Rh7AG291400

coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004414
Physical Location & Seq
Forward (+)
31617939 .. 31620000
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG291400.1

Sequence Viewer

Length: 924 bp
ATGGAAGCACAGAGCTTGAAAGAGGCCAATCCTAGATTGCAGAGCTTGGAAGTAGACCCTAAATCAAAGGGCTTGGAAGAAGAAGATACTAAATTACAGAGCTTGAAAGAAGACGGTTCTAAATCACTAGAAGACCCTAAATCACAGAGCTTGAAAGAAGAAGACCATAAATCACAGCGATTGAAAGAAGAGGAATGTAAGTCGCACAGCTCGGAAGAAGACCTGAAGTTAAACAGCTCGGAAGAAGACCCTAAATCGCAGAGCTGGAAAGAAGAAGACCCTAAATTACAGAGCTTGGAAGAGGAAGAGCCTAAATCACAGAGCTTGAAAGAAGAAGGACGAGATAGCTTGGGAGACCCTAAATTCAATGAAAATGCATTGCAATTCCTGGATTCAATGGATGGGTATTTAACCCTAATGGATTCATTGTCTTGGACACTTCGGCAGGGATGGCTGCAGCTAGCGAGTGCGCGCCACTCAATGGGTGGGTCGCGGGTGAGCAGTGCGGTGTTGGATATGAAGCCCCACTCAGCTGCTACTTTTTTGCAAGTCAGCAAACAATCCAATCCACAAGTAGGGTTAGGGGAAATTCATCATTTTACATTGCTGAAATGGGCCTCATCGGATAATGGAAATGGAGCTCCACATAGAGATGTGAGTCCGCAGCTAAGACAAAGAAAGGACAAGGATGGAGATTCAGCCCCTGTTACAGCTGAAGATGACCATCATCAGCAACTTCAGAAGGAGCGGTTTAAATCACTTTCGGTGTTTGGCGCTTTGGTTTCCCCCAAGCCACGGGCGGCTCAGCTCTCATTCGAAACTGTGCTGGAAGCACTTGTAGAGATTGCTAACATGCAATCGTTGATGCTTTCCAATTTTCATGAAGTCAGAAAAGAAATGGAATCAGCAAATGGCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.45

Weight (kDa)

5.16

Isoelectric Point (pI)

63.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma22_CCDC115 PF21730 124 - 274 2.4e-07 Vma22/CCDC115
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 748
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 472, 493
AciI CCGC 5 cut(s) 493, 506, 662, 748, 800
AcsI RAATTY 2 cut(s) 362, 588
AcuI CTGAAG 3 cut(s) 245, 722, 735
AfiI CCNNNNNNNGG 3 cut(s) 481, 575, 795
AgsI TTSAA 7 cut(s) 19, 106, 154, 184, 328, 367, 396
AjnI CCWGG 1 cut(s) 387
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 1 cut(s) 348
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 2 cut(s) 24, 615
ApeKI GCWGC 4 cut(s) 454, 457, 533, 664
ApoI RAATTY 2 cut(s) 362, 588
AspLEI GCGC 3 cut(s) 472, 474, 776
AspS9I GGNCC 1 cut(s) 615
AsuHPI GGTGA 1 cut(s) 508
AsuII TTCGAA 1 cut(s) 816
AsuNHI GCTAGC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 6 cut(s) 117, 138, 168, 225, 252, 282
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 4 cut(s) 441, 469, 520, 676
BccI CCATC 4 cut(s) 395, 444, 683, 732
BciT130I CCWGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 3 cut(s) 33, 128, 461
BfmI CTRYAG 1 cut(s) 455
BfoI RGCGCY 1 cut(s) 777
BisI GCNGC 5 cut(s) 455, 458, 534, 665, 801
BlpI GCTNAGC 1 cut(s) 804
BlsI GCNGC 5 cut(s) 456, 459, 535, 666, 802
Bme1390I CCNGG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 615
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 855
BmtI GCTAGC 1 cut(s) 464
BpiI GAAGAC 6 cut(s) 117, 138, 168, 225, 252, 282
Bpu1102I GCTNAGC 1 cut(s) 804
Bpu14I TTCGAA 1 cut(s) 816
BsaBI GATNNNNATC 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 794
Bsc4I CCNNNNNNNGG 3 cut(s) 481, 575, 795
Bse3DI GCAATG 2 cut(s) 377, 602
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 794
BseGI GGATG 3 cut(s) 406, 455, 694
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 481, 575, 795
BseMI GCAATG 2 cut(s) 377, 602
BseMII CTCAG 2 cut(s) 543, 818
BsePI GCGCGC 1 cut(s) 470
BseXI GCAGC 4 cut(s) 441, 469, 520, 676
Bsh1236I CGCG 2 cut(s) 472, 493
BshFI GGCC 2 cut(s) 26, 617
BsiHKAI GWGCWC 1 cut(s) 643
BslI CCNNNNNNNGG 3 cut(s) 481, 575, 795
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 2 cut(s) 26, 617
Bso31I GGTCTC 1 cut(s) 348
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 1 cut(s) 643
Bsp1720I GCTNAGC 1 cut(s) 804
BspACI CCGC 5 cut(s) 493, 506, 662, 748, 800
BspANI GGCC 2 cut(s) 26, 617
BspCNI CTCAG 2 cut(s) 542, 817
BspFNI CGCG 2 cut(s) 472, 493
BspHI TCATGA 1 cut(s) 880
BspMAI CTGCAG 1 cut(s) 459
BspOI GCTAGC 1 cut(s) 464
BspQI GCTCTTC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 816
BspTNI GGTCTC 1 cut(s) 348
BsrBI CCGCTC 1 cut(s) 748
BsrDI GCAATG 2 cut(s) 377, 602
BssECI CCNNGG 1 cut(s) 794
BssHII GCGCGC 1 cut(s) 470
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 116, 823
Bst6I CTCTTC 3 cut(s) 183, 294, 300
BstBI TTCGAA 1 cut(s) 816
BstC8I GCNNGC 2 cut(s) 462, 472
BstDEI CTNAG 3 cut(s) 529, 668, 804
BstDSI CCRYGG 1 cut(s) 794
BstF5I GGATG 3 cut(s) 406, 455, 694
BstFNI CGCG 2 cut(s) 472, 493
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 3 cut(s) 472, 474, 776
BstMAI GTCTC 1 cut(s) 348
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 856
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 1 cut(s) 455
BstUI CGCG 2 cut(s) 472, 493
BstV1I GCAGC 4 cut(s) 441, 469, 520, 676
BstV2I GAAGAC 6 cut(s) 117, 138, 168, 225, 252, 282
BsuRI GGCC 2 cut(s) 26, 617
BtgI CCRYGG 1 cut(s) 794
BtsCI GGATG 3 cut(s) 406, 455, 694
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 1 cut(s) 508
Cac8I GCNNGC 2 cut(s) 462, 472
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 880
CfoI GCGC 3 cut(s) 472, 474, 776
Cfr13I GGNCC 1 cut(s) 615
CviAII CATG 2 cut(s) 853, 881
DdeI CTNAG 3 cut(s) 529, 668, 804
DraI TTTAAA 1 cut(s) 754
Eam1104I CTCTTC 3 cut(s) 183, 294, 300
EarI CTCTTC 3 cut(s) 183, 294, 300
Ecl136II GAGCTC 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 643
Eco31I GGTCTC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 641
Eco57I CTGAAG 3 cut(s) 245, 722, 735
EcoICRI GAGCTC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 643
FaeI CATG 2 cut(s) 856, 884
FaiI YATR 5 cut(s) 168, 518, 648, 854, 882
FatI CATG 2 cut(s) 852, 880
FauI CCCGC 1 cut(s) 486
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 455, 458, 534, 665, 801
FokI GGATG 3 cut(s) 413, 462, 701
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 5 cut(s) 455, 458, 534, 665, 801
FspBI CTAG 3 cut(s) 33, 128, 461
GlaI GCGC 3 cut(s) 471, 473, 775
GluI GCNGC 5 cut(s) 455, 458, 534, 665, 801
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 2 cut(s) 26, 617
HhaI GCGC 3 cut(s) 472, 474, 776
Hin1II CATG 2 cut(s) 856, 884
Hin6I GCGC 3 cut(s) 470, 472, 774
HinP1I GCGC 3 cut(s) 470, 472, 774
HinfI GANTC 5 cut(s) 392, 422, 658, 695, 902
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 5 cut(s) 214, 241, 625, 741, 890
Hpy188III TCNNGA 1 cut(s) 881
Hpy8I GTNNAC 1 cut(s) 55
HpyAV CCTTC 2 cut(s) 329, 736
HpyCH4III ACNGT 2 cut(s) 116, 823
HpyCH4V TGCA 6 cut(s) 40, 377, 382, 457, 547, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
HpyF3I CTNAG 3 cut(s) 529, 668, 804
Hsp92II CATG 2 cut(s) 856, 884
HspAI GCGC 3 cut(s) 470, 472, 774
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 3 cut(s) 638, 646, 745
LpnPI CCDG 7 cut(s) 236, 250, 374, 401, 431, 717, 812
Lsp1109I GCAGC 4 cut(s) 441, 469, 520, 676
LweI GCATC 1 cut(s) 855
MaeI CTAG 3 cut(s) 33, 128, 461
MaeIII GTNAC 1 cut(s) 706
MbiI CCGCTC 1 cut(s) 748
MhlI GDGCHC 1 cut(s) 643
MluCI AATT 6 cut(s) 92, 284, 362, 383, 588, 874
MlyI GAGTC 1 cut(s) 667
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 4 cut(s) 16, 184, 295, 628
Mph1103I ATGCAT 1 cut(s) 379
MseI TTAA 3 cut(s) 230, 410, 753
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 533, 713
MspR9I CCNGG 1 cut(s) 389
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 2 cut(s) 472, 493
MwoI GCNNNNNNNGC 1 cut(s) 451
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 2 cut(s) 856, 884
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 1 cut(s) 856
NspV TTCGAA 1 cut(s) 816
PagI TCATGA 1 cut(s) 880
PauI GCGCGC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 300
PfeI GAWTC 4 cut(s) 392, 422, 695, 902
PflMI CCANNNNNTGG 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 5 cut(s) 456, 459, 535, 666, 802
PleI GAGTC 1 cut(s) 666
PpsI GAGTC 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspPI GGNCC 1 cut(s) 615
PstI CTGCAG 1 cut(s) 459
PstNI CAGNNNCTG 1 cut(s) 704
PteI GCGCGC 1 cut(s) 470
PvuII CAGCTG 2 cut(s) 533, 713
RseI CAYNNNNRTG 1 cut(s) 651
SacI GAGCTC 1 cut(s) 643
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 3 cut(s) 230, 410, 753
SatI GCNGC 5 cut(s) 455, 458, 534, 665, 801
Sau96I GGNCC 1 cut(s) 615
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 1 cut(s) 855
SfcI CTRYAG 1 cut(s) 455
SfuI TTCGAA 1 cut(s) 816
SmiMI CAYNNNNRTG 1 cut(s) 651
Sse9I AATT 6 cut(s) 92, 284, 362, 383, 588, 874
SsiI CCGC 5 cut(s) 493, 506, 662, 748, 800
SspMI CTAG 3 cut(s) 33, 128, 461
SstI GAGCTC 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 116, 823
TaqI TCGA 1 cut(s) 816
TasI AATT 6 cut(s) 92, 284, 362, 383, 588, 874
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 4 cut(s) 392, 422, 695, 902
Tru1I TTAA 3 cut(s) 230, 410, 753
Tru9I TTAA 3 cut(s) 230, 410, 753
TscAI CASTG 1 cut(s) 508
TseI GCWGC 4 cut(s) 454, 457, 533, 664
TspDTI ATGAA 6 cut(s) 384, 414, 533, 581, 869, 897
TspRI CASTG 1 cut(s) 508
Van91I CCANNNNNTGG 1 cut(s) 481
XapI RAATTY 2 cut(s) 362, 588
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 3 cut(s) 33, 128, 461
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.