Rh1BG187500

coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004414
Physical Location & Seq
Reverse (-)
30222279 .. 30226813
4535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG187500.1

Sequence Viewer

Length: 924 bp
ATGGAAGCACAGAGCTTGAAAGAGGACAATCCTAAATTGCAGAGCTTGGAAGAAGACCCTAAATCAAAGGGCTTGGATGAAGAAGATACTAAATTACAGAGCTTGAAAGAAGACGGTTCTAAATCACTAGAAGACCCTAAATCACAGAGCTTGAAAGAAGAAGACCATAAATCACAGAGATTGAAAGAAGAGGAATGTAAGTCGCACAGCTCAGAAGAAGACCTTAAGTTAAACAGCTTGGAAGAAGACCCTAAATCACAGAGCTTGAAAGAAGAAGACCCTAAATTACAGAGCTTGGAAGAGGAAGAGCCTAAATCACAGAGCTTGAAAGAAGAAGGACAAGATAGCTTGGGAGACCCTAGATTCAATGAAAATGCATTGCAATTCCTAGATTCAATGGATGGCTATTTAACACTATTGGATTCTTTGTCTTGGACACTTCGGCAGGGATGGCTACAACTAGCGAGTGCGCGCCATTCAATGGGTGGGTCGCGGGTGAGCAGTGCTGTGTTGGATATGAAGCCCCACTCGGCTGCTACTTTTTTGCAAGTCAGCAAACAATCCAATCCAGGAGCAGGGTTAGGGGAAATTCATCATTTTACATTGCTGAAATGGGCCTCCTCTGATAATGGAAATGGAGCTCCACATAGAGATGAGAGTTCGCAGCTAAGACAAAGAAAGGACAAGGATGGAGATTCAACTCCGGTTACAGCTGAAGATGATTATCAACATCAACTTCAGAAGGAGCGGTTTAAATCACTTTCGGTGTTTGGCGCTTTGGTTTCCCCCAAGCTACGGGCGGCTCAGCTCTCATTCGAAACTGCACTCGAAGCACTTGTAGAGATTGCTAACATGCGATCGTTGATGCTTTCCAATTTTCATGAAGTCAGAAAAGAAATGGAATCAGCAAATGGCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.41

Weight (kDa)

4.97

Isoelectric Point (pI)

62.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma22_CCDC115 PF21730 124 - 274 1.4e-10 Vma22/CCDC115
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 748
AccII CGCG 2 cut(s) 472, 493
AciI CCGC 3 cut(s) 493, 748, 800
AcsI RAATTY 1 cut(s) 588
AcuI CTGAAG 2 cut(s) 722, 735
AfiI CCNNNNNNNGG 3 cut(s) 481, 575, 795
AflII CTTAAG 1 cut(s) 224
AjnI CCWGG 1 cut(s) 568
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 1 cut(s) 348
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 2 cut(s) 533, 664
ApoI RAATTY 1 cut(s) 588
AspLEI GCGC 3 cut(s) 472, 474, 776
AspS9I GGNCC 1 cut(s) 615
AsuHPI GGTGA 1 cut(s) 508
AsuII TTCGAA 1 cut(s) 816
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 7 cut(s) 60, 117, 138, 168, 225, 252, 282
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 2 cut(s) 520, 676
BccI CCATC 3 cut(s) 395, 444, 683
BciT130I CCWGG 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 4 cut(s) 128, 360, 389, 461
BfoI RGCGCY 1 cut(s) 777
BfrI CTTAAG 1 cut(s) 224
BisI GCNGC 3 cut(s) 534, 665, 801
BlpI GCTNAGC 1 cut(s) 804
BlsI GCNGC 3 cut(s) 535, 666, 802
Bme1390I CCNGG 1 cut(s) 570
BmgT120I GGNCC 1 cut(s) 615
BmrFI CCNGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 855
BpiI GAAGAC 7 cut(s) 60, 117, 138, 168, 225, 252, 282
Bpu1102I GCTNAGC 1 cut(s) 804
Bpu14I TTCGAA 1 cut(s) 816
BsaBI GATNNNNATC 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 348
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 3 cut(s) 481, 575, 795
Bse3DI GCAATG 2 cut(s) 377, 602
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 1 cut(s) 570
BseGI GGATG 4 cut(s) 82, 406, 455, 694
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 481, 575, 795
BseMI GCAATG 2 cut(s) 377, 602
BseMII CTCAG 2 cut(s) 225, 818
BsePI GCGCGC 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 610
BseXI GCAGC 2 cut(s) 520, 676
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 2 cut(s) 472, 493
Bsh1285I CGRYCG 1 cut(s) 860
BshFI GGCC 1 cut(s) 617
BsiEI CGRYCG 1 cut(s) 860
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 1 cut(s) 704
BslI CCNNNNNNNGG 3 cut(s) 481, 575, 795
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 1 cut(s) 617
Bso31I GGTCTC 1 cut(s) 348
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 1 cut(s) 857
Bsp1720I GCTNAGC 1 cut(s) 804
BspACI CCGC 3 cut(s) 493, 748, 800
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 2 cut(s) 224, 817
BspFNI CGCG 2 cut(s) 472, 493
BspHI TCATGA 1 cut(s) 880
BspQI GCTCTTC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 816
BspTI CTTAAG 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 348
BsrBI CCGCTC 1 cut(s) 748
BsrDI GCAATG 2 cut(s) 377, 602
BssHII GCGCGC 1 cut(s) 470
BssMI GATC 1 cut(s) 857
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 3 cut(s) 183, 294, 300
BstAFI CTTAAG 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 472
BstDEI CTNAG 3 cut(s) 211, 668, 804
BstF5I GGATG 4 cut(s) 82, 406, 455, 694
BstFNI CGCG 2 cut(s) 472, 493
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 3 cut(s) 472, 474, 776
BstKTI GATC 1 cut(s) 860
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 1 cut(s) 857
BstMCI CGRYCG 1 cut(s) 860
BstMWI GCNNNNNNNGC 2 cut(s) 451, 830
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 1 cut(s) 856
BstSCI CCNGG 1 cut(s) 568
BstUI CGCG 2 cut(s) 472, 493
BstV1I GCAGC 2 cut(s) 520, 676
BstV2I GAAGAC 7 cut(s) 60, 117, 138, 168, 225, 252, 282
BsuRI GGCC 1 cut(s) 617
BtsCI GGATG 4 cut(s) 82, 406, 455, 694
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 1 cut(s) 508
Cac8I GCNNGC 1 cut(s) 472
CciI TCATGA 1 cut(s) 880
CfoI GCGC 3 cut(s) 472, 474, 776
Cfr13I GGNCC 1 cut(s) 615
CviAII CATG 2 cut(s) 853, 881
DdeI CTNAG 3 cut(s) 211, 668, 804
DpnI GATC 1 cut(s) 859
DpnII GATC 1 cut(s) 857
DraI TTTAAA 1 cut(s) 754
Eam1104I CTCTTC 3 cut(s) 183, 294, 300
EarI CTCTTC 3 cut(s) 183, 294, 300
Ecl136II GAGCTC 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 643
Eco31I GGTCTC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 641
Eco57I CTGAAG 2 cut(s) 722, 735
EcoICRI GAGCTC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 568
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 643
FaeI CATG 2 cut(s) 856, 884
FaiI YATR 5 cut(s) 168, 518, 648, 854, 882
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 2 cut(s) 852, 880
FauI CCCGC 1 cut(s) 486
Fnu4HI GCNGC 3 cut(s) 534, 665, 801
FokI GGATG 4 cut(s) 89, 413, 462, 701
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 3 cut(s) 534, 665, 801
FspBI CTAG 4 cut(s) 128, 360, 389, 461
GlaI GCGC 3 cut(s) 471, 473, 775
GluI GCNGC 3 cut(s) 534, 665, 801
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 1 cut(s) 617
HapII CCGG 1 cut(s) 704
HhaI GCGC 3 cut(s) 472, 474, 776
Hin1II CATG 2 cut(s) 856, 884
Hin6I GCGC 3 cut(s) 470, 472, 774
HinP1I GCGC 3 cut(s) 470, 472, 774
HinfI GANTC 5 cut(s) 363, 392, 422, 695, 902
HpaII CCGG 1 cut(s) 704
HphI GGTGA 1 cut(s) 508
Hpy188I TCNGA 4 cut(s) 214, 625, 741, 890
Hpy188III TCNNGA 1 cut(s) 881
HpyAV CCTTC 2 cut(s) 329, 736
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4V TGCA 5 cut(s) 40, 377, 382, 547, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 451, 830
HpyF3I CTNAG 3 cut(s) 211, 668, 804
Hsp92II CATG 2 cut(s) 856, 884
HspAI GCGC 3 cut(s) 470, 472, 774
Kzo9I GATC 1 cut(s) 857
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 4 cut(s) 572, 638, 646, 745
LpnPI CCDG 5 cut(s) 431, 555, 561, 582, 717
Lsp1109I GCAGC 2 cut(s) 520, 676
LweI GCATC 1 cut(s) 855
MaeI CTAG 4 cut(s) 128, 360, 389, 461
MaeIII GTNAC 1 cut(s) 706
MalI GATC 1 cut(s) 859
MbiI CCGCTC 1 cut(s) 748
MboI GATC 1 cut(s) 857
MhlI GDGCHC 1 cut(s) 643
MluCI AATT 6 cut(s) 35, 92, 284, 383, 588, 874
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 5 cut(s) 16, 184, 295, 628, 631
Mph1103I ATGCAT 1 cut(s) 379
MseI TTAA 4 cut(s) 225, 230, 410, 753
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 713
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 2 cut(s) 472, 493
MwoI GCNNNNNNNGC 2 cut(s) 451, 830
NdeII GATC 1 cut(s) 857
NlaIII CATG 2 cut(s) 856, 884
NmeAIII GCCGAG 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 1 cut(s) 856
NspV TTCGAA 1 cut(s) 816
PagI TCATGA 1 cut(s) 880
PauI GCGCGC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 300
PfeI GAWTC 5 cut(s) 363, 392, 422, 695, 902
PflMI CCANNNNNTGG 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 3 cut(s) 535, 666, 802
Ple19I CGATCG 1 cut(s) 860
Psp124BI GAGCTC 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspPI GGNCC 1 cut(s) 615
PteI GCGCGC 1 cut(s) 470
PvuI CGATCG 1 cut(s) 860
PvuII CAGCTG 1 cut(s) 713
RseI CAYNNNNRTG 1 cut(s) 651
SacI GAGCTC 1 cut(s) 643
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 4 cut(s) 225, 230, 410, 753
SatI GCNGC 3 cut(s) 534, 665, 801
Sau3AI GATC 1 cut(s) 857
Sau96I GGNCC 1 cut(s) 615
ScrFI CCNGG 1 cut(s) 570
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 1 cut(s) 855
SfuI TTCGAA 1 cut(s) 816
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 6 cut(s) 35, 92, 284, 383, 588, 874
SsiI CCGC 3 cut(s) 493, 748, 800
SspMI CTAG 4 cut(s) 128, 360, 389, 461
SstI GAGCTC 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 568
TaaI ACNGT 1 cut(s) 116
TaqI TCGA 2 cut(s) 816, 828
TasI AATT 6 cut(s) 35, 92, 284, 383, 588, 874
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 5 cut(s) 363, 392, 422, 695, 902
Tru1I TTAA 4 cut(s) 225, 230, 410, 753
Tru9I TTAA 4 cut(s) 225, 230, 410, 753
TscAI CASTG 1 cut(s) 508
TseI GCWGC 2 cut(s) 533, 664
TspDTI ATGAA 6 cut(s) 93, 384, 533, 581, 869, 897
TspRI CASTG 1 cut(s) 508
Van91I CCANNNNNTGG 1 cut(s) 481
Vha464I CTTAAG 1 cut(s) 224
XapI RAATTY 1 cut(s) 588
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XspI CTAG 4 cut(s) 128, 360, 389, 461
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.