pycom13g11660

Emopamil binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004072
Physical Location & Seq
Forward (+)
8042074 .. 8044594
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g11660.1

Sequence Viewer

Length: 780 bp
ATGAAGGTATACAATTCCGGCATTCAGGCACTTCTGCCTCGACATCTTCGAGTCCTCACGGTACAGTGTACTAGGCAGCGAGTGAAAATGGAGGGATCAGACTCTGGGATTTATGGGAATCATCCGTACGCCCCGAGGGATCTAAAGCTACCCGGATTCGTACCTGGATTTCTCTCACAGTCCACCATTCTTGGCATCTACGGCGTCTCCTCCCTACTCGTCGTTTCCCTCACCTGGCTCTTCTCCGGGAGATCACCGAGGAAATCGAAACTTGATAGATGGCTAATGTGTTGGTGGGCTTTCACCGGCCTTACGCACTTGATACTCGAAGGCTATTTCGCATTTTCGCCTGAGTTTTACAAGAATAAGACTGCTTGTTACCTGGCTGAAGTTTGGAAAGAGTATAGCAAAGGTGATTCAAGATATGCAGCTAGGGATGCAGGGGTCGTTTCTGTCGAAGGATTAACTGCAGTTATAGAAGGTCCCGCTAGCCTTCTTGCAGTATATGCAATATCTAAAGGCAAGCCATATAGCTACATACTTCAGTTTGCCATTTCATTGGGTCAGCTCTATGGAACTGCTGTATATTTCATAACAGCCTACTTGGAAGGTGACAAATTTGCTACAAATTCGTTTTACTACTACGCATACTACATTGCTGCAAACGCCTCCTGGGTTGTAATACCGACGCTCATTTCCATCCGCTGTTGGAAGAAGATTTCTGCAGCAGTACAAGTTCAAGCCCAAGGCCAAGACCAGAAAAAGAACAAAACTCGCTGA

Protein Analysis

260

Amino Acids

29.07

Weight (kDa)

9.56

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 128 - 236 2.7e-23 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 2 cut(s) 486, 703
AclWI GGATC 2 cut(s) 103, 147
AcsI RAATTY 2 cut(s) 617, 628
AcuI CTGAAG 2 cut(s) 408, 527
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 5 cut(s) 63, 70, 128, 162, 732
AfiI CCNNNNNNNGG 1 cut(s) 234
AgsI TTSAA 2 cut(s) 420, 740
AjnI CCWGG 4 cut(s) 163, 233, 381, 671
AluBI AGCT 4 cut(s) 148, 431, 534, 568
AluI AGCT 4 cut(s) 148, 431, 534, 568
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 2 cut(s) 103, 147
AlwNI CAGNNNCTG 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 2 cut(s) 307, 748
ApeKI GCWGC 4 cut(s) 76, 428, 659, 725
ApoI RAATTY 2 cut(s) 617, 628
AspS9I GGNCC 1 cut(s) 482
AsuC2I CCSGG 2 cut(s) 153, 247
AsuHPI GGTGA 5 cut(s) 223, 246, 295, 425, 623
AsuNHI GCTAGC 1 cut(s) 488
AvaI CYCGRG 1 cut(s) 133
AvaII GGWCC 1 cut(s) 482
BbvI GCAGC 4 cut(s) 88, 440, 646, 737
BccI CCATC 2 cut(s) 273, 707
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 4 cut(s) 165, 235, 383, 673
BcnI CCSGG 2 cut(s) 153, 247
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 3 cut(s) 72, 432, 489
BfmI CTRYAG 2 cut(s) 468, 723
BisI GCNGC 4 cut(s) 77, 429, 660, 726
BlsI GCNGC 4 cut(s) 78, 430, 661, 727
Bme1390I CCNGG 6 cut(s) 153, 165, 235, 247, 383, 673
Bme18I GGWCC 1 cut(s) 482
BmeT110I CYCGRG 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 6 cut(s) 153, 165, 235, 247, 383, 673
BmsI GCATC 2 cut(s) 204, 427
BmtI GCTAGC 1 cut(s) 492
BpuMI CCSGG 2 cut(s) 153, 247
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 4 cut(s) 134, 257, 672, 745
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse118I RCCGGY 1 cut(s) 305
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 4 cut(s) 165, 235, 383, 673
BseDI CCNNGG 4 cut(s) 134, 257, 672, 745
BseGI GGATG 3 cut(s) 121, 442, 699
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 1 cut(s) 342
BseRI GAGGAG 1 cut(s) 199
BseXI GCAGC 4 cut(s) 88, 440, 646, 737
BshFI GGCC 2 cut(s) 309, 750
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 4 cut(s) 18, 153, 246, 306
BsiWI CGTACG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 1 cut(s) 211
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 468
BsmI GAATGC 1 cut(s) 21
BsnI GGCC 2 cut(s) 309, 750
BsoBI CYCGRG 1 cut(s) 133
Bsp143I GATC 3 cut(s) 95, 139, 251
BspACI CCGC 2 cut(s) 486, 703
BspANI GGCC 2 cut(s) 309, 750
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 484
BspMAI CTGCAG 2 cut(s) 472, 727
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 2 cut(s) 103, 147
BspQI GCTCTTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 4 cut(s) 134, 257, 672, 745
BssMI GATC 3 cut(s) 95, 139, 251
BssNAI GTATAC 1 cut(s) 10
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 745
Bst1107I GTATAC 1 cut(s) 10
Bst2UI CCWGG 4 cut(s) 165, 235, 383, 673
Bst4CI ACNGT 3 cut(s) 61, 66, 180
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 490, 524
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 3 cut(s) 121, 442, 699
BstKTI GATC 3 cut(s) 98, 142, 254
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 3 cut(s) 95, 139, 251
BstMWI GCNNNNNNNGC 4 cut(s) 201, 437, 506, 665
BstNI CCWGG 4 cut(s) 165, 235, 383, 673
BstSCI CCNGG 6 cut(s) 151, 163, 233, 245, 381, 671
BstSFI CTRYAG 2 cut(s) 468, 723
BstV1I GCAGC 4 cut(s) 88, 440, 646, 737
BstX2I RGATCY 1 cut(s) 139
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 1 cut(s) 139
BstZ17I GTATAC 1 cut(s) 10
BsuRI GGCC 2 cut(s) 309, 750
BtsCI GGATG 3 cut(s) 121, 442, 699
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 2 cut(s) 490, 524
CaiI CAGNNNCTG 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 482
CseI GACGC 2 cut(s) 193, 697
Csp6I GTAC 5 cut(s) 62, 69, 127, 161, 731
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviQI GTAC 5 cut(s) 62, 69, 127, 161, 731
DdeI CTNAG 1 cut(s) 351
DpnI GATC 3 cut(s) 97, 141, 253
DpnII GATC 3 cut(s) 95, 139, 251
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 482
Eco57I CTGAAG 2 cut(s) 408, 527
Eco88I CYCGRG 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 482
EcoRII CCWGG 4 cut(s) 163, 233, 381, 671
EcoT14I CCWWGG 1 cut(s) 745
ErhI CCWWGG 1 cut(s) 745
Esp3I CGTCTC 1 cut(s) 211
FaqI GGGAC 1 cut(s) 468
FauI CCCGC 1 cut(s) 493
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 4 cut(s) 77, 429, 660, 726
FokI GGATG 3 cut(s) 108, 449, 686
Fsp4HI GCNGC 4 cut(s) 77, 429, 660, 726
FspBI CTAG 3 cut(s) 72, 432, 489
GluI GCNGC 4 cut(s) 77, 429, 660, 726
HaeIII GGCC 2 cut(s) 309, 750
HapII CCGG 4 cut(s) 18, 153, 246, 306
HgaI GACGC 2 cut(s) 193, 697
Hin1I GRCGYC 1 cut(s) 204
HinfI GANTC 5 cut(s) 51, 101, 118, 156, 416
HpaII CCGG 4 cut(s) 18, 153, 246, 306
HphI GGTGA 5 cut(s) 223, 246, 295, 425, 623
Hpy166II GTNNAC 3 cut(s) 10, 69, 183
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 1 cut(s) 420
Hpy8I GTNNAC 3 cut(s) 10, 69, 183
Hpy99I CGWCG 2 cut(s) 224, 691
HpyAV CCTTC 5 cut(s) 323, 452, 473, 503, 602
HpyCH4III ACNGT 3 cut(s) 61, 66, 180
HpyCH4V TGCA 7 cut(s) 428, 440, 470, 500, 509, 662, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 201, 437, 506, 665
HpyF3I CTNAG 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 204
Kzo9I GATC 3 cut(s) 95, 139, 251
LguI GCTCTTC 1 cut(s) 245
Lsp1109I GCAGC 4 cut(s) 88, 440, 646, 737
LweI GCATC 2 cut(s) 204, 427
MaeI CTAG 3 cut(s) 72, 432, 489
MaeIII GTNAC 2 cut(s) 377, 611
MalI GATC 3 cut(s) 97, 141, 253
MboI GATC 3 cut(s) 95, 139, 251
MboII GAAGA 4 cut(s) 38, 232, 724, 727
MflI RGATCY 1 cut(s) 139
MluCI AATT 3 cut(s) 13, 617, 628
MlyI GAGTC 2 cut(s) 60, 95
MmeI TCCRAC 1 cut(s) 689
MnlI CCTC 8 cut(s) 48, 65, 85, 129, 220, 239, 252, 679
MseI TTAA 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 705
MspI CCGG 4 cut(s) 18, 153, 246, 306
MspR9I CCNGG 6 cut(s) 153, 165, 235, 247, 383, 673
Mva1269I GAATGC 1 cut(s) 21
MvaI CCWGG 4 cut(s) 165, 235, 383, 673
MwoI GCNNNNNNNGC 4 cut(s) 201, 437, 506, 665
NciI CCSGG 2 cut(s) 153, 247
NdeII GATC 3 cut(s) 95, 139, 251
NheI GCTAGC 1 cut(s) 488
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 611
PciSI GCTCTTC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 2 cut(s) 46, 453
PctI GAATGC 1 cut(s) 21
PfeI GAWTC 3 cut(s) 118, 156, 416
Pfl23II CGTACG 1 cut(s) 126
PfoI TCCNGGA 1 cut(s) 245
PkrI GCNGC 4 cut(s) 78, 430, 661, 727
PleI GAGTC 2 cut(s) 59, 95
PpsI GAGTC 2 cut(s) 59, 95
PpuMI RGGWCCY 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 482
Psp6I CCWGG 4 cut(s) 163, 233, 381, 671
PspGI CCWGG 4 cut(s) 163, 233, 381, 671
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 1 cut(s) 482
PspPPI RGGWCCY 1 cut(s) 482
PstI CTGCAG 2 cut(s) 472, 727
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 5 cut(s) 63, 70, 128, 162, 732
RsaNI GTAC 5 cut(s) 62, 69, 127, 161, 731
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 464
SatI GCNGC 4 cut(s) 77, 429, 660, 726
Sau3AI GATC 3 cut(s) 95, 139, 251
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 2 cut(s) 60, 95
ScrFI CCNGG 6 cut(s) 153, 165, 235, 247, 383, 673
SfaNI GCATC 2 cut(s) 204, 427
SfcI CTRYAG 2 cut(s) 468, 723
SinI GGWCC 1 cut(s) 482
Sse9I AATT 3 cut(s) 13, 617, 628
SsiI CCGC 2 cut(s) 486, 703
SspMI CTAG 3 cut(s) 72, 432, 489
StyD4I CCNGG 6 cut(s) 151, 163, 233, 245, 381, 671
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 3 cut(s) 61, 66, 180
TaqI TCGA 5 cut(s) 40, 49, 266, 327, 456
TasI AATT 3 cut(s) 13, 617, 628
TatI WGTACW 2 cut(s) 68, 730
TfiI GAWTC 3 cut(s) 118, 156, 416
Tru1I TTAA 1 cut(s) 464
Tru9I TTAA 1 cut(s) 464
TscAI CASTG 1 cut(s) 71
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 4 cut(s) 76, 428, 659, 725
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 3 cut(s) 17, 546, 580
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 617, 628
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 3 cut(s) 72, 432, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.