MD16G1144500.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004072
Physical Location & Seq
Forward (+)
11109377 .. 11110255
879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1144500.v1.1.491

Sequence Viewer

Length: 879 bp
ATGTCCACAGCTGAGAAAATCAGGCACCTGAAAGATTGCAGCTCCATAGAACTGAAGGTAATCTCCTGCAAGGATCTCAGTGCCTTCAATTTCTTCAACAGGCTGTCTGTCTACGCTGCTGTCTCCATCTTCAACGACGAAGCGAAGAAAGAAGAACAGAAGAAACATCTGCAGCAACGCCAGAAGACGCCGGTTGATAGAGAAGGGGACTGCAATCCTGAATGGAACCACCCCATGCAATTCGACACGAAGGACTTGTCCCTGGTTGACGAGTTCGATAATCTCTTTGTAGAATTCAACATACGATGTGACAGCGTCTTTGGCAAGAAGAGCATTGGAAAAGTCCGGGTGCCATTCGCGGACTTGATCGATGATCAGTGCAATGAAGCAGTCAGGTTCGTGAGCTACCAGGTTCGGACAAGCGATGGTAAGCCTAATGGGGTCTTGAACTTTTCTTACAAGGTGATCAAGAAGAACACTAAAGTTGGAATCCGTGATTCCCAGGAATCCGATTCCTCGTCCAAATTAACCACAGCCAGTGTTTATCCTGATCCTGCTGATAATACAGTCCAATCTCTTTACCCCACCTTGGACGTGCAAGTGCAATCCCGGGATTTAAACGTATCCTATCCTTCGCTATCCGATGTTCGGGCTCCTGTGCCGACAATTTCAGTTCCTTCTCCCAAGTTCAATTGCCATTGGAGGCCAGAACCCTACAACATGAAGACGCTGCCATCTCAGCTCCCGTTTTCTCCACCGGCAACTGGGCCTGCAGGTGTTAATTGTCATCCATGTCAGCCCTTGCCATTTGCACAGTCTCCAACTCCAAGGCCATATTTTGGTAATTACAGTTGCTATGAGAATCCAGCCGGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

293

Amino Acids

32.81

Weight (kDa)

6.94

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 16 - 125 3.7e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 764
AccB1I GGYRCC 2 cut(s) 24, 349
AccB7I CCANNNNNTGG 1 cut(s) 839
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 359
AciI CCGC 1 cut(s) 359
AclWI GGATC 2 cut(s) 81, 545
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 188
AfiI CCNNNNNNNGG 4 cut(s) 589, 648, 764, 839
AgsI TTSAA 6 cut(s) 88, 97, 133, 298, 448, 691
AjiI CACGTC 1 cut(s) 595
AjnI CCWGG 3 cut(s) 261, 408, 501
AluBI AGCT 4 cut(s) 11, 42, 405, 742
AluI AGCT 4 cut(s) 11, 42, 405, 742
Alw26I GTCTC 2 cut(s) 127, 822
AlwI GGATC 2 cut(s) 81, 545
Ama87I CYCGRG 1 cut(s) 609
AoxI GGCC 3 cut(s) 704, 767, 830
ApeKI GCWGC 4 cut(s) 39, 116, 172, 730
ApoI RAATTY 1 cut(s) 293
ArsI GACNNNNNNTTYG 4 cut(s) 242, 274, 302, 334
AspS9I GGNCC 1 cut(s) 767
AsuC2I CCSGG 4 cut(s) 347, 610, 611, 871
AsuHPI GGTGA 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 609
BanI GGYRCC 2 cut(s) 24, 349
BanII GRGCYC 1 cut(s) 655
BbsI GAAGAC 2 cut(s) 191, 731
BbvI GCAGC 4 cut(s) 51, 103, 184, 717
BccI CCATC 3 cut(s) 134, 419, 742
BciT130I CCWGG 3 cut(s) 263, 410, 503
BciVI GTATCC 1 cut(s) 634
BclI TGATCA 2 cut(s) 373, 465
BcnI CCSGG 4 cut(s) 347, 610, 611, 871
BcoDI GTCTC 2 cut(s) 127, 822
BfmI CTRYAG 2 cut(s) 170, 771
BfuAI ACCTGC 1 cut(s) 764
BfuI GTATCC 1 cut(s) 634
BisI GCNGC 4 cut(s) 40, 117, 173, 731
BlsI GCNGC 4 cut(s) 41, 118, 174, 732
Bme1390I CCNGG 7 cut(s) 263, 347, 410, 503, 610, 611, 871
BmeT110I CYCGRG 1 cut(s) 609
BmgBI CACGTC 1 cut(s) 595
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 4 cut(s) 26, 227, 351, 654
BmrFI CCNGG 7 cut(s) 263, 347, 410, 503, 610, 611, 871
BmrI ACTGGG 1 cut(s) 774
BmuI ACTGGG 1 cut(s) 774
BpiI GAAGAC 2 cut(s) 191, 731
BpuMI CCSGG 4 cut(s) 347, 610, 611, 871
Bsa29I ATCGAT 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 188
BsaJI CCNNGG 5 cut(s) 261, 501, 588, 609, 827
Bsc4I CCNNNNNNNGG 4 cut(s) 589, 648, 764, 839
Bse118I RCCGGY 2 cut(s) 190, 757
Bse1I ACTGG 2 cut(s) 537, 769
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 3 cut(s) 263, 410, 503
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 5 cut(s) 261, 501, 588, 609, 827
BseGI GGATG 1 cut(s) 787
BseLI CCNNNNNNNGG 4 cut(s) 589, 648, 764, 839
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 91, 752
BseNI ACTGG 2 cut(s) 537, 769
BseXI GCAGC 4 cut(s) 51, 103, 184, 717
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 3 cut(s) 706, 769, 832
BshNI GGYRCC 2 cut(s) 24, 349
BshVI ATCGAT 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 5 cut(s) 191, 346, 610, 758, 870
BslFI GGGAC 2 cut(s) 221, 244
BslI CCNNNNNNNGG 4 cut(s) 589, 648, 764, 839
BsmAI GTCTC 2 cut(s) 127, 822
BsmFI GGGAC 2 cut(s) 221, 244
BsnI GGCC 3 cut(s) 706, 769, 832
BsoBI CYCGRG 1 cut(s) 609
Bsp1286I GDGCHC 1 cut(s) 655
Bsp143I GATC 5 cut(s) 73, 366, 373, 465, 550
BspACI CCGC 1 cut(s) 359
BspANI GGCC 3 cut(s) 706, 769, 832
BspCNI CTCAG 3 cut(s) 4, 90, 751
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 26, 227, 351, 654
BspMAI CTGCAG 2 cut(s) 174, 775
BspMI ACCTGC 1 cut(s) 764
BspPI GGATC 2 cut(s) 81, 545
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 24, 349
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 2 cut(s) 190, 757
BsrI ACTGG 2 cut(s) 537, 769
BssAI RCCGGY 2 cut(s) 190, 757
BssECI CCNNGG 5 cut(s) 261, 501, 588, 609, 827
BssMI GATC 5 cut(s) 73, 366, 373, 465, 550
BssNI GRCGYC 1 cut(s) 188
BssT1I CCWWGG 2 cut(s) 588, 827
Bst2UI CCWGG 3 cut(s) 263, 410, 503
Bst4CI ACNGT 3 cut(s) 568, 816, 851
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 771
BstDEI CTNAG 3 cut(s) 12, 77, 738
BstF5I GGATG 1 cut(s) 787
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 5 cut(s) 76, 369, 376, 468, 553
BstMAI GTCTC 2 cut(s) 127, 822
BstMBI GATC 5 cut(s) 73, 366, 373, 465, 550
BstMWI GCNNNNNNNGC 3 cut(s) 321, 330, 739
BstNI CCWGG 3 cut(s) 263, 410, 503
BstSCI CCNGG 7 cut(s) 261, 345, 408, 501, 608, 609, 869
BstSFI CTRYAG 2 cut(s) 170, 771
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 4 cut(s) 51, 103, 184, 717
BstV2I GAAGAC 2 cut(s) 191, 731
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 369
BsuI GTATCC 1 cut(s) 634
BsuRI GGCC 3 cut(s) 706, 769, 832
BsuTUI ATCGAT 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 438
BtrI CACGTC 1 cut(s) 595
BtsCI GGATG 1 cut(s) 787
BtsIMutI CAGTG 3 cut(s) 85, 383, 544
BveI ACCTGC 1 cut(s) 764
Cac8I GCNNGC 1 cut(s) 771
Cfr10I RCCGGY 2 cut(s) 190, 757
Cfr13I GGNCC 1 cut(s) 767
Cfr9I CCCGGG 1 cut(s) 609
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 3 cut(s) 196, 304, 736
CsiI ACCWGGT 1 cut(s) 408
CviAII CATG 3 cut(s) 235, 721, 792
DdeI CTNAG 3 cut(s) 12, 77, 738
DpnI GATC 5 cut(s) 75, 368, 375, 467, 552
DpnII GATC 5 cut(s) 73, 366, 373, 465, 550
DraI TTTAAA 1 cut(s) 618
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Eco130I CCWWGG 2 cut(s) 588, 827
Eco24I GRGCYC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 609
EcoRI GAATTC 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 261, 408, 501
EcoT14I CCWWGG 2 cut(s) 588, 827
EcoT38I GRGCYC 1 cut(s) 655
ErhI CCWWGG 2 cut(s) 588, 827
FaeI CATG 3 cut(s) 238, 724, 795
FaiI YATR 7 cut(s) 47, 236, 302, 722, 793, 835, 858
FaqI GGGAC 2 cut(s) 221, 244
FatI CATG 3 cut(s) 234, 720, 791
FbaI TGATCA 2 cut(s) 373, 465
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 4 cut(s) 40, 117, 173, 731
FokI GGATG 1 cut(s) 774
FriOI GRGCYC 1 cut(s) 655
Fsp4HI GCNGC 4 cut(s) 40, 117, 173, 731
GluI GCNGC 4 cut(s) 40, 117, 173, 731
HaeIII GGCC 3 cut(s) 706, 769, 832
HapII CCGG 5 cut(s) 191, 346, 610, 758, 870
HgaI GACGC 3 cut(s) 196, 304, 736
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 3 cut(s) 238, 724, 795
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 5 cut(s) 489, 497, 506, 512, 862
HpaII CCGG 5 cut(s) 191, 346, 610, 758, 870
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 3 cut(s) 6, 112, 268
Hpy188I TCNGA 3 cut(s) 417, 511, 643
Hpy188III TCNNGA 5 cut(s) 218, 400, 445, 469, 548
Hpy8I GTNNAC 3 cut(s) 6, 112, 268
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 6 cut(s) 49, 94, 197, 244, 642, 687
HpyCH4III ACNGT 3 cut(s) 568, 816, 851
HpyCH4IV ACGT 2 cut(s) 594, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 330, 739
HpyF3I CTNAG 3 cut(s) 12, 77, 738
HpySE526I ACGT 2 cut(s) 594, 621
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 3 cut(s) 238, 724, 795
Ksp22I TGATCA 2 cut(s) 373, 465
Kzo9I GATC 5 cut(s) 73, 366, 373, 465, 550
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 3 cut(s) 47, 658, 747
Lsp1109I GCAGC 4 cut(s) 51, 103, 184, 717
MabI ACCWGGT 1 cut(s) 408
MaeII ACGT 2 cut(s) 594, 621
MaeIII GTNAC 1 cut(s) 308
MalI GATC 5 cut(s) 75, 368, 375, 467, 552
MboI GATC 5 cut(s) 73, 366, 373, 465, 550
MboII GAAGA 9 cut(s) 85, 121, 157, 164, 172, 196, 340, 484, 736
MfeI CAATTG 1 cut(s) 691
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 655
MluCI AATT 8 cut(s) 88, 239, 293, 524, 666, 691, 781, 844
MmeI TCCRAC 2 cut(s) 466, 845
MnlI CCTC 2 cut(s) 526, 696
MseI TTAA 3 cut(s) 527, 617, 780
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 5 cut(s) 191, 346, 610, 758, 870
MspR9I CCNGG 7 cut(s) 263, 347, 410, 503, 610, 611, 871
MunI CAATTG 1 cut(s) 691
MvaI CCWGG 3 cut(s) 263, 410, 503
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 321, 330, 739
NciI CCSGG 4 cut(s) 347, 610, 611, 871
NdeII GATC 5 cut(s) 73, 366, 373, 465, 550
NlaIII CATG 3 cut(s) 238, 724, 795
NlaIV GGNNCC 4 cut(s) 26, 227, 351, 654
NmuCI GTSAC 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 764
PciSI GCTCTTC 1 cut(s) 323
PfeI GAWTC 5 cut(s) 489, 497, 506, 512, 862
PflFI GACNNNGTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 839
PkrI GCNGC 4 cut(s) 41, 118, 174, 732
Psp6I CCWGG 3 cut(s) 261, 408, 501
PspGI CCWGG 3 cut(s) 261, 408, 501
PspN4I GGNNCC 4 cut(s) 26, 227, 351, 654
PspPI GGNCC 1 cut(s) 767
PsrI GAACNNNNNNTAC 2 cut(s) 440, 472
PstI CTGCAG 2 cut(s) 174, 775
PsuI RGATCY 1 cut(s) 73
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 3 cut(s) 527, 617, 780
SatI GCNGC 4 cut(s) 40, 117, 173, 731
Sau3AI GATC 5 cut(s) 73, 366, 373, 465, 550
Sau96I GGNCC 1 cut(s) 767
SbfI CCTGCAGG 1 cut(s) 775
ScrFI CCNGG 7 cut(s) 263, 347, 410, 503, 610, 611, 871
SdaI CCTGCAGG 1 cut(s) 775
SduI GDGCHC 1 cut(s) 655
SexAI ACCWGGT 1 cut(s) 408
SfcI CTRYAG 2 cut(s) 170, 771
SmaI CCCGGG 1 cut(s) 611
Sse8387I CCTGCAGG 1 cut(s) 775
Sse9I AATT 8 cut(s) 88, 239, 293, 524, 666, 691, 781, 844
SsiI CCGC 1 cut(s) 359
StyD4I CCNGG 7 cut(s) 261, 345, 408, 501, 608, 609, 869
StyI CCWWGG 2 cut(s) 588, 827
TaaI ACNGT 3 cut(s) 568, 816, 851
TaiI ACGT 2 cut(s) 597, 624
TaqI TCGA 3 cut(s) 243, 276, 369
TasI AATT 8 cut(s) 88, 239, 293, 524, 666, 691, 781, 844
TfiI GAWTC 5 cut(s) 489, 497, 506, 512, 862
Tru1I TTAA 3 cut(s) 527, 617, 780
Tru9I TTAA 3 cut(s) 527, 617, 780
TscAI CASTG 3 cut(s) 85, 383, 544
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 4 cut(s) 39, 116, 172, 730
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 2 cut(s) 399, 737
TspGWI ACGGA 1 cut(s) 482
TspMI CCCGGG 1 cut(s) 609
TspRI CASTG 3 cut(s) 85, 383, 544
Tth111I GACNNNGTC 1 cut(s) 314
Van91I CCANNNNNTGG 1 cut(s) 839
XapI RAATTY 1 cut(s) 293
XmaI CCCGGG 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.