RchiOBHm_Chr4g0428431

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004072
Physical Location & Seq
Reverse (-)
53496936 .. 53498254
1319 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39734

Sequence Viewer

Length: 942 bp
ATGGATTGCAGTGCTATTGAACTGAAGGTTATCTCCTGTAAGGATCTCAAAGCCTTCAATTTCTTCCAGAAGCTTTCAGTCTATGCAGACGTCTCCATCTCCAATGATGAAATCAAGAAAGAGCAGCAACAGCAAGAACATCTGCTGCAGCGCCAGAAAACACCAGTCGACAGAGATGGAGATGGGAACCCGGAGTGGAATCATACGATGAGCTTCGATACGAAAGCCATGTCATCACTCGATGATCAATGGGATCACTGGTATATCAAGTTCAACATGCGCAATGATGGTGCGGTTTTGGGGAAGAGCCTTGGCAAAGTGCAGGTACCTTTGAAAGAGTTGATCGAGGAGTTCAATGGAGCAGTCAGGTTTATGAGCTATCAAGTCAAGACTGGTGATGGGAAGTCTAATGGGGTCTTAGATTTTTCTTACCATGTAATTGGGAAGAAGAGCACCAACAACAATGGAATTCCTGATTCCGTAGTATCCGAAAAAACCAATGTTCCTGATCCTGCTGGTAAAGTTCAGGTTCAATCTCTTTACCCCACTTTAGAAGTAGAATCCCTGTCCCGGGATCTGTTCTATCCTTCATTAGCTGAGGTTTCCAGTCCCGTACCAAAAATTCCAGTCCCTTCTCCAAAGCTTAGCACTCAATTTTCGCTTCCTCCTCATCATCATCATCATCATCATCATCCTCCTCCTCCTCCTCCTCCTCCTCCTCCATGTCAAGCATTCTCGCCATTTGTTCAACCTCCGAGGATGTACTGGCATCCATCAGAGTTCACAAATCATGGTTGCAATTTCTGTAGCAACCCTGCTGGGTTAGGACAAGTTGGGATGGTGGATCATAAGGGTCCATGGAGGACTAATTTTCCAGGGAATAGAAGTGATCTTCTTCAAGCTCCAGATCCGGAAACTACTACCAATGGCAGATGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

313

Amino Acids

35.09

Weight (kDa)

6.42

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 115 3.1e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
Acc16I TGCGCA 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 313
Acc65I GGTACC 1 cut(s) 325
AccB1I GGYRCC 1 cut(s) 325
AccI GTMKAC 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 910
AciI CCGC 1 cut(s) 293
AclWI GGATC 6 cut(s) 51, 261, 503, 582, 852, 902
AcsI RAATTY 2 cut(s) 468, 621
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 327, 615, 764
AfiI CCNNNNNNNGG 2 cut(s) 570, 571
AgsI TTSAA 8 cut(s) 20, 58, 274, 334, 355, 533, 749, 899
AjnI CCWGG 1 cut(s) 874
AluBI AGCT 6 cut(s) 73, 213, 378, 596, 643, 902
AluI AGCT 6 cut(s) 73, 213, 378, 596, 643, 902
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 6 cut(s) 51, 261, 503, 582, 852, 902
Ama87I CYCGRG 1 cut(s) 570
Aor13HI TCCGGA 1 cut(s) 910
ApeKI GCWGC 3 cut(s) 124, 145, 148
ApoI RAATTY 2 cut(s) 468, 621
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
Asp718I GGTACC 1 cut(s) 325
AspLEI GCGC 2 cut(s) 153, 282
AspS9I GGNCC 1 cut(s) 854
AsuC2I CCSGG 3 cut(s) 191, 571, 572
AsuHPI GGTGA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 570
AvaII GGWCC 1 cut(s) 854
BanI GGYRCC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 455
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 132, 136, 160
BccI CCATC 7 cut(s) 104, 170, 176, 281, 392, 781, 832
BciT130I CCWGG 1 cut(s) 876
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 3 cut(s) 191, 571, 572
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 2 cut(s) 146, 805
BfoI RGCGCY 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 313
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 3 cut(s) 125, 146, 149
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 3 cut(s) 126, 147, 150
Bme1390I CCNGG 4 cut(s) 191, 571, 572, 876
Bme18I GGWCC 1 cut(s) 854
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 1 cut(s) 854
BmiI GGNNCC 3 cut(s) 188, 327, 855
BmrFI CCNGG 4 cut(s) 191, 571, 572, 876
BmsI GCATC 2 cut(s) 778, 923
BpmI CTGGAG 1 cut(s) 888
Bpu10I CCTNAGC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 644
BpuMI CCSGG 3 cut(s) 191, 571, 572
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 310, 570, 755, 857, 875
BsaWI WCCGGW 1 cut(s) 910
Bsc4I CCNNNNNNNGG 2 cut(s) 570, 571
Bse1I ACTGG 6 cut(s) 164, 263, 397, 606, 626, 770
Bse3DI GCAATG 1 cut(s) 289
BseAI TCCGGA 1 cut(s) 910
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 5 cut(s) 310, 570, 755, 857, 875
BseGI GGATG 4 cut(s) 691, 765, 769, 843
BseLI CCNNNNNNNGG 2 cut(s) 570, 571
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 6 cut(s) 164, 263, 397, 606, 626, 770
BseXI GCAGC 3 cut(s) 132, 136, 160
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 1 cut(s) 341
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 455
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 3 cut(s) 191, 571, 911
BslFI GGGAC 3 cut(s) 553, 594, 614
BslI CCNNNNNNNGG 2 cut(s) 570, 571
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 3 cut(s) 553, 594, 614
BsmI GAATGC 1 cut(s) 731
BsoBI CYCGRG 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 910
Bsp143I GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
Bsp1720I GCTNAGC 1 cut(s) 644
Bsp19I CCATGG 1 cut(s) 857
BspACI CCGC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 589
BspEI TCCGGA 1 cut(s) 910
BspLI GGNNCC 3 cut(s) 188, 327, 855
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 6 cut(s) 51, 261, 503, 582, 852, 902
BspQI GCTCTTC 2 cut(s) 299, 443
BspT107I GGYRCC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 289
BsrI ACTGG 6 cut(s) 164, 263, 397, 606, 626, 770
BssECI CCNNGG 5 cut(s) 310, 570, 755, 857, 875
BssMI GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 2 cut(s) 310, 857
Bst2UI CCWGG 1 cut(s) 876
Bst6I CTCTTC 2 cut(s) 299, 443
BstACI GRCGYC 1 cut(s) 90
BstDEI CTNAG 3 cut(s) 418, 597, 644
BstDSI CCRYGG 1 cut(s) 857
BstF5I GGATG 4 cut(s) 691, 765, 769, 843
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 2 cut(s) 153, 282
BstKTI GATC 9 cut(s) 46, 247, 256, 345, 511, 577, 847, 892, 910
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 4 cut(s) 189, 569, 570, 874
BstSFI CTRYAG 2 cut(s) 146, 805
BstV1I GCAGC 3 cut(s) 132, 136, 160
BstX2I RGATCY 3 cut(s) 43, 574, 907
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 3 cut(s) 43, 574, 907
BsuI GTATCC 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 857
BtsCI GGATG 4 cut(s) 691, 765, 769, 843
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 2 cut(s) 16, 256
BveI ACCTGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 153, 282
Cfr13I GGNCC 1 cut(s) 854
Cfr9I CCCGGG 1 cut(s) 570
Csp6I GTAC 3 cut(s) 326, 614, 763
CviAII CATG 6 cut(s) 229, 277, 434, 723, 791, 858
CviJI RGCY 9 cut(s) 53, 73, 213, 227, 309, 378, 596, 643, 902
CviKI_1 RGCY 9 cut(s) 53, 73, 213, 227, 309, 378, 596, 643, 902
CviQI GTAC 3 cut(s) 326, 614, 763
DdeI CTNAG 3 cut(s) 418, 597, 644
DpnI GATC 9 cut(s) 45, 246, 255, 344, 510, 576, 846, 891, 909
DpnII GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
Eam1104I CTCTTC 2 cut(s) 299, 443
EarI CTCTTC 2 cut(s) 299, 443
Eco130I CCWWGG 2 cut(s) 310, 857
Eco47I GGWCC 1 cut(s) 854
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 570
EcoRI GAATTC 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 874
EcoT14I CCWWGG 2 cut(s) 310, 857
ErhI CCWWGG 2 cut(s) 310, 857
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 6 cut(s) 232, 280, 437, 726, 794, 861
FaqI GGGAC 3 cut(s) 553, 594, 614
FatI CATG 6 cut(s) 228, 276, 433, 722, 790, 857
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 125, 146, 149
FokI GGATG 4 cut(s) 678, 756, 772, 850
Fsp4HI GCNGC 3 cut(s) 125, 146, 149
FspI TGCGCA 1 cut(s) 281
GlaI GCGC 2 cut(s) 152, 281
GluI GCNGC 3 cut(s) 125, 146, 149
GsaI CCCAGC 1 cut(s) 822
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 154
HapII CCGG 3 cut(s) 191, 571, 911
HhaI GCGC 2 cut(s) 153, 282
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 6 cut(s) 232, 280, 437, 726, 794, 861
Hin6I GCGC 2 cut(s) 151, 280
HinP1I GCGC 2 cut(s) 151, 280
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 71, 641
HinfI GANTC 3 cut(s) 199, 476, 560
HpaII CCGG 3 cut(s) 191, 571, 911
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 169, 783
Hpy188I TCNGA 3 cut(s) 490, 756, 778
Hpy188III TCNNGA 7 cut(s) 67, 115, 388, 473, 506, 905, 911
Hpy8I GTNNAC 2 cut(s) 169, 783
HpyAV CCTTC 4 cut(s) 19, 64, 597, 642
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 5 cut(s) 9, 86, 148, 322, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 3 cut(s) 418, 597, 644
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 6 cut(s) 232, 280, 437, 726, 794, 861
HspAI GCGC 2 cut(s) 151, 280
Kpn2I TCCGGA 1 cut(s) 910
KpnI GGTACC 1 cut(s) 329
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
LguI GCTCTTC 2 cut(s) 299, 443
LmnI GCTCC 2 cut(s) 359, 907
Lsp1109I GCAGC 3 cut(s) 132, 136, 160
LweI GCATC 2 cut(s) 778, 923
MaeII ACGT 1 cut(s) 90
MalI GATC 9 cut(s) 45, 246, 255, 344, 510, 576, 846, 891, 909
MboI GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
MboII GAAGA 6 cut(s) 55, 316, 457, 460, 884, 887
MflI RGATCY 3 cut(s) 43, 574, 907
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 7 cut(s) 58, 438, 468, 621, 653, 799, 868
MroI TCCGGA 1 cut(s) 910
MspI CCGG 3 cut(s) 191, 571, 911
MspR9I CCNGG 4 cut(s) 191, 571, 572, 876
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 1 cut(s) 876
MwoI GCNNNNNNNGC 1 cut(s) 130
NciI CCSGG 3 cut(s) 191, 571, 572
NcoI CCATGG 1 cut(s) 857
NdeII GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
NlaIII CATG 6 cut(s) 232, 280, 437, 726, 794, 861
NlaIV GGNNCC 3 cut(s) 188, 327, 855
NsbI TGCGCA 1 cut(s) 281
NspI RCATGY 1 cut(s) 280
PciSI GCTCTTC 2 cut(s) 299, 443
PctI GAATGC 1 cut(s) 731
PfeI GAWTC 3 cut(s) 199, 476, 560
PkrI GCNGC 3 cut(s) 126, 147, 150
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 3 cut(s) 188, 327, 855
PspPI GGNCC 1 cut(s) 854
PsrI GAACNNNNNNTAC 2 cut(s) 254, 286
PstI CTGCAG 1 cut(s) 150
PsuI RGATCY 3 cut(s) 43, 574, 907
RsaI GTAC 3 cut(s) 327, 615, 764
RsaNI GTAC 3 cut(s) 326, 614, 763
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 2 cut(s) 299, 443
SatI GCNGC 3 cut(s) 125, 146, 149
Sau3AI GATC 9 cut(s) 43, 244, 253, 342, 508, 574, 844, 889, 907
Sau96I GGNCC 1 cut(s) 854
ScrFI CCNGG 4 cut(s) 191, 571, 572, 876
SduI GDGCHC 1 cut(s) 455
SfaNI GCATC 2 cut(s) 778, 923
SfcI CTRYAG 2 cut(s) 146, 805
SinI GGWCC 1 cut(s) 854
SmaI CCCGGG 1 cut(s) 572
Sse9I AATT 7 cut(s) 58, 438, 468, 621, 653, 799, 868
SsiI CCGC 1 cut(s) 293
StyD4I CCNGG 4 cut(s) 189, 569, 570, 874
StyI CCWWGG 2 cut(s) 310, 857
TaiI ACGT 1 cut(s) 93
TaqI TCGA 4 cut(s) 168, 216, 240, 345
TasI AATT 7 cut(s) 58, 438, 468, 621, 653, 799, 868
TatI WGTACW 1 cut(s) 762
TfiI GAWTC 3 cut(s) 199, 476, 560
TscAI CASTG 2 cut(s) 16, 263
TseI GCWGC 3 cut(s) 124, 145, 148
TspDTI ATGAA 2 cut(s) 123, 579
TspGWI ACGGA 1 cut(s) 469
TspMI CCCGGG 1 cut(s) 570
TspRI CASTG 2 cut(s) 16, 263
VpaK11BI GGWCC 1 cut(s) 854
XapI RAATTY 2 cut(s) 468, 621
XceI RCATGY 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 570
XmiI GTMKAC 1 cut(s) 168
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.