RLG00000028546

coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004414
Physical Location & Seq
Forward (+)
24190073 .. 24193253
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028546

Sequence Viewer

Length: 915 bp
ATGGAAGCACAGAGCTTGAAACAGGACAATCCTAAATTGCAGAGCTTGGAAGAAGACCCTAAACCAAAGAGCTTGGAAGAAGAAGATACTGAATTACAGAGCTTGAAAGAAGACGGTTCTAAATCACTAGAAGACCCTAAATCACAGAGCTTGAAAGAAGAGGAATGTAAGTCGCACAGCTTGGAAGAAGACCTTAAGTTAAACAGCTTGGAAGAAGAGCCTAAATTGCAGAGCTTGAAAGATAAATCGCAGAGCTTGAAAGAAGAAGACCCTAAATTACAGAGCTTGGAAGAGGAAGAGCCTAAATCACAGAGCTTGAAAGAAGAAGTACAAGATAGCTTGGGAGACCCTAGATTCAATGAAAATGCATTGCAATTCCTAGATTCAATGGATGGGTATTTAACACTATTGGATTCATTGTCTTGGACGCTTCGGCAGGGATGGCTGCAACTAGCGAGTGCGCGCCATTCAATGGGTGGGTCACGGGTGAGCAGTGCTGTGTTGGATATGAAGCCCCACTCAGCTGCTACTTTTTTGCAAGTCAGCAAACAATCCAATCCAGGAGCAGGGTTAGGGGAAATTCATCATTTTACATTGCTGAAATGGGCCTCCTCTGATAATGGAAATGGAGCTCCACATAGAGATGCGAGTCCGCAGCTAAGACAAAGAAAGGACAAGGATGGAGATTCAGCTCCAGTTACTGCTGAAGATGATTATCAACATCAACTTCAGAAGGAGCGGTTTAAATCACTTTCGGTGTTTGGCGCTTTGGTTTCCCCCAAGCTACGGGCGGCTCAGCTCTCATTCGAAACTGCACTCGAAGCACTTGTAGAGATTGCTAACATGCGATCGTTGATGCTTTCCAATTTTCATGAACTCAGAAAAGAAATGGAATCAGCAAATGGCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

305

Amino Acids

34.04

Weight (kDa)

4.95

Isoelectric Point (pI)

66.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma22_CCDC115 PF21730 121 - 271 2.3e-09 Vma22/CCDC115
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 472
AccBSI CCGCTC 1 cut(s) 739
AccII CGCG 1 cut(s) 463
AciI CCGC 3 cut(s) 653, 739, 791
AcsI RAATTY 1 cut(s) 579
AcuI CTGAAG 2 cut(s) 713, 726
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 3 cut(s) 472, 566, 786
AflII CTTAAG 1 cut(s) 194
AgsI TTSAA 9 cut(s) 19, 106, 154, 238, 259, 319, 358, 387, 471
AjnI CCWGG 1 cut(s) 559
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 1 cut(s) 339
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 3 cut(s) 445, 524, 655
ApoI RAATTY 1 cut(s) 579
AspLEI GCGC 3 cut(s) 463, 465, 767
AspS9I GGNCC 1 cut(s) 606
AsuHPI GGTGA 1 cut(s) 499
AsuII TTCGAA 1 cut(s) 807
BanII GRGCYC 1 cut(s) 634
BbsI GAAGAC 5 cut(s) 60, 117, 138, 195, 273
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 3 cut(s) 432, 511, 667
BccI CCATC 3 cut(s) 386, 435, 674
BciT130I CCWGG 1 cut(s) 561
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 4 cut(s) 128, 351, 380, 452
BfoI RGCGCY 1 cut(s) 768
BfrI CTTAAG 1 cut(s) 194
BisI GCNGC 4 cut(s) 446, 525, 656, 792
BlpI GCTNAGC 1 cut(s) 795
BlsI GCNGC 4 cut(s) 447, 526, 657, 793
Bme1390I CCNGG 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 606
BmrFI CCNGG 1 cut(s) 561
BmsI GCATC 2 cut(s) 634, 846
BpiI GAAGAC 5 cut(s) 60, 117, 138, 195, 273
BpmI CTGGAG 1 cut(s) 678
Bpu1102I GCTNAGC 1 cut(s) 795
Bpu14I TTCGAA 1 cut(s) 807
BsaBI GATNNNNATC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 339
Bsc4I CCNNNNNNNGG 3 cut(s) 472, 566, 786
Bse1I ACTGG 1 cut(s) 695
Bse3DI GCAATG 2 cut(s) 368, 593
Bse8I GATNNNNATC 1 cut(s) 714
BseBI CCWGG 1 cut(s) 561
BseGI GGATG 3 cut(s) 397, 446, 685
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 3 cut(s) 472, 566, 786
BseMI GCAATG 2 cut(s) 368, 593
BseMII CTCAG 3 cut(s) 534, 809, 892
BseNI ACTGG 1 cut(s) 695
BsePI GCGCGC 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 601
BseXI GCAGC 3 cut(s) 432, 511, 667
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 1 cut(s) 463
Bsh1285I CGRYCG 1 cut(s) 851
BshFI GGCC 1 cut(s) 608
BsiEI CGRYCG 1 cut(s) 851
BsiHKAI GWGCWC 1 cut(s) 634
BslI CCNNNNNNNGG 3 cut(s) 472, 566, 786
BsmAI GTCTC 1 cut(s) 339
BsnI GGCC 1 cut(s) 608
Bso31I GGTCTC 1 cut(s) 339
Bsp119I TTCGAA 1 cut(s) 807
Bsp1286I GDGCHC 1 cut(s) 634
Bsp143I GATC 1 cut(s) 848
Bsp1720I GCTNAGC 1 cut(s) 795
BspACI CCGC 3 cut(s) 653, 739, 791
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 3 cut(s) 533, 808, 891
BspFNI CGCG 1 cut(s) 463
BspHI TCATGA 1 cut(s) 871
BspQI GCTCTTC 2 cut(s) 210, 291
BspT104I TTCGAA 1 cut(s) 807
BspTI CTTAAG 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 339
BsrBI CCGCTC 1 cut(s) 739
BsrDI GCAATG 2 cut(s) 368, 593
BsrI ACTGG 1 cut(s) 695
BssHII GCGCGC 1 cut(s) 461
BssMI GATC 1 cut(s) 848
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 4 cut(s) 153, 210, 285, 291
BstAFI CTTAAG 1 cut(s) 194
BstBI TTCGAA 1 cut(s) 807
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 4 cut(s) 520, 659, 795, 878
BstF5I GGATG 3 cut(s) 397, 446, 685
BstFNI CGCG 1 cut(s) 463
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 3 cut(s) 463, 465, 767
BstKTI GATC 1 cut(s) 851
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 1 cut(s) 848
BstMCI CGRYCG 1 cut(s) 851
BstMWI GCNNNNNNNGC 3 cut(s) 226, 442, 821
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 1 cut(s) 559
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 3 cut(s) 432, 511, 667
BstV2I GAAGAC 5 cut(s) 60, 117, 138, 195, 273
BsuRI GGCC 1 cut(s) 608
BtsCI GGATG 3 cut(s) 397, 446, 685
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 1 cut(s) 463
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 871
CfoI GCGC 3 cut(s) 463, 465, 767
Cfr13I GGNCC 1 cut(s) 606
CseI GACGC 1 cut(s) 436
Csp6I GTAC 1 cut(s) 329
CviAII CATG 2 cut(s) 844, 872
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 4 cut(s) 520, 659, 795, 878
DpnI GATC 1 cut(s) 850
DpnII GATC 1 cut(s) 848
DraI TTTAAA 1 cut(s) 745
Eam1104I CTCTTC 4 cut(s) 153, 210, 285, 291
EarI CTCTTC 4 cut(s) 153, 210, 285, 291
Ecl136II GAGCTC 1 cut(s) 632
Eco24I GRGCYC 1 cut(s) 634
Eco31I GGTCTC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 632
Eco57I CTGAAG 2 cut(s) 713, 726
EcoICRI GAGCTC 1 cut(s) 632
EcoRII CCWGG 1 cut(s) 559
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 634
FaeI CATG 2 cut(s) 847, 875
FaiI YATR 4 cut(s) 509, 639, 845, 873
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FatI CATG 2 cut(s) 843, 871
Fnu4HI GCNGC 4 cut(s) 446, 525, 656, 792
FokI GGATG 3 cut(s) 404, 453, 692
FriOI GRGCYC 1 cut(s) 634
Fsp4HI GCNGC 4 cut(s) 446, 525, 656, 792
FspBI CTAG 4 cut(s) 128, 351, 380, 452
GlaI GCGC 3 cut(s) 462, 464, 766
GluI GCNGC 4 cut(s) 446, 525, 656, 792
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 1 cut(s) 608
HgaI GACGC 1 cut(s) 436
HhaI GCGC 3 cut(s) 463, 465, 767
Hin1II CATG 2 cut(s) 847, 875
Hin6I GCGC 3 cut(s) 461, 463, 765
HinP1I GCGC 3 cut(s) 461, 463, 765
HinfI GANTC 6 cut(s) 354, 383, 413, 649, 686, 893
HphI GGTGA 1 cut(s) 499
Hpy188I TCNGA 3 cut(s) 616, 732, 881
Hpy188III TCNNGA 1 cut(s) 872
HpyAV CCTTC 1 cut(s) 727
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4V TGCA 7 cut(s) 40, 229, 368, 373, 448, 538, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 442, 821
HpyF3I CTNAG 4 cut(s) 520, 659, 795, 878
Hsp92II CATG 2 cut(s) 847, 875
HspAI GCGC 3 cut(s) 461, 463, 765
Kzo9I GATC 1 cut(s) 848
LguI GCTCTTC 2 cut(s) 210, 291
LmnI GCTCC 5 cut(s) 563, 629, 637, 697, 736
LpnPI CCDG 6 cut(s) 8, 422, 546, 552, 573, 708
Lsp1109I GCAGC 3 cut(s) 432, 511, 667
LweI GCATC 2 cut(s) 634, 846
MaeI CTAG 4 cut(s) 128, 351, 380, 452
MaeIII GTNAC 2 cut(s) 480, 697
MalI GATC 1 cut(s) 850
MbiI CCGCTC 1 cut(s) 739
MboI GATC 1 cut(s) 848
MhlI GDGCHC 1 cut(s) 634
MluCI AATT 7 cut(s) 35, 92, 224, 275, 374, 579, 865
MlyI GAGTC 1 cut(s) 658
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 4 cut(s) 154, 286, 619, 622
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 4 cut(s) 195, 200, 401, 744
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 561
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 3 cut(s) 226, 442, 821
NdeII GATC 1 cut(s) 848
NlaIII CATG 2 cut(s) 847, 875
NmuCI GTSAC 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 1 cut(s) 847
NspV TTCGAA 1 cut(s) 807
PagI TCATGA 1 cut(s) 871
PauI GCGCGC 1 cut(s) 461
PciSI GCTCTTC 2 cut(s) 210, 291
PfeI GAWTC 5 cut(s) 354, 383, 413, 686, 893
PflMI CCANNNNNTGG 1 cut(s) 472
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 4 cut(s) 447, 526, 657, 793
Ple19I CGATCG 1 cut(s) 851
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
Psp124BI GAGCTC 1 cut(s) 634
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspPI GGNCC 1 cut(s) 606
PstNI CAGNNNCTG 1 cut(s) 701
PteI GCGCGC 1 cut(s) 461
PvuI CGATCG 1 cut(s) 851
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 1 cut(s) 634
SapI GCTCTTC 2 cut(s) 210, 291
SaqAI TTAA 4 cut(s) 195, 200, 401, 744
SatI GCNGC 4 cut(s) 446, 525, 656, 792
Sau3AI GATC 1 cut(s) 848
Sau96I GGNCC 1 cut(s) 606
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 1 cut(s) 561
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 2 cut(s) 634, 846
SfuI TTCGAA 1 cut(s) 807
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 7 cut(s) 35, 92, 224, 275, 374, 579, 865
SsiI CCGC 3 cut(s) 653, 739, 791
SspMI CTAG 4 cut(s) 128, 351, 380, 452
SstI GAGCTC 1 cut(s) 634
StyD4I CCNGG 1 cut(s) 559
TaaI ACNGT 1 cut(s) 116
TaqI TCGA 2 cut(s) 807, 819
TasI AATT 7 cut(s) 35, 92, 224, 275, 374, 579, 865
TatI WGTACW 1 cut(s) 328
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 5 cut(s) 354, 383, 413, 686, 893
Tru1I TTAA 4 cut(s) 195, 200, 401, 744
Tru9I TTAA 4 cut(s) 195, 200, 401, 744
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 3 cut(s) 445, 524, 655
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 6 cut(s) 375, 405, 524, 572, 860, 888
TspRI CASTG 1 cut(s) 499
Van91I CCANNNNNTGG 1 cut(s) 472
Vha464I CTTAAG 1 cut(s) 194
XapI RAATTY 1 cut(s) 579
XceI RCATGY 1 cut(s) 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XspI CTAG 4 cut(s) 128, 351, 380, 452
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.