Rh4AG282800

Emopamil binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004072
Physical Location & Seq
Reverse (-)
59755593 .. 59757016
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG282800.1

Sequence Viewer

Length: 675 bp
ATGGAGGGATCTGACTCTTCTCTGAATCATCCGTACTCGCCTCAGAATGTAAAGCTACCGGGATTTGTACCTGTGGTTTATTCACAGGCCACCATTCTTGGCGTTTATGGCCTCGCTTCCCTACTCGTCGTTTCCCTTACTTGGCTCATCTCAGGGAGAGCCCCGAAGAAATCAAAACTTGATAGGTGGCTTATGTGTTGGTGGGCTTTCACGGGCCTCACGCATTTGATACTTGAAGGTTATTTTGCATTCTCGCCCGAGTTTTACAAGGATAAGACTGCTTGTTACCTGGCTGAAGTCTGGAAAGAATACAGCAAAGGTGATTCAAGATACGCATTTAGGGATGCAGGAGTTGTTGCTGTTGAAGGATTGACTGCAGTTATAGAGGGACCGGCTTGCCTTCTTGCAGTGTATGCAATAGCTAAAGGCAAGTCATATAGCTACGTGCTCCAGCTTGCCATTTCTTTGGGGCAGCTCTACGGAACTGCTGTATATTTCATTACGGCCTTCTTGGAAGGTGACCACTTTGCTGCAAGTCCACTATACTACTATGAATACTACATTGCTGCCAATGCTTCGTGGATCATTATACCCACACTCATTTCAATCCGGAGTTGGAAGAGGATTTGTGCAGCAGTACAGGCTCAAGGCCAGAAGAAGAACAAAACTCGCTGA

Protein Analysis

224

Amino Acids

24.95

Weight (kDa)

9.01

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 97 - 205 3.7e-23 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 609
AclWI GGATC 2 cut(s) 16, 590
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 3 cut(s) 35, 69, 639
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 4 cut(s) 236, 327, 365, 606
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 5 cut(s) 55, 422, 441, 454, 475
AluI AGCT 5 cut(s) 55, 422, 441, 454, 475
Alw21I GWGCWC 1 cut(s) 450
AlwI GGATC 2 cut(s) 16, 590
Ama87I CYCGRG 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 609
AoxI GGCC 5 cut(s) 87, 109, 214, 504, 649
ApeKI GCWGC 4 cut(s) 472, 530, 566, 632
AspS9I GGNCC 2 cut(s) 214, 389
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 332, 530
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 163
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 4 cut(s) 484, 517, 553, 644
BceAI ACGGC 1 cut(s) 519
BciT130I CCWGG 1 cut(s) 290
BcnI CCSGG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 4 cut(s) 473, 531, 567, 633
BlsI GCNGC 4 cut(s) 474, 532, 568, 634
Bme1390I CCNGG 2 cut(s) 60, 290
Bme18I GGWCC 1 cut(s) 389
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 214, 389
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 60, 290
BmsI GCATC 1 cut(s) 334
BpmI CTGGAG 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 630
BpuMI CCSGG 1 cut(s) 60
BsaAI YACGTR 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 609
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse118I RCCGGY 1 cut(s) 391
Bse3DI GCAATG 1 cut(s) 561
BseAI TCCGGA 1 cut(s) 609
BseBI CCWGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 28, 349
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 2 cut(s) 56, 165
BseXI GCAGC 4 cut(s) 484, 517, 553, 644
BsgI GTGCAG 1 cut(s) 651
BshFI GGCC 5 cut(s) 89, 111, 216, 506, 651
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 59, 392, 610
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 402
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 5 cut(s) 89, 111, 216, 506, 651
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 163, 450
Bsp13I TCCGGA 1 cut(s) 609
Bsp143I GATC 2 cut(s) 8, 582
BspANI GGCC 5 cut(s) 89, 111, 216, 506, 651
BspCNI CTCAG 2 cut(s) 55, 164
BspEI TCCGGA 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 390
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 2 cut(s) 16, 590
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 391
BssMI GATC 2 cut(s) 8, 582
Bst2UI CCWGG 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 22, 614
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 445
BstC8I GCNNGC 2 cut(s) 397, 456
BstDEI CTNAG 2 cut(s) 42, 151
BstEII GGTNACC 1 cut(s) 518
BstF5I GGATG 2 cut(s) 28, 349
BstKTI GATC 2 cut(s) 11, 585
BstMBI GATC 2 cut(s) 8, 582
BstMWI GCNNNNNNNGC 4 cut(s) 108, 413, 572, 641
BstNI CCWGG 1 cut(s) 290
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 58, 288
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 484, 517, 553, 644
BstX2I RGATCY 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 5 cut(s) 89, 111, 216, 506, 651
BtsCI GGATG 2 cut(s) 28, 349
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 397, 456
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 214, 389
Csp6I GTAC 3 cut(s) 34, 68, 638
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviQI GTAC 3 cut(s) 34, 68, 638
DdeI CTNAG 2 cut(s) 42, 151
DpnI GATC 2 cut(s) 10, 584
DpnII GATC 2 cut(s) 8, 582
Eam1104I CTCTTC 2 cut(s) 22, 614
EarI CTCTTC 2 cut(s) 22, 614
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 163
FaqI GGGAC 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 473, 531, 567, 633
FokI GGATG 2 cut(s) 15, 356
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 4 cut(s) 473, 531, 567, 633
GluI GCNGC 4 cut(s) 473, 531, 567, 633
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 5 cut(s) 89, 111, 216, 506, 651
HapII CCGG 3 cut(s) 59, 392, 610
HinfI GANTC 3 cut(s) 14, 25, 323
HpaII CCGG 3 cut(s) 59, 392, 610
HphI GGTGA 2 cut(s) 332, 530
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 3 cut(s) 13, 24, 45
Hpy188III TCNNGA 3 cut(s) 301, 327, 610
Hpy8I GTNNAC 1 cut(s) 539
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 5 cut(s) 230, 359, 410, 509, 517
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 7 cut(s) 248, 347, 377, 407, 416, 533, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 413, 572, 641
HpyF3I CTNAG 2 cut(s) 42, 151
HpySE526I ACGT 1 cut(s) 444
Kpn2I TCCGGA 1 cut(s) 609
Kzo9I GATC 2 cut(s) 8, 582
LmnI GCTCC 1 cut(s) 453
Lsp1109I GCAGC 4 cut(s) 484, 517, 553, 644
LweI GCATC 1 cut(s) 334
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 284, 518
MalI GATC 2 cut(s) 10, 584
MboI GATC 2 cut(s) 8, 582
MboII GAAGA 5 cut(s) 9, 178, 631, 667, 670
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 163, 450
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 596
MnlI CCTC 5 cut(s) 51, 122, 227, 379, 615
MroI TCCGGA 1 cut(s) 609
MspI CCGG 3 cut(s) 59, 392, 610
MspR9I CCNGG 2 cut(s) 60, 290
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 4 cut(s) 108, 413, 572, 641
NciI CCSGG 1 cut(s) 60
NdeII GATC 2 cut(s) 8, 582
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 518
PctI GAATGC 1 cut(s) 248
PfeI GAWTC 2 cut(s) 25, 323
PkrI GCNGC 4 cut(s) 474, 532, 568, 634
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 288
PspEI GGTNACC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 2 cut(s) 214, 389
PstI CTGCAG 1 cut(s) 379
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 3 cut(s) 35, 69, 639
RsaNI GTAC 3 cut(s) 34, 68, 638
SatI GCNGC 4 cut(s) 473, 531, 567, 633
Sau3AI GATC 2 cut(s) 8, 582
Sau96I GGNCC 2 cut(s) 214, 389
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 60, 290
SduI GDGCHC 2 cut(s) 163, 450
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 645
SmoI CTYRAG 1 cut(s) 645
StyD4I CCNGG 2 cut(s) 58, 288
TaiI ACGT 1 cut(s) 447
TatI WGTACW 1 cut(s) 637
TfiI GAWTC 2 cut(s) 25, 323
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 4 cut(s) 472, 530, 566, 632
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 2 cut(s) 487, 567
TspGWI ACGGA 2 cut(s) 21, 495
TspRI CASTG 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.