Rw4G024540

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004072
Physical Location & Seq
Reverse (-)
50381848 .. 50383079
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024540.1

Sequence Viewer

Length: 909 bp
ATGTTTGAGCACCGGCTGAAACAAACTAGAATTTTGATGGATTGCAGTGCTATTGAACTGAAGGTTATCTCCTGTAAGGATCTCAAAGCCTTCAATTTCTTCCAGAAGCTTTCAGTCTATGCAGACGTCTCCATCTCCAATGATGAAATCAAGAAAGAGCAGCAACAGCAAGAACATCTGCAGCAGCGCCAGAAAACACCAGTCGACAGAGATGGAGATGGGAACCCGGAGTGGAATCATACGATGAGCTTCGAACGAAAGCCATTTTTGGGGAAGAGCCTTGGCAAAGTGCAGGTACCTTTGAAAGAGTTGATCGAGGAGTTCAATGGAGCAGTCAGGTTTATGAGGTATCAAGTCAAGACTGGTGATGGGAAGTCTAATGGGGTCTTAGATTTTTCTTACCATGTAATTGGGAAGAAGAGCACCAACAACAATGGAATTCCTGATTCCGTAGTATCCGAAAAAACCAATGTTCCTGATCCTGCTGGTAAAGTTCAGGTTCAATCTCTTTACCCCACTTTAGAAGTAGAATCCCTGTCCCGGGATCTGTTCTATCCTTCATTAGCTGAGGTTTCCAGTCCCGTACCAAAAATTCCAGTCCTTTCTCCAAAGCTTAGCACTCAATTTTCGCTTCCTCCTCCTCATCACCATCATCATCATCATCATCATCCTCCTCCTCCTCCTCCTCCATGTCAAGCATTCTCGCCATTTGTTCAACCTCCGAGGATGTACTGGCATCCATCAGAGTTCACAAATCATGGTTGCAATTTCTGTAGCAACCCTGCCGGTTTAGGACAAGTTGGGATGGTGGATTATAAGGGTCCATGGAGGACTAATTTTCCAGGGAATAGAAGTGATCTTCTTCAAGCTCCAGATCCGGAAACTACTACCAATGGCAGATGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

302

Amino Acids

34.14

Weight (kDa)

8.16

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 18 - 88 5.7e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
AatII GACGTC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 283
Acc65I GGTACC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 204
AccIII TCCGGA 1 cut(s) 877
AclWI GGATC 4 cut(s) 87, 473, 552, 869
AcsI RAATTY 3 cut(s) 30, 438, 591
AcuI CTGAAG 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 3 cut(s) 297, 585, 731
AfiI CCNNNNNNNGG 3 cut(s) 269, 540, 541
AgsI TTSAA 7 cut(s) 56, 94, 304, 325, 503, 716, 866
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 5 cut(s) 109, 249, 566, 613, 869
AluI AGCT 5 cut(s) 109, 249, 566, 613, 869
Alw21I GWGCWC 2 cut(s) 12, 425
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 4 cut(s) 87, 473, 552, 869
Ama87I CYCGRG 1 cut(s) 540
Aor13HI TCCGGA 1 cut(s) 877
ApeKI GCWGC 3 cut(s) 160, 181, 184
ApoI RAATTY 3 cut(s) 30, 438, 591
Asp718I GGTACC 1 cut(s) 295
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 821
AsuC2I CCSGG 3 cut(s) 227, 541, 542
AsuHPI GGTGA 2 cut(s) 377, 638
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 1 cut(s) 821
BanI GGYRCC 1 cut(s) 295
Bbv12I GWGCWC 2 cut(s) 12, 425
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 3 cut(s) 172, 193, 196
BccI CCATC 8 cut(s) 31, 140, 206, 212, 362, 657, 748, 799
BciT130I CCWGG 1 cut(s) 843
BciVI GTATCC 1 cut(s) 466
BcnI CCSGG 3 cut(s) 227, 541, 542
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 2 cut(s) 179, 772
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 3 cut(s) 161, 182, 185
BlpI GCTNAGC 1 cut(s) 614
BlsI GCNGC 3 cut(s) 162, 183, 186
Bme1390I CCNGG 4 cut(s) 227, 541, 542, 843
Bme18I GGWCC 1 cut(s) 821
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 3 cut(s) 224, 297, 822
BmrFI CCNGG 4 cut(s) 227, 541, 542, 843
BmsI GCATC 2 cut(s) 745, 890
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 614
Bpu14I TTCGAA 1 cut(s) 252
BpuMI CCSGG 3 cut(s) 227, 541, 542
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 5 cut(s) 280, 540, 722, 824, 842
BsaWI WCCGGW 1 cut(s) 877
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 540, 541
Bse118I RCCGGY 2 cut(s) 12, 785
Bse1I ACTGG 5 cut(s) 200, 367, 576, 596, 737
BseAI TCCGGA 1 cut(s) 877
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 5 cut(s) 280, 540, 722, 824, 842
BseGI GGATG 4 cut(s) 667, 732, 736, 810
BseLI CCNNNNNNNGG 3 cut(s) 269, 540, 541
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 5 cut(s) 200, 367, 576, 596, 737
BseRI GAGGAG 8 cut(s) 332, 627, 630, 663, 666, 669, 672, 675
BseXI GCAGC 3 cut(s) 172, 193, 196
BsgI GTGCAG 1 cut(s) 311
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 2 cut(s) 12, 425
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 5 cut(s) 13, 227, 541, 786, 878
BslFI GGGAC 2 cut(s) 523, 564
BslI CCNNNNNNNGG 3 cut(s) 269, 540, 541
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 523, 564
BsmI GAATGC 1 cut(s) 698
BsoBI CYCGRG 1 cut(s) 540
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 2 cut(s) 12, 425
Bsp13I TCCGGA 1 cut(s) 877
Bsp143I GATC 6 cut(s) 79, 312, 478, 544, 856, 874
Bsp1720I GCTNAGC 1 cut(s) 614
Bsp19I CCATGG 1 cut(s) 824
BspCNI CTCAG 1 cut(s) 559
BspEI TCCGGA 1 cut(s) 877
BspLI GGNNCC 3 cut(s) 224, 297, 822
BspMAI CTGCAG 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 4 cut(s) 87, 473, 552, 869
BspQI GCTCTTC 2 cut(s) 269, 413
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 295
BsrFI RCCGGY 2 cut(s) 12, 785
BsrI ACTGG 5 cut(s) 200, 367, 576, 596, 737
BssAI RCCGGY 2 cut(s) 12, 785
BssECI CCNNGG 5 cut(s) 280, 540, 722, 824, 842
BssMI GATC 6 cut(s) 79, 312, 478, 544, 856, 874
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 280, 824
Bst2UI CCWGG 1 cut(s) 843
Bst6I CTCTTC 2 cut(s) 269, 413
BstACI GRCGYC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 252
BstDEI CTNAG 3 cut(s) 388, 567, 614
BstDSI CCRYGG 1 cut(s) 824
BstF5I GGATG 4 cut(s) 667, 732, 736, 810
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 6 cut(s) 82, 315, 481, 547, 859, 877
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 6 cut(s) 79, 312, 478, 544, 856, 874
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 843
BstSCI CCNGG 4 cut(s) 225, 539, 540, 841
BstSFI CTRYAG 2 cut(s) 179, 772
BstV1I GCAGC 3 cut(s) 172, 193, 196
BstX2I RGATCY 3 cut(s) 79, 544, 874
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 3 cut(s) 79, 544, 874
BsuI GTATCC 1 cut(s) 466
BtgI CCRYGG 1 cut(s) 824
BtsCI GGATG 4 cut(s) 667, 732, 736, 810
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
BveI ACCTGC 1 cut(s) 283
CfoI GCGC 1 cut(s) 189
Cfr10I RCCGGY 2 cut(s) 12, 785
Cfr13I GGNCC 1 cut(s) 821
Cfr9I CCCGGG 1 cut(s) 540
Csp6I GTAC 3 cut(s) 296, 584, 730
CviAII CATG 4 cut(s) 404, 690, 758, 825
CviJI RGCY 9 cut(s) 16, 89, 109, 249, 262, 279, 566, 613, 869
CviKI_1 RGCY 9 cut(s) 16, 89, 109, 249, 262, 279, 566, 613, 869
CviQI GTAC 3 cut(s) 296, 584, 730
DdeI CTNAG 3 cut(s) 388, 567, 614
DpnI GATC 6 cut(s) 81, 314, 480, 546, 858, 876
DpnII GATC 6 cut(s) 79, 312, 478, 544, 856, 874
Eam1104I CTCTTC 2 cut(s) 269, 413
EarI CTCTTC 2 cut(s) 269, 413
Eco130I CCWWGG 2 cut(s) 280, 824
Eco47I GGWCC 1 cut(s) 821
Eco57I CTGAAG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 540
EcoRI GAATTC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 2 cut(s) 280, 824
ErhI CCWWGG 2 cut(s) 280, 824
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 4 cut(s) 407, 693, 761, 828
FaiI YATR 8 cut(s) 120, 240, 344, 405, 691, 759, 816, 826
FaqI GGGAC 2 cut(s) 523, 564
FatI CATG 4 cut(s) 403, 689, 757, 824
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 161, 182, 185
FokI GGATG 4 cut(s) 654, 723, 739, 817
Fsp4HI GCNGC 3 cut(s) 161, 182, 185
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 188
GluI GCNGC 3 cut(s) 161, 182, 185
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 190
HapII CCGG 5 cut(s) 13, 227, 541, 786, 878
HhaI GCGC 1 cut(s) 189
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 407, 693, 761, 828
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 2 cut(s) 107, 611
HinfI GANTC 3 cut(s) 235, 446, 530
HpaII CCGG 5 cut(s) 13, 227, 541, 786, 878
HphI GGTGA 2 cut(s) 377, 638
Hpy166II GTNNAC 2 cut(s) 205, 750
Hpy188I TCNGA 3 cut(s) 460, 723, 745
Hpy188III TCNNGA 7 cut(s) 103, 151, 358, 443, 476, 872, 878
Hpy8I GTNNAC 2 cut(s) 205, 750
HpyAV CCTTC 3 cut(s) 55, 100, 567
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 5 cut(s) 45, 122, 181, 292, 765
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 3 cut(s) 388, 567, 614
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 407, 693, 761, 828
HspAI GCGC 1 cut(s) 187
Kpn2I TCCGGA 1 cut(s) 877
KpnI GGTACC 1 cut(s) 299
Kzo9I GATC 6 cut(s) 79, 312, 478, 544, 856, 874
LguI GCTCTTC 2 cut(s) 269, 413
LmnI GCTCC 2 cut(s) 329, 874
Lsp1109I GCAGC 3 cut(s) 172, 193, 196
LweI GCATC 2 cut(s) 745, 890
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 126
MalI GATC 6 cut(s) 81, 314, 480, 546, 858, 876
MboI GATC 6 cut(s) 79, 312, 478, 544, 856, 874
MboII GAAGA 6 cut(s) 91, 286, 427, 430, 851, 854
MflI RGATCY 3 cut(s) 79, 544, 874
MhlI GDGCHC 2 cut(s) 12, 425
MluCI AATT 8 cut(s) 30, 94, 408, 438, 591, 623, 766, 835
MroI TCCGGA 1 cut(s) 877
MspI CCGG 5 cut(s) 13, 227, 541, 786, 878
MspR9I CCNGG 4 cut(s) 227, 541, 542, 843
Mva1269I GAATGC 1 cut(s) 698
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 3 cut(s) 227, 541, 542
NcoI CCATGG 1 cut(s) 824
NdeII GATC 6 cut(s) 79, 312, 478, 544, 856, 874
NlaIII CATG 4 cut(s) 407, 693, 761, 828
NlaIV GGNNCC 3 cut(s) 224, 297, 822
NspV TTCGAA 1 cut(s) 252
PciSI GCTCTTC 2 cut(s) 269, 413
PctI GAATGC 1 cut(s) 698
PfeI GAWTC 3 cut(s) 235, 446, 530
PkrI GCNGC 3 cut(s) 162, 183, 186
PsiI TTATAA 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 3 cut(s) 224, 297, 822
PspPI GGNCC 1 cut(s) 821
PstI CTGCAG 1 cut(s) 183
PsuI RGATCY 3 cut(s) 79, 544, 874
RsaI GTAC 3 cut(s) 297, 585, 731
RsaNI GTAC 3 cut(s) 296, 584, 730
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 2 cut(s) 269, 413
SatI GCNGC 3 cut(s) 161, 182, 185
Sau3AI GATC 6 cut(s) 79, 312, 478, 544, 856, 874
Sau96I GGNCC 1 cut(s) 821
ScrFI CCNGG 4 cut(s) 227, 541, 542, 843
SduI GDGCHC 2 cut(s) 12, 425
SfaNI GCATC 2 cut(s) 745, 890
SfcI CTRYAG 2 cut(s) 179, 772
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 1 cut(s) 821
SmaI CCCGGG 1 cut(s) 542
Sse9I AATT 8 cut(s) 30, 94, 408, 438, 591, 623, 766, 835
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 4 cut(s) 225, 539, 540, 841
StyI CCWWGG 2 cut(s) 280, 824
TaiI ACGT 1 cut(s) 129
TaqI TCGA 3 cut(s) 204, 252, 315
TasI AATT 8 cut(s) 30, 94, 408, 438, 591, 623, 766, 835
TatI WGTACW 1 cut(s) 729
TfiI GAWTC 3 cut(s) 235, 446, 530
TscAI CASTG 1 cut(s) 52
TseI GCWGC 3 cut(s) 160, 181, 184
TspDTI ATGAA 2 cut(s) 159, 549
TspGWI ACGGA 1 cut(s) 439
TspMI CCCGGG 1 cut(s) 540
TspRI CASTG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 821
XapI RAATTY 3 cut(s) 30, 438, 591
XmaI CCCGGG 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 27
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.