MD16G1144400.v1.1

Emopamil binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004072
Physical Location & Seq
Forward (+)
11106719 .. 11108612
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1144400.v1.1.491

Sequence Viewer

Length: 495 bp
ATGCGCTGGTGGGCTTTCACCGGCCTTACAGACTTGATACTCGAGGGCTATTTTGCGATTGCGCCTGAGTTCTACAAGGATAAGACTGCTTGGTACCCGGCTGAAGTTTGGAAAGAGTATAGCAAAGGTGATTCAAGATATGCAGCTAGGGATGCAGGCGTCGTTGCTGTTGAAGGATTAACTGCAGTTATTGAAGGTCCGGCTAGCCTTCTTGCTGTGTATGCAATATCTAAAGGCAAGTCATATAGCTACATACTTCAGTTTGCCATTTCGTTGGGTCAGCTCTATGGAACTGCTGTGTACTTCATAACAGCCTACTTGGATGGTGACCAATTTGCTACAAGTTCGTTTTACTACTATGCATACTACATTGCTGCAAACGCCTCCTGGGTTGTAATACCGACGCTCATTTCCATCCGCTGTTGGAAGAAGATTTCTGCTGCAGTACAAGCTCAAGGCCAAGGCCAAGGTCAGAAAAAGAACAAAACTCGCTGA

Protein Analysis

165

Amino Acids

18.3

Weight (kDa)

9.01

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 34 - 141 8.5e-23 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 93
AciI CCGC 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 123, 242
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 3 cut(s) 95, 302, 447
AgsI TTSAA 3 cut(s) 135, 173, 194
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 4 cut(s) 146, 249, 283, 452
AluI AGCT 4 cut(s) 146, 249, 283, 452
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 3 cut(s) 22, 457, 463
ApeKI GCWGC 3 cut(s) 143, 374, 440
Asp718I GGTACC 1 cut(s) 93
AspLEI GCGC 2 cut(s) 6, 64
AspS9I GGNCC 1 cut(s) 197
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 3 cut(s) 10, 140, 338
AsuNHI GCTAGC 1 cut(s) 203
AvaI CYCGRG 1 cut(s) 41
AvaII GGWCC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 93
BbvI GCAGC 3 cut(s) 155, 361, 427
BccI CCATC 2 cut(s) 317, 422
BciT130I CCWGG 1 cut(s) 388
BcnI CCSGG 1 cut(s) 98
BfaI CTAG 2 cut(s) 147, 204
BfmI CTRYAG 2 cut(s) 183, 441
BisI GCNGC 3 cut(s) 144, 375, 441
BlsI GCNGC 3 cut(s) 145, 376, 442
Bme1390I CCNGG 2 cut(s) 98, 388
Bme18I GGWCC 1 cut(s) 197
BmeT110I CYCGRG 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 2 cut(s) 98, 388
BmsI GCATC 1 cut(s) 142
BmtI GCTAGC 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 387, 460, 466
Bse118I RCCGGY 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 387, 460, 466
BseGI GGATG 3 cut(s) 157, 328, 414
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 155, 361, 427
BshFI GGCC 3 cut(s) 24, 459, 465
BshNI GGYRCC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 3 cut(s) 21, 98, 200
BsnI GGCC 3 cut(s) 24, 459, 465
BsoBI CYCGRG 1 cut(s) 41
BspACI CCGC 1 cut(s) 418
BspANI GGCC 3 cut(s) 24, 459, 465
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 95
BspMAI CTGCAG 2 cut(s) 187, 445
BspOI GCTAGC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 3 cut(s) 387, 460, 466
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 2 cut(s) 460, 466
Bst2UI CCWGG 1 cut(s) 388
BstACI GRCGYC 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 157, 205
BstDEI CTNAG 1 cut(s) 66
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 3 cut(s) 157, 328, 414
BstHHI GCGC 2 cut(s) 6, 64
BstMWI GCNNNNNNNGC 4 cut(s) 152, 221, 380, 449
BstNI CCWGG 1 cut(s) 388
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 96, 386
BstSFI CTRYAG 2 cut(s) 183, 441
BstV1I GCAGC 3 cut(s) 155, 361, 427
BstXI CCANNNNNNTGG 1 cut(s) 274
BsuRI GGCC 3 cut(s) 24, 459, 465
BtsCI GGATG 3 cut(s) 157, 328, 414
Cac8I GCNNGC 2 cut(s) 157, 205
CfoI GCGC 2 cut(s) 6, 64
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 197
CseI GACGC 2 cut(s) 148, 412
Csp6I GTAC 3 cut(s) 94, 301, 446
CviQI GTAC 3 cut(s) 94, 301, 446
DdeI CTNAG 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 460, 466
Eco47I GGWCC 1 cut(s) 197
Eco57I CTGAAG 2 cut(s) 123, 242
Eco88I CYCGRG 1 cut(s) 41
Eco91I GGTNACC 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 2 cut(s) 460, 466
EcoT22I ATGCAT 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 460, 466
Fnu4HI GCNGC 3 cut(s) 144, 375, 441
FokI GGATG 3 cut(s) 164, 335, 401
Fsp4HI GCNGC 3 cut(s) 144, 375, 441
FspBI CTAG 2 cut(s) 147, 204
GlaI GCGC 2 cut(s) 5, 63
GluI GCNGC 3 cut(s) 144, 375, 441
HaeIII GGCC 3 cut(s) 24, 459, 465
HapII CCGG 3 cut(s) 21, 98, 200
HgaI GACGC 2 cut(s) 148, 412
HhaI GCGC 2 cut(s) 6, 64
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 2 cut(s) 4, 62
HinP1I GCGC 2 cut(s) 4, 62
HinfI GANTC 1 cut(s) 131
HpaII CCGG 3 cut(s) 21, 98, 200
HphI GGTGA 3 cut(s) 10, 140, 338
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 1 cut(s) 135
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 2 cut(s) 164, 406
HpyAV CCTTC 3 cut(s) 167, 188, 218
HpyCH4V TGCA 7 cut(s) 143, 155, 185, 224, 362, 377, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 221, 380, 449
HpyF3I CTNAG 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 2 cut(s) 4, 62
KpnI GGTACC 1 cut(s) 97
LpnPI CCDG 7 cut(s) 34, 78, 111, 141, 213, 373, 400
Lsp1109I GCAGC 3 cut(s) 155, 361, 427
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 147, 204
MaeIII GTNAC 1 cut(s) 326
MboII GAAGA 2 cut(s) 439, 442
MluCI AATT 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 2 cut(s) 37, 394
Mph1103I ATGCAT 1 cut(s) 364
MseI TTAA 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 3 cut(s) 21, 98, 200
MspR9I CCNGG 2 cut(s) 98, 388
MvaI CCWGG 1 cut(s) 388
MwoI GCNNNNNNNGC 4 cut(s) 152, 221, 380, 449
NciI CCSGG 1 cut(s) 98
NheI GCTAGC 1 cut(s) 203
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 364
PaeR7I CTCGAG 1 cut(s) 41
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 3 cut(s) 145, 376, 442
Psp6I CCWGG 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 326
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 197
PspXI VCTCGAGB 1 cut(s) 41
PstI CTGCAG 2 cut(s) 187, 445
RsaI GTAC 3 cut(s) 95, 302, 447
RsaNI GTAC 3 cut(s) 94, 301, 446
SaqAI TTAA 1 cut(s) 179
SatI GCNGC 3 cut(s) 144, 375, 441
Sau96I GGNCC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 98, 388
SetI ASST 7 cut(s) 130, 148, 199, 251, 285, 454, 472
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 2 cut(s) 183, 441
Sfr274I CTCGAG 1 cut(s) 41
SinI GGWCC 1 cut(s) 197
SlaI CTCGAG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 41, 453
SmoI CTYRAG 2 cut(s) 41, 453
Sse9I AATT 1 cut(s) 332
SsiI CCGC 1 cut(s) 418
SspMI CTAG 2 cut(s) 147, 204
StyD4I CCNGG 2 cut(s) 96, 386
StyI CCWWGG 2 cut(s) 460, 466
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 332
TatI WGTACW 2 cut(s) 300, 445
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 3 cut(s) 143, 374, 440
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 41
XspI CTAG 2 cut(s) 147, 204
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.