Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004375

Physical Location & Seq

Genomic Coordinates Forward (+)

3455001 .. 3462159

Gene Structure 7159 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1030900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGGCGGGCCTCGTTCCTCCTCCTCCTCCAACCGCCGCGGCTGATACGATTCCGAAGCCGAAAGAGGATGAGAAAGTGGATTACTTGAATCTTCCTTGCCCTATTCCCTACGAGGAGATCCACCGTGAAGCTCTCATGTCTTTAAAGCCGGAACTGTTTGAGGGGATGCGCTTTGATTTCACTAAAGGACTCAATCAGAGGTTCTCACTCAGTCACAGCGTGTTCATGGGACCTACCGAGGTTCCTTCACAATCTCCCGAAACTATTAAAATTCCCACTGCTCACTATGAGTTTGGTGCCAACTTTATAGATCCAAAGTTGATGCTTTTTGGGAGAATCATGACTGATGGGAGGCTTAATGCAAGAGTAAAGTGCGATTTATCTGAGAATCTTACTTTGAAGGCCAATTCTCAGCTTACCAACGAGCCTCACATGTCACATGGCATGGTCAATTTTGATTACAAGGGAAAAGATTACAGGTCACAGTTTCAACTAGGAAATGGTGCACTGTTTGGAGCAAACTACATTCAGAGTGTGACCCCCCATCTCGCTTTGGGCGGTGAAGTGTTCTGGGCTGGTCAGCATCGGAAGTCTGGTATTGGCTATGCTGCTCGGTATAACACAGAAAAGATGGTTGCCACAGGGCAAGTCGCTAGCACTGGAATGGTTGCTCTTAGCTATGTACAAAAGGTTTCTGATAAGGTTTCTCTTGCATCAGATTTCATGTACAACTACATGTCAAGAGATGTCACAGCTAGTTTTGGTTATGATTACATTCTTCGACAGTGCCGTCTTAGAGGAAAGATTGATTCCAATGGCACCGTAGCTGCATTTTTGGAAGAGCGACTAAATATGGGTCTTAATTTTGTTCTTTCTGCAGAGATTGATCACAGAAAGAAAGACTACAAATTCGGGTTTGGGTTGACTGTCGGGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005742 GO:0005743 GO:0005773 GO:0005774 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009987 GO:0015031 GO:0015399 GO:0015405 GO:0015450 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019866 GO:0019867 GO:0022804 GO:0022857 GO:0022884 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098588 GO:0098796 GO:0098798 GO:0098799 GO:0098805 GO:1904680 GO:1990542

KEGG Pathways

Metabolic & Signaling

ko05014 map05014

Pfam Domains

Protein Families

Porin_3

Protein Analysis

313

Amino Acids

34.81

Weight (kDa)

7.09

Isoelectric Point (pI)

29.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Porin_3	PF01459	33 - 305	2.1e-67	Eukaryotic porin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50400 AT3G20000 AT3G20000
fragaria_vesca	FvH4_4g11890
malus_domestica	MD04G1030900.v1.1 MD13G1002300.v1.1 MD13G1277300.v1.1
prunus_persica	Prupe.1G180200_v2.0.a1 Prupe.1G350100_v2.0.a1
pyrus_communis	pycom04g02610 pycom13g29500
rosa_chinensis	RchiOBHm_Chr4g0409321 RchiOBHm_Chr4g0447041
rosa_laevigata	RLG00000005610 RLG00000008570
rosa_multiflora	Rmu_sc0014071.1_g000007 Rmu_sc0019356.1_g000004
rosa_roxburghii	Rroxscaffold_5G00353830 Rroxscaffold_5G00387360
rosa_rugosa	Rorug04G0092300 Rorug04G0377400 Rorug04G0377500 Rorug04G0377600
rosa_samantha	Rh4AG155400 Rh4AG438700 Rh4BG153700 Rh4BG447800 Rh4CG164300 Rh4CG464900 Rh4DG148300
rosa_wichuraiana	Rw4G012690 Rw4G037530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	789
AccB1I	GGYRCC	2 cut(s)	296, 818
AccII	CGCG	1 cut(s)	38
AciI	CCGC	5 cut(s)	5, 33, 36, 38, 558
AclWI	GGATC	2 cut(s)	112, 305
AcsI	RAATTY	2 cut(s)	270, 908
AdeI	CACNNNGTG	1 cut(s)	220
AfaI	GTAC	2 cut(s)	684, 728
AflIII	ACRYGT	2 cut(s)	432, 735
AgsI	TTSAA	3 cut(s)	88, 400, 491
AluBI	AGCT	5 cut(s)	131, 415, 678, 755, 827
AluI	AGCT	5 cut(s)	131, 415, 678, 755, 827
Alw21I	GWGCWC	1 cut(s)	508
Alw44I	GTGCAC	1 cut(s)	504
AlwI	GGATC	2 cut(s)	112, 305
AoxI	GGCC	2 cut(s)	7, 402
ApaLI	GTGCAC	1 cut(s)	504
ApeKI	GCWGC	2 cut(s)	608, 827
ApoI	RAATTY	2 cut(s)	270, 908
ArsI	GACNNNNNNTTYG	2 cut(s)	893, 925
AspLEI	GCGC	1 cut(s)	171
AspS9I	GGNCC	2 cut(s)	7, 230
AsuHPI	GGTGA	1 cut(s)	572
AsuNHI	GCTAGC	1 cut(s)	653
AvaII	GGWCC	1 cut(s)	230
BaeGI	GKGCMC	1 cut(s)	508
BanI	GGYRCC	2 cut(s)	296, 818
Bbv12I	GWGCWC	1 cut(s)	508
BbvI	GCAGC	2 cut(s)	595, 814
BccI	CCATC	3 cut(s)	341, 552, 625
BceAI	ACGGC	1 cut(s)	774
BclI	TGATCA	1 cut(s)	886
BfaI	CTAG	3 cut(s)	494, 654, 756
BfmI	CTRYAG	1 cut(s)	876
BisI	GCNGC	4 cut(s)	36, 39, 609, 828
BlsI	GCNGC	4 cut(s)	37, 40, 610, 829
Bme18I	GGWCC	1 cut(s)	230
BmgT120I	GGNCC	2 cut(s)	7, 230
BmiI	GGNNCC	4 cut(s)	231, 243, 298, 820
BmsI	GCATC	4 cut(s)	156, 312, 592, 722
BmtI	GCTAGC	1 cut(s)	657
BsaJI	CCNNGG	2 cut(s)	36, 237
Bse1I	ACTGG	1 cut(s)	664
BseDI	CCNNGG	2 cut(s)	36, 237
BseGI	GGATG	2 cut(s)	73, 171
BseMII	CTCAG	3 cut(s)	223, 375, 425
BseNI	ACTGG	1 cut(s)	664
BseRI	GAGGAG	4 cut(s)	9, 12, 15, 128
BseSI	GKGCMC	1 cut(s)	508
BseXI	GCAGC	2 cut(s)	595, 814
Bsh1236I	CGCG	1 cut(s)	38
BshFI	GGCC	2 cut(s)	9, 404
BshNI	GGYRCC	2 cut(s)	296, 818
BsiHKAI	GWGCWC	1 cut(s)	508
BsiSI	CCGG	1 cut(s)	149
BslFI	GGGAC	1 cut(s)	243
BsmFI	GGGAC	1 cut(s)	243
BsnI	GGCC	2 cut(s)	9, 404
Bsp1286I	GDGCHC	1 cut(s)	508
Bsp1407I	TGTACA	2 cut(s)	682, 726
Bsp143I	GATC	3 cut(s)	117, 310, 886
BspACI	CCGC	5 cut(s)	5, 33, 36, 38, 558
BspANI	GGCC	2 cut(s)	9, 404
BspCNI	CTCAG	3 cut(s)	222, 376, 424
BspFNI	CGCG	1 cut(s)	38
BspHI	TCATGA	1 cut(s)	339
BspLI	GGNNCC	4 cut(s)	231, 243, 298, 820
BspMAI	CTGCAG	1 cut(s)	880
BspOI	GCTAGC	1 cut(s)	657
BspPI	GGATC	2 cut(s)	112, 305
BspQI	GCTCTTC	1 cut(s)	834
BspT107I	GGYRCC	2 cut(s)	296, 818
BsrGI	TGTACA	2 cut(s)	682, 726
BsrI	ACTGG	1 cut(s)	664
BssECI	CCNNGG	2 cut(s)	36, 237
BssMI	GATC	3 cut(s)	117, 310, 886
Bst4CI	ACNGT	7 cut(s)	125, 156, 486, 510, 786, 823, 928
Bst6I	CTCTTC	1 cut(s)	834
BstAUI	TGTACA	2 cut(s)	682, 726
BstC8I	GCNNGC	2 cut(s)	7, 655
BstDEI	CTNAG	5 cut(s)	209, 384, 411, 674, 794
BstDSI	CCRYGG	1 cut(s)	36
BstF5I	GGATG	2 cut(s)	73, 171
BstFNI	CGCG	1 cut(s)	38
BstHHI	GCGC	1 cut(s)	171
BstKTI	GATC	3 cut(s)	120, 313, 889
BstMBI	GATC	3 cut(s)	117, 310, 886
BstNSI	RCATGY	2 cut(s)	436, 739
BstSFI	CTRYAG	1 cut(s)	876
BstSLI	GKGCMC	1 cut(s)	508
BstUI	CGCG	1 cut(s)	38
BstV1I	GCAGC	2 cut(s)	595, 814
BstX2I	RGATCY	2 cut(s)	117, 310
BstYI	RGATCY	2 cut(s)	117, 310
BsuRI	GGCC	2 cut(s)	9, 404
BtgI	CCRYGG	1 cut(s)	36
BtsCI	GGATG	2 cut(s)	73, 171
BtsI	GCAGTG	1 cut(s)	276
BtsIMutI	CAGTG	4 cut(s)	276, 506, 657, 791
Cac8I	GCNNGC	2 cut(s)	7, 655
CciI	TCATGA	1 cut(s)	339
CfoI	GCGC	1 cut(s)	171
Cfr13I	GGNCC	2 cut(s)	7, 230
Cfr42I	CCGCGG	1 cut(s)	39
Csp6I	GTAC	2 cut(s)	683, 727
CviAII	CATG	8 cut(s)	136, 226, 340, 433, 440, 445, 724, 736
CviQI	GTAC	2 cut(s)	683, 727
DdeI	CTNAG	5 cut(s)	209, 384, 411, 674, 794
DpnI	GATC	3 cut(s)	119, 312, 888
DpnII	GATC	3 cut(s)	117, 310, 886
DraI	TTTAAA	1 cut(s)	144
DraIII	CACNNNGTG	1 cut(s)	220
DrdI	GACNNNNNNGTC	1 cut(s)	789
DseDI	GACNNNNNNGTC	1 cut(s)	789
Eam1104I	CTCTTC	1 cut(s)	834
EarI	CTCTTC	1 cut(s)	834
Eco47I	GGWCC	1 cut(s)	230
EcoO109I	RGGNCCY	1 cut(s)	230
FaeI	CATG	8 cut(s)	139, 229, 343, 436, 443, 448, 727, 739
FaqI	GGGAC	1 cut(s)	243
FatI	CATG	8 cut(s)	135, 225, 339, 432, 439, 444, 723, 735
FbaI	TGATCA	1 cut(s)	886
Fnu4HI	GCNGC	4 cut(s)	36, 39, 609, 828
FokI	GGATG	2 cut(s)	80, 178
Fsp4HI	GCNGC	4 cut(s)	36, 39, 609, 828
FspBI	CTAG	3 cut(s)	494, 654, 756
GlaI	GCGC	1 cut(s)	170
GluI	GCNGC	4 cut(s)	36, 39, 609, 828
HaeIII	GGCC	2 cut(s)	9, 404
HapII	CCGG	1 cut(s)	149
HhaI	GCGC	1 cut(s)	171
Hin1II	CATG	8 cut(s)	139, 229, 343, 436, 443, 448, 727, 739
Hin6I	GCGC	1 cut(s)	169
HinP1I	GCGC	1 cut(s)	169
HincII	GTYRAC	1 cut(s)	924
HindII	GTYRAC	1 cut(s)	924
HinfI	GANTC	6 cut(s)	49, 88, 189, 336, 388, 809
HpaII	CCGG	1 cut(s)	149
HphI	GGTGA	1 cut(s)	572
Hpy166II	GTNNAC	2 cut(s)	506, 924
Hpy188I	TCNGA	7 cut(s)	54, 198, 385, 531, 588, 697, 718
Hpy188III	TCNNGA	3 cut(s)	257, 340, 741
Hpy8I	GTNNAC	2 cut(s)	506, 924
HpyAV	CCTTC	2 cut(s)	255, 394
HpyCH4III	ACNGT	7 cut(s)	125, 156, 486, 510, 786, 823, 928
HpyCH4V	TGCA	5 cut(s)	362, 506, 713, 830, 878
HpyF3I	CTNAG	5 cut(s)	209, 384, 411, 674, 794
Hsp92II	CATG	8 cut(s)	139, 229, 343, 436, 443, 448, 727, 739
HspAI	GCGC	1 cut(s)	169
Ksp22I	TGATCA	1 cut(s)	886
KspI	CCGCGG	1 cut(s)	39
Kzo9I	GATC	3 cut(s)	117, 310, 886
LguI	GCTCTTC	1 cut(s)	834
LmnI	GCTCC	1 cut(s)	515
LpnPI	CCDG	7 cut(s)	162, 463, 556, 561, 579, 627, 645
Lsp1109I	GCAGC	2 cut(s)	595, 814
LweI	GCATC	4 cut(s)	156, 312, 592, 722
MaeI	CTAG	3 cut(s)	494, 654, 756
MaeIII	GTNAC	5 cut(s)	212, 435, 480, 535, 748
MalI	GATC	3 cut(s)	119, 312, 888
MboI	GATC	3 cut(s)	117, 310, 886
MboII	GAAGA	3 cut(s)	83, 770, 851
MflI	RGATCY	2 cut(s)	117, 310
MhlI	GDGCHC	1 cut(s)	508
MluCI	AATT	5 cut(s)	270, 406, 451, 862, 908
MlyI	GAGTC	1 cut(s)	183
MmeI	TCCRAC	1 cut(s)	53
MseI	TTAA	4 cut(s)	143, 267, 357, 861
MslI	CAYNNNNRTG	1 cut(s)	662
MspA1I	CMGCKG	1 cut(s)	38
MspI	CCGG	1 cut(s)	149
MvnI	CGCG	1 cut(s)	38
NdeII	GATC	3 cut(s)	117, 310, 886
NheI	GCTAGC	1 cut(s)	653
NlaIII	CATG	8 cut(s)	139, 229, 343, 436, 443, 448, 727, 739
NlaIV	GGNNCC	4 cut(s)	231, 243, 298, 820
NmuCI	GTSAC	5 cut(s)	212, 435, 480, 535, 748
NspI	RCATGY	2 cut(s)	436, 739
PagI	TCATGA	1 cut(s)	339
PciI	ACATGT	2 cut(s)	432, 735
PciSI	GCTCTTC	1 cut(s)	834
PcsI	WCGNNNNNNNCGW	1 cut(s)	787
PfeI	GAWTC	5 cut(s)	49, 88, 336, 388, 809
PkrI	GCNGC	4 cut(s)	37, 40, 610, 829
PleI	GAGTC	1 cut(s)	183
PpsI	GAGTC	1 cut(s)	183
PpuMI	RGGWCCY	1 cut(s)	230
PscI	ACATGT	2 cut(s)	432, 735
Psp5II	RGGWCCY	1 cut(s)	230
PspN4I	GGNNCC	4 cut(s)	231, 243, 298, 820
PspPI	GGNCC	2 cut(s)	7, 230
PspPPI	RGGWCCY	1 cut(s)	230
PstI	CTGCAG	1 cut(s)	880
PsuI	RGATCY	2 cut(s)	117, 310
RsaI	GTAC	2 cut(s)	684, 728
RsaNI	GTAC	2 cut(s)	683, 727
RseI	CAYNNNNRTG	1 cut(s)	662
SacII	CCGCGG	1 cut(s)	39
SapI	GCTCTTC	1 cut(s)	834
SaqAI	TTAA	4 cut(s)	143, 267, 357, 861
SatI	GCNGC	4 cut(s)	36, 39, 609, 828
Sau3AI	GATC	3 cut(s)	117, 310, 886
Sau96I	GGNCC	2 cut(s)	7, 230
SchI	GAGTC	1 cut(s)	183
SduI	GDGCHC	1 cut(s)	508
SfaNI	GCATC	4 cut(s)	156, 312, 592, 722
SfcI	CTRYAG	1 cut(s)	876
Sfr303I	CCGCGG	1 cut(s)	39
SgrBI	CCGCGG	1 cut(s)	39
SinI	GGWCC	1 cut(s)	230
SmiMI	CAYNNNNRTG	1 cut(s)	662
Sse9I	AATT	5 cut(s)	270, 406, 451, 862, 908
SsiI	CCGC	5 cut(s)	5, 33, 36, 38, 558
SspMI	CTAG	3 cut(s)	494, 654, 756
TaaI	ACNGT	7 cut(s)	125, 156, 486, 510, 786, 823, 928
TaqI	TCGA	1 cut(s)	781
TasI	AATT	5 cut(s)	270, 406, 451, 862, 908
TatI	WGTACW	2 cut(s)	682, 726
TauI	GCSGC	2 cut(s)	38, 41
TfiI	GAWTC	5 cut(s)	49, 88, 336, 388, 809
Tru1I	TTAA	4 cut(s)	143, 267, 357, 861
Tru9I	TTAA	4 cut(s)	143, 267, 357, 861
TscAI	CASTG	4 cut(s)	283, 513, 664, 791
TseFI	GTSAC	5 cut(s)	212, 435, 480, 535, 748
TseI	GCWGC	2 cut(s)	608, 827
Tsp45I	GTSAC	5 cut(s)	212, 435, 480, 535, 748
TspDTI	ATGAA	2 cut(s)	214, 712
TspRI	CASTG	4 cut(s)	283, 513, 664, 791
VneI	GTGCAC	1 cut(s)	504
VpaK11BI	GGWCC	1 cut(s)	230
XapI	RAATTY	2 cut(s)	270, 908
XceI	RCATGY	2 cut(s)	436, 739
XspI	CTAG	3 cut(s)	494, 654, 756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.