MD13G1133800.v1.1

Emopamil binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004072
Physical Location & Seq
Forward (+)
10242813 .. 10244732
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1133800.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGAGGGATCAGACTCTGGGATTTATGGGAATCATCCGTACGCCCCGAGGGATCTAAAGCTACCCGGATTCGTACCCGGATTTCTCTCACAGTCCACCATTCTTGGCGTCTACGGCGTCTCCTCCCTACTCGTCGTTTCCCTCACCTGGCTCTTCTCCGGGAGGTCACCGAGGAAATCAAAACTTGAAAGATGGCTAATGTGTTGGTGGGCTTTCACCGGCCTTACGCACTTGATACTCGAAGGCTATTTTGCATTTTCGCCCGAGTTTTACAAGGATAAGACTGCTTGTTACCTGGCTGAAGTTTGGAAAGAGTATAGCAAAGGTGATTCAAGATATGCAGCGAGGGATGCAGGGGTCGTTTCTGTCGAAGGATTAACCGCAGTTATAGAAGGTCCCGCTAGCCTTCTTGCAGTATATGCAATATCTAAAGGCAAGCCATATAGCTACATACTTCAGTTTGCCATTTCGTTGGGTCAGCTCTATGGAACTGCTGTATATTTCATAACAGCCTACTTGGAAGGTGACAAATTTGCTACAAATTCGTTTTACTACTACGCATACTACATTGCTGCAAACGCCTCCTGGGTTGTAATACCGACGCTCATTTCCATCCGCTGTTGGAAGAAGATTTCTGCAGCAGTACAAGTTCAAGCCCAAGGCCAAGACCAGAAAAAGAACAAAACTCGCTGA

Protein Analysis

231

Amino Acids

25.68

Weight (kDa)

9.15

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 99 - 207 2e-23 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AciI CCGC 3 cut(s) 381, 399, 616
AclWI GGATC 2 cut(s) 16, 60
AcsI RAATTY 2 cut(s) 530, 541
AcuI CTGAAG 2 cut(s) 321, 440
AcyI GRCGYC 2 cut(s) 108, 117
AfaI GTAC 3 cut(s) 41, 75, 645
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 3 cut(s) 188, 333, 653
AjnI CCWGG 3 cut(s) 146, 294, 584
AluBI AGCT 3 cut(s) 61, 447, 481
AluI AGCT 3 cut(s) 61, 447, 481
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 2 cut(s) 16, 60
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 2 cut(s) 46, 263
AoxI GGCC 2 cut(s) 220, 661
ApeKI GCWGC 3 cut(s) 341, 572, 638
ApoI RAATTY 2 cut(s) 530, 541
AspS9I GGNCC 1 cut(s) 395
AsuC2I CCSGG 3 cut(s) 66, 78, 160
AsuHPI GGTGA 5 cut(s) 136, 159, 208, 338, 536
AsuNHI GCTAGC 1 cut(s) 401
AvaI CYCGRG 2 cut(s) 46, 263
AvaII GGWCC 1 cut(s) 395
BbvI GCAGC 3 cut(s) 353, 559, 650
BccI CCATC 2 cut(s) 186, 620
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 3 cut(s) 148, 296, 586
BcnI CCSGG 3 cut(s) 66, 78, 160
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 1 cut(s) 636
BisI GCNGC 3 cut(s) 342, 573, 639
BlsI GCNGC 3 cut(s) 343, 574, 640
Bme1390I CCNGG 6 cut(s) 66, 78, 148, 160, 296, 586
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 2 cut(s) 46, 263
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 6 cut(s) 66, 78, 148, 160, 296, 586
BmsI GCATC 1 cut(s) 340
BmtI GCTAGC 1 cut(s) 405
BpuMI CCSGG 3 cut(s) 66, 78, 160
BsaHI GRCGYC 2 cut(s) 108, 117
BsaJI CCNNGG 4 cut(s) 47, 170, 585, 658
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse118I RCCGGY 1 cut(s) 218
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 3 cut(s) 148, 296, 586
BseDI CCNNGG 4 cut(s) 47, 170, 585, 658
BseGI GGATG 3 cut(s) 34, 355, 612
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 567
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 3 cut(s) 353, 559, 650
BshFI GGCC 2 cut(s) 222, 663
BsiHKCI CYCGRG 2 cut(s) 46, 263
BsiSI CCGG 4 cut(s) 66, 78, 159, 219
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 381
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 381
BsnI GGCC 2 cut(s) 222, 663
BsoBI CYCGRG 2 cut(s) 46, 263
Bsp143I GATC 2 cut(s) 8, 52
BspACI CCGC 3 cut(s) 381, 399, 616
BspANI GGCC 2 cut(s) 222, 663
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 640
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 2 cut(s) 16, 60
BspQI GCTCTTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 4 cut(s) 47, 170, 585, 658
BssMI GATC 2 cut(s) 8, 52
BssNI GRCGYC 2 cut(s) 108, 117
BssT1I CCWWGG 1 cut(s) 658
Bst2UI CCWGG 3 cut(s) 148, 296, 586
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 2 cut(s) 108, 117
BstAPI GCANNNNNTGC 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 403, 437
BstEII GGTNACC 1 cut(s) 165
BstF5I GGATG 3 cut(s) 34, 355, 612
BstKTI GATC 2 cut(s) 11, 55
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 2 cut(s) 8, 52
BstMWI GCNNNNNNNGC 4 cut(s) 114, 350, 419, 578
BstNI CCWGG 3 cut(s) 148, 296, 586
BstPI GGTNACC 1 cut(s) 165
BstSCI CCNGG 6 cut(s) 64, 76, 146, 158, 294, 584
BstSFI CTRYAG 1 cut(s) 636
BstV1I GCAGC 3 cut(s) 353, 559, 650
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 2 cut(s) 222, 663
BtsCI GGATG 3 cut(s) 34, 355, 612
Cac8I GCNNGC 2 cut(s) 403, 437
CaiI CAGNNNCTG 1 cut(s) 17
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 395
CseI GACGC 3 cut(s) 97, 106, 610
Csp6I GTAC 3 cut(s) 40, 74, 644
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviQI GTAC 3 cut(s) 40, 74, 644
DpnI GATC 2 cut(s) 10, 54
DpnII GATC 2 cut(s) 8, 52
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 658
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 2 cut(s) 321, 440
Eco88I CYCGRG 2 cut(s) 46, 263
Eco91I GGTNACC 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 395
EcoO65I GGTNACC 1 cut(s) 165
EcoRII CCWGG 3 cut(s) 146, 294, 584
EcoT14I CCWWGG 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 658
Esp3I CGTCTC 1 cut(s) 124
FaqI GGGAC 1 cut(s) 381
FauI CCCGC 1 cut(s) 406
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 342, 573, 639
FokI GGATG 3 cut(s) 21, 362, 599
Fsp4HI GCNGC 3 cut(s) 342, 573, 639
FspBI CTAG 1 cut(s) 402
GluI GCNGC 3 cut(s) 342, 573, 639
HaeIII GGCC 2 cut(s) 222, 663
HapII CCGG 4 cut(s) 66, 78, 159, 219
HgaI GACGC 3 cut(s) 97, 106, 610
Hin1I GRCGYC 2 cut(s) 108, 117
HinfI GANTC 4 cut(s) 14, 31, 69, 329
HpaII CCGG 4 cut(s) 66, 78, 159, 219
HphI GGTGA 5 cut(s) 136, 159, 208, 338, 536
Hpy166II GTNNAC 2 cut(s) 96, 112
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 1 cut(s) 333
Hpy8I GTNNAC 2 cut(s) 96, 112
Hpy99I CGWCG 2 cut(s) 137, 604
HpyAV CCTTC 5 cut(s) 236, 365, 386, 416, 515
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4V TGCA 7 cut(s) 254, 341, 353, 413, 422, 575, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 350, 419, 578
Hsp92I GRCGYC 2 cut(s) 108, 117
Kzo9I GATC 2 cut(s) 8, 52
LguI GCTCTTC 1 cut(s) 158
Lsp1109I GCAGC 3 cut(s) 353, 559, 650
LweI GCATC 1 cut(s) 340
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 3 cut(s) 165, 290, 524
MalI GATC 2 cut(s) 10, 54
MboI GATC 2 cut(s) 8, 52
MboII GAAGA 3 cut(s) 145, 637, 640
MflI RGATCY 1 cut(s) 52
MluCI AATT 2 cut(s) 530, 541
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 7 cut(s) 42, 133, 152, 156, 165, 339, 592
MseI TTAA 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 4 cut(s) 66, 78, 159, 219
MspR9I CCNGG 6 cut(s) 66, 78, 148, 160, 296, 586
MvaI CCWGG 3 cut(s) 148, 296, 586
MwoI GCNNNNNNNGC 4 cut(s) 114, 350, 419, 578
NciI CCSGG 3 cut(s) 66, 78, 160
NdeII GATC 2 cut(s) 8, 52
NheI GCTAGC 1 cut(s) 401
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 165, 524
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PfeI GAWTC 3 cut(s) 31, 69, 329
Pfl23II CGTACG 1 cut(s) 39
PfoI TCCNGGA 1 cut(s) 158
PkrI GCNGC 3 cut(s) 343, 574, 640
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 3 cut(s) 146, 294, 584
PspEI GGTNACC 1 cut(s) 165
PspGI CCWGG 3 cut(s) 146, 294, 584
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 1 cut(s) 395
PspPPI RGGWCCY 1 cut(s) 395
PstI CTGCAG 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 3 cut(s) 41, 75, 645
RsaNI GTAC 3 cut(s) 40, 74, 644
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 3 cut(s) 342, 573, 639
Sau3AI GATC 2 cut(s) 8, 52
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 6 cut(s) 66, 78, 148, 160, 296, 586
SetI ASST 9 cut(s) 63, 149, 167, 297, 328, 397, 449, 483, 526
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 636
SinI GGWCC 1 cut(s) 395
Sse9I AATT 2 cut(s) 530, 541
SsiI CCGC 3 cut(s) 381, 399, 616
SspMI CTAG 1 cut(s) 402
StyD4I CCNGG 6 cut(s) 64, 76, 146, 158, 294, 584
StyI CCWWGG 1 cut(s) 658
TaaI ACNGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 240, 369
TasI AATT 2 cut(s) 530, 541
TatI WGTACW 1 cut(s) 643
TfiI GAWTC 3 cut(s) 31, 69, 329
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TseFI GTSAC 2 cut(s) 165, 524
TseI GCWGC 3 cut(s) 341, 572, 638
Tsp45I GTSAC 2 cut(s) 165, 524
TspDTI ATGAA 1 cut(s) 493
TspGWI ACGGA 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 2 cut(s) 530, 541
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.