MD07G1117600.v1.1

coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004414
Physical Location & Seq
Reverse (-)
14433029 .. 14434285
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1117600.v1.1.491

Sequence Viewer

Length: 816 bp
ATGTCACAGAGCTTAGAAGAAGACCCTACACCACAGAGCTTGAAAGAACATAAATTACAGAGCTTGGAAAAAGGAGACCCTAAATCACAGAGTTTGAAAGAAGACTCTAAATCACAGAACGTGGAAGAAGGAGACTCTAAATCCCAGAGCTTGAAAGAAGACCCTAAATCACTCCGCTTGGAAGAAAACCCTAAATCACTCAGCTTGGAAGAAGACCCTAAATCACCGAGCTTGGAAGGACGAGATAAAATTGCATTCTCTGAATTGAATGAAAATTCATTGCAATTCCTGGATTCAATAGACGGGTATTTAACCCTATTGGATTCACTGTCCTGGACACTCCGGCAGGGGTGGTTGCAATTAGCAAGTGCTCGACATTCTATGGGCGAGTCACGCGTGAGCAGTGCTTTGTTGGACCTGAAATCCCACAATGCAAGTACATTTCTGCAAGTAACCCAGCAATCTGATACTGAAACAGAGCAAATTCATCATTTTACATTGCGCAAATGGGCATCCTCCGACAATGACAATGGAGCTCCACTTATGAATATGAGTCCACAGATACGACAACGAAAGGATAAGGATGGATCTCCACCCCCACTTAAAGCTGAAACTGATCACCAACATCAACTTCAGAAGGAGCGGACTAAGTCACTGTTGATGTTTGGTGGTTTGGTTTCGCCGAAGCTACGGGCAGCACAACTCTCATTTGAATCTGCACTTGACGCACTTGTAGAGATTGCCAACATGCGTTCTTTGATGCTTTCCATGTTTGATAAGGTCAGGGAAGAATTGGAGGCTGCCAAAGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

30.43

Weight (kDa)

5.39

Isoelectric Point (pI)

63.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma22_CCDC115 PF21730 91 - 239 1.1e-12 Vma22/CCDC115
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 503
AccBSI CCGCTC 1 cut(s) 643
AccII CGCG 1 cut(s) 396
AciI CCGC 2 cut(s) 175, 643
AclWI GGATC 1 cut(s) 595
AcsI RAATTY 2 cut(s) 274, 483
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 1 cut(s) 439
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 6 cut(s) 43, 97, 154, 268, 297, 713
AjnI CCWGG 2 cut(s) 288, 332
AjuI GAANNNNNNNTTGG 2 cut(s) 615, 647
AluBI AGCT 9 cut(s) 12, 39, 63, 150, 204, 231, 536, 608, 688
AluI AGCT 9 cut(s) 12, 39, 63, 150, 204, 231, 536, 608, 688
Alw21I GWGCWC 2 cut(s) 373, 538
Alw26I GTCTC 2 cut(s) 69, 126
AlwI GGATC 1 cut(s) 595
ApeKI GCWGC 2 cut(s) 695, 800
ApoI RAATTY 2 cut(s) 274, 483
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 216, 611
AvaII GGWCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 4 cut(s) 27, 108, 165, 219
Bbv12I GWGCWC 2 cut(s) 373, 538
BbvI GCAGC 2 cut(s) 707, 787
BccI CCATC 1 cut(s) 578
BciT130I CCWGG 2 cut(s) 290, 334
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 2 cut(s) 69, 126
BisI GCNGC 2 cut(s) 696, 801
BlsI GCNGC 2 cut(s) 697, 802
Bme1390I CCNGG 2 cut(s) 290, 334
Bme18I GGWCC 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 290, 334
BmsI GCATC 2 cut(s) 521, 750
BpiI GAAGAC 4 cut(s) 27, 108, 165, 219
BsaI GGTCTC 1 cut(s) 69
Bse3DI GCAATG 2 cut(s) 278, 497
BseBI CCWGG 2 cut(s) 290, 334
BseGI GGATG 2 cut(s) 512, 589
BseMI GCAATG 2 cut(s) 278, 497
BseMII CTCAG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 707, 787
BseYI CCCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 373, 538
BsiSI CCGG 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 69, 126
BsmI GAATGC 1 cut(s) 254
Bso31I GGTCTC 1 cut(s) 69
Bsp1286I GDGCHC 2 cut(s) 373, 538
Bsp143I GATC 2 cut(s) 587, 616
BspACI CCGC 2 cut(s) 175, 643
BspCNI CTCAG 1 cut(s) 213
BspFNI CGCG 1 cut(s) 396
BspPI GGATC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 69
BsrBI CCGCTC 1 cut(s) 643
BsrDI GCAATG 2 cut(s) 278, 497
BssMI GATC 2 cut(s) 587, 616
Bst2UI CCWGG 2 cut(s) 290, 334
Bst4CI ACNGT 2 cut(s) 330, 657
BstDEI CTNAG 3 cut(s) 13, 200, 648
BstF5I GGATG 2 cut(s) 512, 589
BstFNI CGCG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 2 cut(s) 590, 619
BstMAI GTCTC 2 cut(s) 69, 126
BstMBI GATC 2 cut(s) 587, 616
BstMWI GCNNNNNNNGC 3 cut(s) 393, 725, 806
BstNI CCWGG 2 cut(s) 290, 334
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 288, 332
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 2 cut(s) 707, 787
BstV2I GAAGAC 4 cut(s) 27, 108, 165, 219
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
BtsCI GGATG 2 cut(s) 512, 589
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 3 cut(s) 326, 409, 653
CfoI GCGC 1 cut(s) 504
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 734
Csp6I GTAC 1 cut(s) 438
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviAII CATG 2 cut(s) 748, 769
CviQI GTAC 1 cut(s) 438
DdeI CTNAG 3 cut(s) 13, 200, 648
DpnI GATC 2 cut(s) 589, 618
DpnII GATC 2 cut(s) 587, 616
Ecl136II GAGCTC 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 288, 332
EcoT38I GRGCYC 1 cut(s) 538
FaeI CATG 2 cut(s) 751, 772
FaiI YATR 6 cut(s) 51, 383, 545, 551, 749, 770
FatI CATG 2 cut(s) 747, 768
FbaI TGATCA 1 cut(s) 616
Fnu4HI GCNGC 2 cut(s) 696, 801
FokI GGATG 2 cut(s) 499, 596
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 2 cut(s) 696, 801
FspI TGCGCA 1 cut(s) 503
GlaI GCGC 1 cut(s) 503
GluI GCNGC 2 cut(s) 696, 801
GsaI CCCAGC 1 cut(s) 460
HapII CCGG 1 cut(s) 343
HgaI GACGC 1 cut(s) 734
HhaI GCGC 1 cut(s) 504
Hin1II CATG 2 cut(s) 751, 772
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HinfI GANTC 7 cut(s) 104, 134, 293, 323, 389, 553, 713
HpaII CCGG 1 cut(s) 343
HphI GGTGA 2 cut(s) 216, 611
Hpy166II GTNNAC 1 cut(s) 557
Hpy188I TCNGA 4 cut(s) 262, 466, 520, 636
Hpy8I GTNNAC 1 cut(s) 557
HpyAV CCTTC 3 cut(s) 122, 230, 631
HpyCH4III ACNGT 2 cut(s) 330, 657
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 6 cut(s) 254, 283, 358, 434, 448, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 393, 725, 806
HpyF3I CTNAG 3 cut(s) 13, 200, 648
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 2 cut(s) 751, 772
HspAI GCGC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 2 cut(s) 587, 616
LmnI GCTCC 3 cut(s) 533, 541, 640
Lsp1109I GCAGC 2 cut(s) 707, 787
LweI GCATC 2 cut(s) 521, 750
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 4 cut(s) 3, 390, 451, 651
MalI GATC 2 cut(s) 589, 618
MbiI CCGCTC 1 cut(s) 643
MboI GATC 2 cut(s) 587, 616
MboII GAAGA 9 cut(s) 29, 32, 113, 137, 170, 194, 221, 224, 800
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 2 cut(s) 373, 538
MluCI AATT 8 cut(s) 53, 249, 263, 274, 284, 359, 483, 791
MluI ACGCGT 1 cut(s) 394
MlyI GAGTC 4 cut(s) 98, 128, 398, 562
MmeI TCCRAC 2 cut(s) 393, 543
MnlI CCTC 2 cut(s) 526, 790
MseI TTAA 2 cut(s) 311, 603
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 2 cut(s) 290, 334
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 2 cut(s) 290, 334
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 3 cut(s) 393, 725, 806
NdeII GATC 2 cut(s) 587, 616
NlaIII CATG 2 cut(s) 751, 772
NmuCI GTSAC 3 cut(s) 3, 390, 651
NsbI TGCGCA 1 cut(s) 503
NspI RCATGY 1 cut(s) 751
PctI GAATGC 1 cut(s) 254
PfeI GAWTC 3 cut(s) 293, 323, 713
PflFI GACNNNGTC 1 cut(s) 649
PfoI TCCNGGA 2 cut(s) 288, 332
PkrI GCNGC 2 cut(s) 697, 802
PleI GAGTC 4 cut(s) 98, 128, 397, 561
PpsI GAGTC 4 cut(s) 98, 128, 397, 561
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 2 cut(s) 288, 332
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 2 cut(s) 288, 332
PspPI GGNCC 1 cut(s) 415
PsrI GAACNNNNNNTAC 2 cut(s) 39, 71
PsuI RGATCY 1 cut(s) 587
PsyI GACNNNGTC 1 cut(s) 649
RsaI GTAC 1 cut(s) 439
RsaNI GTAC 1 cut(s) 438
SacI GAGCTC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 311, 603
SatI GCNGC 2 cut(s) 696, 801
Sau3AI GATC 2 cut(s) 587, 616
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 4 cut(s) 98, 128, 398, 562
ScrFI CCNGG 2 cut(s) 290, 334
SduI GDGCHC 2 cut(s) 373, 538
SfaNI GCATC 2 cut(s) 521, 750
SinI GGWCC 1 cut(s) 415
Sse9I AATT 8 cut(s) 53, 249, 263, 274, 284, 359, 483, 791
SsiI CCGC 2 cut(s) 175, 643
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 2 cut(s) 288, 332
TaaI ACNGT 2 cut(s) 330, 657
TaiI ACGT 1 cut(s) 123
TaqI TCGA 1 cut(s) 373
TasI AATT 8 cut(s) 53, 249, 263, 274, 284, 359, 483, 791
TatI WGTACW 1 cut(s) 437
TfiI GAWTC 3 cut(s) 293, 323, 713
Tru1I TTAA 2 cut(s) 311, 603
Tru9I TTAA 2 cut(s) 311, 603
TscAI CASTG 3 cut(s) 333, 409, 660
TseFI GTSAC 3 cut(s) 3, 390, 651
TseI GCWGC 2 cut(s) 695, 800
Tsp45I GTSAC 3 cut(s) 3, 390, 651
TspDTI ATGAA 4 cut(s) 267, 285, 476, 560
TspRI CASTG 3 cut(s) 333, 409, 660
Tth111I GACNNNGTC 1 cut(s) 649
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 2 cut(s) 274, 483
XceI RCATGY 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.