AT1G20770

coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004414
Physical Location & Seq
Reverse (-)
7215010 .. 7216897
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20770.1

Sequence Viewer

Length: 648 bp
ATGGCGGAGATTTACGAGGAAGGAGATGAATCAGTGGATGTGAATAAAGAATCAGAAGTGAAAACTGAGCTGGGAAAAGGGGGAGGAGATGAGAATGTTTTGCAGTTTCTAGATTCGTTGGATGGATATTTAACGCTTATGGATTCTGTGAATTCTAAGCTTCGAGAGGGATGGTTTGATCTAGCTAGTGCTCGACATTCTATGGGAACTTTACGCATCAACAGCACTCTCTTAGATCTTAAGTATCATCCCGCTTCTTCAACATTGCAAGTGACTGACCAAGATGTTGAATCTTTGGGATCAGTACCTCATTTTGCTTTGTCCAAGTGGGCTTCCAAGGGCGGTAGCAGGAAAGGCAAAGACTTCTCTACTGATACAGATTCGGAGATAGGCTCACCTCTCAGCCCACAGCTACGACATCGAGGAGTTTCTGAAGAGAAACCATCAGCCAAGGATGAAACCGTTTTAGCAGCTGATGAAGAGATAAAGAAAGAACGGGCAAAATCTCTGTCAGTATTTGGAGGTCTAGTCTCTCCTAAGCTGCGTGGCGCTCAACAATCATTTGAAACAGCTCTTGAAACTCTGGTGGAAATAGCCAACATGCGTGCATCGATGATATCTGCCTTTGAGCGGATCACAGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

215

Amino Acids

23.5

Weight (kDa)

4.92

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma22_CCDC115 PF21730 30 - 190 1.2e-11 Vma22/CCDC115
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 631
AciI CCGC 4 cut(s) 5, 252, 342, 631
AclWI GGATC 2 cut(s) 307, 641
AcsI RAATTY 1 cut(s) 151
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 1 cut(s) 630
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 4 cut(s) 261, 290, 566, 578
AluBI AGCT 7 cut(s) 70, 160, 185, 412, 473, 541, 572
AluI AGCT 7 cut(s) 70, 160, 185, 412, 473, 541, 572
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 2 cut(s) 307, 641
ApeKI GCWGC 2 cut(s) 470, 541
ApoI RAATTY 1 cut(s) 151
ArsI GACNNNNNNTTYG 2 cut(s) 494, 526
AspLEI GCGC 1 cut(s) 551
AsuHPI GGTGA 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 482, 528
BccI CCATC 3 cut(s) 116, 165, 451
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 4 cut(s) 110, 182, 186, 527
BfoI RGCGCY 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 239
BglII AGATCT 1 cut(s) 235
BisI GCNGC 2 cut(s) 471, 542
BlsI GCNGC 2 cut(s) 472, 543
BmsI GCATC 2 cut(s) 225, 617
BplI GAGNNNNNCTC 2 cut(s) 377, 409
Bpu10I CCTNAGC 1 cut(s) 537
Bsa29I ATCGAT 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 336, 450
BsaXI ACNNNNNCTCC 2 cut(s) 513, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 263
BseCI ATCGAT 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 336, 450
BseGI GGATG 5 cut(s) 43, 127, 176, 247, 460
BseLI CCNNNNNNNGG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 57, 415
BseRI GAGGAG 2 cut(s) 99, 438
BseXI GCAGC 2 cut(s) 482, 528
BseYI CCCAGC 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 611
BsiHKAI GWGCWC 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 630
BsmAI GTCTC 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 4 cut(s) 178, 235, 299, 633
BspACI CCGC 4 cut(s) 5, 252, 342, 631
BspCNI CTCAG 2 cut(s) 58, 414
BspDI ATCGAT 1 cut(s) 611
BspPI GGATC 2 cut(s) 307, 641
BspTI CTTAAG 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 631
BsrDI GCAATG 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 336, 450
BssMI GATC 4 cut(s) 178, 235, 299, 633
BssT1I CCWWGG 2 cut(s) 336, 450
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 2 cut(s) 429, 474
BstAFI CTTAAG 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 5 cut(s) 66, 156, 232, 401, 537
BstF5I GGATG 5 cut(s) 43, 127, 176, 247, 460
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 4 cut(s) 181, 238, 302, 636
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 4 cut(s) 178, 235, 299, 633
BstMWI GCNNNNNNNGC 2 cut(s) 222, 354
BstNSI RCATGY 1 cut(s) 604
BstV1I GCAGC 2 cut(s) 482, 528
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 611
BsuTUI ATCGAT 1 cut(s) 611
BtsCI GGATG 5 cut(s) 43, 127, 176, 247, 460
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 606
CfoI GCGC 1 cut(s) 551
ClaI ATCGAT 1 cut(s) 611
Csp6I GTAC 1 cut(s) 305
CviAII CATG 1 cut(s) 601
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 5 cut(s) 66, 156, 232, 401, 537
DpnI GATC 4 cut(s) 180, 237, 301, 635
DpnII GATC 4 cut(s) 178, 235, 299, 633
Eam1104I CTCTTC 2 cut(s) 429, 474
EarI CTCTTC 2 cut(s) 429, 474
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 336, 450
Eco32I GATATC 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 151
EcoRV GATATC 1 cut(s) 618
EcoT14I CCWWGG 2 cut(s) 336, 450
ErhI CCWWGG 2 cut(s) 336, 450
FaeI CATG 1 cut(s) 604
FaiI YATR 3 cut(s) 140, 203, 602
FatI CATG 1 cut(s) 600
FauI CCCGC 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 471, 542
FokI GGATG 5 cut(s) 50, 134, 183, 234, 467
Fsp4HI GCNGC 2 cut(s) 471, 542
FspBI CTAG 4 cut(s) 110, 182, 186, 527
GlaI GCGC 1 cut(s) 550
GluI GCNGC 2 cut(s) 471, 542
GsaI CCCAGC 1 cut(s) 74
HaeII RGCGCY 1 cut(s) 552
HhaI GCGC 1 cut(s) 551
Hin1II CATG 1 cut(s) 604
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 6 cut(s) 29, 50, 113, 143, 290, 380
HphI GGTGA 1 cut(s) 387
Hpy188I TCNGA 3 cut(s) 55, 385, 433
Hpy188III TCNNGA 3 cut(s) 110, 164, 575
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 3 cut(s) 103, 268, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 222, 354
HpyF3I CTNAG 5 cut(s) 66, 156, 232, 401, 537
Hsp92II CATG 1 cut(s) 604
HspAI GCGC 1 cut(s) 549
Kzo9I GATC 4 cut(s) 178, 235, 299, 633
LpnPI CCDG 3 cut(s) 56, 334, 569
Lsp1109I GCAGC 2 cut(s) 482, 528
LweI GCATC 2 cut(s) 225, 617
MaeI CTAG 4 cut(s) 110, 182, 186, 527
MaeIII GTNAC 1 cut(s) 271
MalI GATC 4 cut(s) 180, 237, 301, 635
MbiI CCGCTC 1 cut(s) 631
MboI GATC 4 cut(s) 178, 235, 299, 633
MboII GAAGA 3 cut(s) 249, 446, 491
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 1 cut(s) 151
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 7 cut(s) 10, 77, 160, 318, 408, 416, 515
MseI TTAA 2 cut(s) 131, 240
MspA1I CMGCKG 1 cut(s) 473
MspCI CTTAAG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 222, 354
NdeII GATC 4 cut(s) 178, 235, 299, 633
NlaIII CATG 1 cut(s) 604
NmuCI GTSAC 1 cut(s) 271
NspI RCATGY 1 cut(s) 604
PfeI GAWTC 6 cut(s) 29, 50, 113, 143, 290, 380
PkrI GCNGC 2 cut(s) 472, 543
PspFI CCCAGC 1 cut(s) 70
PsuI RGATCY 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SaqAI TTAA 2 cut(s) 131, 240
SatI GCNGC 2 cut(s) 471, 542
Sau3AI GATC 4 cut(s) 178, 235, 299, 633
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 2 cut(s) 225, 617
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 151
SsiI CCGC 4 cut(s) 5, 252, 342, 631
SspMI CTAG 4 cut(s) 110, 182, 186, 527
StyI CCWWGG 2 cut(s) 336, 450
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 4 cut(s) 163, 193, 421, 611
TasI AATT 1 cut(s) 151
TfiI GAWTC 6 cut(s) 29, 50, 113, 143, 290, 380
Tru1I TTAA 2 cut(s) 131, 240
Tru9I TTAA 2 cut(s) 131, 240
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 2 cut(s) 470, 541
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 3 cut(s) 42, 471, 492
TspRI CASTG 1 cut(s) 39
Vha464I CTTAAG 1 cut(s) 239
XapI RAATTY 1 cut(s) 151
XbaI TCTAGA 1 cut(s) 109
XceI RCATGY 1 cut(s) 604
XspI CTAG 4 cut(s) 110, 182, 186, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.