pycom09g10010

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002439
Physical Location & Seq
Forward (+)
8085632 .. 8088520
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10010.4

Sequence Viewer

Length: 1461 bp
ATGGATGACCCTCCAAGGGGTGTAAGTTTTTACCAAATTACCGTATCAACTGAATTACGAATTAGAATCGTACATTTTCAGTATGATAGTTTGCGCACAACTAATCAAAATGATGAATTACTAAATCACCCATGTCTCCGACGAAATATACTAAAAGACCACCACTTCCCGAAAACTCTCTCACCGTCTCCCCTCCAGCCCGCCACTCCACCAGTAGCGGCGCCAAATCACCGCCTTCTCTTCTCACACCGTTTCCCCTTTAATACATGTAGAACCCAAACGACAATCATCGCAATGTCGTTGCACGAGCGAGCCGTAATCAACCTAGTCTCCCAACTCGCCCTCTCCTGCGACGGCGCTGTTTTGGGCGTAGTCCTAGCGTGCGCTGCCGTGCGTACCGTCATCAAGTTCGCCTACACCTCCTCAGCCCTGCGCAAGCTCCGTAACGCTCCCTACGTCAAAGTCTCCGACCTCCGCGCAATCCTCGCCCCCGACGAATCTCAGCCCTCAGATGCAAAGCTCGTCGTCTTACGCGGCACCGTCGAAGCCAAGTCCGCCGTCGACGGCAAGTCGAATAGCCTCAAATCCGGCGTATTCGTCTCTCGGGGAACAAAGAACAGTGCCGTCGTCGTTCAGAGAACTCAAACGTGTGTTTATAAGGATCGGAAGAACTTCATCGGATTTCCGTTTGATTTGAGAAGTTTAATTTTGAAGCCTTGGAGAGAGCAAGAATCGAAGGATTTAAGAACGGTTCCTTTTGTTCTTGTTGAAGGTGGTCGAAGGAGGGTTTCGGAATTTGTTGTTGTGAACGTAGATGAATCAAGACATCCCTTACCTCTTACTACAGTTTATCGTAATTTTCATCCTGTTAATGCTTCTCCCTACAAATATAAACTCGTTGATGCACTCTTCCGGCATGAATATCCGATTGGTTTTCTTGATGAAGAGAAGATTCTTCCATTGGGAAAGGAAATTACCGCTGTCGGTCTCTGCAGTTTAAAAAATGGAGTGCCGGATATCAAGTCGTGCAAAGATCTTCCCTATTTTCTCTCTGAGATGTCTAAGGATGAGATGGTTGTAGAACTTACTTCACACACAAGAGTGCTGTTCTGGAGTGGGATTGTTCTTGTTTCAATGTCAATTGGAATCCTTGGCTATGCTGTTTCGAGGAATTGGGATAAATTGAAAGCGTGGAAGCAAGGAAGGCAGTCACAGCAATCAATCCCTGCTGCTGACGGCAATCCCGAAACTCAAATTGAAGAAGAGGAGGAAGTAGGAGAGGTTCCAGATGGACAGTTGTGTGTCATATGTCTGATGAGGAGAAGGCGATCGGCATTCACTCCTTGCGGGCATCGTGTTTGCTGCCACCCGTGTAGCATATCAATCCAACGGAATGTAACACCATGCTGTCCTGTTTGCCGGCAGGATATCCGCGCTTCAGTGCGAATATATGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0001558 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005777 GO:0006464 GO:0006807 GO:0006919 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009893 GO:0009941 GO:0009966 GO:0009967 GO:0009987 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0016020 GO:0016021 GO:0016043 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019867 GO:0023051 GO:0023056 GO:0030162 GO:0030308 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031323 GO:0031325 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032268 GO:0032270 GO:0032446 GO:0032592 GO:0036211 GO:0040008 GO:0042579 GO:0042802 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043122 GO:0043123 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043280 GO:0043281 GO:0043412 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045862 GO:0045926 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051646 GO:0052547 GO:0052548 GO:0060255 GO:0065007 GO:0065009 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0098573 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000116 GO:2001056
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

487

Amino Acids

54.6

Weight (kDa)

9.2

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
Acc16I TGCGCA 2 cut(s) 95, 434
AccB1I GGYRCC 2 cut(s) 220, 536
AccI GTMKAC 1 cut(s) 561
AccII CGCG 3 cut(s) 477, 534, 1434
AciI CCGC 9 cut(s) 201, 218, 232, 475, 534, 555, 978, 1347, 1432
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 1 cut(s) 794
AcuI CTGAAG 1 cut(s) 1422
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 2 cut(s) 72, 397
AfiI CCNNNNNNNGG 2 cut(s) 16, 17
AflIII ACRYGT 2 cut(s) 266, 647
AgsI TTSAA 5 cut(s) 712, 770, 1134, 1186, 1259
AhdI GACNNNNNGTC 1 cut(s) 568
AjuI GAANNNNNNNTTGG 2 cut(s) 912, 944
AleI CACNNNNGTG 1 cut(s) 1100
AluBI AGCT 2 cut(s) 439, 520
AluI AGCT 2 cut(s) 439, 520
Alw26I GTCTC 6 cut(s) 140, 192, 334, 469, 604, 992
AlwI GGATC 1 cut(s) 669
Ama87I CYCGRG 1 cut(s) 603
ApeKI GCWGC 3 cut(s) 386, 1229, 1362
ApoI RAATTY 1 cut(s) 794
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 7 cut(s) 96, 223, 359, 386, 435, 479, 1436
AsuHPI GGTGA 3 cut(s) 119, 174, 221
AvaI CYCGRG 1 cut(s) 603
BanI GGYRCC 2 cut(s) 220, 536
BauI CACGAG 1 cut(s) 305
BbvCI CCTCAGC 1 cut(s) 424
BbvI GCAGC 3 cut(s) 373, 1216, 1349
BccI CCATC 2 cut(s) 1066, 1283
BceAI ACGGC 7 cut(s) 299, 370, 374, 542, 580, 608, 1252
BcoDI GTCTC 6 cut(s) 140, 192, 334, 469, 604, 992
BfaI CTAG 2 cut(s) 326, 377
BfmI CTRYAG 2 cut(s) 843, 991
BfoI RGCGCY 2 cut(s) 224, 360
BglII AGATCT 1 cut(s) 1033
BisI GCNGC 5 cut(s) 219, 387, 535, 1230, 1363
BlsI GCNGC 5 cut(s) 220, 388, 536, 1231, 1364
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 603
BmiI GGNNCC 4 cut(s) 222, 538, 753, 1284
BmsI GCATC 3 cut(s) 502, 892, 1360
BpmI CTGGAG 2 cut(s) 179, 1132
Bpu10I CCTNAGC 1 cut(s) 424
BsaHI GRCGYC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 992
BsaJI CCNNGG 3 cut(s) 14, 716, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 17
Bse118I RCCGGY 1 cut(s) 1419
Bse1I ACTGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 300
BseDI CCNNGG 3 cut(s) 14, 716, 1150
BseGI GGATG 4 cut(s) 10, 826, 862, 1072
BseLI CCNNNNNNNGG 2 cut(s) 16, 17
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 4 cut(s) 438, 515, 522, 1044
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 3 cut(s) 412, 1280, 1333
BseXI GCAGC 3 cut(s) 373, 1216, 1349
Bsh1236I CGCG 3 cut(s) 477, 534, 1434
Bsh1285I CGRYCG 1 cut(s) 1331
BshNI GGYRCC 2 cut(s) 220, 536
BsiEI CGRYCG 1 cut(s) 1331
BsiHKCI CYCGRG 1 cut(s) 603
BsiSI CCGG 4 cut(s) 588, 913, 1013, 1420
BslI CCNNNNNNNGG 2 cut(s) 16, 17
BsmAI GTCTC 6 cut(s) 140, 192, 334, 469, 604, 992
BsmBI CGTCTC 2 cut(s) 192, 604
BsmI GAATGC 1 cut(s) 1334
Bso31I GGTCTC 1 cut(s) 992
BsoBI CYCGRG 1 cut(s) 603
Bsp143I GATC 3 cut(s) 661, 1033, 1328
BspACI CCGC 9 cut(s) 201, 218, 232, 475, 534, 555, 978, 1347, 1432
BspCNI CTCAG 4 cut(s) 437, 514, 521, 1045
BspFNI CGCG 3 cut(s) 477, 534, 1434
BspLI GGNNCC 4 cut(s) 222, 538, 753, 1284
BspMAI CTGCAG 1 cut(s) 995
BspPI GGATC 1 cut(s) 669
BspT107I GGYRCC 2 cut(s) 220, 536
BspTNI GGTCTC 1 cut(s) 992
BsrDI GCAATG 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 1419
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 1419
BssECI CCNNGG 3 cut(s) 14, 716, 1150
BssMI GATC 3 cut(s) 661, 1033, 1328
BssNI GRCGYC 1 cut(s) 221
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 3 cut(s) 14, 716, 1150
Bst2BI CACGAG 1 cut(s) 305
Bst4CI ACNGT 9 cut(s) 43, 186, 251, 400, 541, 620, 751, 847, 1296
Bst6I CTCTTC 4 cut(s) 245, 914, 939, 1257
BstACI GRCGYC 1 cut(s) 221
BstC8I GCNNGC 6 cut(s) 201, 312, 382, 437, 1349, 1421
BstDEI CTNAG 5 cut(s) 424, 501, 508, 1053, 1062
BstF5I GGATG 4 cut(s) 10, 826, 862, 1072
BstFNI CGCG 3 cut(s) 477, 534, 1434
BstH2I RGCGCY 2 cut(s) 224, 360
BstHHI GCGC 7 cut(s) 96, 223, 359, 386, 435, 479, 1436
BstKTI GATC 3 cut(s) 664, 1036, 1331
BstMAI GTCTC 6 cut(s) 140, 192, 334, 469, 604, 992
BstMBI GATC 3 cut(s) 661, 1033, 1328
BstMCI CGRYCG 1 cut(s) 1331
BstMWI GCNNNNNNNGC 5 cut(s) 386, 485, 554, 1204, 1213
BstNSI RCATGY 1 cut(s) 270
BstSFI CTRYAG 2 cut(s) 843, 991
BstUI CGCG 3 cut(s) 477, 534, 1434
BstV1I GCAGC 3 cut(s) 373, 1216, 1349
BstX2I RGATCY 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 1033
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 4 cut(s) 10, 826, 862, 1072
BtsIMutI CAGTG 2 cut(s) 625, 1446
Cac8I GCNNGC 6 cut(s) 201, 312, 382, 437, 1349, 1421
CfoI GCGC 7 cut(s) 96, 223, 359, 386, 435, 479, 1436
Cfr10I RCCGGY 1 cut(s) 1419
Csp6I GTAC 2 cut(s) 71, 396
CviAII CATG 4 cut(s) 132, 267, 917, 1404
CviQI GTAC 2 cut(s) 71, 396
DdeI CTNAG 5 cut(s) 424, 501, 508, 1053, 1062
DinI GGCGCC 1 cut(s) 222
DpnI GATC 3 cut(s) 663, 1035, 1330
DpnII GATC 3 cut(s) 661, 1033, 1328
DraI TTTAAA 1 cut(s) 999
DriI GACNNNNNGTC 1 cut(s) 568
Eam1104I CTCTTC 4 cut(s) 245, 914, 939, 1257
Eam1105I GACNNNNNGTC 1 cut(s) 568
EarI CTCTTC 4 cut(s) 245, 914, 939, 1257
EciI GGCGGA 1 cut(s) 544
Eco130I CCWWGG 3 cut(s) 14, 716, 1150
Eco31I GGTCTC 1 cut(s) 992
Eco32I GATATC 2 cut(s) 1018, 1429
Eco57I CTGAAG 1 cut(s) 1422
Eco88I CYCGRG 1 cut(s) 603
EcoRV GATATC 2 cut(s) 1018, 1429
EcoT14I CCWWGG 3 cut(s) 14, 716, 1150
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 3 cut(s) 14, 716, 1150
Esp3I CGTCTC 2 cut(s) 192, 604
FaeI CATG 4 cut(s) 135, 270, 920, 1407
FatI CATG 4 cut(s) 131, 266, 916, 1403
FauI CCCGC 2 cut(s) 208, 1340
FauNDI CATATG 1 cut(s) 1307
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 5 cut(s) 219, 387, 535, 1230, 1363
FokI GGATG 4 cut(s) 17, 813, 849, 1079
Fsp4HI GCNGC 5 cut(s) 219, 387, 535, 1230, 1363
FspBI CTAG 2 cut(s) 326, 377
FspI TGCGCA 2 cut(s) 95, 434
GlaI GCGC 7 cut(s) 95, 222, 358, 385, 434, 478, 1435
GluI GCNGC 5 cut(s) 219, 387, 535, 1230, 1363
GsuI CTGGAG 2 cut(s) 179, 1132
HaeII RGCGCY 2 cut(s) 224, 360
HapII CCGG 4 cut(s) 588, 913, 1013, 1420
HhaI GCGC 7 cut(s) 96, 223, 359, 386, 435, 479, 1436
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 4 cut(s) 135, 270, 920, 1407
Hin6I GCGC 7 cut(s) 94, 221, 357, 384, 433, 477, 1434
HinP1I GCGC 7 cut(s) 94, 221, 357, 384, 433, 477, 1434
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 7 cut(s) 66, 497, 731, 818, 952, 1146, 1454
HpaII CCGG 4 cut(s) 588, 913, 1013, 1420
HphI GGTGA 3 cut(s) 119, 174, 221
Hpy166II GTNNAC 2 cut(s) 562, 808
Hpy188III TCNNGA 6 cut(s) 169, 822, 938, 1111, 1244, 1286
Hpy8I GTNNAC 2 cut(s) 562, 808
Hpy99I CGWCG 9 cut(s) 144, 356, 497, 527, 545, 563, 566, 629, 632
HpyAV CCTTC 6 cut(s) 245, 730, 764, 774, 1197, 1317
HpyCH4III ACNGT 9 cut(s) 43, 186, 251, 400, 541, 620, 751, 847, 1296
HpyCH4IV ACGT 3 cut(s) 456, 647, 810
HpyCH4V TGCA 5 cut(s) 304, 515, 905, 993, 1029
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 485, 554, 1204, 1213
HpyF3I CTNAG 5 cut(s) 424, 501, 508, 1053, 1062
HpySE526I ACGT 3 cut(s) 456, 647, 810
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 4 cut(s) 135, 270, 920, 1407
HspAI GCGC 7 cut(s) 94, 221, 357, 384, 433, 477, 1434
KasI GGCGCC 1 cut(s) 220
KroI GCCGGC 1 cut(s) 1419
KroNI GCCGGC 1 cut(s) 1421
Kzo9I GATC 3 cut(s) 661, 1033, 1328
LmnI GCTCC 2 cut(s) 444, 454
Lsp1109I GCAGC 3 cut(s) 373, 1216, 1349
LweI GCATC 3 cut(s) 502, 892, 1360
MaeI CTAG 2 cut(s) 326, 377
MaeII ACGT 3 cut(s) 456, 647, 810
MaeIII GTNAC 3 cut(s) 443, 1209, 1396
MalI GATC 3 cut(s) 663, 1035, 1330
MboI GATC 3 cut(s) 661, 1033, 1328
MboII GAAGA 9 cut(s) 232, 679, 901, 947, 956, 961, 1028, 1271, 1274
MfeI CAATTG 1 cut(s) 1140
MflI RGATCY 1 cut(s) 1033
Mly113I GGCGCC 1 cut(s) 221
MmeI TCCRAC 3 cut(s) 163, 492, 1411
MroNI GCCGGC 1 cut(s) 1419
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 6 cut(s) 261, 704, 743, 870, 998, 1459
MslI CAYNNNNRTG 2 cut(s) 293, 1100
MspA1I CMGCKG 1 cut(s) 980
MspI CCGG 4 cut(s) 588, 913, 1013, 1420
MunI CAATTG 1 cut(s) 1140
Mva1269I GAATGC 1 cut(s) 1334
MvnI CGCG 3 cut(s) 477, 534, 1434
MwoI GCNNNNNNNGC 5 cut(s) 386, 485, 554, 1204, 1213
NaeI GCCGGC 1 cut(s) 1421
NarI GGCGCC 1 cut(s) 221
NdeI CATATG 1 cut(s) 1307
NdeII GATC 3 cut(s) 661, 1033, 1328
NgoMIV GCCGGC 1 cut(s) 1419
NlaIII CATG 4 cut(s) 135, 270, 920, 1407
NlaIV GGNNCC 4 cut(s) 222, 538, 753, 1284
NmuCI GTSAC 1 cut(s) 1209
NsbI TGCGCA 2 cut(s) 95, 434
NspI RCATGY 1 cut(s) 270
OliI CACNNNNGTG 1 cut(s) 1100
PciI ACATGT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 4 cut(s) 312, 453, 492, 1242
PctI GAATGC 1 cut(s) 1334
PdiI GCCGGC 1 cut(s) 1421
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 7 cut(s) 66, 497, 731, 818, 952, 1146, 1454
PkrI GCNGC 5 cut(s) 220, 388, 536, 1231, 1364
Ple19I CGATCG 1 cut(s) 1331
PluTI GGCGCC 1 cut(s) 224
PscI ACATGT 1 cut(s) 266
PsiI TTATAA 1 cut(s) 657
PspN4I GGNNCC 4 cut(s) 222, 538, 753, 1284
PsrI GAACNNNNNNTAC 2 cut(s) 1266, 1298
PstI CTGCAG 1 cut(s) 995
PsuI RGATCY 1 cut(s) 1033
PvuI CGATCG 1 cut(s) 1331
RsaI GTAC 2 cut(s) 72, 397
RsaNI GTAC 2 cut(s) 71, 396
RseI CAYNNNNRTG 2 cut(s) 293, 1100
SalI GTCGAC 1 cut(s) 560
SaqAI TTAA 6 cut(s) 261, 704, 743, 870, 998, 1459
SatI GCNGC 5 cut(s) 219, 387, 535, 1230, 1363
Sau3AI GATC 3 cut(s) 661, 1033, 1328
SfaNI GCATC 3 cut(s) 502, 892, 1360
SfcI CTRYAG 2 cut(s) 843, 991
SfoI GGCGCC 1 cut(s) 222
SgrDI CGTCGACG 1 cut(s) 560
SmiMI CAYNNNNRTG 2 cut(s) 293, 1100
SsiI CCGC 9 cut(s) 201, 218, 232, 475, 534, 555, 978, 1347, 1432
SspDI GGCGCC 1 cut(s) 220
SspMI CTAG 2 cut(s) 326, 377
StyI CCWWGG 3 cut(s) 14, 716, 1150
TaaI ACNGT 9 cut(s) 43, 186, 251, 400, 541, 620, 751, 847, 1296
TaiI ACGT 3 cut(s) 459, 650, 813
TaqI TCGA 6 cut(s) 543, 561, 572, 734, 778, 1166
TaqII GACCGA 1 cut(s) 974
TauI GCSGC 2 cut(s) 221, 537
TfiI GAWTC 7 cut(s) 66, 497, 731, 818, 952, 1146, 1454
Tru1I TTAA 6 cut(s) 261, 704, 743, 870, 998, 1459
Tru9I TTAA 6 cut(s) 261, 704, 743, 870, 998, 1459
TscAI CASTG 2 cut(s) 625, 1446
TseFI GTSAC 1 cut(s) 1209
TseI GCWGC 3 cut(s) 386, 1229, 1362
Tsp45I GTSAC 1 cut(s) 1209
TspDTI ATGAA 6 cut(s) 129, 664, 831, 851, 933, 957
TspGWI ACGGA 3 cut(s) 431, 675, 1405
TspRI CASTG 2 cut(s) 625, 1446
XapI RAATTY 1 cut(s) 794
XceI RCATGY 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 561
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 2 cut(s) 326, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.