AT1G54150

E3 Ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002439
Physical Location & Seq
Forward (+)
20215395 .. 20217489
2095 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54150.1

Sequence Viewer

Length: 1152 bp
ATGTCCTCGCCGGAGCGTGCTCTCTTGAATCTATTGACGGACATCGCTCTCTCCTTCGACGGCGCAATCCTCGGACTGACTTTAGCTGTTAGCGCCGTCGGTTCTGCTCTCAAGTATGCTTCTACCAACGCCGCGTTGAAGAAGATCAAAGATGCACCCGAAGTCTCCATTTCCGACCTCCGATCACTGCTTCCGGCTTCTGAAGACAAATCAGAGACTAACGATAACAGGAAGAGCAATGATCAGCGAATCGTTGTGGTTCGCGGAGTCGTCAAACCGAAAATTTCCGGTGATGAGGGTTACAAGAACAACAACGTGTTAATCTCTCCGGAGACTGGAGATAAAGCTCTTATCATTCAGAGAACGCAAACTTATGTGTATAGTGGATGGAAGAGATTGTTTCAGTCCACTGGTCACCGGTTTATGTTGGAGAGATCATTGCGCAAACACGGTGCTGATTTTACGAGAACGGTCCCATTTGTCATTGTTGGAAAGGATCAGCAATCGAACTCTAGTTTTGTCGCTGTTAACATGGATGGCTCAAGGCAACCTCTTCCGCTTACCACTGTGTATAATCGCTTGCAGCCTATTAATTCTTCGTTTCTTCAAGCCTTTTTGTACCCCGATTATCCTGTTGGTCTGCTTGATATAGAGAAGATTTTACCACCTGGGAAAGATATAACAGCTGTTGGCATCTACAGTTTTAATAATGGAGTTCCTGAAATCAAATCGTGCCAAGATCTTCCTTATTTCTTATCAGAGATGACCAAGGACAAGATGATTGAGGACCTTATGGAACAGACCAACTTTATATTCTTGGGCAGCGTTATCTTGGGCATTGTGTCTGTTGGCATCCTTAGCTATGCTGCTGTCAGGACCTGGAATAAATGGAAACAATGGAACCATCAGAGAGAACTGCCACAGAGACCGAACGATTCTGTGGTCGATGATGAACCCGAAGATGCAGATGAAATCCCAGATGGAGAATTGTGTGTGATCTGCGTGTCAAGAAGGAGAGTACCTGCGTTTATTCCCTGTGGACATGTAGTATGTTGCAGGCGATGTGCTTCAACCGTGGAACGAGAGTTAAACCCTAAGTGTCCAGTTTGTCTTCAGAGCATTAGGGGATCTATGCGTGTATATTACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0001558 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005777 GO:0006464 GO:0006807 GO:0006919 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009893 GO:0009941 GO:0009966 GO:0009967 GO:0009987 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0016020 GO:0016021 GO:0016043 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019867 GO:0023051 GO:0023056 GO:0030162 GO:0030308 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031325 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032268 GO:0032270 GO:0032446 GO:0032592 GO:0036211 GO:0040008 GO:0042579 GO:0042802 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043122 GO:0043123 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0043412 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045862 GO:0045926 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051646 GO:0052547 GO:0052548 GO:0060255 GO:0065007 GO:0065009 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0098573 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000116 GO:2001056
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

383

Amino Acids

42.7

Weight (kDa)

8.0

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 141 - 285 2.4e-38 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 328 - 375 2.7e-12 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 1030
AccII CGCG 2 cut(s) 134, 264
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 3 cut(s) 132, 264, 557
AclWI GGATC 2 cut(s) 504, 1135
AcsI RAATTY 1 cut(s) 282
AcuI CTGAAG 2 cut(s) 222, 1097
AfaI GTAC 2 cut(s) 620, 1020
AfiI CCNNNNNNNGG 1 cut(s) 335
AflIII ACRYGT 2 cut(s) 315, 1042
AgeI ACCGGT 1 cut(s) 417
AgsI TTSAA 4 cut(s) 28, 139, 608, 1071
AjnI CCWGG 2 cut(s) 667, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AluBI AGCT 4 cut(s) 86, 347, 686, 861
AluI AGCT 4 cut(s) 86, 347, 686, 861
Alw21I GWGCWC 1 cut(s) 22
Alw26I GTCTC 4 cut(s) 169, 209, 326, 919
AlwI GGATC 2 cut(s) 504, 1135
AlwNI CAGNNNCTG 1 cut(s) 879
Aor13HI TCCGGA 1 cut(s) 328
ApeKI GCWGC 3 cut(s) 583, 822, 866
ApoI RAATTY 1 cut(s) 282
AseI ATTAAT 1 cut(s) 591
AsiGI ACCGGT 1 cut(s) 417
AspLEI GCGC 3 cut(s) 65, 95, 444
AspS9I GGNCC 3 cut(s) 472, 787, 876
AsuHPI GGTGA 2 cut(s) 302, 407
AvaII GGWCC 3 cut(s) 472, 787, 876
BbsI GAAGAC 2 cut(s) 210, 1103
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 3 cut(s) 595, 834, 853
BccI CCATC 4 cut(s) 381, 530, 912, 974
BceAI ACGGC 2 cut(s) 76, 80
BciT130I CCWGG 2 cut(s) 669, 880
BclI TGATCA 1 cut(s) 241
BcoDI GTCTC 4 cut(s) 169, 209, 326, 919
BfaI CTAG 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 697
BfoI RGCGCY 1 cut(s) 96
BfuAI ACCTGC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 739
BisI GCNGC 4 cut(s) 132, 584, 823, 867
BlsI GCNGC 4 cut(s) 133, 585, 824, 868
Bme1390I CCNGG 2 cut(s) 669, 880
Bme18I GGWCC 3 cut(s) 472, 787, 876
BmgT120I GGNCC 3 cut(s) 472, 787, 876
BmiI GGNNCC 2 cut(s) 474, 902
BmrFI CCNGG 2 cut(s) 669, 880
BmsI GCATC 4 cut(s) 142, 702, 861, 952
BpiI GAAGAC 2 cut(s) 210, 1103
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 857
BpuEI CTTGAG 2 cut(s) 95, 526
BsaI GGTCTC 1 cut(s) 919
BsaJI CCNNGG 4 cut(s) 70, 668, 768, 1074
BsaWI WCCGGW 3 cut(s) 287, 328, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse118I RCCGGY 1 cut(s) 417
Bse1I ACTGG 3 cut(s) 340, 415, 1103
Bse3DI GCAATG 2 cut(s) 244, 437
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 2 cut(s) 669, 880
BseDI CCNNGG 4 cut(s) 70, 668, 768, 1074
BseGI GGATG 3 cut(s) 392, 541, 852
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 2 cut(s) 244, 437
BseNI ACTGG 3 cut(s) 340, 415, 1103
BseXI GCAGC 3 cut(s) 595, 834, 853
Bsh1236I CGCG 2 cut(s) 134, 264
BshTI ACCGGT 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 22
BsiSI CCGG 5 cut(s) 11, 194, 288, 329, 418
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 4 cut(s) 169, 209, 326, 919
BsmFI GGGAC 1 cut(s) 458
Bso31I GGTCTC 1 cut(s) 919
Bsp1286I GDGCHC 1 cut(s) 22
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
BspACI CCGC 3 cut(s) 132, 264, 557
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 2 cut(s) 134, 264
BspLI GGNNCC 2 cut(s) 474, 902
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 2 cut(s) 504, 1135
BspQI GCTCTTC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 919
BsrDI GCAATG 2 cut(s) 244, 437
BsrFI RCCGGY 1 cut(s) 417
BsrI ACTGG 3 cut(s) 340, 415, 1103
BssAI RCCGGY 1 cut(s) 417
BssECI CCNNGG 4 cut(s) 70, 668, 768, 1074
BssMI GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 2 cut(s) 669, 880
Bst4CI ACNGT 5 cut(s) 452, 472, 568, 701, 1075
Bst6I CTCTTC 3 cut(s) 227, 386, 558
BstC8I GCNNGC 3 cut(s) 18, 581, 1058
BstDEI CTNAG 3 cut(s) 857, 1095, 1149
BstDSI CCRYGG 1 cut(s) 1074
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 3 cut(s) 392, 541, 852
BstFNI CGCG 2 cut(s) 134, 264
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 3 cut(s) 65, 95, 444
BstKTI GATC 8 cut(s) 147, 185, 244, 437, 499, 742, 999, 1130
BstMAI GTCTC 4 cut(s) 169, 209, 326, 919
BstMBI GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
BstMWI GCNNNNNNNGC 2 cut(s) 92, 858
BstNI CCWGG 2 cut(s) 669, 880
BstNSI RCATGY 1 cut(s) 1046
BstPI GGTNACC 1 cut(s) 413
BstSCI CCNGG 2 cut(s) 667, 878
BstSFI CTRYAG 1 cut(s) 697
BstUI CGCG 2 cut(s) 134, 264
BstV1I GCAGC 3 cut(s) 595, 834, 853
BstV2I GAAGAC 2 cut(s) 210, 1103
BstX2I RGATCY 2 cut(s) 739, 1127
BstYI RGATCY 2 cut(s) 739, 1127
BtgI CCRYGG 1 cut(s) 1074
BtgZI GCGATG 2 cut(s) 28, 1075
BtsCI GGATG 3 cut(s) 392, 541, 852
BtsI GCAGTG 1 cut(s) 185
BtsIMutI CAGTG 3 cut(s) 185, 408, 564
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 3 cut(s) 18, 581, 1058
CaiI CAGNNNCTG 1 cut(s) 879
CfoI GCGC 3 cut(s) 65, 95, 444
Cfr10I RCCGGY 1 cut(s) 417
Cfr13I GGNCC 3 cut(s) 472, 787, 876
Csp6I GTAC 2 cut(s) 619, 1019
CspAI ACCGGT 1 cut(s) 417
CviAII CATG 2 cut(s) 532, 1043
CviJI RGCY 8 cut(s) 86, 197, 347, 540, 586, 611, 686, 861
CviKI_1 RGCY 8 cut(s) 86, 197, 347, 540, 586, 611, 686, 861
CviQI GTAC 2 cut(s) 619, 1019
DdeI CTNAG 3 cut(s) 857, 1095, 1149
DpnI GATC 8 cut(s) 146, 184, 243, 436, 498, 741, 998, 1129
DpnII GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
Eam1104I CTCTTC 3 cut(s) 227, 386, 558
EarI CTCTTC 3 cut(s) 227, 386, 558
Eco130I CCWWGG 1 cut(s) 768
Eco31I GGTCTC 1 cut(s) 919
Eco47I GGWCC 3 cut(s) 472, 787, 876
Eco57I CTGAAG 2 cut(s) 222, 1097
Eco91I GGTNACC 1 cut(s) 413
EcoO109I RGGNCCY 2 cut(s) 787, 876
EcoO65I GGTNACC 1 cut(s) 413
EcoRII CCWGG 2 cut(s) 667, 878
EcoT14I CCWWGG 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 2 cut(s) 535, 1046
FaqI GGGAC 1 cut(s) 458
FatI CATG 2 cut(s) 531, 1042
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 132, 584, 823, 867
FokI GGATG 3 cut(s) 399, 548, 839
Fsp4HI GCNGC 4 cut(s) 132, 584, 823, 867
FspBI CTAG 1 cut(s) 513
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 3 cut(s) 64, 94, 443
GluI GCNGC 4 cut(s) 132, 584, 823, 867
GsuI CTGGAG 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 96
HapII CCGG 5 cut(s) 11, 194, 288, 329, 418
HhaI GCGC 3 cut(s) 65, 95, 444
Hin1II CATG 2 cut(s) 535, 1046
Hin6I GCGC 3 cut(s) 63, 93, 442
HinP1I GCGC 3 cut(s) 63, 93, 442
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 4 cut(s) 28, 249, 267, 935
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 5 cut(s) 11, 194, 288, 329, 418
HphI GGTGA 2 cut(s) 302, 407
Hpy166II GTNNAC 3 cut(s) 408, 529, 1040
Hpy188I TCNGA 9 cut(s) 74, 175, 182, 202, 214, 360, 760, 909, 1116
Hpy188III TCNNGA 5 cut(s) 25, 329, 719, 874, 1008
Hpy8I GTNNAC 3 cut(s) 408, 529, 1040
Hpy99I CGWCG 2 cut(s) 62, 101
HpyAV CCTTC 2 cut(s) 64, 1005
HpyCH4III ACNGT 5 cut(s) 452, 472, 568, 701, 1075
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 4 cut(s) 155, 583, 965, 1056
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 858
HpyF3I CTNAG 3 cut(s) 857, 1095, 1149
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 2 cut(s) 535, 1046
HspAI GCGC 3 cut(s) 63, 93, 442
Kpn2I TCCGGA 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 241
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 3 cut(s) 595, 834, 853
LweI GCATC 4 cut(s) 142, 702, 861, 952
MaeI CTAG 1 cut(s) 513
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 299, 413
MalI GATC 8 cut(s) 146, 184, 243, 436, 498, 741, 998, 1129
MboI GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
MflI RGATCY 2 cut(s) 739, 1127
MhlI GDGCHC 1 cut(s) 22
MluCI AATT 3 cut(s) 282, 592, 986
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 3 cut(s) 198, 408, 469
MnlI CCTC 6 cut(s) 16, 80, 188, 289, 561, 778
MroI TCCGGA 1 cut(s) 328
MseI TTAA 5 cut(s) 320, 528, 591, 705, 1088
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 5 cut(s) 11, 194, 288, 329, 418
MspR9I CCNGG 2 cut(s) 669, 880
MvaI CCWGG 2 cut(s) 669, 880
MvnI CGCG 2 cut(s) 134, 264
MwoI GCNNNNNNNGC 2 cut(s) 92, 858
NdeII GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
NlaIII CATG 2 cut(s) 535, 1046
NlaIV GGNNCC 2 cut(s) 474, 902
NmuCI GTSAC 1 cut(s) 413
NsbI TGCGCA 1 cut(s) 443
NspI RCATGY 1 cut(s) 1046
PciI ACATGT 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 28, 249, 935
PinAI ACCGGT 1 cut(s) 417
PkrI GCNGC 4 cut(s) 133, 585, 824, 868
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PpuMI RGGWCCY 2 cut(s) 787, 876
PscI ACATGT 1 cut(s) 1042
PshBI ATTAAT 1 cut(s) 591
Psp5II RGGWCCY 2 cut(s) 787, 876
Psp6I CCWGG 2 cut(s) 667, 878
PspEI GGTNACC 1 cut(s) 413
PspGI CCWGG 2 cut(s) 667, 878
PspN4I GGNNCC 2 cut(s) 474, 902
PspPI GGNCC 3 cut(s) 472, 787, 876
PspPPI RGGWCCY 2 cut(s) 787, 876
PstNI CAGNNNCTG 1 cut(s) 879
PsuI RGATCY 2 cut(s) 739, 1127
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 2 cut(s) 620, 1020
RsaNI GTAC 2 cut(s) 619, 1019
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 5 cut(s) 320, 528, 591, 705, 1088
SatI GCNGC 4 cut(s) 132, 584, 823, 867
Sau3AI GATC 8 cut(s) 144, 182, 241, 434, 496, 739, 996, 1127
Sau96I GGNCC 3 cut(s) 472, 787, 876
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 2 cut(s) 669, 880
SduI GDGCHC 1 cut(s) 22
SfaNI GCATC 4 cut(s) 142, 702, 861, 952
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 3 cut(s) 472, 787, 876
SmlI CTYRAG 2 cut(s) 110, 541
SmoI CTYRAG 2 cut(s) 110, 541
Sse9I AATT 3 cut(s) 282, 592, 986
SsiI CCGC 3 cut(s) 132, 264, 557
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 2 cut(s) 667, 878
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 5 cut(s) 452, 472, 568, 701, 1075
TaiI ACGT 1 cut(s) 318
TaqI TCGA 3 cut(s) 57, 506, 945
TaqII GACCGA 1 cut(s) 943
TasI AATT 3 cut(s) 282, 592, 986
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 28, 249, 935
Tru1I TTAA 5 cut(s) 320, 528, 591, 705, 1088
Tru9I TTAA 5 cut(s) 320, 528, 591, 705, 1088
TscAI CASTG 3 cut(s) 192, 415, 571
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 3 cut(s) 583, 822, 866
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 2 cut(s) 966, 984
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 3 cut(s) 192, 415, 571
VpaK11BI GGWCC 3 cut(s) 472, 787, 876
VspI ATTAAT 1 cut(s) 591
XapI RAATTY 1 cut(s) 282
XceI RCATGY 1 cut(s) 1046
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.