Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004375

Physical Location & Seq

Genomic Coordinates Forward (+)

18668010 .. 18670576

Gene Structure 2567 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G50400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGGAGGGCTTTTCACCACCGATTAACACTGCGCAAGTCGATGCAAAGACCAAACTTGACGAGAAAGTGGATTACTCAAACCTCCCTTGCCCTGTCCTCTATGAGGAACTCAACCGTGAAGCTACCATGGCTTTAAAGCCAGAGCTATTTGAGGGTTTCCGCCTCGACTATAACAAGTCGCTTAACCAGAAGTTTTTTCTCAGCCATAGCATATTGATGGGGCCAACTGAGGTTCCTAATCCAACTCCATCATCCGAAATAATCAAAATTCCAACTGCCAATTATGACTTTGGTGCTGGTTTCATCGATCCAAAGCTATATCTTATCGGAAGGATCACGACGGACGGTAGACTAAATGCGAGAGCCAAGTTCGATTTAACTGACAATTTTTCCGTCAAGGCTAATGCTCTGCTGACAGATGAAGAAGATAAGTCGCAAGGCCACCTCGTCATTGATTACAAGGGGTCTGACTACAGAACTCAGCTTCAGCTTGGAAACAATTCCGTTTATGCAGCTAACTATATTCAGCATGTGACACCACACCTGTCTTTGGGTGGTGAGGCTTTCTGGCTTGGTCAGCAACTGATGTCGGGTGTTGGTTATGCAGCTCGATATGAGACTGACAAAACGGTTGCCTCTGGGCAAATTGCTTCCACTGGTGTGGCTGTCATGAACTATGTTCATAAGGTTTCAGAGAAGTTGTCATTTGCAACGGATTTCATCTACAATTATTTGTCACGAGATGTTACAGCTAGTGTCGGGTATGACCTCATTACTAGACAGTCTCGATTAAGAGGAAAGGTTGATTCTAACGGTGTTGTCGCTGCTTACCTTGAAGAACAATTGCCTATTGGACTCAGATTTCTTCTATCTGCAGAGGTGGATCATGTGAAGAAGGATTACAAGTTTGGTTTCGGTGTTAACGCCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005742 GO:0005743 GO:0005773 GO:0005774 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009987 GO:0015031 GO:0015399 GO:0015405 GO:0015450 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019866 GO:0019867 GO:0022804 GO:0022857 GO:0022884 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098588 GO:0098796 GO:0098798 GO:0098799 GO:0098805 GO:1904680 GO:1990542

KEGG Pathways

Metabolic & Signaling

ko05014 map05014

Pfam Domains

Protein Families

Porin_3

Protein Analysis

310

Amino Acids

34.47

Weight (kDa)

5.58

Isoelectric Point (pI)

26.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Porin_3	PF01459	30 - 304	2.9e-61	Eukaryotic porin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0004375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50400 AT3G20000 AT3G20000
fragaria_vesca	FvH4_4g11890
malus_domestica	MD04G1030900.v1.1 MD13G1002300.v1.1 MD13G1277300.v1.1
prunus_persica	Prupe.1G180200_v2.0.a1 Prupe.1G350100_v2.0.a1
pyrus_communis	pycom04g02610 pycom13g29500
rosa_chinensis	RchiOBHm_Chr4g0409321 RchiOBHm_Chr4g0447041
rosa_laevigata	RLG00000005610 RLG00000008570
rosa_multiflora	Rmu_sc0014071.1_g000007 Rmu_sc0019356.1_g000004
rosa_roxburghii	Rroxscaffold_5G00353830 Rroxscaffold_5G00387360
rosa_rugosa	Rorug04G0092300 Rorug04G0377400 Rorug04G0377500 Rorug04G0377600
rosa_samantha	Rh4AG155400 Rh4AG438700 Rh4BG153700 Rh4BG447800 Rh4CG164300 Rh4CG464900 Rh4DG148300
rosa_wichuraiana	Rw4G012690 Rw4G037530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	33
AccI	GTMKAC	1 cut(s)	349
AciI	CCGC	1 cut(s)	160
AclWI	GGATC	3 cut(s)	302, 341, 891
AcsI	RAATTY	1 cut(s)	267
AcuI	CTGAAG	1 cut(s)	470
AfiI	CCNNNNNNNGG	2 cut(s)	103, 550
AgsI	TTSAA	1 cut(s)	836
AleI	CACNNNNGTG	1 cut(s)	659
AluBI	AGCT	8 cut(s)	122, 145, 316, 484, 490, 515, 608, 752
AluI	AGCT	8 cut(s)	122, 145, 316, 484, 490, 515, 608, 752
Alw26I	GTCTC	2 cut(s)	611, 789
AlwI	GGATC	3 cut(s)	302, 341, 891
AlwNI	CAGNNNCTG	1 cut(s)	583
AoxI	GGCC	2 cut(s)	221, 439
ApeKI	GCWGC	3 cut(s)	512, 605, 824
ApoI	RAATTY	1 cut(s)	267
Asp700I	GAANNNNTTC	1 cut(s)	499
AspLEI	GCGC	1 cut(s)	34
AspS9I	GGNCC	1 cut(s)	221
AsuHPI	GGTGA	2 cut(s)	6, 569
BarI	GAAGNNNNNNTAC	2 cut(s)	884, 916
BauI	CACGAG	1 cut(s)	738
BbvI	GCAGC	3 cut(s)	524, 617, 811
BccI	CCATC	2 cut(s)	211, 256
BcoDI	GTCTC	2 cut(s)	611, 789
BfaI	CTAG	2 cut(s)	753, 777
BfmI	CTRYAG	2 cut(s)	472, 873
BisI	GCNGC	3 cut(s)	513, 606, 825
BlsI	GCNGC	3 cut(s)	514, 607, 826
BmgT120I	GGNCC	1 cut(s)	221
BmiI	GGNNCC	2 cut(s)	222, 234
BmsI	GCATC	1 cut(s)	31
Bsa29I	ATCGAT	1 cut(s)	306
BsaJI	CCNNGG	1 cut(s)	126
Bsc4I	CCNNNNNNNGG	2 cut(s)	103, 550
Bse1I	ACTGG	1 cut(s)	661
BseCI	ATCGAT	1 cut(s)	306
BseDI	CCNNGG	1 cut(s)	126
BseGI	GGATG	1 cut(s)	251
BseLI	CCNNNNNNNGG	2 cut(s)	103, 550
BseMII	CTCAG	4 cut(s)	214, 219, 494, 871
BseNI	ACTGG	1 cut(s)	661
BseXI	GCAGC	3 cut(s)	524, 617, 811
BshFI	GGCC	2 cut(s)	223, 441
BshVI	ATCGAT	1 cut(s)	306
BslI	CCNNNNNNNGG	2 cut(s)	103, 550
BsmAI	GTCTC	2 cut(s)	611, 789
BsnI	GGCC	2 cut(s)	223, 441
Bsp143I	GATC	3 cut(s)	307, 333, 883
Bsp19I	CCATGG	1 cut(s)	126
BspACI	CCGC	1 cut(s)	160
BspANI	GGCC	2 cut(s)	223, 441
BspCNI	CTCAG	4 cut(s)	213, 220, 493, 870
BspDI	ATCGAT	1 cut(s)	306
BspHI	TCATGA	1 cut(s)	669
BspLI	GGNNCC	2 cut(s)	222, 234
BspMAI	CTGCAG	1 cut(s)	877
BspPI	GGATC	3 cut(s)	302, 341, 891
BsrI	ACTGG	1 cut(s)	661
BssECI	CCNNGG	1 cut(s)	126
BssMI	GATC	3 cut(s)	307, 333, 883
BssSI	CACGAG	1 cut(s)	738
BssT1I	CCWWGG	1 cut(s)	126
Bst2BI	CACGAG	1 cut(s)	738
Bst4CI	ACNGT	5 cut(s)	116, 347, 631, 783, 815
BstDEI	CTNAG	4 cut(s)	200, 228, 480, 857
BstDSI	CCRYGG	1 cut(s)	126
BstENI	CCTNNNNNAGG	1 cut(s)	101
BstF5I	GGATG	1 cut(s)	251
BstHHI	GCGC	1 cut(s)	34
BstKTI	GATC	3 cut(s)	310, 336, 886
BstMAI	GTCTC	2 cut(s)	611, 789
BstMBI	GATC	3 cut(s)	307, 333, 883
BstMWI	GCNNNNNNNGC	2 cut(s)	128, 577
BstNSI	RCATGY	1 cut(s)	533
BstSFI	CTRYAG	2 cut(s)	472, 873
BstV1I	GCAGC	3 cut(s)	524, 617, 811
BstXI	CCANNNNNNTGG	1 cut(s)	661
Bsu15I	ATCGAT	1 cut(s)	306
BsuRI	GGCC	2 cut(s)	223, 441
BsuTUI	ATCGAT	1 cut(s)	306
BtgI	CCRYGG	1 cut(s)	126
BtsCI	GGATG	1 cut(s)	251
BtsI	GCAGTG	1 cut(s)	27
BtsIMutI	CAGTG	2 cut(s)	27, 654
CaiI	CAGNNNCTG	1 cut(s)	583
CciI	TCATGA	1 cut(s)	669
CfoI	GCGC	1 cut(s)	34
Cfr13I	GGNCC	1 cut(s)	221
ClaI	ATCGAT	1 cut(s)	306
CviAII	CATG	4 cut(s)	127, 530, 670, 887
DdeI	CTNAG	4 cut(s)	200, 228, 480, 857
DpnI	GATC	3 cut(s)	309, 335, 885
DpnII	GATC	3 cut(s)	307, 333, 883
DraI	TTTAAA	1 cut(s)	135
EciI	GGCGGA	1 cut(s)	149
Eco130I	CCWWGG	1 cut(s)	126
Eco57I	CTGAAG	1 cut(s)	470
EcoNI	CCTNNNNNAGG	1 cut(s)	101
EcoT14I	CCWWGG	1 cut(s)	126
ErhI	CCWWGG	1 cut(s)	126
FaeI	CATG	4 cut(s)	130, 533, 673, 890
FatI	CATG	4 cut(s)	126, 529, 669, 886
FblI	GTMKAC	1 cut(s)	349
Fnu4HI	GCNGC	3 cut(s)	513, 606, 825
FokI	GGATG	1 cut(s)	238
Fsp4HI	GCNGC	3 cut(s)	513, 606, 825
FspBI	CTAG	2 cut(s)	753, 777
FspI	TGCGCA	1 cut(s)	33
GlaI	GCGC	1 cut(s)	33
GluI	GCNGC	3 cut(s)	513, 606, 825
HaeIII	GGCC	2 cut(s)	223, 441
HhaI	GCGC	1 cut(s)	34
Hin1II	CATG	4 cut(s)	130, 533, 673, 890
Hin6I	GCGC	1 cut(s)	32
HinP1I	GCGC	1 cut(s)	32
HincII	GTYRAC	1 cut(s)	922
HindII	GTYRAC	1 cut(s)	922
HinfI	GANTC	2 cut(s)	806, 855
HpaI	GTTAAC	1 cut(s)	922
HphI	GGTGA	2 cut(s)	6, 569
Hpy166II	GTNNAC	2 cut(s)	350, 922
Hpy188I	TCNGA	5 cut(s)	256, 329, 469, 694, 860
Hpy188III	TCNNGA	4 cut(s)	337, 670, 738, 786
Hpy8I	GTNNAC	2 cut(s)	350, 922
Hpy99I	CGWCG	1 cut(s)	343
HpyAV	CCTTC	2 cut(s)	324, 889
HpyCH4III	ACNGT	5 cut(s)	116, 347, 631, 783, 815
HpyCH4V	TGCA	5 cut(s)	44, 512, 605, 710, 875
HpyF10VI	GCNNNNNNNGC	2 cut(s)	128, 577
HpyF3I	CTNAG	4 cut(s)	200, 228, 480, 857
Hsp92II	CATG	4 cut(s)	130, 533, 673, 890
HspAI	GCGC	1 cut(s)	32
KspAI	GTTAAC	1 cut(s)	922
Kzo9I	GATC	3 cut(s)	307, 333, 883
LpnPI	CCDG	8 cut(s)	105, 153, 200, 282, 553, 557, 624, 642
Lsp1109I	GCAGC	3 cut(s)	524, 617, 811
LweI	GCATC	1 cut(s)	31
MaeI	CTAG	2 cut(s)	753, 777
MaeIII	GTNAC	3 cut(s)	532, 735, 745
MalI	GATC	3 cut(s)	309, 335, 885
MboI	GATC	3 cut(s)	307, 333, 883
MboII	GAAGA	5 cut(s)	434, 437, 848, 857, 904
MfeI	CAATTG	1 cut(s)	842
MluCI	AATT	7 cut(s)	267, 280, 385, 499, 645, 727, 842
MlyI	GAGTC	1 cut(s)	849
MmeI	TCCRAC	2 cut(s)	266, 296
MroXI	GAANNNNTTC	1 cut(s)	499
MseI	TTAA	7 cut(s)	24, 134, 183, 377, 791, 921, 931
MslI	CAYNNNNRTG	2 cut(s)	215, 659
MunI	CAATTG	1 cut(s)	842
MwoI	GCNNNNNNNGC	2 cut(s)	128, 577
NcoI	CCATGG	1 cut(s)	126
NdeII	GATC	3 cut(s)	307, 333, 883
NlaIII	CATG	4 cut(s)	130, 533, 673, 890
NlaIV	GGNNCC	2 cut(s)	222, 234
NmuCI	GTSAC	2 cut(s)	532, 735
NsbI	TGCGCA	1 cut(s)	33
NspI	RCATGY	1 cut(s)	533
OliI	CACNNNNGTG	1 cut(s)	659
PagI	TCATGA	1 cut(s)	669
PdmI	GAANNNNTTC	1 cut(s)	499
PfeI	GAWTC	1 cut(s)	806
PkrI	GCNGC	3 cut(s)	514, 607, 826
PleI	GAGTC	1 cut(s)	849
PpsI	GAGTC	1 cut(s)	849
PspN4I	GGNNCC	2 cut(s)	222, 234
PspPI	GGNCC	1 cut(s)	221
PstI	CTGCAG	1 cut(s)	877
PstNI	CAGNNNCTG	1 cut(s)	583
RseI	CAYNNNNRTG	2 cut(s)	215, 659
SaqAI	TTAA	7 cut(s)	24, 134, 183, 377, 791, 921, 931
SatI	GCNGC	3 cut(s)	513, 606, 825
Sau3AI	GATC	3 cut(s)	307, 333, 883
Sau96I	GGNCC	1 cut(s)	221
SchI	GAGTC	1 cut(s)	849
SfaNI	GCATC	1 cut(s)	31
SfcI	CTRYAG	2 cut(s)	472, 873
SmiMI	CAYNNNNRTG	2 cut(s)	215, 659
Sse9I	AATT	7 cut(s)	267, 280, 385, 499, 645, 727, 842
SsiI	CCGC	1 cut(s)	160
SspMI	CTAG	2 cut(s)	753, 777
StyI	CCWWGG	1 cut(s)	126
TaaI	ACNGT	5 cut(s)	116, 347, 631, 783, 815
TaqI	TCGA	6 cut(s)	39, 165, 306, 372, 610, 787
TasI	AATT	7 cut(s)	267, 280, 385, 499, 645, 727, 842
TfiI	GAWTC	1 cut(s)	806
Tru1I	TTAA	7 cut(s)	24, 134, 183, 377, 791, 921, 931
Tru9I	TTAA	7 cut(s)	24, 134, 183, 377, 791, 921, 931
TscAI	CASTG	2 cut(s)	34, 661
TseFI	GTSAC	2 cut(s)	532, 735
TseI	GCWGC	3 cut(s)	512, 605, 824
Tsp45I	GTSAC	2 cut(s)	532, 735
TspDTI	ATGAA	5 cut(s)	292, 435, 671, 686, 709
TspGWI	ACGGA	4 cut(s)	356, 382, 493, 728
TspRI	CASTG	2 cut(s)	34, 661
XagI	CCTNNNNNAGG	1 cut(s)	101
XapI	RAATTY	1 cut(s)	267
XceI	RCATGY	1 cut(s)	533
XmiI	GTMKAC	1 cut(s)	349
XmnI	GAANNNNTTC	1 cut(s)	499
XspI	CTAG	2 cut(s)	753, 777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.