Rh4DG285500

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004072
Physical Location & Seq
Reverse (-)
50661681 .. 50662655
975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG285500.1

Sequence Viewer

Length: 975 bp
ATGTTTGAGCACCGGCTGAAACAAACTAGAATTTTGATGGATTGCAGTGCTATTGAACTGAAGGTTATCTCCTGTAAGGATCTCAAAGCCTTCAATTTCTTCCAGAAGCTTTCAGTCTATGCAGACGTCTCCATCTCCAATGATGAAATCAAGAAAGAGCAGCAACAGCAAGAACATCTGCAGCAGCGCCAGAAAACACCAGTCGACAGAGATGGAGATGGGAACCCGGAGTGGAATCATACGATGAGCTTCGAAACGAAAGCCATGTCATCGCTCGATGATCAATGGGATCACTGGTATATCAAGTTCGACATGCGCAATGATGGTGCAGTTTTGGGGAAGAGCCTTGGCAAAGTGCAGGTACCTTTGAAAGAGTTGATCGAGGAGTTCAATGGAGCAGTCAGGTTTATGAGGTATCAAGTCAAGACTGGTGATGGGAAGTCTAATGGGGTCTTAGATTTTTCTTACCATGTAATTGGGAAGAAGAGCACCAACAACAATGGAATTCCTGATTCCGTAGTATCCGAAAAAACCAATGTTCCTGATCCTGCTGGTAAAGTTCAGGTTCAATCTCTTTACCCCACTTTAGAAGTAGAATCCCTGTCCCGGGATCTGTTCTATCCTTCATTAGCTGAGGTTTCCAGTCCCGTACCAAAAATTCCAGTCCTTTCTCCAAAGCTTAGCACTCAATTTTCGCTTCCTCCTCCTCATCACCATCATCATCATCATCATCATCCTCCTCCTCCTCCTCCTCCATGTCAAGCATTCTCGCCATTTGTTCAACCTCCGAGGATGTACTGGCATCCATCAGAGTTCACAAATCATGGTTGCAATTTCTGTAGCAACCCTGCCGGTTTAGGACAAGTTGGGATGGTGGATTATAAGGGTCCATGGAGGACTAATTTTCCAGGGAATAGAAGTGATCTTCTTCAAGCTCCAGATCCGGAAACTACTACCAATGGCAGATGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

324

Amino Acids

36.73

Weight (kDa)

6.76

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 18 - 126 9.1e-07 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AatII GACGTC 1 cut(s) 129
Acc16I TGCGCA 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 349
Acc65I GGTACC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 361
AccI GTMKAC 1 cut(s) 204
AccIII TCCGGA 1 cut(s) 943
AclWI GGATC 5 cut(s) 87, 297, 539, 618, 935
AcsI RAATTY 3 cut(s) 30, 504, 657
AcuI CTGAAG 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 3 cut(s) 363, 651, 797
AfiI CCNNNNNNNGG 2 cut(s) 606, 607
AgsI TTSAA 7 cut(s) 56, 94, 370, 391, 569, 782, 932
AjnI CCWGG 1 cut(s) 907
AluBI AGCT 5 cut(s) 109, 249, 632, 679, 935
AluI AGCT 5 cut(s) 109, 249, 632, 679, 935
Alw21I GWGCWC 2 cut(s) 12, 491
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 5 cut(s) 87, 297, 539, 618, 935
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 943
ApeKI GCWGC 3 cut(s) 160, 181, 184
ApoI RAATTY 3 cut(s) 30, 504, 657
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
Asp718I GGTACC 1 cut(s) 361
AspLEI GCGC 2 cut(s) 189, 318
AspS9I GGNCC 1 cut(s) 887
AsuC2I CCSGG 3 cut(s) 227, 607, 608
AsuHPI GGTGA 2 cut(s) 443, 704
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 887
BanI GGYRCC 1 cut(s) 361
Bbv12I GWGCWC 2 cut(s) 12, 491
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 3 cut(s) 172, 193, 196
BccI CCATC 9 cut(s) 31, 140, 206, 212, 317, 428, 723, 814, 865
BciT130I CCWGG 1 cut(s) 909
BciVI GTATCC 1 cut(s) 532
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 3 cut(s) 227, 607, 608
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 2 cut(s) 179, 838
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 349
BfuI GTATCC 1 cut(s) 532
BisI GCNGC 3 cut(s) 161, 182, 185
BlpI GCTNAGC 1 cut(s) 680
BlsI GCNGC 3 cut(s) 162, 183, 186
Bme1390I CCNGG 4 cut(s) 227, 607, 608, 909
Bme18I GGWCC 1 cut(s) 887
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 3 cut(s) 224, 363, 888
BmrFI CCNGG 4 cut(s) 227, 607, 608, 909
BmsI GCATC 2 cut(s) 811, 956
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 680
Bpu14I TTCGAA 1 cut(s) 252
BpuMI CCSGG 3 cut(s) 227, 607, 608
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 5 cut(s) 346, 606, 788, 890, 908
BsaWI WCCGGW 1 cut(s) 943
Bsc4I CCNNNNNNNGG 2 cut(s) 606, 607
Bse118I RCCGGY 2 cut(s) 12, 851
Bse1I ACTGG 6 cut(s) 200, 299, 433, 642, 662, 803
Bse3DI GCAATG 1 cut(s) 325
BseAI TCCGGA 1 cut(s) 943
BseBI CCWGG 1 cut(s) 909
BseDI CCNNGG 5 cut(s) 346, 606, 788, 890, 908
BseGI GGATG 4 cut(s) 733, 798, 802, 876
BseLI CCNNNNNNNGG 2 cut(s) 606, 607
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 6 cut(s) 200, 299, 433, 642, 662, 803
BseRI GAGGAG 8 cut(s) 398, 693, 696, 729, 732, 735, 738, 741
BseXI GCAGC 3 cut(s) 172, 193, 196
BsgI GTGCAG 2 cut(s) 348, 377
BshNI GGYRCC 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 12, 491
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 5 cut(s) 13, 227, 607, 852, 944
BslFI GGGAC 2 cut(s) 589, 630
BslI CCNNNNNNNGG 2 cut(s) 606, 607
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 589, 630
BsmI GAATGC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 2 cut(s) 12, 491
Bsp13I TCCGGA 1 cut(s) 943
Bsp143I GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
Bsp1720I GCTNAGC 1 cut(s) 680
Bsp19I CCATGG 1 cut(s) 890
BspCNI CTCAG 1 cut(s) 625
BspEI TCCGGA 1 cut(s) 943
BspLI GGNNCC 3 cut(s) 224, 363, 888
BspMAI CTGCAG 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 5 cut(s) 87, 297, 539, 618, 935
BspQI GCTCTTC 2 cut(s) 335, 479
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 325
BsrFI RCCGGY 2 cut(s) 12, 851
BsrI ACTGG 6 cut(s) 200, 299, 433, 642, 662, 803
BssAI RCCGGY 2 cut(s) 12, 851
BssECI CCNNGG 5 cut(s) 346, 606, 788, 890, 908
BssMI GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 346, 890
Bst2UI CCWGG 1 cut(s) 909
Bst6I CTCTTC 2 cut(s) 335, 479
BstACI GRCGYC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 252
BstDEI CTNAG 3 cut(s) 454, 633, 680
BstDSI CCRYGG 1 cut(s) 890
BstF5I GGATG 4 cut(s) 733, 798, 802, 876
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 189, 318
BstKTI GATC 8 cut(s) 82, 283, 292, 381, 547, 613, 925, 943
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 909
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 4 cut(s) 225, 605, 606, 907
BstSFI CTRYAG 2 cut(s) 179, 838
BstV1I GCAGC 3 cut(s) 172, 193, 196
BstX2I RGATCY 3 cut(s) 79, 610, 940
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 3 cut(s) 79, 610, 940
BsuI GTATCC 1 cut(s) 532
BtgI CCRYGG 1 cut(s) 890
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 4 cut(s) 733, 798, 802, 876
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 52, 292
BveI ACCTGC 1 cut(s) 349
CfoI GCGC 2 cut(s) 189, 318
Cfr10I RCCGGY 2 cut(s) 12, 851
Cfr13I GGNCC 1 cut(s) 887
Cfr9I CCCGGG 1 cut(s) 606
Csp6I GTAC 3 cut(s) 362, 650, 796
CviAII CATG 6 cut(s) 265, 313, 470, 756, 824, 891
CviJI RGCY 9 cut(s) 16, 89, 109, 249, 263, 345, 632, 679, 935
CviKI_1 RGCY 9 cut(s) 16, 89, 109, 249, 263, 345, 632, 679, 935
CviQI GTAC 3 cut(s) 362, 650, 796
DdeI CTNAG 3 cut(s) 454, 633, 680
DpnI GATC 8 cut(s) 81, 282, 291, 380, 546, 612, 924, 942
DpnII GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
Eam1104I CTCTTC 2 cut(s) 335, 479
EarI CTCTTC 2 cut(s) 335, 479
Eco130I CCWWGG 2 cut(s) 346, 890
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 606
EcoRI GAATTC 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 907
EcoT14I CCWWGG 2 cut(s) 346, 890
ErhI CCWWGG 2 cut(s) 346, 890
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 6 cut(s) 268, 316, 473, 759, 827, 894
FaqI GGGAC 2 cut(s) 589, 630
FatI CATG 6 cut(s) 264, 312, 469, 755, 823, 890
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 161, 182, 185
FokI GGATG 4 cut(s) 720, 789, 805, 883
Fsp4HI GCNGC 3 cut(s) 161, 182, 185
FspBI CTAG 1 cut(s) 27
FspI TGCGCA 1 cut(s) 317
GlaI GCGC 2 cut(s) 188, 317
GluI GCNGC 3 cut(s) 161, 182, 185
GsuI CTGGAG 1 cut(s) 921
HaeII RGCGCY 1 cut(s) 190
HapII CCGG 5 cut(s) 13, 227, 607, 852, 944
HhaI GCGC 2 cut(s) 189, 318
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 6 cut(s) 268, 316, 473, 759, 827, 894
Hin6I GCGC 2 cut(s) 187, 316
HinP1I GCGC 2 cut(s) 187, 316
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 2 cut(s) 107, 677
HinfI GANTC 3 cut(s) 235, 512, 596
HpaII CCGG 5 cut(s) 13, 227, 607, 852, 944
HphI GGTGA 2 cut(s) 443, 704
Hpy166II GTNNAC 2 cut(s) 205, 816
Hpy188I TCNGA 3 cut(s) 526, 789, 811
Hpy188III TCNNGA 7 cut(s) 103, 151, 424, 509, 542, 938, 944
Hpy8I GTNNAC 2 cut(s) 205, 816
HpyAV CCTTC 3 cut(s) 55, 100, 633
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 6 cut(s) 45, 122, 181, 329, 358, 831
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 3 cut(s) 454, 633, 680
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 6 cut(s) 268, 316, 473, 759, 827, 894
HspAI GCGC 2 cut(s) 187, 316
Kpn2I TCCGGA 1 cut(s) 943
KpnI GGTACC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
LguI GCTCTTC 2 cut(s) 335, 479
LmnI GCTCC 2 cut(s) 395, 940
Lsp1109I GCAGC 3 cut(s) 172, 193, 196
LweI GCATC 2 cut(s) 811, 956
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 126
MalI GATC 8 cut(s) 81, 282, 291, 380, 546, 612, 924, 942
MboI GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
MboII GAAGA 6 cut(s) 91, 352, 493, 496, 917, 920
MflI RGATCY 3 cut(s) 79, 610, 940
MhlI GDGCHC 2 cut(s) 12, 491
MluCI AATT 8 cut(s) 30, 94, 474, 504, 657, 689, 832, 901
MroI TCCGGA 1 cut(s) 943
MspI CCGG 5 cut(s) 13, 227, 607, 852, 944
MspR9I CCNGG 4 cut(s) 227, 607, 608, 909
Mva1269I GAATGC 1 cut(s) 764
MvaI CCWGG 1 cut(s) 909
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 3 cut(s) 227, 607, 608
NcoI CCATGG 1 cut(s) 890
NdeII GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
NlaIII CATG 6 cut(s) 268, 316, 473, 759, 827, 894
NlaIV GGNNCC 3 cut(s) 224, 363, 888
NsbI TGCGCA 1 cut(s) 317
NspI RCATGY 1 cut(s) 316
NspV TTCGAA 1 cut(s) 252
PciSI GCTCTTC 2 cut(s) 335, 479
PctI GAATGC 1 cut(s) 764
PfeI GAWTC 3 cut(s) 235, 512, 596
PkrI GCNGC 3 cut(s) 162, 183, 186
PsiI TTATAA 1 cut(s) 882
Psp6I CCWGG 1 cut(s) 907
PspGI CCWGG 1 cut(s) 907
PspN4I GGNNCC 3 cut(s) 224, 363, 888
PspPI GGNCC 1 cut(s) 887
PsrI GAACNNNNNNTAC 2 cut(s) 290, 322
PstI CTGCAG 1 cut(s) 183
PsuI RGATCY 3 cut(s) 79, 610, 940
RsaI GTAC 3 cut(s) 363, 651, 797
RsaNI GTAC 3 cut(s) 362, 650, 796
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 2 cut(s) 335, 479
SatI GCNGC 3 cut(s) 161, 182, 185
Sau3AI GATC 8 cut(s) 79, 280, 289, 378, 544, 610, 922, 940
Sau96I GGNCC 1 cut(s) 887
ScrFI CCNGG 4 cut(s) 227, 607, 608, 909
SduI GDGCHC 2 cut(s) 12, 491
SfaNI GCATC 2 cut(s) 811, 956
SfcI CTRYAG 2 cut(s) 179, 838
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 1 cut(s) 887
SmaI CCCGGG 1 cut(s) 608
Sse9I AATT 8 cut(s) 30, 94, 474, 504, 657, 689, 832, 901
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 4 cut(s) 225, 605, 606, 907
StyI CCWWGG 2 cut(s) 346, 890
TaiI ACGT 1 cut(s) 129
TaqI TCGA 5 cut(s) 204, 252, 276, 309, 381
TasI AATT 8 cut(s) 30, 94, 474, 504, 657, 689, 832, 901
TatI WGTACW 1 cut(s) 795
TfiI GAWTC 3 cut(s) 235, 512, 596
TscAI CASTG 2 cut(s) 52, 299
TseI GCWGC 3 cut(s) 160, 181, 184
TspDTI ATGAA 2 cut(s) 159, 615
TspGWI ACGGA 1 cut(s) 505
TspMI CCCGGG 1 cut(s) 606
TspRI CASTG 2 cut(s) 52, 299
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 3 cut(s) 30, 504, 657
XceI RCATGY 1 cut(s) 316
XmaI CCCGGG 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 27
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.