AT1G20220

Alba

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004900
Physical Location & Seq
Reverse (-)
7004824 .. 7007717
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20220.1

Sequence Viewer

Length: 948 bp
ATGGATAAGTATCAGAGAGTTGAGAAGCCGAAGGCGGATACGCCGATTGCTGAGAATGAGATTCGTATCACCAGTATGGGTAGGGCACGGAACTACATCACCTATGCGATGGCTCTTCTTCAGGAAAACAAATCGAATGAAGTTATCTTCAAGGCTATGGGAAGAGCTATCAACAAGAGTGTGACCATAGTTGAGCTGATTAAGAGAAGGATTCCTGGTCTTCATCAGATCACATCTATTGGATCAACGGACATTACAGATACCTGGGAACCTACAGAGGAAGGCCTTCAAACAATTGAGACCACGAGGCATGTGTCGATGATAACCATAACACTTTCCAAGGAGCAGTTGAACACATCCTCAGTTGGGTACCAGTGTCCGATTCCTATTGAGATGGTGAAGCCATTGGCTGAGATTGACTACGAAGGGCAAGACGGATCTCCTAGAGGCAGAGGAGGAAGAAGAGGTAGAGGAGGAAGAGGAAGGGGAAGGGGAAGAGGTGGTAGAGGAAATGGACCTGCGAATGTTGAGTATGATGATGGAGGAAGGGGAAGAGGTGGCAGAGGAAACGGATATGTGAACAATGAGTATGATGATGGAGGAAGGGGAAGAGGCGGCAGAGGAAGTGGATATGTGAACAATGAGTATAATGATGGAGGTATGGAGCAGGACCGGTCATATGGCAGAGGACGCGGGCGTGGGAGAGGAGGTGGACGTGGTGGTCGAGGAAGAGGAGGTTACAATGGCCCCCCACCACCTTACTATGAAGCTCAACAAGATGGAGGAGACTATGGATACAACAATGTTGCTCCTCCAGCAGACCATGGATATGATGGTCCTCCGCCTCAGGGACGTGGACGTGGCCGTGGAAGGGGAGGTCGTGGAAGAGGAGGAGGCCGTGGTGGATTCAACAGATCAAATGGAGCACCTATCCAGGCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko03013 map03008 map03013
Pfam Domains
Protein Families
Alba

Protein Analysis

315

Amino Acids

33.76

Weight (kDa)

9.92

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 19 - 83 6.7e-20 Alba
Rpp20 PF12328 41 - 110 1e-05 Rpp20 subunit of nuclear RNase MRP and P
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 720
Acc36I ACCTGC 1 cut(s) 526
Acc65I GGTACC 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 369
AccII CGCG 1 cut(s) 693
AciI CCGC 4 cut(s) 35, 615, 693, 842
AclWI GGATC 2 cut(s) 250, 445
AcoI YGGCCR 1 cut(s) 862
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 3 cut(s) 366, 848, 871
AgeI ACCGGT 1 cut(s) 672
AgsI TTSAA 4 cut(s) 151, 290, 352, 910
AjiI CACGTC 3 cut(s) 716, 854, 860
AjnI CCWGG 3 cut(s) 214, 263, 933
AluBI AGCT 3 cut(s) 167, 196, 770
AluI AGCT 3 cut(s) 167, 196, 770
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 2 cut(s) 293, 780
AlwI GGATC 2 cut(s) 250, 445
AoxI GGCC 4 cut(s) 283, 745, 862, 895
ApeKI GCWGC 2 cut(s) 938, 941
AsiGI ACCGGT 1 cut(s) 672
Asp700I GAANNNNTTC 1 cut(s) 285
Asp718I GGTACC 1 cut(s) 369
AspS9I GGNCC 4 cut(s) 515, 670, 746, 836
AsuHPI GGTGA 3 cut(s) 61, 91, 409
AvaII GGWCC 3 cut(s) 515, 670, 836
AxyI CCTNAGG 1 cut(s) 846
BaeGI GKGCMC 1 cut(s) 88
BaeI ACNNNNGTAYC 2 cut(s) 361, 394
BanI GGYRCC 1 cut(s) 369
BauI CACGAG 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 2 cut(s) 925, 928
BccI CCATC 7 cut(s) 103, 388, 533, 590, 647, 773, 827
BceAI ACGGC 2 cut(s) 849, 882
BciT130I CCWGG 3 cut(s) 216, 265, 935
BciVI GTATCC 2 cut(s) 31, 788
BcoDI GTCTC 2 cut(s) 293, 780
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 273
BfuAI ACCTGC 1 cut(s) 526
BfuI GTATCC 2 cut(s) 31, 788
BisI GCNGC 3 cut(s) 616, 939, 942
BlsI GCNGC 3 cut(s) 617, 940, 943
Bme1390I CCNGG 3 cut(s) 216, 265, 935
Bme18I GGWCC 3 cut(s) 515, 670, 836
BmgBI CACGTC 3 cut(s) 716, 854, 860
BmgT120I GGNCC 4 cut(s) 515, 670, 746, 836
BmiI GGNNCC 3 cut(s) 270, 371, 748
BmrFI CCNGG 3 cut(s) 216, 265, 935
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 798
BsaBI GATNNNNATC 2 cut(s) 9, 65
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 264, 339, 823, 865, 898
BsaWI WCCGGW 1 cut(s) 672
BsaXI ACNNNNNCTCC 2 cut(s) 694, 724
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 848, 871
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 2 cut(s) 72, 373
Bse21I CCTNAGG 1 cut(s) 846
Bse8I GATNNNNATC 2 cut(s) 9, 65
BseBI CCWGG 3 cut(s) 216, 265, 935
BseDI CCNNGG 5 cut(s) 264, 339, 823, 865, 898
BseGI GGATG 1 cut(s) 356
BseJI GATNNNNATC 2 cut(s) 9, 65
BseLI CCNNNNNNNGG 3 cut(s) 366, 848, 871
BseMII CTCAG 4 cut(s) 42, 375, 402, 860
BseNI ACTGG 2 cut(s) 72, 373
BseRI GAGGAG 8 cut(s) 468, 486, 720, 747, 798, 801, 903, 906
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 2 cut(s) 925, 928
Bsh1236I CGCG 1 cut(s) 693
BshFI GGCC 4 cut(s) 285, 747, 864, 897
BshNI GGYRCC 1 cut(s) 369
BshTI ACCGGT 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 1 cut(s) 673
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 3 cut(s) 366, 848, 871
BsmAI GTCTC 2 cut(s) 293, 780
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 4 cut(s) 285, 747, 864, 897
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 88, 928
Bsp143I GATC 4 cut(s) 228, 242, 437, 914
Bsp19I CCATGG 1 cut(s) 823
BspACI CCGC 4 cut(s) 35, 615, 693, 842
BspANI GGCC 4 cut(s) 285, 747, 864, 897
BspCNI CTCAG 4 cut(s) 43, 374, 403, 859
BspFNI CGCG 1 cut(s) 693
BspLI GGNNCC 3 cut(s) 270, 371, 748
BspMI ACCTGC 1 cut(s) 526
BspPI GGATC 2 cut(s) 250, 445
BspQI GCTCTTC 2 cut(s) 120, 157
BspT107I GGYRCC 1 cut(s) 369
BspTNI GGTCTC 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 672
BsrI ACTGG 2 cut(s) 72, 373
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 5 cut(s) 264, 339, 823, 865, 898
BssMI GATC 4 cut(s) 228, 242, 437, 914
BssSI CACGAG 1 cut(s) 304
BssT1I CCWWGG 2 cut(s) 339, 823
Bst2BI CACGAG 1 cut(s) 304
Bst2UI CCWGG 3 cut(s) 216, 265, 935
Bst6I CTCTTC 9 cut(s) 120, 157, 457, 472, 490, 547, 604, 724, 880
BstC8I GCNNGC 1 cut(s) 695
BstDEI CTNAG 4 cut(s) 51, 361, 411, 846
BstDSI CCRYGG 3 cut(s) 823, 865, 898
BstF5I GGATG 1 cut(s) 356
BstFNI CGCG 1 cut(s) 693
BstKTI GATC 4 cut(s) 231, 245, 440, 917
BstMAI GTCTC 2 cut(s) 293, 780
BstMBI GATC 4 cut(s) 228, 242, 437, 914
BstMWI GCNNNNNNNGC 2 cut(s) 690, 815
BstNI CCWGG 3 cut(s) 216, 265, 935
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 3 cut(s) 214, 263, 933
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 2 cut(s) 925, 928
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 437
BstYI RGATCY 1 cut(s) 437
Bsu36I CCTNAGG 1 cut(s) 846
BsuI GTATCC 2 cut(s) 31, 788
BsuRI GGCC 4 cut(s) 285, 747, 864, 897
BtgI CCRYGG 3 cut(s) 823, 865, 898
BtgZI GCGATG 1 cut(s) 122
BtrI CACGTC 3 cut(s) 716, 854, 860
BtsCI GGATG 1 cut(s) 356
BtsIMutI CAGTG 1 cut(s) 380
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 695
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 4 cut(s) 515, 670, 746, 836
CseI GACGC 1 cut(s) 699
Csp6I GTAC 1 cut(s) 370
CspAI ACCGGT 1 cut(s) 672
CviAII CATG 3 cut(s) 311, 824, 945
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 4 cut(s) 51, 361, 411, 846
DpnI GATC 4 cut(s) 230, 244, 439, 916
DpnII GATC 4 cut(s) 228, 242, 437, 914
DrdI GACNNNNNNGTC 1 cut(s) 720
DseDI GACNNNNNNGTC 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 9 cut(s) 120, 157, 457, 472, 490, 547, 604, 724, 880
EarI CTCTTC 9 cut(s) 120, 157, 457, 472, 490, 547, 604, 724, 880
EciI GGCGGA 2 cut(s) 50, 831
Eco130I CCWWGG 2 cut(s) 339, 823
Eco147I AGGCCT 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 3 cut(s) 515, 670, 836
Eco57I CTGAAG 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 846
EcoRII CCWGG 3 cut(s) 214, 263, 933
EcoT14I CCWWGG 2 cut(s) 339, 823
ErhI CCWWGG 2 cut(s) 339, 823
FaeI CATG 3 cut(s) 314, 827, 948
FaqI GGGAC 1 cut(s) 864
FatI CATG 3 cut(s) 310, 823, 944
FauI CCCGC 1 cut(s) 686
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 616, 939, 942
FokI GGATG 1 cut(s) 343
Fsp4HI GCNGC 3 cut(s) 616, 939, 942
FspBI CTAG 1 cut(s) 444
GluI GCNGC 3 cut(s) 616, 939, 942
GsuI CTGGAG 1 cut(s) 798
HaeIII GGCC 4 cut(s) 285, 747, 864, 897
HapII CCGG 1 cut(s) 673
HgaI GACGC 1 cut(s) 699
Hin1II CATG 3 cut(s) 314, 827, 948
HinfI GANTC 4 cut(s) 61, 211, 382, 906
HpaII CCGG 1 cut(s) 673
HphI GGTGA 3 cut(s) 61, 91, 409
Hpy166II GTNNAC 4 cut(s) 580, 637, 713, 857
Hpy188I TCNGA 3 cut(s) 15, 228, 381
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 4 cut(s) 580, 637, 713, 857
HpyCH4IV ACGT 3 cut(s) 715, 853, 859
HpyCH4V TGCA 1 cut(s) 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 690, 815
HpyF3I CTNAG 4 cut(s) 51, 361, 411, 846
HpySE526I ACGT 3 cut(s) 715, 853, 859
Hsp92II CATG 3 cut(s) 314, 827, 948
KpnI GGTACC 1 cut(s) 373
Kzo9I GATC 4 cut(s) 228, 242, 437, 914
LguI GCTCTTC 2 cut(s) 120, 157
LmnI GCTCC 4 cut(s) 343, 664, 814, 923
Lsp1109I GCAGC 2 cut(s) 925, 928
MaeI CTAG 1 cut(s) 444
MaeII ACGT 3 cut(s) 715, 853, 859
MaeIII GTNAC 2 cut(s) 181, 737
MalI GATC 4 cut(s) 230, 244, 439, 916
MboI GATC 4 cut(s) 228, 242, 437, 914
MfeI CAATTG 1 cut(s) 294
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 2 cut(s) 88, 928
MluCI AATT 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 2 cut(s) 74, 828
MspI CCGG 1 cut(s) 673
MspR9I CCNGG 3 cut(s) 216, 265, 935
MunI CAATTG 1 cut(s) 294
MvaI CCWGG 3 cut(s) 216, 265, 935
MvnI CGCG 1 cut(s) 693
MwoI GCNNNNNNNGC 2 cut(s) 690, 815
NcoI CCATGG 1 cut(s) 823
NdeI CATATG 1 cut(s) 679
NdeII GATC 4 cut(s) 228, 242, 437, 914
NlaIII CATG 3 cut(s) 314, 827, 948
NlaIV GGNNCC 3 cut(s) 270, 371, 748
NmuCI GTSAC 1 cut(s) 181
NspI RCATGY 1 cut(s) 314
PceI AGGCCT 1 cut(s) 285
PciSI GCTCTTC 2 cut(s) 120, 157
PcsI WCGNNNNNNNCGW 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 4 cut(s) 61, 211, 382, 906
PinAI ACCGGT 1 cut(s) 672
PkrI GCNGC 3 cut(s) 617, 940, 943
Psp6I CCWGG 3 cut(s) 214, 263, 933
PspGI CCWGG 3 cut(s) 214, 263, 933
PspN4I GGNNCC 3 cut(s) 270, 371, 748
PspPI GGNCC 4 cut(s) 515, 670, 746, 836
PsuI RGATCY 1 cut(s) 437
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 2 cut(s) 74, 828
SapI GCTCTTC 2 cut(s) 120, 157
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 3 cut(s) 616, 939, 942
Sau3AI GATC 4 cut(s) 228, 242, 437, 914
Sau96I GGNCC 4 cut(s) 515, 670, 746, 836
ScrFI CCNGG 3 cut(s) 216, 265, 935
SduI GDGCHC 2 cut(s) 88, 928
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 3 cut(s) 515, 670, 836
SmiMI CAYNNNNRTG 2 cut(s) 74, 828
Sse9I AATT 1 cut(s) 294
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 4 cut(s) 35, 615, 693, 842
SspMI CTAG 1 cut(s) 444
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 214, 263, 933
StyI CCWWGG 2 cut(s) 339, 823
TaiI ACGT 3 cut(s) 718, 856, 862
TaqI TCGA 3 cut(s) 134, 317, 724
TasI AATT 1 cut(s) 294
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 4 cut(s) 61, 211, 382, 906
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 1 cut(s) 380
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 2 cut(s) 938, 941
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 3 cut(s) 153, 212, 780
TspGWI ACGGA 4 cut(s) 103, 263, 450, 585
TspRI CASTG 1 cut(s) 380
VpaK11BI GGWCC 3 cut(s) 515, 670, 836
XceI RCATGY 1 cut(s) 314
XcmI CCANNNNNNNNNTGG 1 cut(s) 830
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.