, 463
BsrI ACTGG 4 cut(s) 164, 263, 600, 740
BssECI CCNNGG 3 cut(s) 310, 827, 845
BssMI GATC 5 cut(s) 43, 244, 502, 568, 877
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 2 cut(s) 434, 846
Bst4CI ACNGT 1 cut(s) 330
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 645
BstDSI CCRYGG 1 cut(s) 827
BstEII GGTNACC 1 cut(s) 185
BstF5I GGATG 2 cut(s) 739, 813
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 2 cut(s) 153, 282
BstKTI GATC 5 cut(s) 46, 247, 505, 571, 880
BstMAI GTCTC 2 cut(s) 97, 601
BstMBI GATC 5 cut(s) 43, 244, 502, 568, 877
BstMWI GCNNNNNNNGC 2 cut(s) 130, 233
BstNI CCWGG 2 cut(s) 434, 846
BstNSI RCATGY 1 cut(s) 280
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 189, 432, 844
BstSFI CTRYAG 2 cut(s) 143, 786
BstV1I GCAGC 3 cut(s) 136, 160, 752
BstV2I GAAGAC 3 cut(s) 114, 395, 854
BstX2I RGATCY 2 cut(s) 43, 877
BstYI RGATCY 2 cut(s) 43, 877
BtgI CCRYGG 1 cut(s) 827
BtsCI GGATG 2 cut(s) 739, 813
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 2 cut(s) 16, 256
BveI ACCTGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 153, 282
Cfr13I GGNCC 1 cut(s) 824
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 3 cut(s) 326, 608, 733
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 4 cut(s) 205, 229, 277, 761
CviJI RGCY 8 cut(s) 53, 73, 213, 227, 236, 309, 765, 872
CviKI_1 RGCY 8 cut(s) 53, 73, 213, 227, 236, 309, 765, 872
CviQI GTAC 3 cut(s) 326, 608, 733
DdeI CTNAG 1 cut(s) 645
DpnI GATC 5 cut(s) 45, 246, 504, 570, 879
DpnII GATC 5 cut(s) 43, 244, 502, 568, 877
DriI GACNNNNNGTC 1 cut(s) 840
Eam1104I CTCTTC 1 cut(s) 299
Eam1105I GACNNNNNGTC 1 cut(s) 840
EarI CTCTTC 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 824
Eco57I CTGAAG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 185
EcoO65I GGTNACC 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 432, 844
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 4 cut(s) 208, 232, 280, 764
FaqI GGGAC 1 cut(s) 547
FatI CATG 4 cut(s) 204, 228, 276, 760
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 125, 149, 766
FokI GGATG 2 cut(s) 726, 820
Fsp4HI GCNGC 3 cut(s) 125, 149, 766
FspBI CTAG 2 cut(s) 17, 587
FspI TGCGCA 1 cut(s) 281
GlaI GCGC 2 cut(s) 152, 281
GluI GCNGC 3 cut(s) 125, 149, 766
GsuI CTGGAG 2 cut(s) 583, 858
HaeII RGCGCY 1 cut(s) 154
HapII CCGG 2 cut(s) 191, 881
HhaI GCGC 2 cut(s) 153, 282
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 4 cut(s) 208, 232, 280, 764
Hin6I GCGC 2 cut(s) 151, 280
HinP1I GCGC 2 cut(s) 151, 280
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 199, 253, 470, 554
HpaII CCGG 2 cut(s) 191, 881
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 2 cut(s) 169, 753
Hpy188I TCNGA 3 cut(s) 484, 726, 748
Hpy188III TCNNGA 7 cut(s) 17, 41, 67, 239, 467, 875, 881
Hpy8I GTNNAC 2 cut(s) 169, 753
HpyAV CCTTC 4 cut(s) 19, 64, 591, 636
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 6 cut(s) 9, 86, 293, 322, 768, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 233
HpyF3I CTNAG 1 cut(s) 645
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 4 cut(s) 208, 232, 280, 764
HspAI GCGC 2 cut(s) 151, 280
Kpn2I TCCGGA 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 5 cut(s) 43, 244, 502, 568, 877
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 2 cut(s) 359, 877
Lsp1109I GCAGC 3 cut(s) 136, 160, 752
LweI GCATC 2 cut(s) 748, 893
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 2 cut(s) 17, 587
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 185
MalI GATC 5 cut(s) 45, 246, 504, 570, 879
MboI GATC 5 cut(s) 43, 244, 502, 568, 877
MboII GAAGA 9 cut(s) 55, 91, 119, 127, 316, 400, 457, 854, 857
MflI RGATCY 2 cut(s) 43, 877
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 5 cut(s) 58, 462, 615, 769, 838
MmeI TCCRAC 1 cut(s) 749
MnlI CCTC 7 cut(s) 340, 540, 586, 669, 672, 732, 825
MroI TCCGGA 1 cut(s) 880
MseI TTAA 1 cut(s) 333
MspI CCGG 2 cut(s) 191, 881
MspR9I CCNGG 3 cut(s) 191, 434, 846
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 2 cut(s) 434, 846
MwoI GCNNNNNNNGC 2 cut(s) 130, 233
NciI CCSGG 1 cut(s) 191
NdeII GATC 5 cut(s) 43, 244, 502, 568, 877
NlaIII CATG 4 cut(s) 208, 232, 280, 764
NlaIV GGNNCC 1 cut(s) 825
NsbI TGCGCA 1 cut(s) 281
NspI RCATGY 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 299
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 4 cut(s) 199, 253, 470, 554
PkrI GCNGC 3 cut(s) 126, 150, 767
PsiI TTATAA 1 cut(s) 819
Psp6I CCWGG 2 cut(s) 432, 844
PspEI GGTNACC 1 cut(s) 185
PspGI CCWGG 2 cut(s) 432, 844
PspN4I GGNNCC 1 cut(s) 825
PspPI GGNCC 1 cut(s) 824
PstI CTGCAG 1 cut(s) 790
PsuI RGATCY 2 cut(s) 43, 877
RsaI GTAC 3 cut(s) 327, 609, 734
RsaNI GTAC 3 cut(s) 326, 608, 733
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 3 cut(s) 125, 149, 766
Sau3AI GATC 5 cut(s) 43, 244, 502, 568, 877
Sau96I GGNCC 1 cut(s) 824
SbfI CCTGCAGG 1 cut(s) 790
ScrFI CCNGG 3 cut(s) 191, 434, 846
SdaI CCTGCAGG 1 cut(s) 790
SduI GDGCHC 1 cut(s) 16
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 2 cut(s) 748, 893
SfcI CTRYAG 2 cut(s) 143, 786
SinI GGWCC 1 cut(s) 824
SpeI ACTAGT 1 cut(s) 586
Sse8387I CCTGCAGG 1 cut(s) 790
Sse9I AATT 5 cut(s) 58, 462, 615, 769, 838
SspMI CTAG 2 cut(s) 17, 587
StyD4I CCNGG 3 cut(s) 189, 432, 844
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 330
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 168, 216, 273
TasI AATT 5 cut(s) 58, 462, 615, 769, 838
TatI WGTACW 1 cut(s) 732
TfiI GAWTC 4 cut(s) 199, 253, 470, 554
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 2 cut(s) 16, 263
TseI GCWGC 3 cut(s) 124, 148, 765
TspDTI ATGAA 3 cut(s) 120, 236, 417
TspGWI ACGGA 3 cut(s) 67, 463, 816
TspRI CASTG 2 cut(s) 16, 263
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 2 cut(s) 462, 615
XbaI TCTAGA 1 cut(s) 16
XceI RCATGY 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 2 cut(s) 17, 587
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.